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Virologica Sinica 2023年第1期38卷 162-164页

作者： Lei Yang Yingshan Chen Sha Li Yuan zhou Yongli Zhang rongjuan Pei Xinwen Chen Yun Wang State Key Laboratory of Virology Center for Biosafety Mega-ScienceWuhan Institute of VirologyChinese Academy of SciencesWuhan430071China University of Chinese Academy of Sciences Beijing100049China Innovation Center for Pathogen Research Guangzhou LaboratoryGuangzhou510320China

Dear Editor,Since the global eradication of smallpox in the 1980s,monkeypox has become the most severe infectious disease caused by pathogenic orthopoxvirus(OPXV)*** virus(MPXV)is the causative pathogen of monkeypox and was classified as a category of human infectious pathogen by theMinistry of Health of the People's Republic ofChina in *** has traditionally been endemic in West and Central Africa since it was discovered nearly 70 years ago(Breman et al.,1980).However,beginning in early May 2022,a sudden outbreak of monkeypox has taken place and remains ongoing in more than 100 nonendemic countries and territories located at Europe,Americas,and Asia(https://***/mpx_global/).

关键词： protective traditionally monkey

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高铁开通对长三角地区旅游业发展的影响研究

西南大学学报（自然科学版） 2025年第2期47卷 106-121页

作者：时朋飞古俊龙黄荣娟张鸿浩李星明西南大学经济管理学院重庆400715 河池学院商学院广西宜州546300 华中师范大学城市与环境科学学院武汉430079

在系统阐述高铁开通与旅游业发展内在关联的基础之上,基于2010-2019年长三角地区41个城市的面板数据,运用倾向得分匹配法与多期渐进倍差法评估高铁开通对长三角地区城市旅游业发展数量和质量的影响。研究结果表明:①基于整体视角,高铁... 详细信息

在系统阐述高铁开通与旅游业发展内在关联的基础之上,基于2010-2019年长三角地区41个城市的面板数据,运用倾向得分匹配法与多期渐进倍差法评估高铁开通对长三角地区城市旅游业发展数量和质量的影响。研究结果表明:①基于整体视角,高铁开通促进了长三角地区城市旅游业发展数量与质量的双提升,对发展质量的影响程度大于对发展数量的影响程度,同时高铁开通对该地区城市旅游业存在既“旺财”又“旺丁”的效果;②基于异质性视角,高铁开通对新一线及以上城市旅游业发展质量有微弱正向作用,但无法助推其发展数量的提升,对二线城市旅游业发展数量影响强而对发展质量影响较弱,对三线、四线及以下城市旅游业发展质量的提升影响较大而对发展数量影响微弱,这类城市沦为高铁旅游的“过道”;③基于驱动力视角,技术进步成为该地区旅游业全要素生产率提升的核心动力,同时高铁开通对除四线及以下城市的发展层次城市技术进步的驱动作用强于对技术效率的驱动作用。据此,在加快旅游强国建设背景下,应将高铁建设纳入推进区域旅游业高质量发展的有效手段和政策工具,通过高铁网络完善产生的杠杆效应进一步促进区域旅游一体化建设、旅游技术创新与扩散、技术效率改进与提升。

关键词：旅游业发展高铁开通倾向得分匹配法多期渐进倍差法长三角地区

Research on market-based policy instruments for eco-environmental protection

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Chinese Journal of Population,Resources and Environment 2013年第2期11卷 149-154页

作者： rongjuan Li Youxiang Sun School of Politics & Law and Public Administration Hubei University

The selection of policy instruments involves policy efficiency and policy *** settlement of eco-environment problems in the process of industrialization and implementation of active eco-environment protection depend on the innovation of policy *** this paper,based on a descriptive analysis of the policy instruments for ecoenvironmental protection of our country,combined with the theoretical analysis and the empirical research on current marketization of eco-environmental protection,the authors indicate that eco-environmental problems originate fi'om the damage of eco-environment and the extemalities of eco-service,and that the market-based policy instruments which are helpful to balance the relationship between fight,responsibility and interest in the eco-service are effective measures to eliminate externalities and protect eco-environment.

关键词： eco-environmental protection eco-compensation market-based policy instruments

Effects of main ecological factors on the growth of marine green alga Caulerpa sertularioides using the response surface methodology

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Acta Oceanologica Sinica 2023年第11期42卷 90-97页

作者： Bingxin Huang Yue Chu rongjuan Wang Yixiao Wang Lanping Ding College of Life Sciences Tianjin Normal UniversityTianjin 300387China Tianjin Key Laboratory of Animal and Plant Resistance Tianjin 300387China

Caulerpa sertularioides is an invasive potential blooming green alga in China but it remains poorly *** studied the effects of ecological factors on its *** conditions of ecological factors,i.e.,irradiance,temperature,and salinity,for the growth of its fragments were determined in the response surface methodology(RSM).The specific growth rates(SGR)of the fragments were determined in single-factor *** results show that the SGR of *** peaked under the conditions of irradiance 37.5μmol/(m~2·s),temperature25℃,and salinity ***,using the Box-Behnken design,the conditions were further optimized and verified to be:irradiance 39.03μmol/(m~2·s),temperature 25.29℃,and salinity 30.06,under which the SGR reached 4.66%.The results provide new theoretical data and solutions for the cultivation,invasion prediction,and monitoring of Caulerpa species in China and the *** RSM method may have great potential applications in the environmental adaptation characteristics of new macroalgal cultivars,intensive orientation cultured germplasm,and environmental hazard analysis of cultivated species in the field.

