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Novel Immunogenic Epitopes in the NaPi-IIb Protein: Production of Monospecific Antibodies Using Synthetic Peptides Outlined on Isoform Specific Regions of the Type IIb Sodium-Dependent Phosphate Transporter (NaPi-IIb)

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Journal of Cancer Therapy 2016年第2期7卷 129-151页

作者： Ângela Alice Amadeu Megale Jorge Mário da Costa Ferreira Júnior Letícia Batista Azevedo Rangel Maria Del Pilar Estevez Diz Max Senna Mano Paulo Marcelo Gehm Hoff Priscila Hess Lopes roberto jun arai Wilmar Dias da Silva Laboratório de Imunoquímica Instituto Butantan S&atildeo Paulo Brazil Departamento de Ciências Farmacêuticas Universidade Federal do Espírito Santo Espírito Santo Brazil Departamento de Pesquisa Clínica Instituto do C&acircncer do Estado de S&atildeo Paulo S&atildeo Paulo Brazil

NaPi-IIb is a multiple passage protein membrane which is primarily responsible for phosphate uptake in the kidney and in the small intestine. Beyond its physiological functions, their involvement with carcinogenesis was initially described in mid-2003, due to its distinct level of expression in normal and tumor cells of the ovary. Although less common than cervical cancer, epithelial ovarian cancer is considered the most lethal gynecologic malignancy, which is mainly due to diagnosis in the advanced stages as a result of the absence of symptoms during the onset of the disease and the lack of tools for early detection. Here, we produce antibodies that are anti-synthetic peptides that are derived from the regions of second extracellular loop of NaPi-IIb, which is a non-overlapping portion of MX35 epitope. These two 15 distinct amino acid residue peptides, designated as Let#1 and Let#2, are engineered in a very thorough way to detect specific sites only in this isoform, thus excluding cross-reactivity with other carriers of the same family. The lack of immunogenicity of small peptides is surpassed by the conjugation with carrier proteins. Using immunochemical methods, we demonstrate that polyclonal antibodies that are mono-specific for the Let#1 and Let#2 peptides recognize proteins that express similar amino acid sequences during blood circulation. Additionally, using flow cytometry, we identify NaPi-IIb in NIH:OVCAR-3 cells. The clear identification of two shorter peptides on the extracellular loop of NaPi-IIb, which are far from the monoclonal antibody MX35-recognizing epitopes, adds new promising tools for ovarian cancer follow-up and staging.

关键词： NaPi2b Peptides Antibodies Ovarian Cancer

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Utility of plasma D-dimer for diagnosis of venous thromboembolism after hepatectomy

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World Journal of Clinical Cases 2024年第2期12卷 276-284页

作者： Taiichiro Miyake Hiroaki Yanagimoto Daisuke Tsugawa Masayuki Akita Riki Asakura Keisuke arai Toshihiko Yoshida Shinichi So jun Ishida Takeshi Urade Yoshihide Nanno Kenji Fukushima Hidetoshi Gon Shohei Komatsu Sadaki Asari Hirochika Toyama Masahiro Kido Tetsuo Ajiki Takumi Fukumoto Division of Hepato-Biliary-Pancreatic Surgery Department of SurgeryKobe University Graduate School of MedicineKobe 650-0017HyogoJapan

BACKGROUND Venous thromboembolism(VTE)is a potentially fatal complication of *** use of postoperative prophylactic anticoagulation in patients who have undergone hepatectomy is controversial because of the risk of postoperative ***,we hypothesized that monitoring plasma D-dimer could be useful in the early diagnosis of VTE after *** To evaluate the utility of monitoring plasma D-dimer levels in the early diagnosis of VTE after *** The medical records of patients who underwent hepatectomy at our institution between January 2017 and December 2020 were retrospectively *** were divided into two groups according to whether or not they developed VTE after hepatectomy,as diagnosed by contrast-enhanced computed tomography and/or ultrasonography of the lower *** factors,including demographic data and perioperative D-dimer values,were compared between the two *** operating characteristic curve analysis was performed to determine the D-dimer cutoff *** and multivariate analyses were performed using logistic regression analysis to identify significant *** In total,234 patients who underwent hepatectomy were,of whom(5.6%)were diagnosed with VTE following hepatectomy.A comparison between the two groups showed significant differences in operative time(529 vs 403 min,P=0.0274)and blood loss(530 vs 138 mL,P=0.0067).The D-dimer levels on postoperative days(POD)1,3,5,7 were significantly higher in the VTE group than in the non-VTE *** the multivariate analysis,intraoperative blood loss of>275 mL[odds ratio(OR)=5.32,95%confidence interval(CI):1.05-27.0,P=0.044]and plasma D-dimer levels on POD 5≥21μg/mL(OR=10.1,95%CI:2.04-50.1,P=0.0046)were independent risk factors for VTE after *** Monitoring of plasma D-dimer levels after hepatectomy is useful for early diagnosis of VTE and may avoid routine prophylactic anticoagulation in the

