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A new heparin fragment decreases liver ischemia-reperfusion injury

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Hepatobiliary & Pancreatic Diseases International 2022年第2期21卷 190-192页

作者： Enio R Vasques Estela RR Figueira Joel A rocha-filho Cinthia Lanchotte Jorge LS Ximenes Helena B Nader Ivarne LS Tersariol Marcelo A lima Tiago Rodrigues JoséEM Cunha Eleazar Chaib Luiz AC D’Albuquerque Flávio HF Galvão Laboratorio de Investigaçao Medica 37 Departamento de GastroenterologiaHospital das Clinicas HCFMUSPFaculdade de MedicinaUniversidade de São PauloSão Paulo 01246-903Brazil Departamento de Gastroenterologia Hospital das Clinicas HCFMUSPFaculdade de MedicinaUniversidade de São PauloSão PauloBrazil Disciplina de Anestesiologia Hospital das Clinicas HCFMUSPFaculdade de MedicinaUniversidade de São PauloSão PauloBrazil Departamento de Bioquimica Universidade Federal de São PauloSão PauloBrazil Universidade Federal do ABC Santo AndreSão PauloBrazil Serviço de Transplante de Figado e Orgaos do Aparelho Digestivo Departamento de GastroenterologiaHospital das Clinicas HCFMUSPFaculdade de MedicinaUniversidade de São PauloSão PauloBrazil

To the Editor:Ischemia-reperfusion injury following surgery and transplantation can lead to irreversible multiorgan *** calcium overload is associated to cellular death during ischemiareperfusion.A recently discovered heparin fragment (HF),trisulfated disaccharide (TD),that acts on sodium-calcium exchanger(NCX) decreasing intracellular Ca^(2+),showed effectiveness on protecting hepatocytes from ischemia-reperfusion injury [1].

关键词： irreversible heparin injury

Immunoexpression of cyclooxygenase-2 in primary gastric carcinomas and lymph node metastases

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World Journal of Gastroenterology 2012年第8期18卷 778-784页

作者： Paulo RC Almeida Francisco VA Ferreira Cássio C Santos Francisco D rocha-filho Raul RP Feitosa Esther AA Falco Belise K Cavada roberto CP lima-Júnior Ronaldo A Ribeiro Faculty of Medicine Federal University of Ceará Hospital of Cancer Cancer Institute of Ceará

AIM: To evaluate immunoexpression of cyclooxygenase-2 (COX-2) in primary gastric carcinomas and respective lymph node metastases. METHODS: Immunohistochemistry to analyze COX-2 expression was performed on tissue microarray slices obtained from 36 specimens of gastrectomy and satellite lymph nodes from patients with gastric carcinoma. RESULTS: Immunostaining was seen in most cases, and COX-2 expression was higher in lymph node me-tastases than in corresponding primary gastric tumors of intestinal, diffuse and mixed carcinomas, with a statistically signif icant difference in the diffuse histotype (P = 0.0108). CONCLUSION: COX-2 immunoexpression occurs frequently in primary gastric carcinomas, but higher expression of this enzyme is observed in lymph node metastases of the diffuse histotype.

关键词：环氧合酶-2 淋巴结原发性胃癌 COX-2 组织学芯片技术免疫组化

B-1 cells modulate the murine macrophage response to Leishmania major infection

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World Journal of Biological Chemistry 2017年第2期8卷 151-162页

作者： Angelica F Arcanjo Marise P Nunes Elias B Silva-Junior Monique Leandro Juliana Dutra Barbosa da rocha Alexandre Morrot Debora Decote-Ricardo Celio Geraldo Freire-de-lima Instituto de Biofísica Carlos Chagas filho Universidade Federal do Rio de Janeiro Instituto Oswaldo Cruz FIOCRUZ Instituto de Microbiologia Paulo de Góes Universidade Federal do Rio de Janeiro Instituto de Veterinária Universidade Federal Rural do Rio de Janeiro

AIM To investigate the modulatory effect of B-1 cells on murine peritoneal macrophages infected with Leishmania major(L. major) in *** Peritoneal macrophages obtained from BALB/c andBALB/c XID mice were infected with L. major and cultured in the presence or absence of B-1 cells obtained from wild-type BALB/c mice. Intracellular amastigotes were counted, and interleukin-10(IL-10) production was quantified in the cellular supernatants using an enzymelinked immunosorbent assay. The levels of the lipid mediator prostaglandin E2(PGE2) were determined using a PGE2 enzyme immunoassay kit(Cayman Chemical, Ann Arbor, MI), and the number of lipid bodies was quantified in the cytoplasm of infected macrophages in the presence and absence of B-1 cells. Culturing the cells with selective PGE2-neutralizing drugs inhibited PGE2 production and confirmed the role of this lipid mediator in IL-10 production. In contrast, we demonstrated that B-1 cells derived from IL-10 KO mice did not favor the intracellular growth of L. *** We report that B-1 cells promote the growth of L. major amastigotes inside peritoneal murine macrophages. We demonstrated that the modulatory effect was independent of physical contact between the cells, suggesting that soluble factor(s) were released into the cultures. We demonstrated in our co-culture system that B-1 cells trigger IL-10 production by L. major-infected macrophages. Furthermore, the increased secretion of IL-10 was attributed to the presence of the lipid mediator PGE2 in supernatants of L. major-infected macrophages. The presence of B-1 cells also favors the production of lipid bodies by infected macrophages. In contrast, we failed to obtain the same effect on parasite replication inside L. major-infected macrophages when the B-1 cells were isolated from IL-10 knockout mice. CONCLUSION Our results show that elevated levels of PGE2 and IL-10 produced by B-1 cells increase L. major growth, as indicated by the number of parasites

