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Three-dimensional models: from cell culture to Patient-Derived Organoid and its application to future liposarcoma research

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Oncology research 2025年第1期33卷 1-13页

作者： Sayumi Tahara Sydney Rentsch Fernanda Costas Casal DE Faria Patricia Sarchet roma.Karna Federica Calore Raphael E Pollock Department of Surgery Division of Surgical Oncology The James Comprehensive Cancer Center The Ohio State University Wexner Medical Center Columbus OH 43210 USA. Department of Cancer Biology and Genetics The James Comprehensive Cancer Center The Ohio State University Wexner Medical Center Columbus OH 43210 USA.

Liposarcoma is one of the most common soft tissue sarcomas, however, its occurrence rate is still rare compared to other cancers. Due to its rarity, experiments are an essential approach to elucidate liposarcoma pathobiology. Conventional cell culture-based research (2D cell culture) is still playing a pivotal role, while several shortcomings have been recently under discussion. , mouse models are usually adopted for pre-clinical analyses with expectations to overcome the issues of 2D cell culture. However, they do not fully recapitulate human dedifferentiated liposarcoma (DDLPS) characteristics. Therefore, three-dimensional (3D) culture systems have been the recent research focus in the cell biology field with the expectation to overcome at the same time the disadvantages of 2D cell culture and animal models and fill in the gap between them. Given the liposarcoma rarity, we believe that 3D cell culture techniques, including 3D cell cultures/co-cultures, and Patient-Derived tumor Organoids (PDOs), represent a promising approach to facilitate liposarcoma investigation and elucidate its molecular mechanisms and effective therapy development. In this review, we first provide a general overview of 3D cell cultures compared to 2D cell cultures. We then focus on one of the recent 3D cell culture applications, Patient-Derived Organoids (PDOs), summarizing and discussing several PDO methodologies. Finally, we discuss the current and future applications of PDOs to sarcoma, particularly in the field of liposarcoma.

关键词： Cell culture Liposarcoma Patient-Derived Organoid (PDO) Spheroid Three-dimensional (3D) cell culture

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向日葵黄萎病发病程度与减产之间的定量关系及应用皮下接种作为品…

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国外农学（油料作物） 1995年第2期 54-55,14页

作者： roma.,AB

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GPS与电视信号二者可兼容吗？

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测绘技术 1995年第4期 41-48页

作者： roma.,AJ 周祖渊

１９９１年１１月，飞利蒲实验室（ＰＬ）的地球物理研究会与Ｐｕｒｄｕｅ合作，在密西西比州的Ｉｎｖｅｖｎｅｓｓ附近采用ＧＰＳ测量在６１０米的ＷＡＢＧ电视发射塔周围进行。所获数据用于ＰＬ制定方案研究牛顿重力分离的可能性。数... 详细信息

１９９１年１１月，飞利蒲实验室（ＰＬ）的地球物理研究会与Ｐｕｒｄｕｅ合作，在密西西比州的Ｉｎｖｅｖｎｅｓｓ附近采用ＧＰＳ测量在６１０米的ＷＡＢＧ电视发射塔周围进行。所获数据用于ＰＬ制定方案研究牛顿重力分离的可能性。数据采集范围为距塔８公里的半径内。在２公里以内，六台ＧＰＳ接收机有半数不能锁定ＧＰＳ信号。笔者认为某睦塔发送的微波信号干扰了ＧＰＳ卫星发送的信号。

关键词： GPS 电视信号兼容

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现代生物圈中生命物质的生物地球化学

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国外地质（成都） 1992年第4期 38-45页

作者： roma.,EA 温春齐

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High Impact Polylactide Based on Organosilicon Nucleation Agent

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Chinese Journal of Polymer Science 2024年第6期42卷 787-797页

作者： Daria Pakuła Bogna Sztorch Eliza roma.czuk-Ruszuk Bogdan Marciniec Robert E.Przekop Faculty of Chemistry Adam Mickiewicz University in PoznańUniwersytetu Poznańskiego 861-614PoznańPoland Centre for Advanced Technologies Adam Mickiewicz University in PoznańUniwersytetu Poznańskiego 1061-614PoznańPoland Institute of Biomedical Engineering Faculty of Mechanical EngineeringBialystok University of TechnologyWiejska 45C15-351BialystokPoland

Various sectors of the industry are searching for new materials with specific requirements,providing improved *** study presents novel composite materials based on polylactide that have been modified with the organosilicon compound,(3-thiopropyl)polysilsesquioxane(SSQ-SH).The SSQ-SH compound is a mixture of cage structures and not fully condensed random *** composite materials were obtained through injection *** study includes a comprehensive characterization of the new materials that analyze their functional properties,such as rheology(MFR),mechanical strength(tensile strength,Charpy impact strength),and thermal *** microscopic photos were also taken to analyze the microstructure of the *** addition of a 5%by-weight organosilicon compound to polylactide resulted in a significant increase in MFR by 73.8%compared to the neat *** greatest improvement in impact strength was achieved for the 5%SSQ-SH/PLA composite,increasing it by 32.0 kJ/m^(2)compared to PLA,which represents an increase of up to 187%.The conducted research confirms the possibility of modifying the properties of the polymer by employing organosilicon compounds.

