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Phenotypic Characterization of Carbapenemase-Producing Enterobacteriaceae Strains in a Referral Teaching Hospital in Yaoundé, Cameroon

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Open Journal of Medical Microbiology 2023年第1期13卷 52-67页

作者： Cecile Ingrid Djuikoue Paule Dana Djouela Djoulako rod.igue kamga wouambo Charlène Nkouankou Tomi Christiane Possi Kiyang Murielle Chantale Tchitchoua Vynnie Manuella Nyatchoutou Blondelle Kitio Messeu Herman Koyouo Tagne Cedric Dylan Seugnou Nana Nadjia Benhamed Hortense Gonsu kamga Benjamin D. Thumamo Pokam Faculty of Health Sciences Université des Montagnes Bangangté Cameroon Faculty of Medicine Sorbonne Université Paris France Division of Hepatology Department of Medecine II Leipzig University Medical Center Leipzig Germany University of Sciences and Technology Oran Algeria Bacteriology Unit University Teaching Hospital Yaounde Cameroon Department of Medical Laboratory Science Faculty of Health Sciences University of Buea Buea Cameroon

Background: Carbapenemase-producing Enterobacteriaceae (CPE) are an important and increasing threat to global health. They are nowadays more encountered routinely in hospitals and cause high morbidity and mortality due to limited therapeutic alternatives. This study sought to determine the prevalence of CPE in Yaoundé teaching hospital, Cameroon, and the associated risk factors. Materials and Method: To achieve this goal, a descriptive cross-sectional study coupled to an analytical component with consecutive collection of Enterobacteria strains was carried out during a three-month period (from 27th July to 24th October 2018) in the University Teaching Hospital of Yaoundé, Cameroon. The oxidase and biochemical identification tests using a miniaturized Api 20 E system were performed on colonies grown on Eosin Methylene Blue (EMB) medium and subcultured on nutrient agar. Drug susceptibility testing was carried out according to the Antibiogram Committee of the French Society of Microbiology (CA-SFM 2018.V.2.0). The detection of carbapenemase production was performed by the CA-SFM 2018 algorithm for the screening of carbapenemase-producing enterobacteriaceae and its classification by inhibitory synergy tests. Results: Out of the 104 isolates, Escherichia coli (50%) was the most prevalent species, followed by Klebsiella pneumoniae (37.5%) and Citrobacter frendii (12.5%). Drugs susceptibility patterns showed a high resistance to penicillins group (97.4% to amoxicillin), cephalosporins (68.4% to cefotaxim, 58.1% to cefixim, 60.7% to ceftazidim, 57.1% of cefoxitin) and aztreonam (55.7%). However, 11.9% carbapenems related resistance was noticed: 14.4% to imipenem, 13.8% to ertapenem and 7.5% to meropenem. Numerous co-resistance to quinolones (65.8%), fluoroquinolones (49.6%), aminoglycosides (49.6%) and cotrimoxazole (71.8%) were also observed. From 104 isolates, AmpC production represented 23.08% (25/104) and 36.54% (38/104) were ESBL-isolates. The ove

关键词： Enterobacteriaceae Antibiotics Carbapenemase Resistance

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Phenotypic Characterization and Prevalence of Carbapenemase-Producing Acinetobacter baumanii Isolates in Four Health Facilities in Cameroon

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Open Journal of Medical Microbiology 2023年第3期13卷 234-249页

作者： Cécile Ingrid Djuikoue Benhamed Nadjia Paule Dana Djouela Djoulako Celianthe Guegang Cedric Seugnou Nana Hélène Valérie Same Njanjo Yemina Grace Kombeu Christiane Kiyang Possi rod.igue kamga wouambo Gandhi Ndieffouo Fosso Merimé Joel Tchinda Talokou Bianca Tchounga Chimi Olivier Pomte Agbor Michael Benjamin D. Thumamo Pokam Nicolas Antoine-Moussiaux Department of Public Health Faculty of Health Sciences of the Université des Montagnes Bangangté Cameroon American Society for Microbiology Bangangte Cameroon Université des Sciences et de la Technologie d’Oran Mohamed Boudiaf Oran Algeria Faculty of Medicine Sorbonne Université Paris France Estuary Academic and Strategic Institute (IUEs/INSAM) Douala Cameroon Faculty of Medicine and Pharmaceutic Sciences University of Douala Douala Cameroon Division of Hepatology Department of Medicine II Leipzig University Medical Center University of Leipzig Leipzig Germany Centre Hospitalier Dominicain St Martin de Porres Yaounde Cameroon Department of Medical Laboratory Science Faculty of Health Sciences University of Buea Buea Cameroon Faculty of Veterinary Medicine University of Liège Liège Belgium

Background and Objective: Nowadays, the clinical utility of carbapenems is threatened by the emergence of resistant bacteria, favored by its increasing use. According to the WHO, Acinetobacter baumannii: nosocomial infection agent, tops the list of priority antibiotic-resistant pathogens, considered to be the riskiest for humans. This study sought to determine the prevalence of carbapenemase-producing Acinetobacter baumannii strains in four health facilities in the Center and Littoral regions of Cameroon and the associated risk factors. Materials and Method: An analytical cross-sectional study was conducted over a six-month period from January to June 2022. All suspicious A. baumanii isolates obtained from pathological samples at the bacteriology laboratory of the different health facilities were systematically collected and re-identified. Re-identification and antimicrobial susceptibility Testing (AST) were performed using the VITEK 2 System and the Kirby-Bauer method according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI). Detection and phenotypic characterization of carbapenemases was performed according to adequate standard procedures. Results: A total of 168/226 clinical isolates of Acinetobacter baumannii were confirmed after re-identification, among which 52.69% derived from male patients, 55.09% from participants aged between 10 - 39 years old, and 46.71% from pus samples. A very high resistance rates to all families of antibiotics was noted, except to colistin (10.2%). 40.12% of these isolates produced carbapenemase, represented by 62.69% of class B and 37.31% of class A. Carbapenemase production was observed only at HMR1, Centre region and at Laquintinie hospital, Littoral region with 53.33% and 50% respectively, even if there is no significant difference (P = 0.81). In addition, frequent hospitalisation was significantly associated to the production of carbapenemase among A. baumanii (Adjusted-OR = 16.53, P-value 0.0001). Con

关键词： Acinetobacter baumannii Resistance Carbapenemases Health Facilities

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Phenotypic Characterization of Carbapenemase-Producing Enterobacteriaceae Strains in a Referral Teaching Hospital in Yaoundé, Cameroon

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Open Journal of Medical Microbiology（医学微生物学） 2023年第1期

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Phenotypic Characterization and Prevalence of Carbapenemase-Producing Acinetobacter baumanii Isolates in Four Health Facilities in Cameroon

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Open Journal of Medical Microbiology（医学微生物学） 2023年第3期

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