关键词： Hepatectomy Malignant tumor Postoperative complication D-dimer Early diagnosis Venous thromboembolism

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Protein-losing enteropathy caused by a jejunal ulcer after an internal hernia in Petersen's space: A case report

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World Journal of Clinical Cases 2022年第1期10卷 323-330页

作者： Tomohiko Yasuda Nobuyuki Sakurazawa Komei Kuge jun Omori Hiroki arai Daisuke Kakinuma Masanori Watanabe Hideyuki Suzuki Katsuhiko Iwakiri Hiroshi Yoshida Department of Gastrointestinal Surgery Nippon Medical School Chiba Hokusoh HospitalChiba 270-1694Japan Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery Nippon Medical SchoolTokyo 113-8603Japan Department of Gastroenterology Nippon Medical SchoolTokyo 113-8603Japan

BACKGROUND The incidence of internal hernias has recently increased in concordance with the popularization of laparoscopic *** particular concern are internal hernias occurring in Petersen's space,a space that is surgically created after treatment for gastric cancer and *** hernias cause devastating sequelae,such as massive intestinal necrosis,fatal Roux limb necrosis,and superior mesenteric vein *** addition,protein-losing enteropathy(PLE)is a rare syndrome involving gastrointestinal protein loss,although its relationship with internal Petersen’s hernias remains *** SUMMARY A 75-year-old man with a history of laparotomy for early gastric cancer developed Petersen's hernia 1 year and 5 mo after *** was successfully treated by reducing the incarcerated small intestine and closure of Petersen’s defect without resection of the small *** 3 mo after his surgery for Petersen’s hernia,he developed bilateral leg edema and *** was diagnosed with PLE with an alpha-1 antitrypsin clearance of 733 mL/24 ***-balloon enteroscopy revealed extensive jejunal ulceration as the etiology,and it facilitated minimum bowel *** analysis showed extensive jejunal ulceration and collagen hyperplasia with nonspecific inflammation of all layers without lymphangiectasia,lymphoma,or vascular *** postoperative course was unremarkable,and his bilateral leg edema and hypoalbuminemia improved after 1 *** was no relapse over the 5-year follow-up *** PLE and extensive jejunal ulceration may occur after Petersen's *** enteroscopy helps identify and resect these lesions.

关键词： Internal hernia Ulcer Protein-losing enteropathy Double-balloon enteroscopy Ischemia-reperfusion injury Case report

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一种新型网膜对SARS冠状病毒的抑制作用

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生物化学与生物物理进展 2004年第11期31卷 982-985页

作者：韩伟张泮河曹务春杨东玲 SHIGEHARU TAIRA YOSHIO OKAMOTO jun-ICHIRO arai 阎锡蕴中国科学院生物物理研究所军事医学科学院微生物流行病研究所 Daikin Industries LTD.Osaka 5918511Japan 中国科学院研究生院北京100039

利用光触媒钛羟基磷灰石网膜 (PTAF)具有吸附和酶催化的特点 ,研究其对SARS病毒的抑制作用 .实验结果表明在紫外照射条件下 ,PTAF膜对SARS冠状病毒的抑制率为 10 0 % .在没有紫外照射的条件下 ,PTAF膜对SARS冠状病毒的抑制率为 99 99% ... 详细信息

利用光触媒钛羟基磷灰石网膜 (PTAF)具有吸附和酶催化的特点 ,研究其对SARS病毒的抑制作用 .实验结果表明在紫外照射条件下 ,PTAF膜对SARS冠状病毒的抑制率为 10 0 % .在没有紫外照射的条件下 ,PTAF膜对SARS冠状病毒的抑制率为 99 99% ,与对照组相比 ,PTAF膜抑制病毒的效率是HAF膜的 10 0 0倍以上 .研究结果提示 ,PTAF在预防SARS冠状病毒及其他病毒性疾病流行方面有潜在的应用价值 .

关键词：光触媒钛羟基磷灰石网膜 SARS冠状病毒光催化作用

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Motor and Nonmotor Components of Event-Brain Potential in Preparation of Motor Response

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Journal of Behavioral and Brain Science 2011年第4期1卷 234-241页

作者： Yasunori Kotani Yoshimi Ohgami jun-ichiro arai Shigeru Kiryu Yusuke Inoue 不详

Stimulus-preceding negativity (SPN), readiness potential (RP), and contingent negative variation (CNV) were recorded to verify the hypothesis that the CNV late wave is the sum of the RP and the SPN. SPN and RP were elicited using a time-estimation task, and the CNV was recorded using a warned reaction-time task. A “virtual CNV” was calculated by superimposing the SPN on the RP. Then the real and virtual CNVs were compared to evaluate the hypothesis. Although an amplitude difference between the real and virtual CNV late waves was observed at the frontal site, the amplitudes at the central and parietal sites were not different between the two. These results suggest that the CNV late wave and the SPN might have a common underlying physiological mechanism in the parietal area, and that these potentials might be related to attentional systems.