关键词： Caulerpa sertularioides fragment response surface methodology(RSM) ecological factor

Phosphatidylserine-Specific Phospholipase A1 is the Critical Bridge for Hepatitis C Virus Assembly

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Virologica Sinica 2019年第5期34卷 521-537页

作者： Qi Yang Min Guo Yuan zhou Xue Hu Yun Wang Chunchen Wu Min Yang rongjuan Pei Xinwen Chen Jizheng Chen Department of Gastroenterology Guangzhou Women and Children's Medical CenterGuangzhou 510623China State Key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China School of Life Science and Technology China Pharmaceutical UniversityNanjing 210009China

The phosphatidylserine-specific phospholipase A1(PLA1A)is an essential host factor in hepatitis C virus(HCV)*** this study,we mapped the E2,NS2 and NS5A involved in PLA1A interaction to their lumenal domains and membranous parts,through which they form oligomeric protein complexes to participate in HCV *** regions of PLA1A were involved in their interaction and complex ***,the results represented structures with PLA1A and E2 in closer proximity than NS2 and NS5A,and strongly suggest PLA1 A-E2,s physical interaction in ***,we mapped the NS5A sequence which participated in PLA1A interaction with the C-terminus of domain ***,these amino acids in the sequence are also essential for viral RNA *** experiments revealed that these four proteins interact with each ***,PLA1A expression levels were elevated in livers from HCV-infected *** conclusion,we exposed the structural determinants of PLA1A,E2,NS2 and NS5A proteins which were important for HCV assembly and provided a detailed characterization of PLA1A in HCV assembly.

关键词： Phosphatidylserine-specific phospholipase A1(PLA1 A) HCV assembly Viral RNA replication

Different Responses of Two Highly Permissive Cell Lines Upon HCV Infection

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Virologica Sinica 2013年第4期28卷 202-208页

作者： Honghe Chen rongjuan Pei Xinwen Chen State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences University of Chinese Academy of Sciences

The construction of the first infectious clone JFH-1 speeds up the research on hepatitis C virus (HCV). However, Huh7 cell line was the only highly permissive cell line for HCV infection and only a few clones were fully permissive. In this study, two different fully permissive clones of Huh7 cells, Huh7.5.1 and Huh7-Lunet-CD81 (Lunet-CD81) cells were compared for their responses upon HCV infection. The virus replication level was found slightly higher in Huh7.5.1 cells than that in Lunet-CD81 cells. Viability of Huh7.5.1 cells but not of Lunet-CD81 cells was reduced significantly after HCV infection. Further analysis showed that the cell cycle of infected Huh7.5.1 cells was arrested at G1 phase. The G1/S transition was blocked by HCV infection in Huh7.5.1 cells as shown by the cell cycle synchronization analysis. Genes related to cell cycle regulation was modified by HCV infection and gene interaction analysis in GeneSpring GX in Direct Interactions mode highlighted 31 genes. In conclusion, the responses of those two cell lines were different upon HCV infection. HCV infection blocked G1/S transition and cell cycle progress, thus reduced the cell viability in Huh7.5.1 cells but not in Lunet-CD81 cells. Lunet-CD81 cells might be suitable for long term infection studies of HCV.

关键词： HCV Huh7.5.1 Huh7-Lunet-CD81 Viability Cell cycle G1/S

Hepatitis C virus-induced prion protein expression facilitates hepatitis C virus replication

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Virologica Sinica 2017年第6期32卷 503-510页

作者： Huixia Zhang Shanshan Gao rongjuan Pei Xinwen Chen Chaoyang Li State Key Laboratory of Virology Center for Molecular Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of the Chinese Academy of Sciences Beijing 100000China

Hepatitis C virus(HCV) infects approximately 180 million people worldwide. Significant progress has been made since the establishment of in vitro HCV infection models in cells. However, the replication of HCV is complex and not completely understood. Here, we found that the expression of host prion protein(Pr P) was induced in an HCV replication cell model. We then showed that increased Pr P expression facilitated HCV genomic replication. Finally, we demonstrated that the KKRPK motif on the N-terminus of Pr P bound nucleic acids and facilitated HCV genomic replication. Our results provided important insights into how viruses may harness cellular protein to achieve propagation.

