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Developmental stage-specific A-to-I editing pattern in the postnatal pineal gland of pigs(Sus scrofa)

Journal of Animal Science and Biotechnology 2021年第1期12卷 198-206页

作者： rong zhou Wenye Yao Chundi Xie Leixia Zhang Yangli Pei Hua Li Zheng Feng Yalan Yang Kui Li Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education InstitutesSchool of Life Science and EngineeringFoshan UniversityFoshan 528231GuangdongChina State Key Laboratory of Animal Nutrition Key Laboratory of Animal Genetics Breeding and ReproductionMinistry of Agriculture and Rural AffairsInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193China.

Background: RNA editing is a widespread post-transcriptional modification mechanism in mammalian genomes.Although many editing sites have been identified in domestic pigs(Sus scrofa), little is known about the characteristics and dynamic regulation of RNA editing in the pineal gland(PG), a small neuroendocrine gland that synthesizes and secretes melatonin, which is primarily responsible to modulate sleep patterns.Results: This study analyzed the expression of adenosine-to-inosine(A-to-I) editing regulators and profiled the first dynamic A-to-I RNA editome during postnatal PG development. The results identified ADAR1 as the most abundantly expressed ADAR enzyme, which was down-regulated during postnatal PG development. Furthermore,47,284 high-confidence RNA editing sites were identified, the majority of which(93.6%) were of the canonical A-to-I editing type, followed by C-to-T editing. Analysis of its characteristics showed that the A-to-I editing sites mostly localized in SINE retrotransposons PRE-1/Pre0_SS. Moreover, a strong deficiency and preference for guanine nucleotides at positions of one base upstream or downstream were found, respectively. The overall editing level at the puberty stage was higher than at both infancy and adulthood stages. Additionally, genome-wide RNA editing was found to exhibit a dynamic stage-specific fashion(postnatally). Genes that underwent developmental changes in RNA editing were associated with catabolic processes as well as protein localization and transport functions,implying that RNA editing might be responsible for the molecular machineries of the postnatal developing PG.Remarkably, RNA editing in 3′-UTRs might regulate gene expression by influencing miRNA binding during PG development.Conclusions: This study profiles the first comprehensive developmental RNA editome in the pig PG, which contributes to the understanding of the importance of post-transcriptionally mediated regulation during mammalian postnatal PG development. Moreover, this study widely extends RNA editome re

关键词： A-to-I Pig Pineal gland Postnatal development RNA editing

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Microscale endometrial sampling biopsy in detecting endometrial cancer and atypical hyperplasia in a population of 1551 women:a comparative study with hysteroscopic endometrial biopsy

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Chinese Medical Journal 2021年第2期134卷 193-199页

作者： Guo Zhang Yue Wang Xu-Dong Liang rong zhou Xiu-Li Sun Jian-Liu Wang Li-Hui Wei Department of Gynecology and Obstetrics Peking University People’s HospitalBeijing 100044China

Background:Endometrial cancer is one of the most common malignancies of the reproductive system.Effective and cost-effective screening method for populations at high risk is not available.This study aimed to investigate specimen adequacy and the influencing factors in microscale endometrial sampling biopsy and to evaluate the diagnostic accuracy and medical cost of biopsy in endometrial cancer and atypical hyperplasia screenings in comparison with hysteroscopic endometrial biopsy.Methods:A total of 1551 patients at high risk for endometrial lesions who required hysteroscopic endometrial biopsy from November 2017 to August 2018 were included.Microscale endometrial sampling biopsy was performed,followed by hysteroscopic endometrial biopsy.We evaluated the specimen adequacy and influencing factors of microscale endometrial sampling.Diagnostic consistency between microscale endometrial sampling biopsy and hysteroscopic endometrial biopsy was evaluated.The sensitivity,specificity,positive predictive value,and negative predictive value of microscale endometrial sampling biopsy in screening for endometrial cancer and atypical hyperplasia were analyzed,and the medical costs of the two procedures were compared.Results:The specimen adequacy was 81.2%.Patient age,menopausal status,endometrial thickness,and endometrial lesion type were correlated with specimen adequacy.There was good consistency in distinguishing benign and malignant endometrial diseases between microscale endometrial sampling biopsy and hysteroscopic biopsy(kappa 0.950,95%CI 0.925-0.975).The sensitivity,specificity,positive predictive value,and negative predictive value of microscale endometrial sampling biopsy were 91.7%,100.0%,100.0%,and 99.3%for endometrial cancer screening,respectively,and 82.0%,100.0%,100.0%,and 99.4%for atypical hyperplasia screening.The medical cost of endometrial sampling biopsy was only 22.1%of the cost of hysteroscopic biopsy.Conclusions:Microscale endometrial sampling biopsy is a minimally invasive alternative technique for obtai

