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Journal of Materials Science & Technology 2022年第5期100卷 110-119页

作者： Huinan Zhao Xinyi Guan feng Zhang Yajing huang Dehua Xia Lingling Hu Xiaoyuan Ji Ran Yin Chun He School of Environmental Science and Engineering Sun Yat-sen UniversityGuangzhou 510275China Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology Guangzhou 510275China Department of Civil and Environmental Engineering The Hong Kong University of Science and TechnologyClear Water BayHong Kong SARChina

In this study,bismuth oxyiodide with coexistence of plasmonic Bi and oxygen vacancy(Bi/BiO_(1-x)I)was successfully prepared and used towards photothermal and photocatalytic disinfection of pathogenic bacteria containing *** Bi and oxygen vacancies in Bi/BiO_(1-x)I induced a surface plasmon effect under the irradiation of simulated solar light from 500-900 nm and promoted the generation of hot electrons and reactive species(^(1)O^(2),h^(+)and·O_(2)^(-)).The catalyst showed promising performance for inactivation of *** K-12,with a 7.2 log inactivated achieved under the optimum conditions.A synergy between photothermal and photocatalytic inactivation was identified and *** mechanisms of *** K-12 destruction were *** destruction of extracellular antioxidant enzymes of *** K-12 was identified after ***,the ***'s membrane and its intracellular contents were attacked by the reactive species(^(1)O^(2),h^(+)and·O_(2)^(-))and the thermal *** work provides useful insights into the rational design of semimetal bismuth-mediated photocatalysts towards effective and sustainable water disinfection.

关键词： Photothermocatalysis Semimetal Bi Disinfection Oxygen vacancy Solar light

三期相CT图像分割模型的鼻咽癌肿瘤靶区自动勾画研究

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中华放射医学与防护杂志 2024年第2期44卷 111-118页

作者：姚国荣沈恺赵峰王思源陆中杰黄科杰严森祥浙江大学医学院附属第一医院放疗科杭州311121 浙江大学信息与电子工程学院杭州310058

目的探究使用基于3D U-Net结合三期相CT图像的分割模型对鼻咽癌肿瘤原发灶(GTVnx)和转移的区域淋巴结(GTVnd)自动勾画的有效性和可行性。方法回顾性收集215例鼻咽癌病例的电子计算机体层扫描(CT),包括平扫期(CT)、增强期(CTC)和延迟期(C... 详细信息

目的探究使用基于3D U-Net结合三期相CT图像的分割模型对鼻咽癌肿瘤原发灶(GTVnx)和转移的区域淋巴结(GTVnd)自动勾画的有效性和可行性。方法回顾性收集215例鼻咽癌病例的电子计算机体层扫描(CT),包括平扫期(CT)、增强期(CTC)和延迟期(CTD)3个期相共计645组图像。采用随机数字表法,将数据集划分为172例训练集和43例的测试集。设置了包括三期相CT图像模型及期相微调模型共计6个实验组:三期相CT图像模型即仅使用平扫期(CT)A1组、仅使用增强期(CTC)A2组、仅使用延迟期(CTD)A3组和同时使用三期相(All)A4组。期相微调模型:CTC微调B1组和CTD微调B2组。使用Dice相似性系数(DSC)和95%豪斯多夫距离(HD95)作为定量评价指标。结果使用三期相CT(A4)进行GTVnd靶区自动勾画相比于仅使用单期相CT(A1、A2、A3)获得更好的勾画效果(DSC:0.67vs.0.61、0.64、0.64,t=7.48、3.27、4.84,P0.05)。在GTVnd的自动勾画中,B2、B3vs.A1模型具有更好的自动勾画精度(DSC:0.63、0.63vs.0.61,t=4.10、3.03,P<0.01;HD95:58.11、50.31 mmvs.79.23 mm,t=2.75、3.10,P<0.01)。结论使用三期CT扫描对于鼻咽癌GTVnd靶区具有更好的自动勾画效果。通过期相微调模型,可以提升平扫CT图像上GTVnd靶区的自动勾画精度。

关键词：鼻咽癌自动勾画放射治疗深度学习卷积神经网络

侧孢短芽胞杆菌JYC 688鉴定及抑菌促生特性研究

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植物保护 2024年第2期50卷 153-162页

作者：黄勋丰加文金春林高家卫邓琳梅张潇方杨艳丽刘霞云南农业大学植物保护学院昆明650201 云南省曲靖市马龙区农业农村局曲靖655199

马铃薯疮痂病是由多种致病链霉菌侵染引起薯块表面结痂导致薯块外观品质下降的病害,防控困难,生物防治是其主要防治措施之一。筛选具有良好拮抗作用的生防菌,可为马铃薯病害的生物防治提供优良的微生物资源。本研究从健康马铃薯植株根... 详细信息