关键词： Octa(3-thiopropyl)silsesquioxane 8SH-POSS Polylactide(PLA) Injection moulding Composites

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Challenge of liver disease in systemic lupus erythematosus:Clues for diagnosis and hints for pathogenesis

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World Journal of Hepatology 2014年第6期6卷 394-409页

作者： Ferno Bessone Natalia Poles Marcelo G roma Gastroenterology and Hepatology Department University of Rosario School of Medicine Institute of Experimental Physiology (CONI-CET-UNR) Faculty of Biochemical and Pharmaceutical SciencesUniversity of Rosario

Systemic lupus erythematosus(SLE) encompass a broad spectrum of liver diseases. We propose here to classify them as follows:(1) immunological comorbilities(overlap syndromes);(2) non-immunological comorbilities associated to SLE; and(3) a putative liver damage induced by SLE itself, referred to as "lupus hepatitis". In the first group, liver injury can be ascribed to overlapping hepatopathies triggered by autoimmune mechanisms other than SLE occurring with higher incidence in the context of lupus(e.g., autoimmune hepatitis, primary biliary cirrhosis). The second group includes non-autoimmune liver diseases, such as esteatosis, hepatitis C, hypercoagulation state-related liver lesions, hyperplasic parenchymal and vascular lesions, porphyria cutanea tarda, and drug-induced hepatotoxicity. Finally, the data in the literature to support the existence of a hepatic disease produced by SLE itself, or the occurrence of a SLE-associated prone condition that increases susceptibility to acquire other liver diseases, is critically discussed. The pathological mechanisms underlying each of these liver disorders are also reviewed. Despite the high heterogeneity in the literature regarding the prevalence of SLE-associated liver diseases and, in most cases, lack of histopathological evidence or clinical studies large enough to support their existence, it is becoming increasingly apparent that liver is an important target of SLE. Consequently, biochemical liver tests should be routinely carried out in SLE patients to discard liver disorders, particularly in those patients chronically exposed to potentially hepatotoxic drugs. Diagnosing liver disease in SLE patients is always challenging, and the systematization of the current information carried out in this review is expected to be of help both to attain a better understanding of pathogenesis and to build an appropriate work-up for diagnosis.

关键词： Systemic lupus erythematosus Lupus hepatitis Esteatosis Regenerative nodular hyperplasia Hepatitis C Autoimmune hepatitis Hepatotoxicity Nonsteroidal anti-inflammatory drugs Methotrexate

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Disease impact on the quality of life of children with inflammatory bowel disease

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World Journal of Gastroenterology 2017年第6期23卷 1067-1075页

作者： Giorgos Chouliaras Daphne Margoni Konstantina Dimakou Smaragdi Fessatou Ioanna Panayiotou Eleftheria roma.Giannikou First Department of Pediatrics National and Kapodistrian University of Athens'Aghia Sophia'Children’s Hospital11527 AthensGreece

To assess the impact of disease characteristics on the quality of life (QOL) in children with inflammatory bowel diseases (IBD).METHODSThis was a cross-sectional study conducted at the First Department of Pediatrics of the University of Athens at the “Aghia Sophia” Children’s Hospital. Children diagnosed with Crohn’s disease (CD) or ulcerative colitis (UC), who were followed as outpatients or during a hospitalization, participated, after informed consent was obtained from their legal representative. QOL was assessed by the IMPACT-III questionnaire. Demographic data and disease characteristics were also collected. Statistical analyses included parametric (Student’s t-test and Pearson’s r) and non-parametric (Mann-Whitney test, Fisher’s test and Spearman’s rho) ***-nine patients (UC: 37, 73.0% females, CD: 62, 51.6% females), aged 12.8 ± 2.6 years were included. Overall, as well as, sub-domain scores did not differ between UC and CD (overall score: 73.9 ± 13.3 vs 77.5 ± 11.2, respectively, P = 0.16). In the entire sample, total score was related to physician’s global assessment (PGA, patients classified as “mild/moderate” active disease had, on average, 14.8 ± 2.7 points lower total scores compared to those “in remission”, P < 0.001) and age at IMPACT completion (Pearson’s r = 0.29, P = 0.05). Disease activity assessed by the indices Pediatric Ulcerative Colitis activity index, Pediatric Crohn’s disease activity index or PGA was significantly associated with all subdomains scores. Presence of extraintestinal manifestations had a negative impact on emotional and social functioning *** activity is the main correlate of QOL in children with IBD, underlining the importance of achieving and sustaining clinical remission

关键词： Inflammatory bowel disease ulcerative colitis Crohn’s disease quality of life IMPACT-III children

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Probing Local Lattice Fluctautions in Cuprates and Manganites by High k-Resolution EXAFS

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中国科学技术大学学报 2001年第3期31卷 289-298页

