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Effect of Menopause on Gene Expression Profiles of Circu-lating Monocytes: A Pilot in vivo Microarray Study

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Journal of Genetics and Genomics 2007年第11期34卷 974-983页

作者： Dvornyk Volodymyr 刘耀中陆燕沈汇 Lappe Joan M 雷署丰 Recker robert R 邓红文湖南师范大学生命科学学院分子与统计遗传学研究室克瑞屯大学骨质疏松研究中心

Menopause is one of the key physiological events in the female life and can increase the risk for a number of complex autoimmune, neurodegenerative, metabolic, and cardiovascular disorders. Circulating monocytes can differentiate into various cell types and play an important role in tissue morphogenesis and immune response. We studied gene expression profiles of peripheral blood monocytes in healthy pre- and postmenopausal women using Affymetrix Human U133A GeneChip array that contains probes for -14,500 genes. Comparative analyses between the samples showed that 20 genes were up- and 20 were down-regulated. Of these genes, 28 were classified into six major GO categories relevant to such biological processes as the cell proliferation, immune response, cellular metabolism, and the others. The remaining 12 genes have yet unidentified biological functions. Our results support the hypothesis that functional state of circulating monocytes is indeed affected by menopause, and resulting changes may be determined through the genomewide gene expression profiling. Several differentially expressed genes identified in this study may be candidates for further studies of menopause-associated systemic autoimmune, neurodegenerative, and cardiovascular disorders. Our study is only the first attempt in this direction, but it lays a basis for further research.

关键词： menopause monocytes, microarrays differential gene expression

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计划中心——美国底特律新的工程技术奇迹

机电工程技术 1983年第3期 52-53页

作者： robert R. Tripolsky 邓兆恺

所谓“PC”,美国通用汽车公司(简称gm)称之为“计划中心”(Project Centers),福特(Ford)汽车公司叫该系统为“模式管理”,克莱斯勒公司、美国汽车公司(AMC)及美国大众有限公司(VWA)都还没有给它取名。但美国五大汽车公司都在使用计划中... 详细信息

所谓“PC”,美国通用汽车公司(简称gm)称之为“计划中心”(Project Centers),福特(Ford)汽车公司叫该系统为“模式管理”,克莱斯勒公司、美国汽车公司(AMC)及美国大众有限公司(VWA)都还没有给它取名。但美国五大汽车公司都在使用计划中心的方法和原理来发展新的汽车和部件。

关键词：美国通用汽车公司 PC 汽车

Morphology,molecular phylogeny and azaspiracid profile of Azadimium poporum(Dinophyceae) from the Gulf of Mexico

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Morphology,molecular phylogeny and azaspiracid profile of Az...

福建省海洋学会2016年学术年会

作者： Zhaohe Luo Bernd Krock Kenneth Neil Mertens Andrea Michelle Price robert Eugene Turner Nancy N.Rabalais Haifeng Gu College of Science and technology Jinan University Third institute of Oceanography SOA Alfred Wegener institute for Potar and Marine Research Research Unit for Palaeontology Ghent University Department Gengraphy McGiill University Deparfment of Oceanography and Coastal Science Louisiana State University

Azadinium poporum produces a variety of azaspiracids and consists of several ribotypes,but information on its biogeography is limited.A strain of A poponim(gm29) was incubated from a Gulf of Mexico sediment *** gm29 was characterized by a plate pattern of po,cp,x,4′,3a,6″,6C,5S,6′″,2″″,a distinct ventral pore at the junction of po and the first two apical plates,and a lack of an antapical spine,thus fitting the original description of A *** genus Azadinium has not been reported in waters of the United States of America before this *** phylogeny,based on large subunit ribosomal DNA(LSU rDNA) and internal transcribed spacer(ITS) sequences,reveals that strain gm29 is nested within the well-resolved A poponim complex,but forms a sister clade either to ribotype B(ITS) or ribotype C(LSU).It is,therefore,designated as a new ribotype,termed as ribotype *** and ITS sequences similarity among different ribotypes of A poponim ranges from 95.4%to 98.2%.and from97.1%to 99.2%respectively,suggesting that the LSU fragment is a better candidate for molecular *** profiles were analyzed using LC-MS/MSand demonstrate that strain gm29 produces predominantly AZA-2 with an amount of 45 fg/*** results suggest that A popomm has a wide distribution and highlights the risk potential of azaspiracid intoxication in the United States.