马铃薯疮痂病是由多种致病链霉菌侵染引起薯块表面结痂导致薯块外观品质下降的病害,防控困难,生物防治是其主要防治措施之一。筛选具有良好拮抗作用的生防菌,可为马铃薯病害的生物防治提供优良的微生物资源。本研究从健康马铃薯植株根际土壤中分离获得JYC 688菌株,采用平板对峙和纸碟法筛选验证菌株拮抗性,结合形态学观察、生理生化特性测定及分子生物学手段确定其种属地位,并检测其产生水解酶和嗜铁载体的能力。结果表明,菌株JYC 688是侧孢短芽胞杆菌Brevibacillus laterosporus,其菌株培养液能够显著抑制引起马铃薯疮痂病的酸性疮痂链霉菌Streptomyces acidiscabies和肿胀疮痂链霉菌***的生长,抑菌圈直径分别为27.86 mm和31.90 mm;其对马铃薯晚疫病菌Phytophthora infestans、早疫病菌Alternaria alternata、黑痣病菌Rhizoctonia solani也具有良好的抑制活性;菌株JYC 688能够分泌β-1,3葡聚糖酶、纤维素酶、蛋白酶、嗜铁素等多种抗菌相关物质,可溶解大豆卵磷脂、碳酸钙、碳酸镁3种难溶性化合物,有利于磷、钙、镁3种元素的释放,是一株具有开发潜力的生防菌。

关键词：侧孢短芽胞杆菌鉴定抑菌特性促生特性

Advanced natural products chemistry research in China between 2015 and 2017

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Chinese Journal of Natural Medicines 2018年第12期16卷 881-906页

作者： YANG Guo-Xun MA Guang-Lei LI Hao huang Ting XIONG Juan HU Jin-feng Department of Natural Products Chemistry School of Pharmacy Fudan University

In this review, we intensively focus on the advances in research of natural products(NPs) discovery carried out by domestic scholars in China from 2015 through 2017. In general, a total of 1811 publications(1479 in English and 332 in Chinese) were accumulated regarding newly isolated NPs from plants, microorganisms, and marine sources. As a result, 277 selected papers concerning naturally occurring compounds with extraordinary frameworks, origins, and promising activities were discussed in this review article, mainly organized according to their structural classes and novelties.

关键词： Natural Products Chemistry Novel Structures Research Progress Review

Melanin change of retinal pigment epithelium and choroid in the convalescent stage of Vogt-Koyanagi-Harada disease

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International Journal of Ophthalmology(English edition) 2020年第12期13卷 1928-1932页

作者： Ying huang Ya-Ting Yang Bing Lin Sheng-Hai huang Zu-Hua Sun rong Zhou Ying-Zi Li Xiao-Ling Liu School of Ophthalmology&Optometry and Eye Hospital Wenzhou Medical UniversityWenzhou 325027Zhejiang ProvinceChina

AIM:To observe the melanin change of the retinal pigment epithelium(RPE)and choroid in the convalescent stage of Vogt-Koyanagi-Harada(VKH).METHODS:A retrospective study was performed on 40 eyes of 20 patients in the convalescent stage of *** photography(FP),multi-spectral imaging(MSI),and optical coherence tomography(OCT)were ***:In the VKH convalescent stage,focal RPE melanin accumulation(FRMA)was detected in 34 eyes(85%)on MSI and in 7 eyes(17.5%)on *** was limited to the previous retinal detachment area in all 28 eyes(FRMA was detected in 34 eyes on MSI,which were enrolled,and 6 eyes lacked data in the acute stage).Sunset-glow fundus was detected in 20 eyes(50%)on *** mean density of FRMA in a 1-mm-diameter circular area of the fovea was 0.04±0.07 on MSI,which was significantly correlated with sunset-glow fundus(ρ=0.467,P=0.02).CONCLUSION:In the VKH convalescent stage,FRMA is derived from the RPE melanin change,and sunset-glow fundus is derived from the choroid melanin change.A higher density of FRMA in the fovea and sunset-glow fundus represents more serious depigmentation of melanin.

