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SARS-CoV-2 productively infects primary human immune system cells in vitro and in CoviD-19 patients

Journal of Molecular Cell biology 2022年第4期14卷 51-64页

作者： Marjorie C.Pontelli italo A.Castro ronaldo b.martins Leonardo La Serra Flavio P.Veras Daniele C.Nascimento Camila M.Silva Ricardo S.Cardoso Roberta Rosales Rogério Gomes Thais M.Lima Juliano P.Souza brenda C.Vitti Diego b.Caetite Mikhael H.F.de Lima Spencer D.Stumpf Cassandra E.Thompson Louis-Marie bloyet Juliana E Toller-Kawahisa Marcela C.Giannini Leticia P.bonjorno Marial.F.Lopes Sabrina S.batah Li Siyuan Rodrigo Luppino-Assad Sergio C.L.Almeida Fabiola R.Oliveira Maira N.benatti Lorena L.F.Pontes Rodrigo C.Santana Fernando C.Vilar Maria Auxiliadora-martins Pei-Yong Shi Thiago M.Cunha Rodrigo T.Calado JoséC.Alves-Filho Dario S.Zamboni Alexandre T.Fabro Paulo Louzada-Junior Rene D.R.Oliveira Sean P.J.Whelan Fernando Q.Cunha Eurico Arruda Virology Research Center Ribeirao Preto Medical SchoolUniversity of Sao Pauloibeirao PretoSao Paulo 14049-900.Brazil Center of Research in Inflammatory Diseases Ribeirao Preto Medical SchoolUniversity of Sao PauloRibeirao PretoSao Paulo 14049-900Brazil Department of Celland Molecular biology Ribeirao Preto Medical SchoolUniversityof Sao PauloRibeirao PretoSao Paulo 14049-900 Brazil Regional blood Centerof Ribeirao Preto Ribeirao Preto Medical ScholUniversity of Sao PauloRibeirao PretoSao Paulo 14049-900Brazil Department of biochemistry&Molecular biology the University of Texas Medical BranchGalvestonTX7755USA Divisions of Clinical lmmunology Infectious Diseases and Intensive Care UnitRibeirao Preto Medical Schooniversity of Sao PauloRibeirao PretoSao Paulo 14049-900Brazil Department of Pathology Ribeirao Preto Medical Scholniversity of Sao PauloRibeirao PretoSao Paulo 14049-900Brazil Department of Molecular Microbiology.Washington University in St.Louis Saint LouisMO 63110USA

The severe acute respiratory syndrome coronavirus 2(SARS-CovV-2)infection is associated with a hyperinflammatory state and lymphocytopenia,a hallmark that appears as both signature and prognosis of disease severity *** cytokine storm and a sustained inflammatory state are commonly associated with immune cell depletion,it is still unclear whether direct SARS-Cov-2 infection of immune cells could also play a role in this scenario by harboring viral *** found that monocytes,as well as both b and T lymphocytes,were susceptible to SARS-Cov-2 infection in vitro,accumulating double-stranded RNA consistent with viral RNA replication and ultimately leading to expressive T cell *** addition,flow cytometry and immunofluorescence analysis revealed that SARS-Cov-2 was frequently detected in monocytes and b lymphocytes from coronavirus disease 2019(CoVID-19)*** rates of SARS-Cov-2-infected monocytes in peripheral blood mononuclear cells from CoVID-19 patients increased over time from symptom onset,with SARS-CoV-2-positive monocytes,b cells,and CD4+T lymphocytes also detected in postmortem lung *** results indicated that SARS-CoV-2 infection of blood-circulating leukocytes in covID-19 patients might have important implications for disease pathogenesis and progression,immune dysfunction,and virus spread within the host.

