检索结果-南通市图书馆

维普期刊数据库

Inhibition of hepatitis C virus replication by single-stranded RNA structural mimics

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2010年第17期16卷 2100-2108页

作者： robert Smolic martina Smolic John H Andorfer Catherine H Wu robert m Smith George Y Wu Department of medicine Division of Gastroenterology-HepatologyUniversity of Connecticut Health Center263 Farmington Ave.FarmingtonCT 06030-1845United States

AIm: To examine the effect of hepatitis C virus (HCV) structural mimics of regulatory regions of the genome on HCV ***: HCV RNA structural mimics were constructed and tested in a HCV genotype 1b aBB7 replicon,and a Japanese fulminant hepatitis-1 (JFH-1) HCV genotype 2a infection *** sequences were computer-predicted to adopt stem-loop structures identical to the corresponding elements in full-length viral ***7.5 cells bearing the BB7 replicon or infected with JFH-1 virus were transfected with expression vectors generating HCV mimics and *** HCV RNA and protein levels were quantified by real-time polymerase chain reaction and Western blotting,*** evaluate possible antisense effects,complementary RNAs spanning a mimic were ***: In the BB7 genotype 1b replicon system,mimics of the polymerase (NS-5B),X and BA regions inhibited replication by more than 90%,50%,and 60%,*** the JFH-1 genotype 2 infection system,mimics that were only 74% and 46% identical in sequence relative to the corresponding region in JFH-1 inhibited HCV replication by 91.5% and 91.2%,respectively,as effectively as a mimic with complete identity to HCV genotype *** inhibitory effects were confirmed by NS3 protein *** RNA molecules spanning the 74% identical mimic had no significant ***: HCV RNA structural mimics can inhibit HCV RNA replication in replicon and infectious HCV systems and do so independent of close sequence identity with the target.

关键词： Hepatitis C virus Japanese fulminant hepatitis virus Complementarity RNA sequence Hybridization

ASimplified Neuronal model for the Instigation and Propagation of Cortical Spreading Depression

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Applied mathematics and mechanics 2011年第6期3卷 759-773页

作者： Huaxiong Huang robert m.miura Wei Yao Department of mathematics and Statistics York UniversityTorontoOntario M3J1P3Canada Center for Applied mathematics and Statistics New Jersey Institute of TechnologyNewarkNJ 07102USA Department of mathematical Sciences New Jersey Institute of TechnologyNewarkNJ 07102USA Department of mechanics and Engineering Science Fudan UniversityShanghai 200433China

In this paper,we construct a simplified neuronal model that is capable of simulating the instigation of cortical spreading depression(CSD)and propagation of a CSD *** model is a simplification and extension of a single neuron model proposed in the literature for studying the instigation of *** the simplified neuronal model,we construct a network of these simplified *** network model shows that the propagation of a CSD wave occurs naturally after it is instigated electrically or *** the model is simple,the speed of the CSD wave predicted by our model is consistent with experimentally observed ***,our model allows us to investigate the effects of specific ion channels on the spread of a CSD wave.

关键词： Cortical spreading depression neuronal model network numerical simulation

Erosion pattern of artificial gravel deposits

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Sediment Research 2018年第1期33卷 57-67页

作者： Fabian Friedl Volker Weitbrecht robert m.Boes Laboratory of Hydraulics Hydrology and Glaciology (VAW)ETH Zurich

Sediment replenishment with artificial gravel deposits is an option to compensate for sediment deficits in rivers and to improve their ecological conditions. Predicting and quantifying the erosion rate of an artificial gravel deposit is important to successfully perform river restoration projects. Laboratory experiments have been done to investigate the influence of various parameters on the erosion pattern of artificial gravel deposits. In the present paper the effects of deposit geometry, bulk density, grain size distribution, and hydraulic load on the erosion process are described. The temporal evolution of the deposit geometry and the corresponding mean erosion rates were studied. The mean erosion rate increases with deposit height, deposit width, and decreasing grain size. Furthermore, no significant impact of the bulk density was observed. Equations to predict the mean erosion rate are proposed. This investigation helps to determine the design frequency of gravel dumping and deposit volumes for restoration projects.

关键词： Artificial gravel deposits Bed load transport Laboratory experiments Sediment replenishment

Validating a tool to assess eye health knowledge, attitude and practice in Cambodia and Vietnam

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2019年第11期12卷 1767-1774页

作者： Noela Prasad Gail m Ormsby robert P Finger Institute for Health and Sport Victoria UniversityMelbourneVIC 3011Australia VACCHO CollingwoodVIC 3066Australia Professional Studies Faculty of BusinessEducationLaw and ArtsUniversity of Southern QueenslandWest StreetToowoombaQLD 4350Australia Lifestyle Research Centre Avondale College of Higher EducationCooranbongNSW 2265Australia Department of Ophthalmology University of BonnRegina-Pacis-Weg 3Bonn 53113Germany

AIm: To develop an eye health knowledge, attitude and practice(EH-KAP) field-based assessment tool for use in implementing effective eye health care ***: An instrument development and validation study. A Vietnam EH-KAP dataset were used to identify and eliminate redundant questions to develop a standardized tool. Face validity was assessed by the KAP survey team. Internal validity(congruency/criterion) was assessed by comparing descriptive analysis of two datasets(n=531;n=38) collected from the same sampling frame at different time points. Weighted scores were calculated for each construct. Kappa values for test-retest and inter-observer agreement were calculated to check the reliability of responses. The modified version was assessed by analysing the raw and ungrouped data. Responses were weighted and agreement was tested by comparing construct ***: Totally 38 respondents were included in this validation process(mean age 58.5 y). mean scores for knowledge were 9.15(old questionnaire n=531) and 5.05(modified version). For attitude, the scores were 2.23 and 2.42, and for practice the scores were 3.33 and 2.21. Testretest agreement was between 62% to 93%(Kappa 0.24 to 0.86) for the ungrouped raw data, and 55% to 72%(Kappa 0.42 to 0.65) for KAP domain. Inter-observer Kappa value for ungrouped data was 0.37 and 0.45 for the weighted ***: This standardized tool applied at critical time points can assess trends in KAP within the same population and for comparison across groups. If used alongside a Rapid Assessment of Avoidable Blindness(RAAB), this tool provides a comprehensive perspective on eye-health of a population.