关键词： melanin retinal pigment epithelium choroid convalescent stage Vogt-Koyanagi-Harada

A flexible and stretchable bionic true random number generator

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Nano Research 2022年第5期15卷 4448-4456页

作者： Yongbiao Wan Kun Chen feng huang Pidong Wang Xiao Leng Dong Li Jianbin Kang Zhiguang Qiu Yao Yao Microsystem and Terahertz Research Center China Academy of Engineering PhysicsChengdu 610200China Institute of Electronic Engineering China Academy of Engineering PhysicsMianyang 621999China School of Electronics and Information Technology State Key Lab of Opto-Electronic Materials&TechnologiesGuangdong Province Key Lab of Display Materials and TechnologiesSun Yat-sen UniversityGuangzhou 510275China

The volume of securely encrypted data transmission increases continuously in modern society with all things *** this end,true random numbers generated from physical sources are highly required for guaranteeing security of encryption and decryption schemes for exchanging sensitive ***,majority of true random number generators(TRNGs)are mechanically rigid,and thus cannot be compatibly integrated with some specific flexible ***,we present a flexible and stretchable bionic TRNG inspired by the uniqueness and randomness of biological *** flexible TRNG film is molded from the surface microstructures of natural plants(e.g.,ginkgo leaf)via a simple,low-cost,and environmentally friendly manufacturing *** our proof-of-principle experiment,the TRNG exhibits a fast generation speed of up to 1.04 Gbit/s,in which random numbers are fully extracted from laser speckle patterns with a high extraction rate of 72%.Significantly,the resulting random bit streams successfully pass all randomness test suites including NIST,TestU01,and *** after 10,000 times cyclic stretching or bending tests,or during temperature shock(-25-80℃),the bionic TRNG still reveals robust mechanical reliability and thermal *** a flexible TRNG shows a promising potential in information security of emerging flexible networked electronics.

关键词： random number generator flexible electronics polydimethylsiloxane(PDMS) bionic microstructure information security

沿病变腰椎神经支配区域电针对退变性腰椎滑脱症术后功能恢复的影响

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辽宁中医杂志 2024年第6期51卷 179-182页

作者：王欢黄相鹏孙金星张翔王凤宋钦鹏刘海军侯海涛山东省文登整骨医院山东威海264200

目的探究沿病变腰椎神经支配区域电针对退变性腰椎滑脱症术后功能恢复的影响。方法采用随机分组法将山东省文登整骨医院2019年1月—2022年2月收治的150例退变性腰椎滑脱症患者分为观察组(75例)与对照组(75例)。对照组采用Cage椎间融合... 详细信息

目的探究沿病变腰椎神经支配区域电针对退变性腰椎滑脱症术后功能恢复的影响。方法采用随机分组法将山东省文登整骨医院2019年1月—2022年2月收治的150例退变性腰椎滑脱症患者分为观察组(75例)与对照组(75例)。对照组采用Cage椎间融合椎弓根螺钉固定术治疗。治疗组则在此基础上联合使用电针治疗。观察两组患者手术前与手术2 d、2周、3个月日本矫形外科协会(Japanese Orthopaedic Association,JOA)评分、视觉模拟评分(visual analogue scale,VAS)、Oswestry功能障碍指数(Oswestry Disability Index,ODI)评价患者的生活质量和血清炎症因子金属蛋白酶3(matrix metalloproteinase-3,MMP-3)、白细胞介素-1β(interleckin,IL-1β)、C反应蛋白(C-reactive protein,CRP)。结果两组患者术后2 d、2周、3个月JOA评分显著高于手术前(P<0.05)。术后2周、3个月治疗组JOA评分显著高于对照组(P<0.05)。两组患者术后2周、3个月VAS评分显著低于手术前(P<0.05)。术后2周、3个月治疗组VAS评分显著低于对照组(P<0.05)。两组患者手术后2 d、2周、3个月ODI评分显著低于手术前(P<0.05)。术后2周,3个月治疗组ODI评分显著低于对照组(P<0.05)。两组患者术后2周、3个月MMP-3、CRP、IL-1β水平显著低于手术前(P<0.05)。术后2周、3个月治疗组MMP-3、CRP、IL-1β水平显著低于对照组(P<0.05)。结论沿病变腰椎神经支配区域进行电针治疗能有效降低术后炎症反应、降低术后疼痛程度、改善腰部功能、提升患者生活质量。

关键词：病变腰椎神经支配区域电针退变性腰椎滑脱症椎弓根螺钉固定联合椎间孔融合术

Microstructural Evolution of Co_(35)Cr_(25)Fe_(30)Ni_(10) TRIP Complex Concentrated Alloy with the Addition of Minor Cu and Its Effect on Mechanical Properties

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Acta Metallurgica Sinica(English Letters) 2022年第8期35卷 1291-1300页