关键词： AZA-2 AZA-2 phosphate Biogeography LC-MS/MS LSU rDNA Ribotype D

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慢性肾脏病伴高血压患者的强化血压控制

中华高血压杂志(中英文) 2010年第11期18卷 1005-1005页

作者：张玲玉叶鹏 Appel Lawrence J W Jackson TJr G Tom A Lawrence YA Brad CB George LC William HC Jeanne C Gabriel F Marquetta LG Francis BG Jennifer JH Lee AJ Kenneth AK Joel DK John WL James PL Janice PL Julia BL Michael SM Shaul gm Edgar RN Keith P robert AP Velvie AR Otelio SR StephenG S Miroslaw JT robert DW Xuelei

在观察性研究中发现,血压与终末期肾脏病(endstage renal disease,ESRD)直接相关,并且相互促进。非洲裔美国人中高血压相关的慢性肾脏病和ESRD的患病比例相当高,但极少有试验去检验对非洲裔美国人进行强化血压控制是否能够延缓其慢性肾... 详细信息

在观察性研究中发现,血压与终末期肾脏病(endstage renal disease,ESRD)直接相关,并且相互促进。非洲裔美国人中高血压相关的慢性肾脏病和ESRD的患病比例相当高,但极少有试验去检验对非洲裔美国人进行强化血压控制是否能够延缓其慢性肾脏病进展。方法:入选慢性肾脏病伴高血压的非洲裔美国人患者1094例作为研究对象,随机给予强化或者标准的血压控制治疗。[第一段]

关键词：慢性肾脏病高血压患者血压控制平均血压

各种分化诱导剂对人白血病HL-60细胞中神经节苷脂合成及唾液酸转移酶的影响

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上海医科大学学报 1991年第4期18卷 307-311页

作者：夏霞娟于宽仁上海医科大学基础医学部生物化学教研室美国耶鲁大学医学院神经系

作者采用[^(14)C]-半乳糖研究佛波酯(TPA)、二甲基亚砜(DMSO)、gm3(NeuAe-Gal-Gle-cer)等诱导HL-60细胞分化过程中神经节苷酯(Sg)合成及CMP-Neu Ac:LacCer唾液酸转移酶(ST1)活性的变化。TPA、gm3促进HL-60细胞合成[^(14)C]-Gg,也促进gm3的合成。DMSO抑制HL-60细胞合成[^(14)C]-Gg,但促进gm3的合成。各种制剂处理EL-60细胞后,均能使ST1活性升高,但gm3使ST1活性增加不明显,可能是终产物对ST1的反馈抑制。结果提示Gg(gm3)的生物合成和ST1的活性变化与HL-60细胞的分化增殖有关。

关键词： HL-60细胞神经节苷脂白血病

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硫酸皮肤素寡糖的分离与制备

青岛海洋大学学报（自然科学版） 2001年第6期31卷 849-854页

作者：于广利管华诗 robert J.Linhardt Wang Qun Hyun OK Yang Toshihiko Toida 青岛海洋大学海洋药物与食品研究所青岛266003 美国爱荷华大学药物与天然产物化学系日本千叶大学药物科学学院

利用软骨素酶 ABC (Chondroitinase ABC,EC4 .2 .2 .4 )对硫酸皮肤素 (Dermatan Sul-fate,DS)进行控制降解。对得到的混合寡糖首先采用低压凝胶渗透色谱 (L PGPC)进行分级 ,然后对每一组分再利用强阴离子交换高压液相色谱 (SAX- HPL C)... 详细信息