关键词： Endometrial atypical hyperplasia Endometrial cancer Hysteroscopic endometrial biopsy Microscale endometrial sampling biopsy

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肠道菌群干预肾间质纤维的机制及中药防治研究进展

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辽宁中医杂志 2024年第3期51卷 205-208页

作者：王娟周荣于小勇王乐陕西中医药大学陕西咸阳712046 陕西省中医医院陕西西安710003 陕西省人民医院陕西西安710068

肾间质纤维化(renal interstitial fibrosis,RIF)为慢性肾脏病(chronic kidney disease,CKD)发展成终末期肾脏病的共同病理特征,其发生机制与炎症损伤、氧化应激和细胞自噬等因素密切相关。研究证实肠道菌群与RIF的发生相关,其机制涉及... 详细信息

肾间质纤维化(renal interstitial fibrosis,RIF)为慢性肾脏病(chronic kidney disease,CKD)发展成终末期肾脏病的共同病理特征,其发生机制与炎症损伤、氧化应激和细胞自噬等因素密切相关。研究证实肠道菌群与RIF的发生相关,其机制涉及肠道菌群相关的尿毒症毒素、代谢物质等方面。中药在改善RIF进而保护肾功能等方面具有较好的临床效果。大量研究表明中药可以通过调节肠道菌群进而改善RIF。文章就肠道菌群参与RIF的相关机制以及中药调节肠道菌群防治RIF的相应措施做一总结,为后续肠道菌群作为影响RIF提供些许思路和方向。

关键词：肾间质纤维化肠道菌群机制中药

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Artificial Cycle with or without a Depot Gonadotropin-releasing Hormone Agonist for Frozen-thawed Embryo Transfer： An Assessment of Infertility Type that Is Most Suitable

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Current Medical Science 2018年第4期38卷 626-631页

作者： Di XIE Fan CHEN Shou-zhen XIE Zhi-lan CHEN Ping TUO rong zhou Juan ZHANG Reproductive Medical Centre Wuhan General Hospital of Guangzhou Military Region Wuhan 430070 China Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction Education Ministry of China College of Animal Science and Technology Huazhong Agricultural University Wuhan 430070 China

The clinical outcomes of five groups of infertility patients receiving frozen- thawed, cleavage-stage embryo transfers with exogenous hormone protocols with or without a depot gonadotropin-releasing hormone （GnRH） agonist were assessed. A retrospective cohort analysis was performed on 1003 cycles undergoing frozen-thawed, cleavage-stage embryo transfers from January 1, 2012 to June 31, 2015 in the Reproductive Medicine Center of Wuhan General Hospital of Guangzhou Military Region. Based on the infertility etiologies of the patients, the 1003 cycles were divided into five groups： tubal infertility, polycystic ovary syndrome （PCOS）, endometriosis, male infertility, and unexplained infertility. The main outcome was the live birth rate. Two groups were set up based on the intervention： group A was given a GnRH agonist with exogenous estrogen and progesterone, and group B （control group） was given exogenous estrogen and progesterone only. The results showed that the baseline serum hormone levels and basic characteristics of the patients were not significantly different between groups A and B. The live birth rates in groups A and B were 41.67% and 29.29%, respectively （P〈0.05）. The live birth rates in patients with PCOS in groups A and B were 56.25% and 30.61%, respectively （P〈0.05）. The clinical pregnancy, implantation and on-going pregnancy rates showed the same trends as the live birth rates between groups A and B. The ectopic pregnancy rate was significantly lower in group A than in group B. We concluded that the live birth rate was higher and other clinical outcomes were more satisfactory with GnRH agonist co- treatment than without GnRH agonist co-treatment for frozen-thawed embryo transfer. The GnRH agonist combined with exogenous estrogen and progesterone worked for all types of infertility tested, especially for women with PCOS.