关键词： SARS-CoV-2 CoVID-19 lymphocytopenia peripheral blood mononuclear cell(PbMC) lymphocytes monocytes apoptosis

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Spatiotemporal dynamics in butterfly hybrid zones

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Insect Science 2024年第2期31卷 328-353页

作者： Ananda R.Pereira martins Natalie b.Warren W.Owen McMillan Rowan D.H.barrett Redpath Museum McGill University859 Sherbrooke Street WestMontrealQuebecCanada Smithsonian Tropical ResearchInstitute GamboaPanama CityPanama

Evaluating whether hybrid zones are stable or mobile can provide novelinsights for evolution and conservation biology. butterflies exhibit high sensitivity to envi-ronmental changes and represent an important model system for the study of hybrid zoneorigins and maintenance. Here, we review the literature exploring butterfly hybrid zones,with a special focus on their spatiotemporal dynamics and the potential mechanisms thatcould lead to their movement or stability. We then compare different lines of evidenceused to investigate hybrid zone dynamics and discuss the strengths and weaknesses ofeach approach. Our goal with this review is to reveal general conditions associated withthe stability or mobility of butterfly hybrid zones by synthesizing evidence obtained us-ing different types of data sampled across multiple regions and spatial scales. Finally, wediscuss spatiotemporal dynamics in the context of a speciation/divergence continuum, therelevance of hybrid zones for conservation biology, and recommend key topics for futureinvestigation.

关键词： butterflies human impacts hybrid zones movement spatiotemporaldynamics stability

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There is more to the knee joint than just the quadriceps:A systematic review with meta-analysis and evidence gap map of hamstring strength,flexibility,and morphology in individuals with gradual-onset knee disorders

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Journal of Sport and Health Science 2024年第4期13卷 521-536页

作者： Helder S.Lopes Marina C.Waiteman Liliam b.Priore Neal R.Glaviano David M.bazett-Jones ronaldo V.briani Fabio M.Azevedo School of Science and Technology Physical Therapy DepartmentSao Paulo State University(UNESP)Presidente Prudente 19060-900Brazil Department of Kinesiology College of AgricultureHealth and Natural ResourcesUniversity of ConnecticutStorrsCT 06269USA School of Exercise and Rehabilitation Sciences College of Health and Human ServicesUniversity of ToledoToledoOH 43606USA

background:Impairments in hamstring strength,flexibility,and morphology have been associated with altered knee biomechanics,pain,and *** the presence of these impairments in individuals with gradual-onset knee disorders is important and may indicate targets for assessment and *** systematic review aimed to synthesize the literature to determine the presence of impairments in hamstring strength,flexibility,and morphology in individuals with gradual-onset knee ***:Five databases(MEDLINE,Embase,CINAHL,SPORTDiscus,and Web of Science)were searched from inception to September *** studies comparing hamstring outcomes(e.g.,strength,flexibility,and/or morphology)between individuals with gradual-onset knee disorders and their unaffected limbs or pain-free controls were ***-analyses for each knee disorder were ***-level certainty was assessed using the Grading of Recommendations Assessment,Development,and Evaluation,and evidence gap maps were ***:Seventy-nine studies across 4 different gradual-onset knee disorders(i.e.,knee osteoarthritis(OA),patellofemoral pain(PFP),chondromalacia patellae,and patellar tendinopathy)were *** with knee OA presented with reduced hamstring strength compared to pain-free controls during isometric(standard mean difference(SMD)=-0.76,95%confidence interval(95%CI):-1.32 to-0.21)and concentric contractions(SMD=-0.97,95%CI:-1.49 to-0.45).Individuals with PFP presented with reduced hamstring strength compared to painfree controls during isometric(SMD=-0.48,95%CI:-0.82 to-0.14),concentric(SMD=-1.07,95%CI:-2.08 to-0.06),and eccentric contractions(SMD=-0.59,95%CI:-0.97 to-0.21).No differences were observed in individuals with patellar *** with PFP presented with reduced hamstring flexibility when compared to pain-free controls(SMD=-0.76,95%CI:-1.15 to-0.36).Evidence gap maps identified insufficient evidence for chondromalacia patel

