检索结果-南通市图书馆

Dynamic and Coordinated Expression Changes of Rice Small RNAs in Response to Xanthomonas oryzae ***

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2015年第11期42卷 625-637页

作者： Ying-Tao Zhao Meng Wang Zhi-Min Wang rong-xiang fang Xiu-Jie Wang Yan-Tao Jia State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyChinese Academy of Sciences University of Chinese Academy of Sciences State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of Sciences

Endogenous small RNAs are newly identified players in plant immune responses, yet their roles in rice （Oryza sativa） responding to pathogens are still less understood, especially for pathogens that can cause severe yield losses. We examined the small RNA expression profiles of rice leaves at 2, 6, 12, and 24 hours post infection ofXanthomonas oryzae pv. oryzae （Xoo） virulent strain PXO99, the causal agent of rice bacterial blight disease. Dynamic expression changes of some miRNAs and trans-acting siRNAs were identified, together with a few novel miRNA targets, including an RLK gene targeted by osa-miR159a. 1. Coordinated expression changes were observed among some small RNAs in response to Xoo infection, with small RNAs exhibiting the same expression pattern tended to regulate genes in the same or related signaling pathways, including auxin and GA signaling pathways, nutrition and defense-related pathways. These findings reveal the dynamic and complex roles of small RNAs in rice-Xoo interactions, and identify new targets for regulating plant responses to Xoo.

关键词： Small RNA Rice bacterial blight disease osa-miR159

Overexpression of an F-box Protein Gene Reduces Abiotic Stress Tolerance and Promotes Root Growth in Rice

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2011年第1期4卷 190-197页

作者： Yong-Sheng Yan Xiao-Ying Chen Kun Yang Zong-Xiu Sun Ya-Ping Fu Yu-Man Zhang rong-xiang fang State Key Laboratory of Plant Genomics Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China National Plant Gene Research Center Beijing 100101 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China Present address: School of Life Science and Technology University of Electronic Science and Technology of China Chengdu 610054 China State Key Laboratory of Rice Biology National Rice Research Institute Hangzhou 310006 China

As one of the largest gene families, F-box domain proteins have important roles in regulating various devel- opmental processes and stress responses. In this study, we have investigated a rice F-box domain gene, MAIF1. The MAIF1 protein is mainly localized in the plasma membrane and nucleus. MAIF1 expression is induced rapidly and strongly by abscisic acid （ABA） and abiotic stresses. MAIF1 expression is also induced in root tips by sucrose, independent of its hy- drolytic hexose products, glucose and fructose, and the plant hormones auxin and cytokinin. Overexpression of MAIF1 reduces rice ABA sensitivity and abiotic stress tolerance and promotes rice root growth. These results suggest that MAIF1 is involved in multiple signaling pathways in regulating root growth. Growth restraint in plants is an acclimatization strategy against abiotic stress, Our results also suggest that MAIF1 plays the negative role in response to abiotic stress possibly by regulating root growth.

关键词： Rice (Oryza sativa L.) F-box domain abscisic acid abiotic stress tolerance root growth.

Laodelphax striatellus Atg8 facilitates Rice stripe virus infection in an autophagy-independent manner

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2021年第2期28卷 315-329页

作者： Yuan-Ling Yu Meng-Ting Zhang Yan Huo Ji-Liang Tang Qing Liu Xiao-Ying Chen rong-xiang fang Li-Li Zhang State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of ScienceBeijingChina CAS Center for Excellence in Biotic Interactions University of the Chinese Academy of SciencesBeijingChina State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Guangxi UniversityNanningChina National Plant Gene Research Center BeijingChina

Rice stripe virus(RSV)is the causative agent of rice stripe disease and is completely dependent on insect vectors for its plant-to-plant *** striatellus is the major insect vector for *** this study,we explored the interactions be-tween RSV infection and *** autophagy,a potential intrinsic antiviral mechanism in *** found that *** autophagic activity did not affect RSV infection;however,the autophagy related-8(Atg8)gene significantly enhanced virus ***-ing RSV initial infection within the *** midgut,silencing of Atg8 expression significantly decreased the phosphorylation of c-Jun N-terminal kinase(p-JNK);however,when RSV infection is absent,silencing of Atg8 did not alter p-JNK *** results indicated that Atg8 might activate the JNK machinery by allowing more virus infection into *** further revealed that Atg8-deficiency significantly decreased RSV accumu-lation on the surface of the insect midgut epithelial cells,suggesting a receptor trafficking function of the y-aminobutyric acid receptor-associated protein *** the RSV ovary entry as a model,in which vitellogenin receptor(V gR)mediates RSV cell entry,we clarified that Atg8-deficiency decreased the abundance of V gR localizing on the cytomem-brane and disturbed the attachment of RSV in the germarium ***,these results revealed an autophagy-independent function of *** Atg8 that enhances RSV initial infection by increasing virus attachment on the infection sites.