关键词： Leishmania major Macrophages B-1 cells Interleukin-10 Prostaglandin E2 Infection

Subcutaneous Model for the Study of Dengue Virus Infection in Immune Competent Mice

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Journal of Biosciences and Medicines 2018年第5期6卷 97-110页

作者： Beatriz Senra Santos Natalia lima Pessoa Natalia Lucinda Gustavo Cardoso de Oliveira Thais Souza Silva Ketyllen Reis Andrade Ketyllen Reis Andrade Bruno Galvã o filho Marcele Neves rocha Alexandre Vieira Machado Pedro Augusto Alves É rica Alessandra rocha Alves Erna Geessien Kroon Marco Antô nio Campos Instituto René Rachou Funda&amp amp ccedil & amp atilde o Oswaldo Cruz Fiocruz Belo Horizonte Brazil Departamento de Microbiologia Universidade Federal de Minas Gerais Belo Horizonte Brazil Empresa Brasileira de Pesquisa Agropecuá ria Laborat&amp amp oacute rio de Virologia Brasilia Brazil

Various mouse models to study dengue have been described by different authors, some of them using immunodeficient or some using humanized mice. Our group reported previously a deadly murine model, which used the intracranial inoculum of highly virulent Dengue virus (DENV) in immune competent mouse. Here we present a model of immune competent mouse (C57BL/6), infected subcutaneously by the same highly virulent DENV (DENV3 genotype I). In this immunocompetent systemic mice model, the cytokine levels and hematological parameters such as total and differential leukocyte and platelets counts, together with weight loss, were considered important monitoring parameters, allowing a better understanding of the systemic human disease. Mice were inoculated subcutaneously and evaluated by the percentage weight variation as well as the clinical signs. Hematological parameters and cytokines levels were measured and viral titration in brain tissue or serum neutralization was performed to confirm mice infection. The subcutaneously DENV inoculated mice showed weight loss after infection, but they did not show any other clinical signs. The leukocytes and platelets decreased after subcutaneous inoculation. The cytokines TNF alpha and IFN gamma increased after infection in mice. The subcutaneous model provided scope for improved understanding of the dengue pathogenesis, as well as possible mechanism for protection to subsequent mouse infected by intracranial route in mice. This model could be used to study the vertebrate immune response and evaluation of drugs or vaccine against dengue virus.

关键词： Subcutaneous Murine Model Dengue Disease Immune Competent Mouse Cytokines Innate Immune Response

Temporomandibular Disorder in University Students of the Parque das Rosas Campus, Universidade Estácio de SáThat Practice Sports

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Health 2016年第1期8卷 18-23页

作者： Rosineli Paz Cabral Manuel Eduardo Moiolli-Rodrigues Flávia lima Kleinsorgen Motta Francisco Carlos Kleinsorgen de Souza Motta Juliana rocha da Silva Larissa Malheiros Neide Martins Leonardo Trepte André Luiz G. Abrão Adalgisa Maiworm Luiz Carlos Oliveira Raphael Lemgruber Cardoso Adriano Arnóbio Giuseppe Antonio Presta danúbia da Cunha de Sá Caputo Sebastião david Santos filho Éric Heleno F. F. Frederico Mario Bernardo-filho Severo de Paoli Universidade Estácio de Sá—Campus R9—GEPS R9 Rio de Janeiro Brasil Universidade Estácio de Sá—Campus Parque das Rosas Rio de Janeiro Brasil Departamento de Biofísica e Biometria Instituto de Biologia Roberto Alcantara Gomes Universidade do Estado de Rio de Janeiro Rio de Janeiro Brasil Programa de Pós-Graduacao em Fisiopatologia Clínica e Experimental Faculdade de Ciências Médicas Universidade do Estado do Rio de Janeiro Rio de Janeiro Brasil UniFacex—Centro Universitário do Facex Natal Brasil Programa de Pós-Graduacao em Biociências Instituto de Biologia Roberto Alcantara Gomes Universidade do Estado do Rio de Janeiro Rio de Janeiro Brasil

The stomatognathic system (SS) is a functional unit of the body that depends on the balance of several tissues. It consists of various structures, including the temporomandibular joint. Temporomandibular disorder (TMD) can occur due to alterations in the SS. The Fonseca Anamnestic Index (FAI) is a scale used to evaluate and to characterize the TMD Type: no DTM, mild, moderate and severe. The aim is to evaluate the prevalence of signs and symptoms related to TMD in university students of the Parque das Rosas Campus, Universidade Estácio de Sá that practice sports. This investigation was approved (CAAE number 325678413.9.0000.5284). Two hundred eighth students (110 male and 98 female, aged 19 - 35 years) accepted to be in this investigation. All the participants answered a General Questionnaire (GQ) and the FAI. The GQ had questions about the age, sex and the presence of TMD. The FAI was used. A statistical difference (p > 0.05) was not found about the presence of TMD. Among the female, an elevated number of the students with signal or symptoms related to the temporomandibular with statistic significance (p < 0.05) was observed. In the population without TMD, the prevalence of this disorder is higher between male than female. Considering the FAI, among the female students, there is a prevalence of the Mild Type. Considering the evaluation of the type of TMD among the male and female students no difference was found between male and female to the types mild and moderate, however, the prevalence of the severe Type is higher in female than in male with statistical significance. In conclusion, TMD is a relevant clinical condition with an important prevalence among the university students. Moreover, the type of the TMD could be considered due to prevalence of the Type Severe among the women.

关键词： Temporomandibular Disorder University Student Fonseca Anamnestic Index Prevalence