关键词： Chondromalacia patellae Knee flexors Knee osteoarthritis Patellar tendinopathy Patellofemoral pain

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Finite element model with imposed slip surfaces for earth mass safety evaluation

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Journal of Central South University 2014年第9期21卷 3627-3638页

作者： Nuno ARAUJO Gaspar J.MACHADO Julio b.martins Hugo M.R.D.SILVA C-TAC Territory Environment and Construction Research Centre School of EngineeringUniversity of Minho Guimaraes 4800-058 Portugal Centre of Mathematics School of Sciences University of Minho Guimaraes 4800-058 Portugal

The study of earth masses requires numerical methods that provide the quantification of the safety factor without requiring detrimental assumptions. For that, equilibrium analysis can perform fast computations but require assumptions that limit its potentiality. Limit analysis does not require detrimental assumptions but are numerically demanding. This work provides a new approach that combines the advantage of both the equilibrium method and the limit analysis. The defined hybrid model allows probabilistic analysis and optimization approaches without the assumption of interslice forces. It is compared with a published case and used to perform probabilistic studies in both a homogeneous and a layered foundation. Analyses show that the shape of the density probability functions is highly relevant when computing the probability of failure, and soil elasticity hardly affects the safety of factor of the earth mass.

关键词： slope stability probabilistic methods reliability genetic algorithms safety factor

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The Triple Combination of Meropenem,Avibactam,and a Metallo-b-Lactamase Inhibitor Optimizes Antibacterial Coverage Against Different b-Lactamase Producers

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Engineering 2024年第7期38卷 124-132页

作者： Zhuoren Ling Alistair James Macdonald Farley Aditya Lankapalli Yanfang Zhang Shonnette Premchand-branker Kate Cook Andrei baran Charlotte Gray-Hammerton Claudia Orbegozo Rubio Edgars Suna Jordan Mathias Jürgen brem Kirsty Sands Maria Nieto-Rosado Maria Mykolaivna Trush Nadira Naznin Rakhi Willames martins Yuqing Zhou Christopher Joseph Schofield Timothy Walsh Department of biology&Ineos Oxford Institute for Antimicrobial Research University of OxfordOxford OX13REUK Chemistry Research Laboratory Department of Chemistry and the Ineos Oxford Institute for Antimicrobial ResearchUniversity of OxfordOxford OX13TAUK Latvian Institute of Organic Synthesis Riga LV-1006Latvia Department of Medical Microbiology Division of Infection and ImmunitySchool of MedicineCardiff UniversityCardiff CF144XNUK Enzymology and Applied biocatalysis Research Center Faculty of Chemistry and Chemical EngineeringBabes-Bolyai UniversityCluj-Napoca 400028Romania

This work explores the potential of a triple combination of meropenem(MEM),a novel metallo-blactamase(MbL)inhibitor(indole-2-carboxylate 58(InC58)),and a serine-b-lactamase(SbL)inhibitor(avibactam(AVI))for broad-spectrum activity against carbapenemase-producing bacteria.A diverse panel comprising MbL-and SbL-producing strains was used for susceptibility testing of the triple combination using the agar dilution *** frequency of resistance(FoR)to MEM combined with InC58 was *** were sequenced and tested for cross resistance,fitness,and the stability of the resistance *** with the double combinations of MEM plus an SbL or MbL inhibitor,the triple combination extended the spectrum of activity to most of the isolates bearing SbLs(oxacillinase-48(OXA-48)and Klebsiella pneumoniae carbapenemase-2(KPC-2))and MbLs(New Delhi metallo-blactamases(NDMs)),although it was not effective against Verona integron-encoded metallo-blactamase(VIM)-carrying Pseudomonas aeruginosa(***)and OXA-23-carrying Acinetobacter baumannii(***).The FoR to MEM plus InC58 ranged from 2.22×10^(-7)to 1.13×10^(-6).The resistance correlated with mutations to ompC and comR,affecting porin C and copper permeability,*** mutants manifested a fitness cost,a decreased level of resistance during passage without antibiotic pressure,and cross resistance to another carbapenem(imipenem)and a b-lactamase inhibitor(taniborbactam).In conclusion,compared with the dual combinations,the triple combination of MEM with InC58 and AVI showed a much wider spectrum of activity against different carbapenemaseproducing bacteria,revealing a new strategy to combat b-lactamase-mediated antimicrobial resistance.