作者： Haoyang Yu Wei Fang Jinfei Zhang Jiaxin huang Jiaohui Yan Xin Zhang Juan Wang Jianhang feng Fuxing Yin School of Materials Science and Engineering Hebei University of TechnologyTianjin 300132China Tianjin Key Laboratory of Materials Laminating Fabrication and Interface Control Technology Tianjin 300132China Institute of Materials and Processing Guangdong Academy of SciencesGuangzhou 510650China

The influences of minor Cu addition(2 and 4 at.%)on the microstructural evolution and room-temperature mechanical property of metastable Co_(35)Cr_(25)Fe_(30)Ni_(10)are systemically investigated in the present *** results indicate that the thermally induced hexagonal close-packed(HCP)phase is absent when Cu was added,due to the increase in stacking fault energy(SFE).The 2%-Cu-added alloys showed the largest total elongation of 69%among the three *** the addition of Cu content reaching 4 at.%,heterogeneous grain structures composed of coarse grains(~9μm)and fine grains(~4μm)and Cu-rich precipitates near the grain boundary are observed,showing the highest yield ***,the segregation state of Cu was quantitatively characterized by electron probe microanalysis(EPMA).And effects of Cu addition on microstructures and tensile properties of(Co_(35)Cr_(25)Fe_(30)Ni_(10))_(100-x) Cu _(x) are also *** findings are beneficial to comprehensively understand the Cu-containing complex concentrated alloys.

关键词： Complex concentrated alloy High entropy alloy Cu addition Microstructures Mechanical properties

Update on techniques for cryopreservation of human spermatozoa

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Asian Journal of Andrology 2022年第6期24卷 563-569页

作者： Chuan huang Yu-Lin Tang Jian-Ling Hu Wen-Jun Zhou Zeng-Hui huang Xue-feng Luo Zheng Li Wen-Bing Zhu Institute of Reproductive and Stem Cell Engineering Basic Medicine CollegeCentral South UniversityChangsha 410000China Reproductive and Genetic Hospital of CITIC-Xiangya Changsha 410000China Department of Andrology Center for Men's HealthShanghai General HospitalShanghai Key Lab of Reproductive MedicineShanghai 200025China

In the 1960s,sperm cryopreservation was developed as a method to preserve ***,techniques for the cryopreservation of human spermatozoa have been widely used in assisted ***,although sperm cryobiology has made notable achievements,the optimal method for the recovery of viable spermatozoa after cryopreservation remains *** sperm quality can be affected by cryoprotectants,ice formation,storage conditions,and osmotic stress during the freezing *** review discusses recent advances in different cryopreservation techniques,cryoprotectants,and freezing and thawing methods during cryopreservation and new indications for the use of cryopreserved spermatozoa.

关键词： cryodamage cryoprotectant dry preservation freezing and thawing sperm cryopreservation

不同多年生稻品种内生细菌群落多样性比较分析

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生物技术通报 2024年第5期40卷 225-236页

作者：孔德婷齐笑含刘兴蕾李丽萍胡凤益黄立钰秦世雯云南大学农学院农业农村部多年生稻生物学与种质创新重点实验室昆明650500

【目的】为了解多年生稻内生细菌微生态的结构和潜在功能,探究多年生稻内生细菌与寄主优良性状的关系。【方法】通过16S rRNA扩增子测序技术比较分析了不同多年生稻品种(PR23、PR25和PR107)内生细菌多样性、物种组成和代谢功能。【结果... 详细信息

【目的】为了解多年生稻内生细菌微生态的结构和潜在功能,探究多年生稻内生细菌与寄主优良性状的关系。【方法】通过16S rRNA扩增子测序技术比较分析了不同多年生稻品种(PR23、PR25和PR107)内生细菌多样性、物种组成和代谢功能。【结果】不同基因型的多年生稻具有不同的内生细菌群落,三者β多样性存在显著差异。多年生稻地下和地上部组织分化形成了不同的内生细菌群落,其地下部内生细菌主要由变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)等组成,而地上部内生细菌主要由变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、厚壁菌门(Firmicutes)等组成。不同多年生稻内生细菌特有扩增子序列变体(amplicon sequence variants,ASVs)数量较多,且具有不同的优势内生细菌属和生物标记物种类。多年生稻内生细菌的代谢功能主要为生物合成、生物降解与利用、解毒、代谢产物前体和能量的产生、甘氨酸途径、大分子修饰和代谢簇。品种间内生细菌的差异代谢功能主要涉及酯类、醇类、糖类等物质的生物合成和降解。【结论】寄主基因型和组织分化影响了多年生稻内生细菌的群落结构、物种组成和代谢功能。

关键词：多年生稻内生细菌群落结构 16S rRNA测序