关键词： Contingent Negative Variation Stimulus-Preceding Negativity

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Markerless Respiratory Motion Tracking Using Single Depth Camera

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Open Journal of Medical Imaging 2016年第1期6卷 20-31页

作者： Shinobu Kumagai Ryohei Uemura Toru Ishibashi Susumu Nakabayashi Norikazu arai Takenori Kobayashi jun’ichi Kotoku Graduate School of Medical Technology Teikyo University Tokyo Japan Central of Radiology Teikyo University Hospital Tokyo Japan

The aim of this study is to propose a novel system that has an ability to detect intra-fractional motion during radiotherapy treatment in real-time using three-dimensional surface taken by a depth camera, Microsoft Kinect v1. Our approach introduces three new aspects for three-dimensional surface tracking in radiotherapy treatment. The first aspect is a new algorithm for noise reduction of depth values. Ueda’s algorithm was implemented and enabling a fast least square regression of depth values. The second aspect is an application for detection of patient’s motion at multiple points in thracoabdominal regions. The third aspect is an estimation of three-dimensional surface from multiple depth values. For evaluation of noise reduction by Ueda’s algorithm, two respiratory patterns are measured by the Kinect as well as a laser range meter. The resulting cross correlation coefficients between the laser range meter and the Kinect were 0.982 for abdominal respiration and 0.995 for breath holding. Moreover, the mean cross correlation coefficients between the signals of our system and the signals of Anzai with respect to participant’s respiratory motion were 0.90 for thoracic respiration and 0.93 for abdominal respiration, respectively. These results proved that the performance of the developed system was comparable to existing motion monitoring devices. Reconstruction of three-dimensional surface also enabled us to detect the irregular motion and breathing arrest by comparing the averaged depth with predefined threshold values.

关键词： Depth Camera Markerless Motion Tracking Intra-Fractional Motion Three-Dimensional Surface

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定量分析银杏黄酮甙的水解反应条件

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分析化学 2001年第4期29卷 450-452页

作者：陈钧猪股宏新井邦夫东北大学化工系!日本江苏理工大学生物与环境工程学院!镇江212013

提出了定量分析银杏提取物中黄酮甙含量的酸水解反应条件：反应液温度７０～７１℃、反应４ｈ、低速搅拌。

关键词：银杏黄酮水解定量分析

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Clinical practice of transarterial chemoembolization for hepatocellular carcinoma:consensus statement from an international expert panel of International Society of Multidisciplinary Interventional Oncology(ISMIO)

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Hepatobiliary Surgery and Nutrition 2021年第5期10卷 661-671页

作者： Jian Lu Ming Zhao Yasuaki arai Bin-Yan Zhong Hai-Dong Zhu Xiao-Long Qi Thierry de Baere Uei Pua Hyun Ki Yoon David C.Madoff Gao-jun Teng International Society of Multidisciplinary Interventional Oncology(ISMIO) Center of Interventional Radiology&Vascular Surgery Department of RadiologyZhongda HospitalMedical SchoolSoutheast UniversityNanjingChina State Key Laboratory of Oncology in Southern China GuangzhouChina Minimally Invasive&Interventional Department Cancer CenterSun Yat-sen UniversityGuangzhouChina Department of Diagnostic Radiology National Cancer CenterTokyoJapan Department of Interventional Radiology The First Affiliated Hospital of Soochow UniversitySuzhouChina CHESS Frontier Center First Hospital of Lanzhou UniversityLanzhouChina Gustave Roussy-Cancer Campus VillejuifFrance Department of Radiology Tan Tock Seng HospitalNational University of SingaporeSingaporeSingapore Department of Radiology and Research Institute of Radiology University of Ulsan College of MedicineAsan Medical CenterSeoulKorea Department of Radiology and Biomedical Imaging Section of Interventional Radiology and Department of Internal MedicineSection of Medical OncologyYale School of MedicineNew HavenCTUSA 不详