关键词： Carbapenemase Metallo/serine-b-lactamase inhibitor Avibactam Meropenem Antimicrobial resistance

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Mathematical simulation of radial heat transfer in packed beds by pseudohomogeneous modeling

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Particuology 2011年第2期9卷 107-113页

作者： Rodrigo béttega Marcos Flávio Pinto Moreira ronaldo Guimaraes Corrêa José Teixeira Freire Federal University of Uberlandia/School of Chemical Engineering-Av.Jo o Naves ávila 2121 Uberlandia-MGCEP 38400-902Brazil Western Paraná State University-Rua da Faculdade 645 Jardim La SalleToledo-PRCEP 85903-000Brazil Federal University of S o Carlos/Department of Chemical Engineering-Rod.Washington Luiz km 235 S o Carlos-SPCEP 13565-905P.O.Box 676Brazil

Uniform flow regime and constant effective thermal conductivity inside packed beds are commonly accepted in the evaluation of the fluid dynamics and heat transfer in such systems. However, several authors have confirmed the presence of an oscillatory velocity profile caused by the effective contribu~ tion of porosity profile in the fluid dynamic behavior of packed beds, which directly influences the heat transfer inside the beds. This paper describes the application of a pseudo-homogeneous mathematical model for describing heat transfer in packed beds with oscillatory profiles of velocity and porosity, using a radius-dependent model for effective thermal conductivity kr. Several temperature profiles were obtained in a packed bed system with thermal source located on the wall. The simulated temperature and effective thermal conductivity obtained from simulations were compared with experimental data and calculation from a model based on uniform kr fitting. The results indicate that the proposed mathematical modeling was capable of better reDresenting the heat transfer in the packed bed.

关键词： Packed bedHeat transferPseudohomogeneous modeling

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Antioxidants Enzymes Activities and NO Levels in Hemodialysis and Continuous Ambulatory Peritoneal Dialysis—A Comparative Study

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Journal of biosciences and Medicines 2024年第2期12卷 50-59页

作者： Natalia Cristina Aparecida Ribeiro e Ribeiro Anderson martins Tavares Jaslana Hainfellner da Silva João Paulo Lima Daher Salim Kanaan Luciene de Carvalho Cardoso-Weide Laboratory of Neuroplasticity Neurobiology Department Biology Institute Federal Fluminense University Rio de Janeiro Brazil Postgraduate Program in Medical Sciences School of Medicine Federal Fluminense University Rio de Janeiro Brazil School of biology UNIVERSO—Salgado de Oliveira University Rio de Janeiro Brazil Department of Pathology Faculty of Medicine Federal Fluminense University Rio de Janeiro Brazil