利用软骨素酶 ABC (Chondroitinase ABC,EC4 .2 .2 .4 )对硫酸皮肤素 (Dermatan Sul-fate,DS)进行控制降解。对得到的混合寡糖首先采用低压凝胶渗透色谱 (L PGPC)进行分级 ,然后对每一组分再利用强阴离子交换高压液相色谱 (SAX- HPL C)进行分离 ,最终制备出聚合度为 2 ,4 ,6 ,8,10 ,12的寡糖纯品。寡糖纯度采用 SAX- HPL C,毛细管电泳 (CE)以及聚丙烯酰胺凝胶电泳(PAGE)进行检验 ,结构采用电喷雾离子化质谱 (ESI- MS)以及核磁共振波谱 (NMR)

关键词：硫酸皮肤素软骨素酶ABC 寡糖 SAX-HLC 分离抗栓剂肝素抗凝剂核磁共振波谱

A pilot double-blind, randomized, placebo-controlled trial of curcumin/bioperine for lung cancer chemoprevention in patients with chronic obstructive pulmonary disease

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Advances in Lung Cancer 2013年第3期2卷 62-69页

作者： Amir Sharafkhaneh J. Jack Lee Diane Liu Ruth Katz Nancy Caraway Cherise Acosta Ignacio I. Wistuba Bharat Aggarwal Burton Dickey Seyed J. Moghaddam Nicola Hanania robert Newman Hanan Abdel-Monem Nga Bich Nguyen Carol J. Farhangfar Waun K. Hong Jonathan M. Kurie Department of Biostatistics The University of Texas MD Anderson Cancer Center Houston USA Department of Experimental Therapeutics The University of Texas MD Anderson Cancer Center Houston USA Department of Medicine Baylor College of Medicine Houston USA Department of Pathology The University of Texas MD Anderson Cancer Center Houston USA Department of Pulmonary Medicine The University of Texas MD Anderson Cancer Center Houston USA Department of Thoracic/Head and Neck Medical Oncology The University of Texas MD Anderson Cancer Center Houston USA Division of Cancer Medicine The University of Texas MD Anderson Cancer Center Houston USA

Chronic obstructive pulmonary disease is an inflammatory condition with increased risk of lung cancer. We hypothesized that curcumin/ bioperine (CB), which has anti-inflammatory effects, may reduce cytological abnormalities in the sputum of patients with COPD. We conducted a 3-month, three-to-one randomized, doubleblind, pilot trial of escalating doses of CB in patients with moderate or worse COPD who were capable of producing sputum. The primary efficacy endpoint was changed in sputum cytology. We also explored changes in fluorescence in situ hybridization (FISH). We obtained sputum samples for cytology and chromosome abnormalities at baseline and each monthly follow-up visit. We enrolled 57 participants, with 35 completing the study. The participants’ mean age (standard deviation [SD]) was 66.6 (8.2) years, and they were mainly male (91.2%), with an average of 63.8 pack-years of smoking history. Also, 42.1% of participants were active smokers and the mean (SD) FEV1 was 37% (13%). At baseline, 13 subjects had moderate or worse dysplasia (22.8%). Subjects with moderate to severe sputum dysplasia had more chromosome abnormalities in epithelial cells and neutrophils, as measured by deletion and aneuploidy in 10q22.3. The changes in sputum cytology and chromosome abnormalities did not differ between the active and placebo arms. CB was well tolerated at the bid doses of 1, 1.5, and 2 gm of curcumin and 5 mg of bioperine, with minor side effects related to the gastrointestinal tract. In this short pilot trial, CB compared to placebo did not alter cytological and chromosomal abnormalities seen in sputum of patients with COPD.

