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Fermentation Kinetics of Media Optimization for the Production of Alpha Amylase by a New Isolate of Aspergillus Oryzae

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微生物学通报 2007年第4期34卷 740-744页

作者： Ikram-ul-Haq roheena abdullah Hamid Mukhtar Muhammad Nauman Aftab Institute of Industrial Biotechnology Government College University Bioinformatics Laboratory Institute of Biophysics Chinese Academy of Sciences

The present study is concerned with the isolation and screening of different strains of Aspergillus oryzae for the production of alpha amylase. Ninety strains were isolated from soil and tested for the production of alpha amylase in shake flasks. Of all the strains tested, Aspergillus oryzae GCB-32 and Aspergillus oryzae GCB-35 gave maximum production of alpha amylase. Different culture media were screened for the production of alpha amylase by these two strains. M1 medium containing starch, yeast extract, NH4Cl, MgSO4·7H2O and CaCl2 gave the maximum production of alpha amylase by both the strains Aspergillus oryzae GCB-32 and Aspergillus oryzae *** analysis revealed that the values of product yield coefficient（Y p/x ） and specific product yield coefficient（qp） were found highly significant（p≤0.05） when medium M1 was used for the enzyme production.

关键词：发酵动力学曲霉菌 α淀粉酶隔离种最优化媒介

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A Study of Zoledronic Acid as Neo-Adjuvant, Perioperative Therapy in Patients with Resectable Pancreatic Ductal Adenocarcinoma

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Journal of Cancer Therapy 2013年第3期4卷 797-803页

作者： Dominic E. Sanford Matthew R. Porembka roheena Z. Panni Jonathan B. Mitchem Brian A. Belt Stacey M. Plambeck-Suess Goldie Lin David G. DeNardo Ryan C. Fields William G. Hawkins Steven M. Strasberg Craig Lockhart Andrea Wang-Gillam Simon Peter Goedegebuure David C. Linehan Alvin J. Siteman Cancer Center St. Louis USA Department of Medicine School of Medicine Washington University St. Louis USA Department of Surgery School of Medicine Washington University St. Louis USA

Background: Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy characterized by abundant granulocytic myeloid-derived suppressor cells (G-MDSC = CD45+/Lin﹣/CD33+/CD11b+/CD15+), which infiltrate tumors and suppress anti-tumor immunity. We have previously demonstrated in a murine model of PDAC that zoledronic acid (ZA) depletes G-MDSC resulting in decreased tumor growth and improved survival. We report here the results of a phase 1 clinical trial (NCT00892242) using ZA as neo-adjuvant, perioperative therapy in patients with non-metastatic, resectable pancreatic adenocarcinoma. Methods: Eligible PDAC patients received ZA (4 mg) IV 2 weeks prior to surgery. Patients then received 2 additional doses of ZA 4 weeks apart. Blood and bone marrow were obtained from patients prior to treatment with ZA and 3 months after surgery for analysis of G-MDSC by flow cytometry. Results: Twenty-three patients received pre-operative ZA with at least 6 months of follow-up. Only 15 PDAC patients had nonmetastatic PDAC, which was amenable to resection. ZA was well tolerated, and all adverse events were grade 1 or 2. The most common adverse events were fatigue, abdominal pain/discomfort, anorexia, and arthralgia. Of resected PDAC patients treated with ZA, 1- and 2-year overall survival (OS) was 85.7% and 33.3%, respectively, with a median OS of 18 months. This group had a 1- and 2-year progression-free survival (PFS) of 26.9% and 8.9%, respectively, with a median PFS of 12 months. The prevalence of G-MDSC was unchanged in the blood and bone marrow of PDAC patients pre- and post-treatment with ZA. Conclusion: ZA is safe and well tolerated as neo-adjuvant, peri-operative therapy in PDAC patients. In this small study, we did not observe a difference in OS or PFS compared to historical controls. Also, there was no difference in the prevalence of G-MDSC in the blood and bone marrow of PDAC patients pre- and post-treatment with ZA.

关键词： Pancreatic Cancer Zoledronic Acid Myeloid-Derived Suppressor Cells

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