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Localization and Dynamic Expression of a 27.8kDa Receptor Protein for Lymphocystis Disease Virus Infection in Sea Bass(Lateolabrax japonicus) Tissues

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Journal of Ocean University of China 2017年第5期16卷 889-896页

作者： wu ronghua SHENG Xiuzhen TANG Xiaoqian XING Jing ZHAN Wenbin Laboratory of Pathology and Immunology of Aquatic Animals Ocean University of China Function Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and Technology

Lymphocystis disease virus(LCDV) infects target cells by attaching to a 27.8 k Da receptor(27.8R) protein in flounder Paralichthys olivaceus, and anti-27.8R monoclonal antibodies(MAbs) have been developed. However, the 27.8R existence in tissues of sea bass(Lateolabrax japonicus) and its role in LCDV infection have remained unclear. In this study, the results of western blotting demonstrated that the same 27.8R was shared by flounder and sea bass. LCDV-free sea bass individuals were intramuscularly injected with LCDV, and viral copies were detected in tissues from 3 h post infection and showed a time-dependent increase during 9 days infection. Distribution and synthesis of 27.8R in sea bass tissues were investigated by using anti-27.8R MAbs as probes. It was found that 27.8R was distributed in all the tested tissues. The levels of 27.8R protein were highest in gill and skin, then a bit lowly in stomach, head kidney and heart, followed by spleen, intestine, blood cells, gonad and liver, and least in kidney and brain in healthy sea bass. Upon LCDV infection, 27.8R synthesis was up-regulated in each tissue, and higher in the tissues with higher LCDV copies. The 27.8R and LCDV were detected in some peripheral blood leukocytes but not in red blood cells. These results suggested that 27.8R was widely distributed in sea bass tissues, and it served as a receptor and correlated with tissue tropism of LCDV infection. Furthermore, leukocytes had the potential of being a LCDV carrier and were responsible for a systemic infection of LCDV in sea bass.

关键词： Lateolabrax Infection intestine stomach Paralichthys monoclonal probes unclear Receptor Scophthalmus

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Dual-Branch-UNet: A Dual-Branch Convolutional Neural Network for Medical Image Segmentation

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Computer Modeling in Engineering & Sciences 2023年第10期137卷 705-716页

作者： Muwei Jian ronghua wu Hongyu Chen Lanqi Fu Chengdong Yang School of Information Science and Technology Linyi UniversityLinyi276000China School of Computer Science and Technology Shandong University of Finance and EconomicsJinan250014China School of Science&Engineering University of LimerickLimerickV94 T9PXIreland

In intelligent perception and diagnosis of medical equipment,the visual and morphological changes in retinal vessels are closely related to the severity of cardiovascular diseases(e.g.,diabetes and hypertension).Intelligent auxiliary diagnosis of these diseases depends on the accuracy of the retinal vascular segmentation *** address this challenge,we design a Dual-Branch-UNet framework,which comprises a Dual-Branch encoder structure for feature extraction based on the traditional U-Net model for medical image *** be more explicit,we utilize a novel parallel encoder made up of various convolutional modules to enhance the encoder portion of the original ***,image features are combined at each layer to produce richer semantic data and the model’s capacity is adjusted to various input ***,in the lower sampling section,we give up pooling and conduct the lower sampling by convolution operation to control step size for information *** also employ an attentionmodule in the decoder stage to filter the image noises so as to lessen the response of irrelevant *** are verified and compared on the DRIVE and ARIA datasets for retinal vessels *** proposed Dual-Branch-UNet has proved to be superior to other five typical state-of-the-art methods.