It consists of a retrospective study with twenty-two individuals diagnosed with DRC: fourteen HD and eight CAPD, followed up in the dialysis center of Antonio Pedro University Hospital (HUAP) of Fluminense Federal University (UFF), between 2015 and 2017. Fifteen healthy control (HC) subjects were included, without diagnosed CKD. Patients with HIV positive, hepatitis A, b, and C, pregnant, cancer, smokers, alcoholics and those exposed to X-rays in the last 3 months, were excluded. Objectives: As oxidative stress and endothelial dysfunction may be linked to the higher prevalence of CVD in CKD patients, we measured the activities of the antioxidants enzymes: SOD and GPx and total NO levels in the plasma and serum of end-stage CKD patients undergoing dialysis therapy, comparing with the HC group. Methods: Quantification of NO levels was performed by fluorometric kit, while activities of SOD and GPx were determined using kinetic methods. Results: We found higher plasma SOD activity in HD (8.58 U/ml) and CAPD (10.14 U/ml), compared to C (3.73 U/ml) group, while GPx activity was decreased in HD (115.38 nmol/h/ml) and CAPD (122.76 nmol/h/ml) groups compared to HC group (275.83 nmol/h/ml). Total serum NO concentration was decreased in HD (14.09 pmol/μl) and CAPD (10.26 pmol/μl), compared to non-CKD patients (49.65 pmol/μl). Conclusion: Decreased total serum NO and GPx activities may lead to endothelial dysfunction and consequently a higher prevalence of CVD in CKD patients.

关键词： Hemodialysis CAPD SOD GPx NO

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Global prevalence and gender inequalities in at least 60 min of self-reported moderate-to-vigorous physical activity 1 or more days per week:An analysis with 707,616 adolescents

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Journal of Sport and Health Science 2024年第5期13卷 709-716页

作者： Raphael H.O.Araujo AndrúO.Werneck Clarice L.martins Luciana L.barboza Rafael M.Tassitano Nicolas Aguilar-Farias Gilmar M.Jesus Robinson Ramírez-Véelez Riki Tesler Adewale L.Oyeyemi Ellen C.M.Silva Robert G.Weaver Mark S.Tremblay Javier brazo-Sayavera Grégore I.Mielke Danilo R.P.Silva Graduation Program in Health Sciences Londrina State University(UEL)Londrina 86057-970Brazil Center for Epidemiological Research in Nutrition and Health Department of NutritionSchool of Public HealthUniversidade de Sao Paulo(USP)Sao Paulo 01246-904Brazil Department of Physical Education Federal University of ParaıbaParaıba 58051-900Brazil Research Centre of Physical Activity Health and LeisureFaculty of SportsLaboratory for Integrative and Translational Research in Population Health(ITR)University of PortoPorto 4500Portugal Postgraduate Program in Physical Education University of BrasıliaBrasılia 70910-900Brazil Department of Kinesiology and Community Health University of Illinois at Urbana-ChampaignChampaignIL 61801-3841USA Department of Physical Education Sports and RecreationUniversidad de La FronteraTemuco 4680000Chile Department of Health State University of Feira de SantanaFeira de Santana 44036-900Brazil Navarrabiomed Hospital Universitario de Navarra(HUN)Navarra Institute for Health Research(IdiSNA)Universidad Publica de Navarra(UPNA)Pamplona 31006Spain Facultad de Ciencias de la Educacion Unidad Central del Valle del Cauca(UCEVA)TuluaValle del Cauca 763022Colombia Health Management Department Faculty of Heath sciencesAriel UniversityAriel 40700Israel College of Health Solutions Arizona State UniversityPhoenixAZ 85004USA Arnold School of Public Health University of South CarolinaColumbiaSC 29208USA Healthy Active Living and Obesity Research Group Children’s Hospital of Eastern Ontario Research InstituteOttawaON K1H 8L1Canada Department of Pediatrics University of OttawaOttawaON K1H 8L1Canada Department of Health Sciences Carleton UniversityOttawaON K1S 5B6Canada Department of Sports and Computer Science Universidad Pablo de Olavide(UPO)Seville 41013Spain Centro Universitario Regional Noreste Universidad de la RepublicaRivera 40000Uruguay School of Public Health The University of QueenslandBrisbaneQLD 4006Australia Department of Physical Education