关键词： hepatitis C virus(HCV) NS3 NS5B prion protein(PrP)

Repurposing of Antazoline Hydrochloride as an Inhibitor of Hepatitis B Virus DNA Secretion

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Virologica Sinica 2021年第3期36卷 501-509页

作者： Jing Li Yangyang Hu Yifei Yuan Yinan Zhao Qiqi Han Canyu Liu Xue Hu Yuan zhou Yun Wang Yu Guo Chunchen Wu Xinwen Chen rongjuan Pei College of Pharmacy Nankai UniversityTianjin 300350China State Key Lab of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China Shanghai Public Health Clinical Center Fudan UniversityShanghai 201508China Guangzhou Institutes of Biomedicine and Health Chinese Academy of SciencesGuangzhou 510530China Clinical Laboratory Maternal and Child Health Hospital of Hubei ProvinceWuhan 430070China

Hepatitis B virus(HBV) belongs to Hepadnaviridae family and mainly infects hepatocytes, which can cause acute or chronic hepatitis. Currently, two types of antiviral drugs are approved for chronic infection clinically: interferons and nucleos(t)ide analogues. However, the clinical cure for chronic infection is still rare, and it is a huge challenge for all researchers to develop high-efficiency, safe, non-tolerant, and low-toxicity anti-HBV drugs. Antazoline hydrochloride is a first-generation antihistamine with anticholinergic properties, and it is commonly used to relieve nasal congestion and in eye drops. Recently, an in vitro high-throughput evaluation system was constructed to screen nearly 800 compounds from the Food and Drug Administration(FDA)-approved Drug Library. We found that arbidol hydrochloride and antazoline hydrochloride can effectively reduce HBV DNA in the extracellular supernatant in a dose-dependent manner, with EC_(50) of4.321 lmol/L and 2.910 lmol/L in HepAD38 cells, respectively. Moreover, the antiviral effects and potential mechanism of action of antazoline hydrochloride were studied in different HBV replication systems. The results indicate that antazoline hydrochloride also has a significant inhibitory effect on HBV DNA in the extracellular supernatant of Huh7 cells,with an EC_(50) of 2.349 lmol/L. These findings provide new ideas for screening and research related to HBV agents.

关键词： Antazoline hydrochloride Hepatitis B virus(HBV) High-throughput evaluation system HepAD38

Host HDAC4 regulates the antiviral response by inhibiting the phosphorylation of IRF3

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Journal of Molecular Cell Biology 2019年第2期11卷 158-169页

作者： Qi Yang Jielin Tang rongjuan Pei XiaoXiao Gao Jing Guo Chonghui Xu Yun Wang QianWang Chunchen Wu Yuan zhou Xue Hu He Zhao Yanyi Wang Xinwen Chen Jizheng Chen State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China University of Chinese Academy of Sciences Beijing 100049 China Key Laboratory of Human Functional Genomics of Jiangsu Province School of Basic Medical Science Nanjing Medical University Nanjing 210093 China

Class II HDACs, such as HDAC4, are critical regulators of the immune response in various immune cells;however, its role in innate immunity remains largely ***, we report that the overexpression of HDAC4 suppresses the production of type I interferons triggered by pattern-recognition receptors (PRRs). HDAC4 repressed the translocation of transcription factor IRF3 to the nucleus, thereby decreasing IRF3-mediated IFN-β expression. In particular, we also determined that HDAC4 can be phosphorylated and simultaneously block the phosphorylation of IRF3 at Ser386 and Ser396 by TBK1 and IKKε, respectively, by interacting with the kinase domain of TBK1 and IKKε. Furthermore, IFN-β may stimulate the expression of HDAC4. Our findings suggest that HDAC4 acts as a regulator of PRR signaling and is a novel mechanism of negative feedback regulation for preventing an overreactive innate immune response.

关键词： HDAC4 antiviral response IRF3 TBK1 IKKε

Ordering effects of cholesterol on sphingomyelin monolayers investigated by high-resolution broadband sum-frequency generation vibrational spectroscopy

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Chinese Chemical Letters 2018年第3期29卷 357-360页

作者： Yiyi Li rongjuan Feng Lu Lin Minghua Liu Yuan Guo Zhen Zhang Beijing National Laboratory for Molecular Sciences CAS Research/Education Center for Excellence in Molecular Sciences Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China University of Chinese Academy Sciences Beijing 100049 China National Center for Nanoscience and Technology Beijing 100190 China

This report investigated the ordering of the alky chain of sphingomyelin （SMs） monolayers induced by cholesterol at the air/water interface using high-resolution broadband sum frequency generation vibrational spectroscopy （HR-BB-SFG-VS）. The SFG spectra of the three nature sphingomyelin/cholesterol mixture monolayers with two concentrations of the cholesterol at the air/water interface are performed under different polarization combination. A new resolved CH2 symmetric stretching （d＋, ~2834 cm-1） and the CH3 symmetric stretching （r＋, ~2874 cm-1） mode are applied to characterize the conformational order in the sphingomyelin/cholesterol mixture monolayers. It was found that the cholesterol make the sphingosine backbones more conformational order. During this process, the conformational order of the N-linked acyl chain remains unaltered. Moreover, the sphingosine backbones of SMs have much larger contributions to gauche defects of SMs than one in the N-linked acyl chain. These results presented here not only shed lights on understanding of the interactions of sphingomyelin molecules with cholesterol molecules at interface but also demonstrates the ability of HR-BB-SFG to probe such complicated molecular systems.

关键词： Sphingomyelin Cholesterol Ordering effect Air/liquid interface High-resolution broadband sum frequency generation Vibrational spectroscopy