关键词： Convolutional neural network medical image processing retinal vessel segmentation

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A NOVEL DRIVING STRATEGY FOR DYNAMIC SIMULATION OF HOISTING ROPE WITH TIME-VARYING LENGTH

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International Journal of Modeling, Simulation, and Scientific Computing 2013年第3期4卷 54-70页

作者： JINJIE WANG GUOHUA CAO YANDONG WANG ronghua wu School of Mechatronic Engineering China University of Mining and Technology No.1 Da Xue Road XuzhouJiangsu Province 221116P.R.China

A simulation method for investigating the vibration behavior of hoisting rope with time-varying length is *** previously creating markers in the *** software package,the parametric model of the rope wound along helix is established based on the concentrated-mass theory with multi-degree of freedom(multi-DOF).A novel driving strategy,cooperating fixed joints with angle sensors under the control of driving script,is proposed to substitute conventional contact *** on the hoisting rope in the sinking winch mechanism,an equivalent discretization model is obtained with complicated boundary conditions *** differential equations of motion of the hoisting system are formulated employing Lagrange’s equation and numerically solved using Runge–Kutta *** simulation indicates that the horizontal swing is decreased in principle and the simulation with 800 discrete ropes is not performed more than 61 ***,this feasible strategy could not only guarantee the accuracy but also promote simulation efficiency and *** motion curves exported from ADAMS simulation coincide with one in numerical simulation,which validates both the numerical model and the driving strategy.

关键词： Winding modeling driving strategy MSC.ADAMS numerical validation.

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Long-Term Consistent Recalibration of VIRR Solar Reflectance Data Record for Fengyun Polar-Orbiting Satellites

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Journal of Meteorological Research 2021年第6期35卷 926-942页

作者： Ling SUN Hong QIU ronghua wu Jing WANG Liyang ZHANG Peng ZHANG National Satellite Meteorological Center China Meteorological AdministrationBeijing 100081

The visible infrared radiometer(VIRR)is the first instrument with longest measurements equipped on the Fengyun(FY)polar-orbiting *** re-processing of the historic VIRR measurements,long-term(over 20 yr)global data can be integrated from multiple participating VIRRs on a consistent radiometric scale,which are valuable to climate and climate change *** to lack of an onboard calibration system for VIRR,the reflective solar bands must be vicariously *** study applied the multi-site vicarious approach for consistent calibration of the VIRR visible(VIS)and near-infrared(NIR)data,and produced calibration coefficients for five VIRRs on FY-1 C/D and FY-3 A/B/*** data quality was then evaluated with *** reflectance predicted by using the radiative transfer model over multiple invariant desert and ocean targets was used to derive the calibration slope via a weighted fitting scheme,in which the weights are the inverse of the variance from a radiative transfer simulation evaluated with reference to Aqua moderate resolution imaging spectroradiometer(MODIS).The sensor-specific calibration coefficients were derived on a daily basis by using piecewise *** calibration reference of the VIRR solar band record was further traced to the Aqua MODIS Collection 6.1 reference calibration with a systematic correction derived from the Libya4 *** VIRR record was compared with the Aqua MODIS C6.1 calibration over the polar region based on simultaneous nadir overpass *** lifetime relative difference for each sensor are within 3.3%and 4.5%for channels 1 and *** deserts were also employed to evaluate the stability and consistency of the VIRR *** general,the means of the directional and spectral corrected reflectance for each sensor are within 1%of the 20-yr average,implying that the VIRR reflectance of the invariant targets is consistent to within 1%among the sensors for channels 1 and *** VIRR data th

关键词： FY-1 FY-3 visible infrared radiometer(VIRR) reflective solar bands pseudo-invariant calibration sites(PICS) calibration directional model(DM) spectral band adjustment factor(SBAF)

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Radiometric Cross-Calibration for Multiple Sensors with the Moon as an Intermediate Reference

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Journal of Meteorological Research 2019年第5期33卷 925-933页

作者： Lu ZHANG Peng ZHANG Xiuqing HU Lin CHEN Min MIN Na XU ronghua wu Nanjing University of Information Science&Technology Nanjing 210044 Chinese Academy of Meteorological Sciences China Meteorological AdministrationBeijing 100081 Key Laboratory of Radiometric Calibration and Validation for Environmental Satellites National Satellite Meteorological CenterChina Meteorological AdministrationBeijing 100081

