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Tetrapyrrole biosynthesis pathway regulates plastid-to-nucleus signaling by controlling plastid gene expression in plants

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Plant Communications 2023年第1期4卷 1-12页

作者： Yunlong Wang Yihua Wang Xiaopin Zhu Yulong Ren Hui Dong Erchao Duan Xuan Teng Huanhuan Zhao rongbo chen Xiaoli chen Jie Lei Hang Yang Yunlu Tian Liangming chen Xi Liu Shijia Liu Ling Jiang Haiyang Wang Jianmin Wan State Key Laboratory for Crop Genetics and Germplasm Enhancement Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjing 210095P.R.China National Key Facility for Crop Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081P.R.China

Plastid-to-nucleus retrograde signaling coordinates nuclear gene expression with chloroplast developmental status and is essential for the photoautotrophic lifestyle of *** studies have established that tetrapyrrole biosynthesis(TPB)and plastid gene expression(PGE)play essential roles in plastid retrograde signaling during early chloroplast biogenesis;however,their functional relationship remains *** this study,we generated a series of rice TPB-related gun(genome uncoupled)mutants and systematically analyzed their effects on nuclear and plastid gene expression under normal conditions or when subjected to treatments with norflurazon(NF;a noncompetitive inhibitor of carotenoid biosynthesis)and/or lincomycin(Lin;a specific inhibitor of plastid translation).We show that under NF treatment,expression of plastid-encoded polymerase(PEP)-transcribed genes is significantly reduced in the wild type but is derepressed in the TPB-related gun *** further demonstrate that the derepressed expression of PEPtranscribed genes may be caused by increased expression of the PEP core subunit and nuclear-encoded sigma factors and by elevated copy numbers of plastid genome per haploid *** addition,we show that expression of photosynthesis-associated nuclear genes(PhANGs)and PEP-transcribed genes is correlated in the rice TPB-related gun mutants,with or without NF or Lin treatment.A similar correlation between PhANGs and PGE is also observed in the Arabidopsis gun4 and gun5 ***,we show that increased expression of PEP-transcribed plastid genes is necessary for the gun phenotype in NF-treated TPB-related gun ***,we provide evidence that these TPB-related GUN genes act upstream of GUN1 in the regulation of retrograde *** together,our results suggest that the TPB-related GUN genes control retrograde plastid signaling by regulating the PGE-dependent retrograde signaling pathway.

关键词： tetrapyrrole biosynthesis plastid gene expression plastid retrograde signal

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城市地铁隧道断面检测系统研究与应用

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测绘地理信息 2017年第1期42卷 115-118页

作者：陈荣波广东有色工程勘察设计院广东广州510080

针对城市地铁隧道断面检测工作的特点和问题,结合生产单位的实际需求,采用移动端蓝牙通讯和数据库等技术,设计并实现了城市地铁隧道断面检测原型系统,解决了城市地铁隧道断面检测数据处理不及时、可视化程度低等一系列问题,提高了断面... 详细信息

针对城市地铁隧道断面检测工作的特点和问题,结合生产单位的实际需求,采用移动端蓝牙通讯和数据库等技术,设计并实现了城市地铁隧道断面检测原型系统,解决了城市地铁隧道断面检测数据处理不及时、可视化程度低等一系列问题,提高了断面检测工作的效率。

关键词：地铁隧道断面检测系统设计数据管理

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Rice FLOURY ENDOSPERM22, encoding a pentatricopeptide repeat protein, is involved in both mitochondrial RNA splicing and editing and is crucial for endosperm development

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Journal of Integrative Plant Biology 2023年第3期65卷 755-771页

作者： Hang Yang Yunlong Wang Yunlu Tian Xuan Teng Zehui Lv Jie Lei Erchao Duan Hui Dong Xue Yang Yuanyan Zhang Yinglun Sun Xiaoli chen Xiuhao Bao rongbo chen Chuanwei Gu Yipeng Zhang Xiaokang Jiang Wenyu Ma Pengcheng Zhang Yi Ji Yu Zhang Yihua Wang Jianmin Wan State Key Laboratory for Crop Genetics and Germplasm Enhancement Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjing 210095China National Key Facility for Crop Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Most of the reported P-type pentatricopeptide repeat(PPR) proteins play roles in organelle RNA stabilization and splicing. However, P-type PPRs involved in both RNA splicing and editing have rarely been reported, and their underlying mechanism remains largely unknown. Here, we report a rice floury endosperm22(flo22) mutant with delayed amyloplast development in endosperm cells. Map-based cloning and complementation tests demonstrated that FLO22 encodes a mitochondrion-localized P-type PPR *** of FLO22 resulting in defective transsplicing of mitochondrial nad1 intron 1 and perhaps causing instability of mature transcripts affected assembly and activity of complex Ⅰ, and mitochondrial morphology and function. RNA-seq analysis showed that expression levels of many genes involved in starch and sucrose metabolism were significantly down-regulated in the flo22mutant compared with the wild type, whereas genes related to oxidative phosphorylation and the tricarboxylic acid cycle were significantly upregulated. In addition to involvement in splicing as a P-type PPR protein, we found that FLO22 interacted with DYW3, a DYW-type PPR protein, and they may function synergistically in mitochondrial RNA editing. The present work indicated that FLO22 plays an important role in endosperm development and plant growth by participating in nad1 maturation and multi-site editing of mitochondrial messager RNA.