作者： N.L. Saini (Unitá INFM and Dipartimento di Fisica, Università di roma.“La Sapienza” P.le Aldo Moro 2, 00185 roma. Italy) Unita INFM and Dipartimento di Fisica Universita di Roma“La Sapienza”P 意大利 20 0 1 85

X ray absorption fine structure has been exploited as a tool to study local lattice distortions in the perovskite cuperates and manganites, showing novel phenomena as high temperature superconductivity and colossal magneto resistance. Temperature dependent Cu K \%edge\% and Mn K \%edge\% EXAFS have been used to investigate the local lattice distortions associated with the doped charge at metallic densities in these materials. Temperature dependent correlated Debye Waller factors of the Cu O in the cuprates shows an upturn at the stripe formation temperature T \%so\% . Capabilities of the EXAFS technique have been further demonstrated for the case of the manganites, revealing key information on their metal insulator transition. The EXAFS has been shown to be capable of providing quantitative local distortions that are closely associated with the characteristic properties of these complex materials.

关键词：铜系化合物锰系化合物局域晶格形变 EXAFS

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A Robust, Fully Adaptive Hybrid Level-Set/Front-Tracking Method for Two-Phase Flows with an Accurate Surface Tension Computation

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Communications in Computational Physics 2010年第6期8卷 51-94页

作者： Hector D.Ceniceros Alexandre M.roma.Aristeu Silveira-Neto Millena M.Villar Department of Mathematics University of California Santa BarbaraCA 93106USA Departamento de Matematica Aplicada Universidade de Sao PauloCaixa Postal 66281CEP 05311-970Sao Paulo-SPBrasil Faculdade de Engenharia Mecanica Universidade Federal de UberlandiaCEP 38400-902Uberlandia-MGBrasil

We present a variable time step,fully adaptive in space,hybrid method for the accurate simulation of incompressible two-phase flows in the presence of surface tension in two *** method is based on the hybrid level set/front-tracking approach proposed in[*** and ***,***.,205,391-400,2005].Geometric,interfacial quantities are computed from front-tracking via the immersed-boundary setting while the signed distance(level set)function,which is evaluated fast and to machine precision,is used as a fluid *** surface tension force is obtained by employing the mixed Eulerian/Lagrangian representation introduced in[***,***-Khalik,*** and ***,***.,203,493-516,2005]whose success for greatly reducing parasitic currents has been *** use of our accurate fluid indicator together with effective Lagrangian marker control enhance this parasitic current reduction by several orders of *** resolve accurately and efficiently sharp gradients and salient flow features we employ dynamic,adaptive mesh *** spatial adaption is used in concert with a dynamic control of the distribution of the Lagrangian nodes along the fluid interface and a variable time step,linearly implicit time integration *** present numerical examples designed to test the capabilities and performance of the proposed approach as well as three applications:the long-time evolution of a fluid interface undergoing Rayleigh-Taylor instability,an example of bubble ascending dynamics,and a drop impacting on a free interface whose dynamics we compare with both existing numerical and experimental data.

关键词： Front-tracking immersed boundary method level set method adaptive mesh re-finements semi-implicit methods multilevel multigrid closest point transform semi-backward difference formula

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Phenotypic and molecular diversity-based prediction of heterosis in pearl millet(Pennisetum glaucum L.(R.)Br.)

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The Crop Journal 2018年第3期6卷 271-281页

作者： Shashi Kumar Gupta Thirunavukkarasu Nepolean Chinna Ghouse Shaikh Kedarnath Rai Charles Thomas Hash roma.Rani Das Abhishek Rathore International Crops Research Institute for the Semi-Arid Tropics(ICRISAT) Patancheru 502 324India Indian Agricultural Research Institute(IARI) New Delhi 110012India International Crops Research Institute for the Semi-Arid Tropics(ICRISAT) Niamey BP 12404Niger

Genetic distances between hybrid parents based on phenotypic traits and molecular markers were investigated to assess their relationship with heterosis for grain and stover yield and other traits in pearl millet(Pennisetum glaucum [L.] R. Br.). Fifty-one hybrids developed using 101 hybrid parents(B and R lines) and showing a wide range of genetic distance between their parents based on eight phenotypic traits and 28–38 SSRs were evaluated in two sets for two seasons. The correlation between Euclidean distance(phenotypic distance, ED) and simple matching distance(molecular distance, SM) for parents of both sets was low but positive and significant(r = 0.2, P < 0.001).The correlation of ED in parents with better-parent heterosis for grain yield was similar in both sets(r =0.38, P < 0.05). SM was not correlated with heterosis for grain yield in either set of *** results showed that phenotypic distance could be a better predictor of heterosis than molecular distance. The correlation between phenotypic distance and heterosis was not strong enough to permit the use of phenotypic diversity among parents as a major selection criterion for selection of parental lines displaying high levels of heterosis for grain and stover yield in pearl millet.

关键词： Hybrid parents Agronomic traits Euclidean distance Molecular markers Simple matching distance

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