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Microfluidic thermotaxic selection of highlymotile sperm and in vitro fertilization

Bio-Design and Manufacturing 2024年第5期7卷 687-700页

作者： Sihan Chen Jiemin Chen Zihan qin Jibo Wang Yuwen Wang rong Liu Wen Zhao Ming Zhang Yuanzhen Zhang Mengcheng Luo Pu Chen Tissue Engineering and Organ Manufacturing(TEOM)Lab Department of Biomedical EngineeringTaiKang Medical School(School of Basic Medical Sciences)Wuhan UniversityWuhan 430071China Hubei Provincial Key Laboratory of Developmentally Originated Disease TaiKangMedical School(School of Basic Medical Sciences)Wuhan UniversityWuhan 430071China Reproductive Medicine Center Zhongnan Hospital of Wuhan UniversityWuhan 430071China TaiKang Center for Life and Medical Sciences Wuhan UniversityWuhan 430071China

The selection of the most motile and functionally competent sperm is an essential basis for in vitro fertilization(IVF)and normal embryonic *** adopted clinical approaches for sperm sample processing intensely rely on centrifugation and wash steps that may induce mechanical damage and oxidative stress to *** a few microfluidic sperm sorting devices may avoid these adverse effects by exploiting intrinsic guidance mechanisms of sperm swimming,none of these approaches have been fully validated by clinical-grade assessment *** this study,a microfluidic sperm sorting device that enables the selection of highly motile and functional sperm via their intrinsic thermotaxis is *** by the temperature microenvironment in the fallopian tube during natural sperm selection,a microfluidic device with controllable temperature gradients along the sperm separation channel was designed and *** study investigated the optimal temperature conditions for human sperm selection and fully characterized thermotaxis-selected sperm with 45 human sperm *** indicated that a temperature range of 35–36.5℃along the separation channel significantly improves human sperm motility rate((85.25±6.28)%vs.(60.72±1.37)%;P=0.0484),increases normal sperm morphology rate((16.42±1.43)%vs.(12.55±0.88)%;P<0.0001),and reduces DNA fragmentation((7.44±0.79)%vs.(10.36±0.72)%;P=0.0485)compared to the nonthermotaxis *** thermotaxis is species-specific,and selected mouse sperm displayed the highest motility in response to a temperature range of 36–37.5℃ along the separation ***,IVF experiments indicated that the selected sperm permitted an increased fertilization rate and improved embryonic development from zygote to *** microfluidic thermotaxic selection approach will be translated into clinical practice to improve the IVF success rate for patients with oligozoospermia and asthenozoospermia.

关键词： Microfluidic device Thermotaxis Sperm sorting Assisted reproductive technology In vitro fertilization

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新中国成立70年来广播“自己走路”的理论意蕴、变迁脉络与特征研析

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编辑之友 2019年第9期 84-92,101页

作者：覃信刚覃榕云南师范大学传媒学院云南昆明650118 暨南大学新闻与传播学院广东广州510000

“广播要学会自己走路”这一论述已提出近70年。70年来,新中国广播“自己走路”,从小到大,由少到多,波澜壮阔,取得了举世瞩目的成就。文章对新中国广播“自己走路”论述的由来、认知发展进行了分析,对变迁脉络做出了历史性梳理,勾勒出... 详细信息

“广播要学会自己走路”这一论述已提出近70年。70年来,新中国广播“自己走路”,从小到大,由少到多,波澜壮阔,取得了举世瞩目的成就。文章对新中国广播“自己走路”论述的由来、认知发展进行了分析,对变迁脉络做出了历史性梳理,勾勒出广播管理体制优化、节目模式进化、覆盖范围演化等路线图,进而研析出广播“自己走路”的变迁始终与党的中心任务变化紧密相连,始终践行全心全意为人民服务的宗旨,坚持以人民为中心的导向等主要特征。

关键词：新中国成立70周年广播“自己走路” 理论意蕴变迁脉络特征研析

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Oral and ocular transmission of severe fever with thrombocytopenia syndrome virus

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Infectious Medicine 2022年第1期1卷 2-6页

作者： Chuan-Min Zhou Rui Qi Xiang-rong qin Li-Zhu Fang Hui-Ju Han Xiao-Ying Lei Xue-Jie Yu State Key Laboratory of Virology School of Public HealthWuhan UniversityWuhanChina School of Public Health Lanzhou UniversityLanzhouGansu ProvinceChina Department of Clinical Laboratory the Second Hospital of Shandong UniversityJinanShandong ProvinceChina Department of Microbiological Laboratory Technology School of Public HealthCheeloo College of MedicineShandong UniversityJinanShandongChina