关键词： frozen-thawed embryo transfer gonadotropin-releasing hormone agonist polycystic ovary syndrome

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隧道纵向开挖变形位移监测数值仿真分析

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水力发电 2024年第2期50卷 18-23页

作者：李江沙周荣湖北省地质局地球物理勘探大队湖北武汉430056

隧道纵向开挖变形位移监测数值仿真分析在监测过程中存在位移数据收集失误的情况,检测数值仿真的精准程度较低,在进行仿真的同时需调节位移数据收录系统的监测结构,影响最终的仿真效果。针对此类问题,构建隧道纵向开挖变形位移监测数值... 详细信息

隧道纵向开挖变形位移监测数值仿真分析在监测过程中存在位移数据收集失误的情况,检测数值仿真的精准程度较低,在进行仿真的同时需调节位移数据收录系统的监测结构,影响最终的仿真效果。针对此类问题,构建隧道纵向开挖变形位移监测数值仿真模型,根据地基与隧道的模型结构设置参数,进一步调整预测的变形位移监测数据,精准录入收集的监测信息,获取精确度较高的位移数值,并构建数值仿真模型,完成整体数值仿真分析研究。结果可知,该数值仿真分析方法具有较强的监测精准度,在相同的地表数据沉降拟合条件下,监测的地表沉降值与实际值的误差为0.005%,隧道隆起值与实际值的误差为0.001%,能有效监测隧道变形位移状况,具有较强的监测性能。

关键词：隧道纵向开挖变形位移监测数值仿真分析

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Molecular genetic analysis of phosphomannomutase genes in Triticum monococcum

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The Crop Journal 2015年第1期3卷 29-36页

作者： Chunmei Yu Xinyan Liu Qian Zhang Xinyu He Wan Huai Baohua Wang Yunying Cao rong zhou School of Life Science Nantong University

In higher plants, phosphomannomutase(PMM) is essential for synthesizing the antioxidant ascorbic acid through the Smirnoff–Wheeler pathway. Previously, we characterized six PMM genes(Ta PMM-A1, A2, B1, B2, D1 and D2) in common wheat(Triticum aestivum, AABBDD).Here, we report a molecular genetic analysis of PMM genes in Triticum monococcum(AmAm), a diploid wheat species whose Amgenome is closely related to the A genome of common wheat. Two distinct PMM genes, Tm PMM-1 and Tm PMM-2, were found in T. monococcum. The coding region of Tm PMM-1 was intact and highly conserved. In contrast, two main Tm PMM-2 alleles were identified, with Tm PMM-2a possessing an intact coding sequence and Tm PMM-2b being a pseudogene. The transcript level of Tm PMM-2a was much higher than that of Tm PMM-2b, and a bacterially expressed Tm PMM-2a recombinant protein displayed relatively high PMM activity. In general, the total transcript level of PMM was substantially higher in accessions carrying Tm PMM-1 and Tm PMM-2a than those harboring Tm PMM-1 and Tm PMM-2b. However, total PMM protein and activity levels did not differ drastically between the two genotypes. This work provides new information on PMM genes in T. monococcum and expands our understanding on Triticeae PMM genes, which may aid further functional and applied studies of PMM in crop plants.