关键词： Atg8 insect autophagy Laodelphax striatellus receptor trafficking Rice stripe virus viral initial infection

A Novel Rice Gene, NRR Responds to Macronutrient Deficiency and Regulates Root Growth

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2012年第1期5卷 63-72页

作者： Yu-Man Zhang Yong-Sheng Yan Li-Na Wang Kun Yang Na Xiao Yun-Feng Liu Ya-Ping Fu Zong-Xiu Sun rong-xiang fang Xiao-Ying Chen State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China National Plant Gene Research Center Beijing 100101China Graduate School of the Chinese Academy of Sciences Beijing 100039China Department of Ornamental Horticulture and Landscape Architecture China Agricultural UniversityBeijing 100193China Current address:School of Life Science and Technology University of Electronic Science and Technology of ChinaChengdu 610054China State Key Laboratory of Rice Biology National Rice Research InstituteHangzhou 310006 China

To better understand the response of rice to nutrient stress, we have taken a systematic approach to identify rice genes that respond to deficiency of macronutrients and affect rice growth. We report here the expression and bio- logical functions of a previously uncharacterized rice gene that we have named NRR （nutrition response and root growth）. NRR is alternatively spliced, producing two 5＇-coterminal transcripts, NRRa and NRRb, encoding two proteins of 308 and 223 aa, respectively. Compared to NRRb, NRRa possesses an additional CCT domain at the C-terminus. Expression of NRR in rice seedling roots was significantly influenced by deficiency of macronutrients. Knock-down of expression of NRRa or NRRb by RNA interference resulted in enhanced rice root growth. By contrast, overexpression of NRRa in rice exhibited significantly retarded root growth. These results revealed that both NRRa and NRRb played negative regulatory roles in rice root growth. Our findings suggest that NRRa and NRRb, acting as the key components, modulate the rice root archi- tecture with the availability of macronutrients.

关键词： Nutrient stress alternative splicing root growth rice.

Efficient and simple synthesis of B_(2)O_(3)-Al_(2)O_(3)nanocomposites assisted by deep eutectic solvent for production of 5-hydroxymethylfurfural from glucose

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2025年第1期18卷 236-248页

作者： Yuchen Wang Kai rong Shanlei Chang Youxing fang Shaojun Dong State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei 230026China College of Chemistry Jilin UniversityChangchun 130012China

The catalytic conversion of glucose to high valueadded platform chemical 5-hydroxymethylfurfural(HMF)is a promising biorefinery process,and alumina-boria catalysts are considered to be green and mild solid acid catalysts for this catalytic ***,compared to the common synthesis methods with complicated steps,we reported a simple and efficient strategy to prepare B_(2)O_(3)-Al_(2)O_(3)nanocomposites by calcining cost-effective glucose-urea deep eutectic solvent(DES)solution containing the *** prepared B_(2)O_(3)-Al_(2)O_(3)nanocomposites exhibited an open three-dimensional skeleton and two-dimensional porous lamellar substructure,endowing them with a high specific surface area(228.27 m2/g).The introduction of boron changed the ratios of different aluminum species(AlⅣ,AlⅤ,and AlⅥ)and borate species(BO3 and BO4),thus further affecting the acidity and the types of acid sites of the *** prepared B_(2)O_(3)-Al_(2)O_(3)bifunctional acid catalysts possessing abundant Lewis acid sites and adjustable Brønsted acid sites showed complete glucose conversion and 55.38%of HMF yield under the optimum *** study proposed a concise method to synthesize alumina-boria solid acid catalysts assisted by glucose-urea *** hope to extend the application and prospect of this efficient and simple synthesis strategy.

关键词： 5-hydroxymethylfurfural(HMF) deep eutectic solvent glucose alumina-boria acid catalyst

Inhibiting ceramide synthase 5 expression in microglia decreases neuroinflammation after spinal cord injury

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第10期20卷 2955-2968页

作者： Wei Zhang Yubao Lu Ruoqi Shen Yingjie Wu Chenrui Liu Xingxing fang Liangming Zhang Bin Liu Limin rong Department of Spine Surgery The Third Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina National Medical Products Administration(NMPA)Key Laboratory for Quality Research and Evaluation of Cell Products GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Quality Control of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Engineering and Technology Research of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina

Microglia,the resident monocyte of the central nervous system,play a crucial role in the response to spinal cord ***,the precise mechanism remains *** investigate the molecular mechanisms by which microglia regulate the neuroinflammatory response to spinal cord injury,we performed single-cell RNA sequencing dataset analysis,focusing on changes in microglial *** found that the MG1 subpopulation emerged in the acute/subacute phase of spinal cord injury and expressed genes related to cell pyroptosis,sphingomyelin metabolism,and neuroinflammation at high ***,we established a mouse model of contusive injury and performed intrathecal injection of siRNA and molecular inhibitors to validate the role of ceramide synthase 5 in the neuroinflammatory responses and pyroptosis after spinal cord ***,we established a PC12-BV2 cell co-culture system and found that ceramide synthase 5 and pyroptosis-associated proteins were highly expressed to induce the apoptosis of neuron *** ceramide synthase 5 expression in a mouse model of spinal cord injury effectively reduced ***,ceramide synthase 5-induced pyroptosis was dependent on activation of the NLRP3 signaling *** ceramide synthase 5 expression in microglia in vivo reduced neuronal apoptosis and promoted recovery of neurological ***2g7 formed a“bridge”between sphingolipid metabolism and ceramide synthase 5-mediated cell death by inhibiting the NLRP3 signaling ***,these findings suggest that inhibiting ceramide synthase 5 expression in microglia after spinal cord injury effectively suppressed microglial pyroptosis mediated by NLRP3,thereby exerting neuroprotective effects.

关键词： ceramide synthase 5 gasdermin D microglia neuroinflammation NLRP3 nuclear factor kappa B Pla2g7 pyroptosis sphingomyelin metabolism spinal cord injury

在线全文

学校读者我要写书评

暂无评论

广东硇洲岛的瓣鳃类

四川动物 2002年第4期21卷 232-235页

作者：肖汉洪陈向荣李方满肇庆学院生物系广东肇庆526061

根据1999～2001年在广东硇洲岛海滨的岩石岸、砾石滩、泥沙滩、沙滩、红树林区潮间带和近海区潮下带等各种生态环境采集到的瓣鳃类动物的整理鉴定,获得瓣鳃类77种,分隶于24科44属。本文对其区系组成及分布特点进行了详细的论述。

关键词：广东硇洲岛瓣鳃类潮间带潮下带区系贝类

CONVERSION RATES OF SURFACE HOX RADICALS IN BEIJING CITY

在线全文

学校读者我要写书评

暂无评论

Chinese Geographical Science 2004年第1期14卷 34-38页

作者： RENXin-rong WANGLi-xin WANGHui-xiang MIAOGuo-fang StateKeyLaboratoryofEnvironmentalSimulationandPollutionControl CollegeofEnvironmentalSciencesPekingUniversityBeijing100871P.R.China

Surface OH radical concentration in Beijing City was measured by impregnated filter trapping technique-high performance liquid chromatography (IFT-HPLC). The observed concentration of OH radical showed obvious diurnal and seasonal variations, with maximum readings at noon or afternoon, similar to 80x10(6)OH/cm(3) in summer and similar to 20x10(6)-40x10(6)OH/cm(3) in fall. On the basis of measured data, the reaction rates related to the photochemical process of HO(x) (OH+HO(2)) were derived and characteristics of atmospheric chemical processes in the city were analyzed. The results showed that conversion rates of atmospheric OH and HO(2) in the summer of Beijing City were about 700x10(6) molecule/(cm(3) . s) and 600x10(6)molecule/(cm(3) . s), respectively. And the net production of OH in the air of the city mainly originated from the photolysis of the gaseous HNO(2), and the main sink of OH were the photochemical reactions with VOCs, NO(2), HCHO and CO. It was different from the clean area.

关键词： surface HO(x) radical conversion rate atmospheric photochemistry city atmospheric environment

妊高征患者血清对内皮细胞分泌NO与ET-1的影响

在线全文

学校读者我要写书评

暂无评论

实用妇产科杂志 2001年第1期17卷 31-33页

作者：王晨虹郑湘榕方建珍谈海英萧根秀湖南医科大学附属第二医院湖南长沙410011

目的 :探讨妊高征患者血清中是否存在引起内皮细胞功能改变的因素以及与妊高征发生的关系。方法 :无菌收集妊高征及正常孕妇产前静脉血制备血清。传代培养人脐静脉内皮细胞 (HUVEC) ,当其融合为单层时 ,分别加入收集的产前血清 ,置 37℃... 详细信息

目的 :探讨妊高征患者血清中是否存在引起内皮细胞功能改变的因素以及与妊高征发生的关系。方法 :无菌收集妊高征及正常孕妇产前静脉血制备血清。传代培养人脐静脉内皮细胞 (HUVEC) ,当其融合为单层时 ,分别加入收集的产前血清 ,置 37℃ ,5 % CO2 培养箱中孵育 2 4h,收集培养液 ,采用 Griess法和放免法测定 NO及 ET- 1含量变化。结果 :1中、重度妊高征组 HU VEC培养液中 NO量明显降低 ,而轻度妊高征与正常妊高征组无差异。 2中、重度妊高征HUVEC培养液中 ET- 1量明显增高 ,而轻度妊高征与正常妊高征组无差异。结论 :中、重度妊高征患者产前血清可能含有某些因素 ,可引起血管内皮细胞功能改变 ,使 NO、ET- 1的分泌失衡。

关键词：妊娠高血压综合征内皮细胞一氧化氮内皮素