The instrument cross-calibration is an effective way to assess the quality of satellite data. In this study, a new method is proposed to cross-calibrate the sensors among satellite instruments by using a RObotic Lunar Observatory(ROLO) model and Apollo sample reflectance in reflective solar bands(RSBs). The ROLO model acts as a transfer radiometer to bridge between the instruments. The reflective spectrum of the Apollo sample is used to compensate for the difference in the instrument's relative spectral responses(RSRs). In addition, the double ratio between the observed lunar irradiance and the simulated lunar irradiance is used to reduce the difference in instrument lunar viewing and illumining geometry. This approach is applied to the Moderate Resolution Imaging Spectroradiometer(MODIS), the Sea-Viewing Wide Field-of-View Sensor(Sea Wi FS), and the Advanced Land Imager(ALI) on board three satellites, respectively. The mean difference between MODIS and Sea Wi FS is less than 3.14%, and the difference between MODIS and ALI is less than 4.75%. These results indicate that the proposed cross-calibration method not only compensates for the RSR mismatches but also reduces the differences in lunar observation geometry. Thus,radiance calibration of any satellite instrument can be validated with a reference instrument bridged by the moon.

关键词： radiometric cross-calibration multiple sensors calibration reference

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Co-culture of Schwann cells and endothelial cells for synergistically regulating dorsal root ganglion behavior on chitosan-based anisotropic topology for peripheral nerve regeneration

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Burns & Trauma 2022年第1期10卷 340-356页

作者： Tiantian Zheng Linliang wu Shaolan Sun Jiawei Xu Qi Han Yifan Liu ronghua wu Guicai Li Key laboratory of Neuroregeneration of Jiangsu and Ministry of Education Co-innovation Center of Neuroregenera-tionNMPA Key Lab for Research and Evaluation of Tissue Engineering Technology ProductsNantong University.226001NantongP.R.China School of Medicine Nantong University.226001NantongP.R.China Guangxi Key Laboratory of Regenerative Medicine Guangxi Medical University530021NanningP.R.China National Engineering Laboratory for Modern Silk Soochow UniversitySuzhou 215123China

Background:Anisotropic topologies are known to regulate cell-oriented growth and induce cell differentiation,which is conducive to accelerating nerve regeneration,while co-culture of endothelial cells(ECs)and Schwann cells(SCs)can significantly promote the axon growth of dorsal root ganglion(DRG).However,the synergistic regulation of EC and SC co-culture of DRG behavior on anisotropic topologies is still rarely *** study aims to investigate the effect of anisotropic topology co-cultured with Schwann cells and endothelial cells on dorsal root ganglion behavior for promoting peripheral nerve ***:Chitosan/artemisia sphaerocephala(CS/AS)scaffolds with anisotropic topology were first prepared using micro-molding technology,and then the surface was modified with dopamine to facilitate cell adhesion and *** physical and chemical properties of the scaffolds were characterized through morphology,wettability,surface roughness and component *** and ECs were co-cultured with DRG cells on anisotropic topology scaffolds to evaluate the axon growth ***:Dopamine-modified topological CS/AS scaffolds had good hydrophilicity and provided an appropriate environment for cell *** immunofluorescence showed that in contrast to DRG growth alone,co-culture of SCs and ECs could not only promote the growth of DRG axons,but also offered a stronger guidance for orientation growth of neurons,which could effectively prevent axons from tangling and knotting,and thus may significantly inhibit neurofibroma ***,the co-culture of SCs and ECs could promote the release of nerve growth factor and vascular endothelial growth factor,and up-regulate genes relevant to cell proliferation,myelination and skeletal development via the PI3K-Akt,MAPK and cytokine and receptor chemokine ***:The co-culture of SCs and ECs significantly improved the growth behavior of DRG on anisotropic topological scaffolds,which may pr