Background:Severe fever with thrombocytopenia syndrome virus(SFTSV)is a tick-borne bunyavirus that could cause a severe hemorrhagic fever termed SFTS with a high fatality rate of up to 30%.Importantly,SFTSV is frequently transmitted from person-to-person and patients’blood or excreta are considered as the risk factors for transmission of ***,the mechanism of person-to-person transmission of SFTSV is still ***:In this study,wild-type(WT)C57BL/6 J mice and a lethal SFTSV mouse model IFNAR−/−A129 mice were utilized to evaluate whether SFTSV could be transmitted via oral or ocular routes.C57BL/6 J mice were inoculated with cell-cultured SFTSV via oral and ocular ***−/−A129 mice were inoculated with cell-cultured SFTSV or SFTSV infected mouse acute sera via oral and ocular ***:We found that SFTSV antibody positive rates in C57BL/6 J mice were 70%(7/10)and 30%(3/10)in the oral inoculation group and ocular inoculation group,respectively on day 21 post SFTSV *** mortality rates of IFNAR−/−mice with oral and ocular inoculation of cell-cultured SFTSV were 100%and 83.33%(5/6),respectively on day 6 post *** mortality rates of IFNAR−/−mice with oral and ocular inoculation of SFTSV infected mouse acute serum were 100%and 66.67%(4/6),respectively on day 9 post ***:Together,our results show that SFTSV can be transmitted effectively through oral and ocular mem-brane,suggesting exposure to SFTS positive excreta may be a high-risk factor of nosocomial transmission of SFTSV in hospitals and/or *** members and healthcare workers should be protected properly during taking care of SFTS patients to prevent SFTSV nosocomial infection.

关键词： SFTSV Nosocomial infection Person-to-person transmission Fecal-oral Fecal-ocular

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Rapid development of double-hit mRNA antibody cocktail against orthopoxviruses

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Signal Transduction and Targeted Therapy 2024年第4期9卷 1768-1776页

作者： Hang Chi Suo-Qun Zhao Ru-Yi Chen Xing-Xing Suo rong-rong Zhang Wen-Hui Yang Dong-Sheng Zhou Min Fang Bo Ying Yong-Qiang Deng Cheng-Feng qin State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyAMMS100071BeijingChina College of Life Sciences Inner Mongolia Agricultural UniversityHohhot010018Inner MongoliaChina School of Life Sciences Henan UniversityKaifeng475004HenanChina Suzhou Abogen Biosciences Co. LtdSuzhou215123JiangsuChina Research Unit of Discovery and Tracing of Natural Focus Diseases Chinese Academy of Medical Sciences100071BeijingChina

The Orthopoxvirus genus,especially variola virus(VARV),monkeypox virus(MPXV),remains a significant public health threat *** development of therapeutic antibodies against orthopoxviruses is largely hampered by the high cost of antibody engineering and manufacturing ***-encoded antibodies have emerged as a powerful and universal platform for rapid antibody ***,by using the established lipid nanoparticle(LNP)-encapsulated mRNA platform,we constructed four mRNA combinations that encode monoclonal antibodies with broad neutralization activities against *** vivo characterization demonstrated that a single intravenous injection of each LNP-encapsulated mRNA antibody in mice resulted in the rapid production of neutralizing *** importantly,mRNA antibody treatments showed significant protection from weight loss and mortality in the vaccinia virus(VACV)lethal challenge mouse model,and a unique mRNA antibody cocktail,Mix2a,exhibited superior in vivo protection by targeting both intracellular mature virus(IMV)-form and extracellular enveloped virus(EEV)-form *** summary,our results demonstrate the proof-of-concept production of orthopoxvirus antibodies via the LNP-mRNA platform,highlighting the great potential of tailored mRNA antibody combinations as a universal strategy to combat orthopoxvirus as well as otheremerging viruses.

关键词： proof universal neutral

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Effects of Bisphenol A and Its Substitute, Bisphenol F, on the Gut Microbiota in Mice

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Biomedical and Environmental Sciences 2024年第1期37卷 19-30页

作者： MENG Li Ying TAO Wen Fu LI Jing ZHU Min ZHONG De Bin ZHOU Jing qin Xue WEI rong Guo Department of Clinical Laboratory The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021GuangxiChina Department of Clinical Laboratory The Fifth Affiliated Hospital of Guangxi Medical UniversityNanning 530021GuangxiChina Jiangsu Key Laboratory of Environmental Engineering Jiangsu Academy of Environmental SciencesNanjing 210000JiangsuChina

