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Improved Method for Isolation of Microbial RNA from Biofuel Feedstock for Metatranscriptomics

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Advances in Microbiology 2013年第1期3卷 101-107页

作者： Hailan Piao Lye Meng Markillie David E. Culley roderick I. Mackie Matthias Hess Department of Animal Sciences Institute for Genomic Biology and Energy Biosciences University of Illinois Urbana USA DOE Joint Genome Institute Walnut Creek USA Pacific Northwest National Laboratory Richland USA School of Molecular Biosciences Washington State University Richland USA

Metatranscriptomics—gene express profiling via DNA sequencing—is a powerful tool to identify genes that are actively expressed and might contribute to the phenotype of individual organisms or the phenome (the sum of several phenotypes) of a microbial community. Furthermore, metatranscriptome studies can result in extensive catalogues of genes that encode for enzymes of industrial relevance. In both cases, a major challenge for generating a high quality metatranscriptome is the extreme lability of RNA and its susceptibility to ubiquitous RNAses. The microbial community (the microbiome) of the cow rumen efficiently degrades lignocelullosic biomass, generates significant amounts of methane, a greenhouse gas twenty times more potent than carbon dioxide, and is of general importance for the physiological wellbeing of the host animal. Metatranscriptomes of the rumen microbiome from animals kept under different conditions and from various types of rumen-incubated biomass can be expected to provide new insights into these highly interesting phenotypes and subsequently provide the framework for an enhanced understanding of this socioeconomically important ecosystem. The ability to isolate large amounts of intact RNA will significantly facilitate accurate transcript annotation and expression profiling. Here we report a method that combines mechanical disruption with chemical homogenization of the sample material and consistently yields 1 mg of intact RNA from 1 g of rumen-incubated biofuel feedstock. The yield of total RNA obtained with our method exceeds the RNA yield achieved with previously reported isolation techniques, which renders RNA isolated with the method presented here as an ideal starting material for metatranscriptomic analyses and other molecular biology applications that require significant amounts of starting material.

关键词： RNA Extraction Biofuels CAZymes Metatranscriptomics Systems Microbiology Microbial Ecology

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北齐佛教造像的色彩

敦煌研究 2024年第4期 67-72页

作者：韦陀刘勤(译) 伦敦大学亚非学院英国伦敦敦煌研究院西千佛洞文物保护研究所甘肃敦煌736200

响堂山石窟是与云冈石窟、龙门石窟并称的古代三大皇家石窟造像群之一,最初开凿于北齐时代,后经隋、唐、宋、元、明各代加以增凿。笔者通过对响堂山石窟、邺城、曲阳佛教造像以及响堂山北齐石窟中所使用色彩的调查比较,对响堂山石窟北... 详细信息

响堂山石窟是与云冈石窟、龙门石窟并称的古代三大皇家石窟造像群之一,最初开凿于北齐时代,后经隋、唐、宋、元、明各代加以增凿。笔者通过对响堂山石窟、邺城、曲阳佛教造像以及响堂山北齐石窟中所使用色彩的调查比较,对响堂山石窟北齐洞窟中所见色彩进行了初步考察研究。

关键词：响堂山石窟水浴寺北齐邺城曲阳

GROWTH AND INNONVATION OF LAND TRUST SYSTEM IN CHINA

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China Legal Science 2019年第4期7卷 27-53页

作者： Yu Chaoyin Robert D. roderick Shandong University of Finance and Economics Law School 不详

From the beginning of the 21st Century, various forms of land trusts began to appear in some areas of China as the result of rural land reform. Land trust models all have defects which result in peasants contributing their rights to land contractual management but not benefitting fully from the increased agricultural production that ensues. They are not precisely settlors under existing land trust models so they are not able to exercise many control rights normally available to trust settlors. Nor are they exactly the beneficiaries so they do not get the full economic benefit from the rights they contribute. The key challenge for Chinese land trust is that trustee cannot easily negotiate with individual peasants and the land trust models cannot easily negotiate with individual peasants. Unit trust or similar models may be an avenue to avoid the transaction cost difficulties, an approach that does indeed seem promising.

