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Cidofovir Therapy for Adenovirus Pneumonia in an AIDS Patient on HAART: A Case Report

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World Journal of AIDS 2012年第4期2卷 347-350页

作者： Trong Tony Trinh Quy Ton robert Y. Choi Division of Allergy and Infectious Diseases Department of Medicine University of Washington Seattle USA

Introduction: Adenovirus infections are associated with significant morbidity and mortality among immunocompromised hosts. Adenovirus pneumonia is a rare and often fatal disease in patients with AIDS. Case Report: We report a case of a 28-year-old woman with advanced HIV/AIDS, who developed pneumonia four weeks after initiation of highly active antiretroviral therapy (HAART). Despite empiric antibiotics, the patient developed worsening hypoxemia and progressive pneumonia on chest x-ray. Culture data from a bronchoalveolar lavage (BAL) was negative for bacteria, fungi, pneumocystis jirovecii, but was positive for adenovirus detected by PCR. After transfer to a tertiary care hospital intensive care unit, a repeat BAL confirmed the presence of adenovirus by immunohistochemical staining. Tissue samples sent for histopathology revealed 'smudge cells'. Serum adenovirus viral load was 1.6 × 105 copies/mL. Intravenous cidofovir, 1 mg/kg/day, was initiated and scheduled three times a week. The patient exhibited remarkable improvement and was discharged to home in stable condition after four doses of cidofovir treatment. Discussion: Prior to our case, the few published accounts of HIV patients with adenovirus pneumonia treated with cidofovir have all resulted in death. Our case is distinct from these cases by the lack of concomitant pulmonary infection and the initiation of HAART prior to presentation. Conclusion: To our knowledge, we present the first case of adenovirus pneumonia in a patient with AIDS successfully treated with cidofovir. Our case suggests that limited and low dose cidofovir may be an efficacious approach to treat adenovirus pneumonia among HIV patients, especially those established on HAART.

关键词： Adenovirus Pneumonia AIDS HIV Cidofovir

FTY720,a sphingosine analog,altered placentome histoarchitecture in ewes

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Journal of Animal Science and Biotechnology 2020年第2期11卷 380-392页

作者： Kathrin A.Dunlap Bryan G.White David W.Erikson M.Carey Satterfield Christiane Pfarrer Guoyao Wu Fuller W.Bazer robert C.Burghardt Kayla J.Bayless Greg A.Johnson Department of Animal Science Texas A&M UniversityCollege StationTX 77843USA Okanagan College Salmon Arm Campus Salmon ArmBritish ColumbiaCanada Endocrine Technologies Core Oregon National Primate Research CenterOregon Health&Science UniversityBeavertonORUSA Department of Anatomy University of Veterinary Medicine HannoverBischofsholer Damm 1530173 HannoverGermany Department of Veterinary Integrative Biosciences College of Veterinary Medicine and Biomedical SciencesTexas A&M UniversityCollege StationTX 77843USA Department of Molecular and Cellular Medicine Texas A&M Health Science CenterCollege StationTX 77843USA

Background:The lysosphingolipid,sphingosine-1-phosphate,is a well-described and potent pro-angiogenic ***,as well as the sphingosine phosphorylating enzyme sphingosine kinase 1,are expressed in the placentomes of sheep and the decidua of rodents;however,a function for this signaling pathway during pregnancy has not been *** objective of this study was to investigate whether sphingosine-1-phosphate promoted angiogenesis within the placentomes of pregnant *** were given daily jugular injections of FTY720(2-amino-2[2-(−4-octylphenyl)ethyl]propate-1,3-diol hydrochloride),an S1P ***:FTY720 infusion from days 30 to 60 of pregnancy did not alter maternal organ weights nor total number or mass of placentomes,but did alter placentome *** of caruncular crypts and cotyledonary villi was decreased,as was the relative area of cotyledonary tissue within ***,the percentage of area occupied by cotyledonary villi per unit of placentome was increased,while the thickness of the caruncular capsule was decreased in ewes treated with ***,FTY720 infusion decreased the number and density of blood vessels within caruncular tissue near the placentome capsule where the crypts emerge from the ***,FTY720 infusion decreased asparagine and glutamine in amniotic fluid and methionine in allantoic fluid,and decreased the crown rump length of day 60 ***:While members of the sphingosine-1-phosphate signaling pathway have been characterized within the uteri and placentae of sheep and mice,the present study uses FTY720 to address the influence of S1P signaling on placental *** present evidence that modulation of the S1P signaling pathway results in the alteration of caruncular vasculature,placentome architecture,abundance of amino acids in allantoic and amniotic fluids,and fetal growth during pregnancy in *** marked morphological changes in placentome histoarchitec