Importance:Transarterial chemoembolization(TACE)has been associated with a wide range of practice variations for hepatocellular carcinoma(HCC)between the East and the *** considerable ambiguity may lead to the heterogeneous quality in treatment and have a negative impact on the role of TACE in the overall multidisciplinary HCC treatment ***:It may be a good start to establish a guideline worldwide to have this consensus from experts who represent east and west,although it does not cover all aspects of *** Review:An international expert panel on TACE is convened to cluster the expert’s opinions and summary a standard *** panel committee consist of leading physicians in TACE on HCC from USA,France,Japan,Singapore,Korea,China,and so *** first-round face-to-face consensus meeting was held during in Nanjing,China in October *** second-round conference for revision of the consensus was held during the Annual Meeting of Chinese College of Interventionalists in August 2020 by a hybrid format of a Webinar and roundtable *** several on-line revisions,the final manuscript was approved by all members of the panel in june ***:The consensus statements were organized into the following categories:patients’selection,performing the procedure,TACE outcomes,repeat TACE,TACE failure/refractory,and TACE-based combination *** and Relevance for Reviews:More and more evidences have showed the better outcomes with strategy of combined TACE with other local therapies such as *** most-recently developing strategy of combined TACE with PD-1/PD-L1 plus tyrosine kinase inhibitor(TKI)agents has shined a light to the HCC patients,especially to those with high risk of tumor recurrence after treatment or TACE failure/refractory.

关键词： Transarterial chemoembolization(TACE) hepatocellular carcinoma(HCC) consensus

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Denoising Projection Data with a Robust Adaptive Bilateral Filter in Low-Count SPECT

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International Journal of Medical Physics, Clinical Engineering and Radiation Oncology 2018年第3期7卷 363-375页

作者： Susumu Nakabayashi Takashi Chikamatsu Takao Okamoto Tatsuro Kaminaga Norikazu arai Shinobu Kumagai Kenshiro Shiraishi Takahide Okamoto Takenori Kobayashi jun’ichi Kotoku Graduate School of Medical Care and Technology Teikyo University Tokyo Japan Central Radiation Division Teikyo University Hospital Tokyo Japan Diagnostic Imaging PET Center Musashimurayama Hospital Tokyo Japan Department of Radiology Teikyo University School of Medicine Tokyo Japan

Low-count SPECT images are well known to be smoothed strongly by a Butterworth filter for statistical noise reduction. Reconstructed images have a low signal-to-noise ratio (SNR) and spatial resolution because of the removal of high-frequency signal components. Using the developed robust adaptive bilateral filter (RABF), which was designed as a pre-stage filter of the Butterworth filter, this study was conducted to improve SNR without degrading the spatial resolution for low-count SPECT imaging. The filter can remove noise while preserving spatial resolution. To evaluate the proposed method, we extracted SNR and spatial resolution in a phantom study. We also conducted paired comparison for visual image quality evaluation in a clinical study. Results show that SNR was increased 1.4 times without degrading the spatial resolution. Visual image quality was improved significantly (p < 0.01) for clinical low-count data. Moreover, the accumulation structure became sharper. A structure embedded in noise emerged. Our method, which denoises without degrading the spatial resolution for low-count SPECT images, is expected to increase the effectiveness of diagnosis for low-dose scanning and short acquisition time scanning.

关键词： Bilateral Filter Denoising SPECT Nuclear Medicine

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Development of Novel Bread by Combining Seaweed Kappaphycus alvarezii from Sri Lanka and Saccharomyces cerevisiae Isolated from Nectarine

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Journal of Agricultural Science and Technology(B) 2019年第5期9卷 339-346页

作者： Noriko Komatsuzaki Sayoko arai Shinobu Fujihara jun Shima Rathnayake Saman Wijesekara MDileepa STde Croos Department of Human Nutrition Seitoku University550 Iwase MatsudoChiba 271-8555Japan Faculty of Agriculture Ryukoku University1-5 YokototaniSeta Oe-ChoOtsuShiga 520-2194Japan Department of Aquaculture and Fisheries Faculty of LivestockFisheries and NutritionWayamba University of Sri LankaGonawila(NWP)60170Sri Lanka

Seaweeds are not used as much for edible use although many kinds of seaweed are collected in Sri *** objective of this study was to develop high quality bread using seaweed,Kappaphycus alvarezii collected from Sri Lanka,and wild yeast,Saccharomyces cerevisiae isolated from nectarine in *** fiber in ***,Japanese sea weeds,Wakame and Aosa contained 48.1,31.7 and 29.1 g/100 g,*** amount of total organic acid increased in garlic bread compared to bread without *** best swelling properties were evident in the bread with ***(0.5%)and *** bread including *** increased the fermentative power of wild yeast while the addition of garlic increased the degree of swelling of the *** the sensory testing,the bread with ***(0.5%)and garlic was favored for the fineness of its texture and had the most preferred *** is possible to develop seaweed bread that is rich in dietary fiber and has excellent flavor by adding a suitable amount of garlic during the fermentation process.

关键词： Kappaphycus alvarezii Saccharomyces cerevisiae organic acids bread making

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