关键词： Schwann cells Endothelial cells Dorsal root ganglion behavior Co-culture Anisotropic topology Regulation mechanism Nerve Regeneration

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Ploidy and fruit trait variation in oil-tea Camellia:Implications for ploidy breeding

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Journal of Integrative Agriculture 2024年第8期23卷 2662-2673页

作者： Yanmin Li Liangjing Yin Xianyu He Cenlong Hu ronghua wu Qian Long Shixin Xiao Deyi Yuan Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education/Key Laboratory of Non-Wood Forest Products of State Forestry Administration Central South University of Forestry and TechnologyChangsha 410004China Qiandongnan Institute of Forestry Qiandongnan Miao and Dong Autonomous Prefecture 556000China Dongfeng Forest Farm of Liping County Qiandongnan Miao and Dong Autonomous Prefecture 557300China

Plant polyploidy often occurs in conjunction with higher yield and superior ***,obtaining polyploid germplasms is a significant part of *** oil-tea Camellia tree is an important native woody plant that produces high-quality edible oil and includes many species of Camellia with different ***,whether higher ploidy levels in oil-tea Camellia trees are related to better traits remains *** this study,the ploidy levels of 30 different oil-tea Camellia strains in three different species in the *** were determined by flow cytometry and chromosome preparation,and the phenotypic characteristics and fatty acid compositions of the fruits were examined by field observations and laboratory *** correlations between the ploidy level of oil-tea Camellia and the main traits of the fruit were *** results showed that 10 Camellia lanceoleosa strains were diploid,10 Camellia meiocarpa strains were tetraploid and 10 Camellia oleifera strains were *** *** had larger fruit size and weight,more seeds per fruit,greater seed weight per fruit,higher oil content and greater yield per crown width than tetraploid *** and diploid ***,but their fruit peel thickness and fresh seed rate were significantly lower,and these traits were significantly correlated with ploidy *** addition,in terms of fatty acid composition,hexaploid *** had a higher oleic acid content than tetraploid *** and diploid ***,but their linoleic acid,linolenic acid and arachidonic acid contents were *** contents of palmitic acid,stearic acid and total unsaturated fatty acids were not significantly correlated with ploidy *** conclusion,certain correlations exist between the main characteristics of oil-tea Camellia fruit and the ploidy level,and increasing the ploidy level led to an increase in fruit yield with no effect on oil *** discovery of variations in the m

关键词： oil-tea Camellia ploidy level fruit traits correlation polyploidization

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The Development and Current Situation of Medium and Large Buses in China

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中国汽车（英文版） 2002年第7期 7-8页

作者： wu ronghua

1 A Brief introduction Production of medium and large buses in China started in the early 1950’s with manufacturing plants mainly in Shanghai, Beijing and similar cities. The 1990s witnessed a rapid development of me... 详细信息

关键词： witnessed Medium sized Zhengzhou thousand venture sector engaged becoming maintained

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Current Situation Of Chinese Large - Medium Bus Industry

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中国汽车（英文版） 2001年第4期 13-16页

作者： By wu ronghua

With the development of the reform and open policy and the economic advance, Chinese largemedium bus industry has obtained rare opportunity and encouraging progress. The rapid development of road construction, urban construction and tourism has brought great demand and drive to the bus market. Almost all the world-famous large medium bus corporations have found their own partners in China, organizing joint ventures and capturing the share of Chinese large - medium bus market. In the domestic market, you could find an epitome embodying the competing posture of global large and medium

关键词：二二三

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Status Quo and Prospects of Heavy Vehicles in China

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中国汽车（英文版） 1998年第3期 6-10页

作者： wu ronghua

1. History The vehicles with load capacity over 8 tons are classified as heavy vehicles according to China auto industry standards. The first 8t track, JN150, was made in 1960 by Jinan Auto Works. This COE, 4×2 truck... 详细信息

关键词： Status Quo and Prospects of Heavy Vehicles in China Star

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