关键词： Placentome Pregnancy Sheep Sphingosine1 phosphate(S1P)

Hands-on Workshops as An Efective Means of Learning Advanced Technologies Including Genomics,Proteomics and Bioinformatics

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Genomics, Proteomics & Bioinformatics 2013年第6期11卷 368-377页

作者： Nichole Reisdorph robert Stearman Katerina Kechris Tzu Lip Phang Richard Reisdorph Jessica Prenni David J.Erle Christopher Coldren Kevin Schey Alexey Nesvizhskii Mark Geraci Department of Immunology National Jewish Health Department of Medicine Division of Pulmonary Sciences and Critical Care MedicineUniversity of Colorado School of Medicine Department of Biostatistics and Informatics Colorado School of Public Health Department of Pediatrics National Jewish Health Department of Biochemistry and Molecular Biology Proteomics and Metabolomics FacilityColorado State University Lung Biology Center Department of MedicineUniversity of California Bioinformatics PathgroupNashvilleTN 37027 USA Department of Biochemistry and Mass Spectrometry Research Center Vanderbilt School of Medicine Department of Pathology University of Michigan Medical School

Genomics and proteomics have emerged as key technologies in biomedical research, resulting in a surge of interest in training by investigators keen to incorporate these technologies into their research. At least two types of training can be envisioned in order to produce meaningful results, quality publications and successful grant applications：（1） immediate short-term training workshops and （2） long-term graduate education or visiting scientist programs. We aimed to fill the former need by providing a comprehensive hands-on training course in genomics, proteomics and informatics in a coherent, experimentally-based framework. This was accomplished through a National Heart, Lung, and Blood Institute （NHLBI）-sponsored 10-day Genomics and Proteomics Hands-on Workshop held at National Jewish Health （NJH） and the University of Colorado School of Medicine （UCD）. The course content included comprehensive lectures and laboratories in mass spectrometry and genomics technologies, extensive hands-on experience with instrumentation and software, video demonstrations, optional workshops, online sessions, invited keynote speakers, and local and national guest faculty. Here we describe the detailed curriculum and present the results of short- and long-term evaluations from course attendees. Our educational program consis- tently received positive reviews from participants and had a substantial impact on grant writing and review, manuscript submissions and publications.

关键词： Proteomics Mass spectrometry Genomics Bioinformatics Hands-on Workshops

不同时间点针刺干预对冻融胚胎移植妊娠率的影响

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中国针灸 2022年第9期42卷 987-990页

作者：王雅荣王舢泽 robert Kiltz 赵吉平北京中医药大学东直门医院针灸科北京100700 广州中医药大学第二附属医院/广东省中医院针灸科 Syracuse Fertility Clinic New York 13205

目的:观察不同时间点针刺干预对体外受精-冻融胚胎移植(IVF-FET)妊娠率的影响。方法:将99例拟接受IVF-FET的继发性不孕患者按意愿分为移植日针刺组、移植周期针刺组和非针刺组,每组33例。3组患者均接受常规IVF-FET程序。移植日针刺组于... 详细信息

目的:观察不同时间点针刺干预对体外受精-冻融胚胎移植(IVF-FET)妊娠率的影响。方法:将99例拟接受IVF-FET的继发性不孕患者按意愿分为移植日针刺组、移植周期针刺组和非针刺组,每组33例。3组患者均接受常规IVF-FET程序。移植日针刺组于FET当日移植前1 h与移植后30 min内行针刺治疗;移植周期针刺组于FET前5周(每周治疗1次,共5次)及移植当日行针刺治疗,均穴取子宫、关元、太冲、合谷等。比较3组患者生化妊娠率与临床妊娠率。结果:移植周期针刺组生化妊娠率及临床妊娠率均高于非针刺组(P0.05)。结论:移植周期针刺可有效提高接受IVF-FET的继发性不孕患者妊娠率。