关键词： Diploid wheat Phosphomannomutase Allele Gene expression Triticeae

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Low dose bisphenol A impairs spermatogenesis by suppressing reproductive hormone production and promoting germ cell apoptosis in adult rats

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The Journal of Biomedical Research 2013年第2期27卷 135-144页

作者： Pengpeng Jin Xiaoli Wang Fei Chang Yinyang Bai Yingchun Li rong zhou Ling Chen State Key Laboratory of Reproductive Medicine Nanjing Medical University Department of Physiology Nanjing Medical University Department of Physiology Wenzhou Medical College

Bisphenol A （BPA）, an estrogenic chemical, has been shown to reduce sperm count; however, the underlying mechanisms remain unknown. Herein, we show that oral administration of BPA （2 μg/kg） for consecutive 14 days in adult rats （BPA rats） significantly reduced the sperm count and the number of germ cells compared to controls. The serum levels of testosterone and follicle-stimulating hormone （FSH）, as well as the level of GnRH mRNA in BPA rats were lower than those of control rats. Testosterone treatment could partially rescue the reduction of germ cells in BPA rats. Notably, the number of apoptotic germ cells was significantly increased in BPA rats, which was insensitive to testosterone. Furthermore, the levels of Fas, FasL and caspase-3 mRNA in the testicle of BPA rats were increased in comparison with controls. These results indicate that exposure to a low dose of BPA impairs spermatogenesis through decreasing reproductive hormones and activating the Fas/FasL signaling pathway.

关键词： bisphenol A （BPA） spermatogenesis apoptosis testosterone

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多靶区经颅直流电刺激对健康成年人工作记忆-姿势控制双任务表现的影响

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中国康复理论与实践 2024年第1期30卷 21-28页

作者：周治宁周容肖燕文王博文吕娇娇刘宇上海体育大学运动健身科技省部共建教育部重点实验室上海市200438 中山大学孙逸仙纪念医院深汕中心医院广东汕尾市516600

目的探究多靶区经颅直流电刺激(tDCS)和单靶区tDCS对健康成年人工作记忆-姿势控制双任务表现的影响,并比较两种刺激方案的调控效果。方法2020年11月至2021年2月,招募上海体育大学19例健康成年人,随机接受多靶区tDCS、单靶区tDCS和假刺激... 详细信息

目的探究多靶区经颅直流电刺激(tDCS)和单靶区tDCS对健康成年人工作记忆-姿势控制双任务表现的影响,并比较两种刺激方案的调控效果。方法2020年11月至2021年2月,招募上海体育大学19例健康成年人,随机接受多靶区tDCS、单靶区tDCS和假刺激,任意两种刺激之间至少间隔1周。其中,多靶区tDCS的目标靶区为左侧背外侧前额叶(LDLPFC)和双侧初级运动皮质(M1);单靶区tDCS仅刺激L-DLPFC。在每种刺激前后,所有受试者进行单任务和双任务条件下的步行和站立平衡测试,双任务中的第二任务为N-back任务。刺激前后观察双任务姿势控制表现、双任务消耗(DTC)和工作记忆表现。结果刺激后,3种刺激间双任务步行的步幅变异性(F=3.792,P=0.029)、步幅变异性_DTC(F=3.412,P=0.040)以及双任务站立平衡的压心摆动速度(Vcop)(F=3.815,P=0.029)有显著性差异。与假刺激相比,多靶区tDCS后,步幅变异性(P=0.047)和Vcop(P=0.015)降低,步幅变异性_DTC有降低趋势(P=0.073);单靶区tDCS后,步幅变异性(P=0.011)、步幅变异性_DTC(P=0.014)和Vcop(P=0.025)均降低。与单靶区tDCS相比,多靶区tDCS后,压心摆动面积的双任务消耗降低(P=0.035)。3种刺激后受试者工作记忆表现改变无显著性差异(P>0.05)。结论多靶区tDCS和单靶区tDCS均可调控健康成年人的工作记忆-姿势控制双任务表现;且与单靶区tDCS相比,多靶区tDCS在调控站立平衡双任务姿势控制效果上具有一定优势。

关键词：双任务姿势控制工作记忆背外侧前额叶初级运动皮质经颅直流电刺激

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乡村振兴视域下乡村治理研究的进展、热点和展望——基于CNKI(2017—2023年)的文献计量