Objective The aim of this study was to assess the impact of bisphenol A(BPA)and its substitute,bisphenol F(BPF),on the colonic fecal community structure and function of *** We exposed 6–8-week-old male C57BL/6 mice to 5 mg/(kg∙day)and 50μg/(kg∙day)of BPA or BPF for 14 *** samples from the colon were analyzed using 16S rRNA *** Gut microbiome community richness and diversity,species composition,and function were significantly altered in mice exposed to BPA or *** change was characterized by elevated levels of Ruminococcaceae UCG-010 and Oscillibacter and decreased levels of Prevotella 9 and ***,pathways related to carbohydrate and amino acid metabolism showed substantial *** Mice exposed to different BP analogs exhibited distinct gut bacterial community richness,composition,and related metabolic *** the essential role of gut bacteria in maintaining intestinal homeostasis,our study highlights the intestinal toxicity of BPs in vertebrates.

关键词： Bisphenol A(BPA) Bisphenol F(BPF) 16S rRNA Microbiota

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关系叠加视角下的村寨制政治形态——以西南传统侗族村落社会调查为基点

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云南社会科学 2020年第4期 11-17页

作者：秦荣炎华中师范大学政治科学高等研究院

人类社会发展依次经历了原生形态的原始公社制、次生形态的村社制和再生形态的家户制三种形态。在1949年以前的传统时期,中国西南少数民族地区存在一种介于村社制与家户制之间的村寨制过渡形态。在关系叠加视角下,村寨制中的家既作为社... 详细信息

人类社会发展依次经历了原生形态的原始公社制、次生形态的村社制和再生形态的家户制三种形态。在1949年以前的传统时期,中国西南少数民族地区存在一种介于村社制与家户制之间的村寨制过渡形态。在关系叠加视角下,村寨制中的家既作为社会的基本单元,体现着血缘关系;又作为纳税单元与国家、作为构成单元与村寨建立起双重地域关系。由于西南地区远离国家政治中心,相对于国家而言,家户对村寨的依赖程度更高。村寨基本单元孕育了以村寨地域关系为主导的双重权力结构、双层规则体系和村寨主义价值取向,形成村寨共同体的自治传统。村寨制政治形态是西南特殊地域下传统国家成长进程中的阶段性产物,也是村民自治发源于南方少数民族地区并运行较好的重要基础。

关键词：村寨制共同体关系叠加政治形态

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并发病理性黄疸早产低出生体重儿体成分分析

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医学信息 2024年第11期37卷 89-93页

作者：曾婷曾佩佩覃荣娟黄丽萍韦义军袁绍丽邓梁琼柳州市妇幼保健院儿童保健科柳州市妇幼保健院新生儿科广州市妇女儿童医疗中心柳州医院儿童保健科

目的探讨并发病理性黄疸早产低出生体重儿身体成分差异。方法纳入柳州市妇幼保健院2021年8月-2022年2月出生的早产低出生体重儿51例，根据是否并发病理性黄疸分为黄疸组及对照组，分别于婴儿出生后第1～7天采集婴儿身体成分，包括脂... 详细信息

目的探讨并发病理性黄疸早产低出生体重儿身体成分差异。方法纳入柳州市妇幼保健院2021年8月-2022年2月出生的早产低出生体重儿51例，根据是否并发病理性黄疸分为黄疸组及对照组，分别于婴儿出生后第1～7天采集婴儿身体成分，包括脂肪量、瘦体重、水合状态、总身体水分、营养指数等，采用生物电阻抗分析两组婴儿人体成分的差异。结果出生第2天并发病理性黄疸婴儿13例，未并发病理性黄疸38例，黄疸组和对照组婴儿相位角、脂肪及人体总水分含量比较，差异有统计学意义（P0.05）。结论并发病理性黄疸早产低出生体重儿相位角测量值及身体总水分含量低于未并发病理性黄疸婴儿，提示总水分含量越低，相位角检测值越低，婴儿并发病理性黄疸的可能性越大。但由于研究的局限性，相位角及人体总水分含量是否可用于预测早产低出生体重儿并发病理性黄疸的风险尚需进一步研究。

关键词：早产儿低出生体重儿人体成分分析生物电阻抗黄疸

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反刍动物冷应激作用机理及其调控技术研究进展

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饲料研究 2024年第6期47卷 148-153页

作者：李林贵陈翔宇阿不夏合满·穆巴拉克秦荣艳刘艳丰王彦王乐乐王文奇新疆农业大学动物科学学院新疆乌鲁木齐830052 新疆畜牧科学院饲料研究所新疆乌鲁木齐830011

我国北方地区畜牧业具有很大的发展潜力,但冬季持续低温易引起反刍动物的冷应激反应,导致反刍动物的生产性能、免疫力、抗氧化功能降低,给动物生产造成巨大损失。因此,研究反刍动物冷应激的作用机理及其调控技术对北方畜牧业的健康发展... 详细信息