关键词：不孕症冻融胚胎移植针刺临床妊娠率生化妊娠率

Airway Pressure Release Ventilation Improves Oxygenation in a Patient with Pulmonary Hypertension and Abdominal Compartment Syndrome

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Open Journal of Anesthesiology 2013年第1期3卷 14-17页

作者： Arturo G. Torres robert P. Tostenrud Eugenio Lujan Naval Medical Center San Diego San Diego USA

The following case describes the favorable application of airway pressure release ventilation (APRV) in a patient with pulmonary hypertension who developed respiratory failure and abdominal compartment syndrome after abdominal closure from an incarcerated umbilical hernia. A 66-year-old male with past medical history of restrictive lung disease, obstructive sleep apnea and pulmonary hypertension, presented to the operating room for an incarcerated inguinal hernia. After abdominal closure, he gradually developed decreased oxygen saturation and hypotension. APRV was initiated during post operative day 2 after inability to maintain adequate oxygen saturation with resultant hypotension on pressure control ventilation with varying degrees of positive end expiratory pressure and 100% inspired oxygen concentration. The initial set high pressure on APRV was 35 mm Hg. Yet, in lieu of decreasing lung compliance, it peaked at 50 mm Hg. Eventually, inhaled Nitric Oxide was initiated post operative day 3 due to increasing pulmonary arterial pressures. A bedside laparotomy was eventually performed when bladder pressures peaked to 25 mm Hg. APRV gradually and temporally improved the oxygen saturation and decreased the pulmonary arterial pressures with subsequent increase in systemic blood pressures. APRV promoted alveolar recruitment and decreased the shunting associated with abdominal compartment syndrome. Better oxygen saturations lead to increases in blood pressure by decreasing the effects of hypoxic pulmonary vasoconstriction on the right ventricle (RV). In patients with decreasing lung compliance and pulmonary comorbidities, APRV appears safe and allows for improve oxygenation, after failure with conventional modes of ventilation.

关键词： Airway Pressure Release Ventilation Mechanical Ventilation Abdominal Compartment Syndrome Pulmonary Hypertension Positive Pressure Ventilation

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冬至:北半球最短的白昼（英文）

疯狂英语（新读写） 2018年第1期 53-54页

作者： robert Creeley

冬至（Winter Solstice）又名“一阳生”．是中国农历中一个重要的节气．也是中华民族的一个传统节日．冬至俗称“数九”“冬节”“长至节”“亚岁”等．早在2500年前的春秋时代．中国就已经用土圭观测太阳．测定出了冬至．它是二十四... 详细信息

冬至（Winter Solstice）又名“一阳生”．是中国农历中一个重要的节气．也是中华民族的一个传统节日．冬至俗称“数九”“冬节”“长至节”“亚岁”等．早在2500年前的春秋时代．中国就已经用土圭观测太阳．测定出了冬至．它是二十四节气中最早制订出的一个．时间在每年的公历12月21日至23日之间．

关键词：冬至北半球二十四节气中华民族中国公历太阳农历

Evidence for a mouse brain-specific variant of α-tubulin

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Cell Research 1999年第4期9卷 315-325页

作者： KONG LING WEI XIAO YAN DING HIROSHIKITANI robert SHIURBA NAI HE JING( Shanghai Institute of Biochemistry, Chinese Academyof Sciences, 320 Yue Yang Road, Shanghai 200031,China)( Shanghai Institute of Cell Biology, Chinese Academyof Sciences, 320 Yue Ya Shanghai Institute of Biochemistry Chinese Academy of Sciences Shanghai China Shanghai Institute of Cell Biology Chinese Academy of Sciences Shanghai China National Institute of Animal Health Tsukuba Japan Misato Plaheat Mfg. Ltd. Satte-shi Japan

INTRODUCTIONTOinvestigatenestinintermediatefilamentproteinll]expressionduringmousebraindevelopment,weraisedanantibodytoasynthetic20-*******:86-21-... 详细信息

INTRODUCTIONTOinvestigatenestinintermediatefilamentproteinll]expressionduringmousebraindevelopment,weraisedanantibodytoasynthetic20-*******:86-21-...