background:Guidelines recommend that adolescents should accumulate an average of 60 min per day of moderate-to-vigorous physical activity(MVPA).However,using only this cut-off could hide important *** instance,from a population-level point of view,increasing physical activity for those with no or low physical activity could provide more health benefits than increasing physical activity for those with intermediate ***,including a more sensitive cut-point of≥1 days per week could be an additional strategy for identifying those with low access/opportunities for physical activity ***,the current study aims to estimate the prevalence of≥60 min of MVPA≥1 days per week among adolescents globally,and to describe any relevant gender ***:We used representative datasets from 146 countries/territories collected between 2003 and *** was *** were grouped into younger(≤14 years old)and older(>14 years old)*** Poisson regression models were used to identify the relative differences in≥60 min of MVPA≥1 days per week between boys and girls,and random-effects meta-analysis models were used to identify the pooled *** were stratified by country and ***:Approximately 80%of both younger and older adolescents reported≥60 min of MVPA≥1 days per *** prevalence was≥94%in Europe and Central Asia and North America,while the estimates for the other regions wereboys than girls,with the largest differences occurring among the oldest adolescents(Prevalence ratio_(≤14y)=1.04(95%confidence interval(95%CI)):1.03-1.04)*** ratio_(>14y)=1.09(95%CI:1.08-1.10)).Conclusion:Approximately 8 out of 10 adolescents reported accumulating≥60 min of MVPA≥1 days per week,with notable differences between *** differences were observed in several countries,especially among the oldest *** fo

关键词： Global School-based Student Health Survey Health behaviour Among School-aged Children survey Inequality Surveillance Youth

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Effects of lime addition on geotechnical properties of sedimentary soil in Curitiba,brazil

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Journal of Rock Mechanics and Geotechnical Engineering 2018年第1期10卷 188-194页

作者： Jair A.baldovino Eclesielter b.Moreira Wagner Teixeira ronaldo L.S.Izzo Juliana L.Rose Department of Civil Construction Federal University of Technology-Paraná Campus Curitiba

The soil of the Guabirotuba geological formation（Paraná basin, brazil） has physico-mechanical properties which are not suitable for its utilization in pavement construction, in protection of hillsides and slopes, or as shallow foundation support. Treatment of this soil by lime addition would improve its usability. The present context intends to determine the ratio between the splitting tensile strength（q;）and the unconfined compressive strength（q;） of clayey soil in the metropolitan region of Curitiba City,which has been treated with different lime contents and curing times. The control parameters evaluated include lime content（L）, curing time（t）, moisture content（w）, and ratio of porosity to volumetric lime content（η/L;）. It was observed that the q;/q;ratio is between 0.17 and 0.2 in relation to the curing time,and an exponential relation exists between them. Meanwhile, the unconfined compressive strength of lime-treated soil was found to be approximately four times the initial value.

关键词： Clayey soil stabilization Soil strength Lime-soil mixture Curing time

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Anterior transhepatic approach for total caudate lobectomy including spigelian lobe,paracaval portion and caudate process:A brazilian experience

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Hepatobiliary & Pancreatic Diseases International 2018年第4期17卷 371-373页

作者： Eduardo de Souza martins Fernandes Carlo Alberto Pacilio Felipe Pedreira Tavares de Mello ronaldo de Oliveira Andrade Leandro Moreira Savattone Pimentel Camila Liberato Girão Hepato-Pancreato-biliary and Transplant Surgery Hospital Adventista SilvestreRio de JaneiroBrazil Hospital Universitario Clementino Fraga Filho Universidade Federal do Rio De Janeiro(UFRJ)Rio de JaneiroBrazil

To the Editor:Nowadays,because of the infiltration of cholangiocarcinoma to the parenchyma and/or bile ducts of the caudate lobe,the inclusion of caudate lobe combined with a major hepatectomy remains the gold standard approach for a resectable hilar *** the last years of the 20th century,some authors have begun to report isolated caudate lobe resection for hepatocellular

关键词： HCC Anterior transhepatic approach for total caudate lobectomy including spigelian lobe paracaval portion and caudate process:A brazilian experience

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