我国北方地区畜牧业具有很大的发展潜力,但冬季持续低温易引起反刍动物的冷应激反应,导致反刍动物的生产性能、免疫力、抗氧化功能降低,给动物生产造成巨大损失。因此,研究反刍动物冷应激的作用机理及其调控技术对北方畜牧业的健康发展具有重要作用。文章综述了反刍动物冷应激的作用机理,以及冷应激对反刍动物生产性能、免疫和血液生理生化指标影响的研究进展,并提出抗冷应激相关的调控技术,为反刍动物冷应激相关的研究提供参考。

关键词：反刍动物冷应激生理功能预防和调控

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Portalsystemic hemodynamic changes in chronic severe hepatitis B: An ultrasonographic study

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World Journal of Gastroenterology 2008年第5期14卷 795-799页

作者： Zhong-Zhen Su Hong Shan Wei-Min Ke Bing-Jun He rong-qin Zheng Department of Infectious Diseases The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou 510630 Guangdong Province China Department of Radiology The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou 510630 Guangdong Province China Department of Ultrasound The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou 510630 Guangdong Province China

AIM： To evaluate portalsystemic hemodynamic changes in chronic severe hepatitis B. METHODS： Hemodynamic parameters included portal vein diameter （PVD）, portal vein peak velocity （PVPV）, portal vein volume （PW）, spleen length （SPL）, spleen vein diameter （SPVD）, spleen vein volume （SPW） and umbilical vein recanalization. They were measured by Color Doppler ultrasonography in 36 patients with chronic severe hepatitis B, compared with 51 normal controls, 61 patients with chronic hepatitis B, 46 patients with compensable cirrhosis, and 36 patients with decompensable cirrhosis. RESULTS： In the group of chronic severe hepatitis B, PVD （12.38 ± 1.23 mm） was significantly different from the normal control, compensable cirrhosis and decompensable cirrhosis groups （P = 0.000-0.026）, but not significantly different from the chronic hepatitis group. PVPV （16.15 ± 3.82 cm/s） dropped more significantly in the chronic severe hepatitis B group than the normal control, chronic hepatitis B and compensable cirrhosis groups （P = 0.000-0.011）. PW （667.53 ± 192.83 mL/min） dropped significantly as compared with the four comparison groups （P = 0.000-0.004）. SPL （120.42 ± 18.36 mm） and SPVD （7.52 ± 1.52 mm） were longer in the normal control and chronic hepatitis B groups （P = 0.000-0.009）, yet they were significantly shorter than those in the decompensable cirrhosis group （P = 0.000）. SPW （242.51 ± 137.70 mL/min） was also lower than the decompensable cirrhosis group （P = 0.000）. The umbilical vein recanalization rate （75%） was higher than the chronic hepatitis B and compensable cirrhosis groups. In the course of progression from chronic hepatitis to decompensable cirrhosis, PVD, SPL and SPVD gradually increased and showed significant differences between every two groups （P = 0.000-0.002）. CONCLUSION： Patients with chronic severe hepatitis B have a tendency to develop acute portal hypertension, resulting in significantly reduced portal vein perfusion, Observation of the portalsystemic h

关键词： Color doppler ultrasonography Portalsystemic hemodynamics Chronic severe hepatitis B

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Vitamin D and selenium for type 2 diabetes mellitus with Hashimoto’s thyroiditis:Dosage and duration insights

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World Journal of Diabetes 2024年第8期15卷 1824-1828页

作者： Yun-Feng Yu Xue-Li Shangguan Dan-Ni Tan Li-Na qin rong Yu School of Traditional Chinese Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina

This letter discusses the publication by Feng et ***,selenium,and vitamin D are closely associated with thyroid hormone production in humans;however,the efficacy of selenium and vitamin D supplementation for type 2 diabetes mellitus(T2DM)patients with Hashimoto’s thyroiditis(HT)remains *** the retrospective study we discuss herein,the authors highlighted significant improvements in thyroid function,thyroid antibodies,blood glucose,and blood lipid in T2DM patients with HT following addition of vitamin D and selenium to their antidiabetic regimens,underscoring the value of these *** team is currently engaged in research exploring the relationship between micronutrients and HT,and we have obtained invaluable insights from the aforementioned *** on this research and current literature,we recommend a regimen of 4000 IU/day of vitamin D and 100-200μg/day of selenium for over three months to six months for patients with HT,particularly for those with concurrent T2DM.

关键词： Vitamin D Selenium Type 2 diabetes mellitus Hashimoto’s thyroiditis Dosage Duration

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