关键词： Lung Cancer Inflammation Nutraceutical Agents COPD Cytological Abnormalities

FAIR Principles:Interpretations and Implementation Considerations

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Data Intelligence 2020年第1期2卷 10-29,293-302,322页

作者： Annika Jacobsen Ricardo de Miranda Azevedo Nick Juty Dominique Batista Simon Coles Ronald Cornet Melanie Courtot Merce Crosas Michel Dumontier Chris T.Evelo Carole Goble Giancarlo Guizzardi Karsten Kryger Hansen Ali Hasnain Kristina Hettne Jaap Heringa Rob W.W.Hooft Melanie Imming Keith G.Jeffery Rajaram Kaliyaperumal Martijn GKersloot Christine R.Kirkpatrick Tobias Kuhn Ignasi Labastida Barbara Magagna PeterMcQuilton Natalie Meyers Annalisa Montesanti Mirjam van Reisen Philippe Rocca-Serra robert Pergl Susanna-Assunta Sansone Luiz Olavo Bonino da Silva Santos Juliane Schneider George Strawn Mark Thompson Andra Waagmeester Tobias Weigel Mark D.Wilkinson Egon L.Willighagen Peter Wittenburg Marco Roos Barend Mons Erik Schultes Leiden University Medical Center Leiden2333 ZAThe Netherlands Institute of Data Science Maastricht UniversityUniversiteitssingel 60Maastricht 6229 ERThe Netherlands Department of Computer Science The University of ManchesterOxford RoadManchester M139PLUK Oxford e-Research Centre Department of Engineering SciencesUniversity of OxfordOxford OX13PJUK School of Chemistry Faculty of Engineering and Physical SciencesUniversity of SouthamptonSO171BJUK Amsterdam UMC University of AmsterdamAmsterdam 1000 GGThe Netherlands European Bioinformatics Institute(EMBL-EBI) HinxtonCambridgeCB101SDUK Harvard University CambridgeMassachusetts 02138USA Department of Bioinformatics–BiGCaT NUTRIMMaastricht UniversityMaastricht 6229 ERThe Netherlands Conceptual and Cognitive Modeling Research Group(CORE) Free University of Bozen-BolzanoBolzano 39100Italy Aalborg University Aalborg DK-9220Denmark Insight Centre for Data Analytics National University of Ireland GalwayH91 TK33Ireland Centre for Digital Scholarship Leiden University LibrariesLeiden2333 ZAThe Netherlands Department of Computer Science Vrije Universiteit AmsterdamDe Boelelaan 11051081 HV AmsterdamThe Netherlands Dutch Techcentre for Life Sciences(DTL) UtrechtThe Netherlands SURF Utrecht 3511 EPThe Netherlands Keith G Jeffery Consultants FaringdonUK Castor EDC Paasheuvelweg 25Wing 5D1105 BPAmsterdamThe Netherlands San Diego Supercomputer Center University of California San DiegoLa JollaCalifornia 92093USA Learning and Research Resources Centre(CRAI) Universitat de Barcelona08007 BarcelonaSpain Environment Agency Austria A-1090 ViennaAustria University of Notre Dame 75004 ParisFrance Health Research Board(HRB) Dublin 2DO2 H638Ireland Liacs Institute of Advanced Computer Science Leiden University2311 GJ LeidenThe Netherlands Czech Technical University in Prague Faculty of Information Technology(FIT CTU)16000 Prague 6Czech Republic GO FAIR International Support&Coordination Office(GFISCO) LeidenThe Netherlands Harvard Catalys

The FAIR principles have been widely cited,endorsed and adopted by a broad range of stakeholders since their publication in *** intention,the 15 FAIR guiding principles do not dictate specific technological implementations,but provide guidance for improving Findability,Accessibility,Interoperability and Reusability of digital *** has likely contributed to the broad adoption of the FAIR principles,because individual stakeholder communities can implement their own FAIR ***,it has also resulted in inconsistent interpretations that carry the risk of leading to incompatible ***,while the FAIR principles are formulated on a high level and may be interpreted and implemented in different ways,for true interoperability we need to support convergence in implementation choices that are widely accessible and(re)-*** introduce the concept of FAIR implementation considerations to assist accelerated global participation and convergence towards accessible,robust,widespread and consistent FAIR *** self-identified stakeholder community may either choose to reuse solutions from existing implementations,or when they spot a gap,accept the challenge to create the needed solution,which,ideally,can be used again by other communities in the ***,we provide interpretations and implementation considerations(choices and challenges)for each FAIR principle.

关键词： FAIR guiding principles FAIR implementation FAIR convergence FAIR communities choices and challenges