关键词： validation knowledge attitude practice eye health knowledge,attitude and practice questionnaire

Stability of Two Conservative,High-Order Fluid-Fluid Coupling methods

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Applied mathematics and mechanics 2019年第6期11卷 1287-1338页

作者： Jeffrey m.Connors robert D.Dolan University of Connecticut Department of MathematicsStorrsCT 06269USA

This paper investigates two methods of coupling fluids across an interface,motivated by air-sea interaction in application *** method is for sequential configurations,where the air code module in invoked over some time interval prior to the sea *** other method is for concurrent setups,in which the air and sea modules run in *** focus is the temporal representation of air-sea *** methods we study conserve moments of the fluxes,with an arbitrary order of accuracy possible in *** step sizes are allowed for the two fluid *** a posteriori stability indicator is defined,which can be computed efficiently on-the-fly over each coupling *** a model of two coupled fluids with natural heat convection,using finite elements in space,we prove the sufficiency of our stability *** certain conditions,we also prove that stability can be enforced by iteration when the coupling interval is small *** particular,for solutions in a certain class,we show that the step size scaling is no worse than O(h)in three dimensions of space,where Oh is a mesh *** is a sharper result than what has been shown previously for related algorithms with finite element *** examples illustrate the behavior of the algorithms under a wide variety of configurations.

关键词： Air-sea atmosphere-ocean fluid-fluid partitioned time stepping conservative coupling

Expression levels of aminopeptidase-N genes in the lightbrown apple moth, Epiphyas postvittana

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2008年第6期15卷 505-512页

作者： robert m. Simpson Joanne Poulton Ngaire P. markwick Horticulture and Food Research Institute Palmerston North Horticulture and Food Research Institute Auckland New Zealand

Five aminopeptidase-N genes （EpAPN1-5） of the tortricid moth Epiphyas postvittana have been isolated from a midgut expressed sequence tag （EST） library. Relative RNA expression of these genes was measured by quantitative reverse transcription polymerase chain reaction using actin as a reference gene. measurements were made on various tissues of fifth instar larvae, over all stages of the life cycle and under differing dietary conditions： different protein sources and levels, and in presence of trypsin and metalloprotease inhibitors. Gene expression for all five genes was the greatest in midgut tissue, but was also detected in the hindgut, fat body and malpighian tubules. EpAPN4 was consistently the highest expressed, with EpAPN3 at about half that level; EpAPN5 was the least expressed. During larval stages expression was high, generally increasing over the instars, after an early peak as neonates or first instars. Expression in other life stages was much lower. males and females showed differing expression in the pupal and adult stages： female expression was higher in the pupa, this reversed in the adult. Gene expression levels and ratios both changed with diet. A natural apple leaf diet depressed levels. Diets with the most impaired amino acid balance induced the greatest change; generally EpAPN1 increased by the greatest proportion. The addition of proteinase inhibitor also increased gene expression, and it was noteworthy that the trypsin inhibitor addition, which has been shown to double aminopeptidase activity, also doubled levels of gene expression.

关键词： aminopeptidase Epiphyas postvittana Q-PCR

Tightening up the structure, lighting up the pathway:application of molecular constraints and light to manipulate protein folding, self-assembly and function

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2014年第12期57卷 1615-1624页

作者： mARKIEWICZ Beatrice N. CULIK robert m. GAI Feng Department of Chemistry University of Pennsylvania Department of Biochemistry and Biophysics University of Pennsylvania

Chemical cross-linking provides an effective avenue to reduce the conformational entropy of polypeptide chains and hence has become a popular method to induce or force structural formation in peptides and ***,other types of molecular constraints,especially photoresponsive linkers and functional groups,have also found increased use in a wide variety of ***,we provide a concise review of using various forms of molecular strategies to constrain proteins,thereby stabilizing their native states,gaining insight into their folding mechanisms,and/or providing a handle to trigger a conformational process of interest with *** applications discussed here cover a wide range of topics,ranging from delineating the details of the protein folding energy landscape to controlling protein assembly and function.

关键词： protein folding aggregation self-assembly cross-linker phototrigger light-activation

维普期刊数据库

Excess dietary Lys reduces feed intake, stimulates jejunal CCK secretion and alters essential and non-essential blood AA profile in pigs.

在线全文

学校读者我要写书评

暂无评论

Can modern Science Answer the Great Questions?

畜牧与生物技术杂志(英文版) 2024年第3期 1336页

作者： maximiliano müller Elout Van Liefferinge Alan Tilbrook robert van Barneveld Eugeni Roura

来源：博看期刊评论

在线全文

博看期刊

学校读者我要写书评

暂无评论

Journal of Earth Science 2022年第5期33卷 1330-1332页

作者： robert E.Criss Anne m.Hofmeister Dept.Earth and Planetary Science Washington UniversitySt.LouisMO63130USA

0 INTRODUCTION Crucial issues facing us are whether current practices prohibit today’s scientists from answering great questions,and how to sufficiently educate the greater population so that scientific methods and f... 详细信息

关键词： Great finding facing

维普期刊数据库