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青岛科技大学学报（社会科学版） 2024年第2期40卷 47-56页

作者：周镕基廖彪湖南省乡村产业振兴研究中心湖南衡阳421002 衡阳师范学院湖南衡阳421002

以2017—2023年中国知网(CNKI)数据库793篇CSSCI期刊相关论文为数据基础,信息可视化软件CiteSpace为研究手段,对国内乡村治理研究的知识图谱进行可视化分析,揭示乡村治理研究的进展、热点及前沿。研究结果显示:自乡村振兴战略实施以来,... 详细信息

以2017—2023年中国知网(CNKI)数据库793篇CSSCI期刊相关论文为数据基础,信息可视化软件CiteSpace为研究手段,对国内乡村治理研究的知识图谱进行可视化分析,揭示乡村治理研究的进展、热点及前沿。研究结果显示:自乡村振兴战略实施以来,乡村治理研究呈现稳定的增长态势。协同治理与社会参与、现代治理与乡村转型、数字治理与技术应用、土地流转与社会公正构成当前研究热点。然而,现有研究仍面临地域研究分布不平衡、理论体系框架尚待完善、公众参与研究尚需拓展等困境。从内容、视角和方法三个层面展望未来的乡村治理研究,应深入探讨数字治理与乡村转型的结合,以及绿色农业与生态转型的实践,并探索多元主体的合作机制,广泛应用“互联网+监管”与区块链技术。

关键词：乡村治理乡村振兴数字乡村生态转型

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Visual acuity is correlated with ischemia and neurodegeneration in patients with early stages of diabetic retinopathy

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Eye and Vision 2021年第1期8卷 385-395页

作者： Jin Li Yue zhou Feng Chen Yingzi Li rong zhou Chaoming Wu Huankai Yu Zhiyang Lin Ce Shi Gu Zheng Yilei Shao Qi Chen Fan Lu Meixiao Shen School of Ophthalmology and Optometry Wenzhou Medical University270 Xueyuan RoadWenzhou 325027ZhejiangChina The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University Wenzhou 325027ZhejiangChina Department of Ophthalmology The Affiliated Hospital of Yangzhou UniversityYangzhou 225000JiangsuChina

Purpose:We investigated the effects of retinal ischemia,neurodegeneration,and subclinical edema on best-corrected visual acuity(BCVA)in the early stages of diabetic retinopathy(DR).Methods:Ischemia was evaluated by the microvascular parameters measured by optical coherence tomography angiography.Neurodegeneration and subclinical edema were identified by the intraretinal layer thickness obtained by optical coherence tomography.Eyes with nonproliferative diabetic retinopathy(n=132)from 89 patients were analyzed.Eyes were classified as having normal BCVA(n=88[66.7%],Snellen equivalent≥20/20)or decreased BCVA(n=44[33.3%],Snellen equivalent<20/20).The prevalence of ischemia,neurodegeneration,and subclinical edema was explored in patients with and without decreased BCVA,and correlations between BCVA and these pathological pathways were determined.Results:Vessel density in the deep retinal capillary plexus(DRCP)and thickness of ganglion cell layer plus inner plexiform layer(GCL-IPL)were significantly lower in eyes with decreased BCVA compared with eyes with normal BCVA(both P<0.05).In the final multiple regression predictive model,age,DRCP vessel density,and GCL-IPL thickness(all P≤0.044)were predictors of BCVA.DRCP vessel density and GCL-IPL thickness have an interactive effect on visual acuity.The proportions of ischemia and neurodegeneration were significantly higher in eyes with decreased BCVA than in eyes with normal BCVA(P=0.001 and P=0.004,respectively).Conclusion:During the natural course of the early stages of DR,ischemia and neurodegeneration were the main disease pathways associated with visual acuity,and the mechanisms varied among patients.

关键词： Diabetic retinopathy Visual acuity Optical coherence tomography Ischemia Neurodegeneration

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