关键词：土地信托制度创新实践成长路径中国农村土地改革个体农民土地经营权 21世纪

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工业历史的记忆——钢铁博物馆

景观设计 2008年第6期 24-29页

作者： roderick Wyllie 张璐（翻译）王江（版式设计）

1986年，蒙特雷市政府收回了占地15000m^2并已废弃的钢铁厂。多年后Surfacedesi Inc与Harari Arquilectos联手将摇摇欲坠的高炉和一片棕地改建成一座现代历史博物馆。博物馆坐落在现代化的芬迪多拉公园中每年接待超过200万的来访游客．... 详细信息

1986年，蒙特雷市政府收回了占地15000m^2并已废弃的钢铁厂。多年后Surfacedesi Inc与Harari Arquilectos联手将摇摇欲坠的高炉和一片棕地改建成一座现代历史博物馆。博物馆坐落在现代化的芬迪多拉公园中每年接待超过200万的来访游客．博物馆向人们讲述着蒙特雷市辉煌悠久的钢铁生产历史．令老一辈回味，更使年轻人铭记。

关键词：历史博物馆钢铁厂生产历史市政府现代化钢铁博物馆

ON THE COORDINATED DEVELOPMENT OF PUBLIC NATURE AND COMMERCIAL NATURE OF LIABILITY INSURANCE

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China Legal Science 2019年第4期7卷 80-109页

作者： Luo Can Robert D. roderick School of Artificial Intelligence and Law in Southwest University of Political Science and Law 不详

The practical problem that the development of China’s liability insurance face can be attributed to the contradiction between public nature and commercial nature. Judging from the development history and the four-dimensional attribute of modern insurance, there is no contradiction between the two and the coordinated development has necessity and feasibility. The coordinated development of the insurance transaction fairness and contract freedom, the insurance public policy and operation technology should be the basic standard. In the future, the system will be optimized from straightening out the relationship between the liability insurance law and the tort liability law, innovation of insurance products, moderate expansion of compulsory insurance, the commercial operation of policy insurance and the improvement of insurance regulatory mechanism, etc.

关键词：责任保险制度商业性公共性保险监管机制侵权责任法商业化操作制度发展现代保险

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重新审视信任

管理@人 2009年第8期 16-19页

作者： roderick M.Kramer 霍娜斯坦福商业研究生院

过去20年中,信任被认为是促进经济迅速发展的最有力的润滑剂和加速器。流行的商业管理书籍大力地渲染着信任的价值和作用;专业的管理学家也都肯定了信任的好处。

关键词：信任感重新审视道夫人类华尔街润滑剂加速器商业管理社会心理学经济迅速发展

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用密度体预测油气

油气地球物理 2006年第1期4卷 52-54页

作者： roderick W Van Koughnet 李霞

密度反演不受常规预测技术许多缺陷的限制,估算的远景构造规模与实际的油气聚集规模非常接近。密度数据很少有错误的含烃指示,预测时间不会浪费在评估错误的情况上,因此其预测更容易、更快捷、更可信。

关键词：密度体密度差异反演烃类预测

THE 40 YEARS DEVELOPMENT AND PROSPECTS OF CRIMINOLOGY RESEARCH IN CONTEMPORARY CHINA

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China Legal Science 2019年第5期7卷 113-138页

作者： Zhang Xiaohu Robert D. roderick(Revised) Law School and Research Center for Criminal Jurisprudence of Renmin University of China 不详

I. INTRODUCTION Since the founding of the Peoples Republic of China (PRC), the theoretical system of Chinese criminology has been gradually established, developed and enriched. Especially along with the progress of reform and opening-up in 1978, the theoretical research of Chinese criminology has been progressing rapidly. Professional research groups and institutions were established;the academic atmosphere of criminology research is becoming strong;and criminology in the position of criminal science is increasingly becoming prominent. The knowledge system of criminology was gradually formed. Description and evaluation of crime phenomena are more objective than before. Relatively great development in the theories regarding the essence, causation and countermeasure of crime has been achieved. Great progress has also been made in the practice of crime prevention and crime control.

关键词： countermeasure of crime crime prevention along with

ON THE EXECUTION SOCIALIZATION PATH OF SOLVING LAW ENFORCEMENT DIFFICULTY

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China Legal Science 2019年第5期7卷 24-51页

作者： Deng Wei Robert D. roderick(Revised) Southwest University of Political Science and Law 不详

In essence, the problem of law enforcement difficulty is a social issue, not just solely on the court. To achieve effective and long-term solutions to the problem, it is necessary to incorporate it into the process of social governance and involve the efforts of the whole society. The path of realization of this concept is given utmost attention in practice. The socialized path of execution is based on the dual drive, the execution demand from the supply side and from the demand side, as the power source. In the meantime, the system is also based on the diversified dispute resolution mechanism and the long-term mechanism for resolution of the enforcement difficulty.

关键词：社会化理念执行难路径社会治理多元化纠纷社会问题化解机制 “难”