关键词： endosperm development Oryza sativa PPR proteins RNA editing RNA splicing

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含水层压密引起其特征参数变化的实验

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吉林大学学报（地球科学版） 2013年第6期43卷 1958-1965页

作者：陈荣波束龙仓鲁程鹏李伟河海大学水文水资源与水利工程科学国家重点实验室南京210098 中国水电顾问集团昆明勘测设计研究院昆明650051 江苏省地质调查研究院南京210018

水文地质参数在含水层压密过程中不断变化，这是诸多地面沉降模型实际应用中的难点之一。就含水层压密对水文地质参数的影响开展了初步的实验，结果表明，压密前后含水层的渗透系数和给水度发生了较大的变化。其中：渗透系数随模拟含水... 详细信息

水文地质参数在含水层压密过程中不断变化，这是诸多地面沉降模型实际应用中的难点之一。就含水层压密对水文地质参数的影响开展了初步的实验，结果表明，压密前后含水层的渗透系数和给水度发生了较大的变化。其中：渗透系数随模拟含水层介质压密过程的变化几乎呈线性的减小趋势，线性拟合决定系数为0．9592～0．9971；给水度随着含水层的压实而表现出下降的趋势较为复杂，随介质类型的不同而有差异。渗透系数和给水度的下降速度在不同介质中也有一定的差异。在本研究的实验模型尺度下，给水度和渗透系数呈现一定的线性正相关关系，一次线性相关函数斜率为0．0013～0．0051，截距为0．0116～0．0381，基本相近且在相同数量级。

关键词：地面沉降含水层压密渗透系数给水度砂柱试验

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我国高校农业科技成果转化制约因素分析

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农业网络信息 2016年第5期 94-96页

作者：陈荣博湖南省高警局湖南长沙410100

本文通过对高校现有农业科技成果转化情况进行调查的基础上,总结了高校农业科技成果转化过程中高校本身的障碍、农业科技体制机制不够完善及外部环境三个方面的制约因素,并据此提出了强化高校自身主体地位,改善农业科技体制、推广体制... 详细信息

本文通过对高校现有农业科技成果转化情况进行调查的基础上,总结了高校农业科技成果转化过程中高校本身的障碍、农业科技体制机制不够完善及外部环境三个方面的制约因素,并据此提出了强化高校自身主体地位,改善农业科技体制、推广体制和改善农业科技成果转化外部环境三个方面的对策。以促进我国高校农业科技成果转化为社会生产力,提升科技成果转化率,更好的为我国农业发展做出贡献。

关键词：高校农业科技成果转化

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Low Temperature Synthesis and Optical Properties of ZnO Nanowires

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Journal of Semiconductors 2007年第10期28卷 1503-1507页

作者：常鹏刘肃陈溶波唐莹韩根亮兰州大学物理科学与技术学院兰州730000 甘肃省科学院传感技术研究所兰州730000

ZnO nanowire arrays are fabricated on anodized aluminum oxide templates with electric field-assisted electrochemical technology. Transmission electron microscopy results indicate that the nanowires are straight and uniform. X-ray diffraction patterns indicate that the nanowires are highly oriented. The result of selected area electron diffraction suggests that the nanowires are single crystals. The photoluminescence spectrum presents a broad-band luminescence in the region of 350-650nm. The effect of the assisted transverse electric field on the growth process of ZnO nanowires is also discussed.

关键词： ZnO electrochemical nanowires PL spectrum

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腹腔镜手术治疗老年急性结石性胆囊炎的临床疗效分析

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系统医学 2024年第16期9卷 105-108页

作者：梁俊陈荣波陈俊泰州市第三人民医院普外科江苏泰州225300

目的分析腹腔镜治疗老年急性结石性胆囊炎的疗效。方法目的选取2022年1月—2023年12月泰州市第三人民医院收治的老年急性结石性胆囊炎患者76例,以治疗方法不同分为观察组与对照组,各38例,分别以腹腔镜和传统开腹治疗,对比两组临床疗... 详细信息

目的分析腹腔镜治疗老年急性结石性胆囊炎的疗效。方法目的选取2022年1月—2023年12月泰州市第三人民医院收治的老年急性结石性胆囊炎患者76例,以治疗方法不同分为观察组与对照组,各38例,分别以腹腔镜和传统开腹治疗,对比两组临床疗效、手术相关指标、术后并发症发生率。结果观察组临床疗效高于对照组,差异有统计学意义(P<0.05);观察组手术用时、术中出血量、术后排气时间、总住院时间分别(44.23±4.85)min、(24.17±4.01)mL、(17.44±2.68)h、(6.24±1.10)d,优于对照组的(52.15±5.31)min、(30.94±5.26)mL、(22.17±3.15)h、(8.03±1.42)d,差异有统计学意义(t=6.788、6.309、7.050、6.143,P均<0.05);观察组术后并发症低于对照组,差异有统计学意义(P<0.05)。结论老年急性结石性胆囊炎以腹腔镜治疗,有效率高,并发症发生率低。

关键词：腹腔镜微创手术结石性胆囊炎临床疗效

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