关键词： Brain-specific a-tubulin Post-translational modification Nestin.

A simple and sensitive LC-MS/MSmethod for simultaneous determination of temsirolimus and its major metabolite in human whole blood

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A simple and sensitive LC-MS/MSmethod for simultaneous deter...

A Validated Liquid Chromatography-Mass Spectrometry Method for the Detection and Quantification of Oxidative Metabolites of 2,2',4,4'-Tetrabromodiphenyl Ether in Rat Hepatic Microsomes

第二届全国治疗药物监测学术年会

作者： Alexander Louie robert Shi Robin K.Kelley Drug Studies Unit Department of Bioengineering & Therapeutic SciencesUniversity of CaliforniaSan FranciscoCA 94143USA. Helen Diller Family Comprehensive Cancer Center University of CaliforniaSan Francisco

A simple,fast and sensitive LC-MS/MS method was developed and validated for the simultaneous determination of the concentrations of temsirolimus and its major metabolite, sirolimus,in human whole *** blood sample(100μL)after adding temsirolimus-d7 and sirolimus-d3 internal standards was precipitated with 0.200 mL of methanol/0.300 M zinc sulfate (70/30,v/v)and then analyzed by a Shimatzu LC system coupled to a Sciex API-5000mass *** chromatographic separation was carried out on a BDS Hypersil C8 column(50 x 3.0 mm,5μrn)at 50℃with a mobile phase composed of methanol/water/formic acid(72/28/0.1) (v/v/v)containing 2.50 mM ammonium *** spectrometric detection was performed using electrospray positive ionization with multiple reactionsmonitoring *** method was validated from 0.250 ngmLto 100 ngmLfor temsirolimus and 0.100 ngmLto 40.0 ngmL for *** lower limits of quantitation were 0.25ngmLfor temsirolimus and 0.1 ngmL for *** precisions(CV%)of intra-day and inter-day for spiked quality control(QC) samples were less than 10.4%and 9.56%,*** accuracies as determined by the relative error for QC samples were less than 12.1%for intra-dayand 7.33%for *** significant matrix effect was *** method has been successfully applied to analyze the clinical pharmacokinetic study *** assay reproducibility was also demonstrated by using incurred samples.

关键词： Temsirolimus sirolimus human blood hepatocellular carcinoma LC-MS/MS

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American Journal of Analytical Chemistry 2011年第3期2卷 352-362页

作者： Sarah Catherine Moffatt Patrick robert Edwards András Szeitz Stelvio Mario Bandiera 不详

In the present study, we developed and validated an analytical method using ultra performance liquid chromatography-mass spectrometry (UPLC/MS) for the quantitative determination of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) metabolism by rat hepatic microsomes. BDE-47 is a brominated flame retardant that was widely used in a variety of consumer products and has subsequently been identified as a ubiquitous environmental contaminant. Hydroxy-bromodiphenyl ethers (OH-BDEs) were isolated from rat hepatic microsomes by liquid-liquid extraction. Chromatographic separation was achieved by UPLC on a C18 column with gradient elution using a mobile phase consisting of methanol and water, each containing 0.1% formic acid, at a flow rate of 0.2 mL/min. Detection and quantification were performed using a mass spectrometer in single ion recording mode with negative electrospray ionization. The UPLC/MS method was validated for linearity, limit of quantification (LOQ), accuracy, precision and recovery. The weighted calibration curves (1/X2) were linear over a concentration range of 5 - 250 nM with LOQ values between 5 nM and 50 nM for the individual OH-BDEs. Intra- and inter- day accuracy (%DEV) and precision (%RSD) values ranged from –11.7% to 9.5% and 5.9% to 16.5%, respectively. Recovery values of 70% to 90% were obtained for all OH-BDEs. The validated method allowed us to successfully analyze metabolite formation following incubation of BDE-47 with hepatic microsomes prepared from phenobarbital-treated rats. Results demonstrate that the UPLC/MS method has sufficient sensitivity and reproducibility to fully characterize the in vitro metabolism of BDE-47 and possibly other PBDEs.

关键词： BDE-47 Hepatic Metabolism Polybrominated Diphenyl Ethers Rat Hepatic Microsomes Ultra Performance Liquid Chromatography-Mass Spectrometry