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A comparative study of conventional and supercritical fluid extraction methods for the recovery of secondary metabolites from Syzygium campanulatum Korth

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第9期17卷 683-691页

作者： Abdul Hakeem memon Mohammad Shahrul Ridzuan HAMIL Madeeha LAGHARI Fahim RITHWAN Salman ZHARI Mohammed Ali ahmed SAEED Zhari ISMAIL Amin Malik Shah Abdul MAJID Department of Pharmaceutical Chemistry School of Pharmaceutical Sciences Universiti Sains Malaysia 11800 Minden Penang Malaysia Center of Lipids Engineering and Applied Research (CLEAR) lbnu Sina Institute for Scientific & Industrial Research (lbnu Sina ISIR) Universiti Teknologi Malaysia 81310 Johor Bahru Johor Malaysia EMAN Research and Testing Laboratory School of Pharmaceutical Sciences Universiti Sains Malaysia 11800 Minden Penang Malaysia

Syzygium campanulatum Korth is a plant, which is a rich source of secondary metabolites （especially flavanones, chalcone, and triterpenoids）. In our present study, three conventional solvent extraction （CSE） techniques and supercritical fluid extraction （SFE） techniques were performed to achieve a maximum recovery of two flavanones, chalcone, and two triterpenoids from S. campanulatum leaves. Furthermore, a Box-Behnken design was constructed for the SFE technique using pressure, temperature, and particle size as independent variables, and yields of crude extract, individual and total secondary metabolites as the dependent variables. In the CSE procedure, twenty extracts were produced using ten different solvents and three techniques （maceration, soxhletion, and reflux）. An enriched extract of five secondary metabolites was collected using n-hexane：methanol （1：1） soxhletion. Using food-grade ethanol as a modifier, the SFE methods produced a higher recovery （25.5%-84.9%） of selected secondary metabo- lites as compared to the CSE techniques （0.92%-66.00%）.

关键词： Syzygium campanulatum Supercritical fluid extraction Conventional solvent extractions Flavanones Chalcone Triterpenoids

Value of Shoulder MRI in Cancer Patients Presenting with Shoulder Related Symptoms

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Journal of Health Science 2019年第5期7卷 320-325页

作者： Bilal Mujtaba Sameh Nassar ahmed Taher Felicia Wang Kundan Rao John E. Madewell rizwan Aslam Kevin McEnery Department of Diagnostic Radiology UT MD Anderson Cancer CenterHoustonTexas 77030USA

Nonspecific shoulder pain is common presentation among elderly patients and patients with a history of cancer. The major concern of shoulder pain in cancer patients is the development of metastases. MRI of the shoulder is the most commonly used imaging modality for assessment of suspected soft tissue pathologies associated with the shoulder pain. In this study, we try to examine the role of shoulder MRI in elderly patients with a history of cancer presenting with nonspecific shoulder complaints for assessment of metastases.

关键词： Shoulder MRI malignancy retrospective analysis

Response of Fodder Maize to Various Levels of Nitrogen and Phosphorus

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American Journal of Plant Sciences 2014年第15期5卷 2323-2329页

作者： Aziz Khan Fazal Munsif Kashif Akhtar Muhammad Zahir Afridi Zahoor Zahoor Ahmad Shah Fahad rizwan Ullah Faheem ahmed Khan Mairaj Din Crop Physiology and Production Center (CPPC) National Key Laboratory of Crop Genetic Improvement MOA Key Laboratory of Crop Eco-Physiology and Cultivation Huazhong Agricultural University Wuhan China Department of Agronomy The University of Agriculture Peshawar Pakistan Key Lab of Vegetable and Plant Diseases Hubei Province Wuhan China Key Laboratory of Animal Genetics Breeding and Reproduction Huazhong Agricultural University Wuhan China Key Laboratory of Horticulture Plant Biology (MOE) Huazhong Agricultural University Wuhan China MOA Key Laboratory of Crop Eco-Physiology and Farming System in the Mid-dle Reaches of the Yangtze River Hauzhong Agricultural University Wuhan China Moecular Biology Lab College of Plant Science and Technology Huazhong Agricultural University Wuhan China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan China

Nitrogen (N) and Phosphorus (P) are of the most important and complex nutrients for the crop plants in particular for grain yield and quality. The field trials were laid out in randomized complete block design having three replications. Research trial plots were located at Agricultural Research Farm of Agricultural University Peshawar (Ameer Mohammad Khan Campus Mardan) during kharif season in 2012 to investigate the response of maize variety (Jalal) to three phosphorus rates (60, 90 and 120 kg·ha-1) and four nitrogen rates (90, 120, 150, 180 kg·ha-1) for agronomical traits. These traits investigated included number of plant per m2 (NP m2), plant height (PH), number of leaves plant-1 (NLP), leaf area plant-1·cm2 (LAP), fresh weight of plants kg·ha-1 (FW) and dry weight of plant kg·ha-1 (DW), were investigated. Results of the study showed that application of N @ 180 and P @ 120 kg·ha-1 significantly increased fodder yield of maize. The linear increase in biomass yield clearly indicated that N was a limiting nutrient factor and that N demand along with P has a positive response. At higher application rates, N fertilizer significantly increased biomass component, improved N uptake with increasing nitrogen use efficiency and decreased its losses to the environment and below plant zone. So this study showed that the phosphorus and nitrogen fertilizers have a positive effect on the fodder yield of maize.

关键词： Fodder Maize Nitrogen Phosphorus

Actin-depolymerizing factor from Eimeria tenella promotes immunogenic function of chicken dendritic cells

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More anxious than depressed:prevalence and correlates in a 15-nation study of anxiety disorders in people with type 2 diabetes mellitus

2021中国畜牧兽医学会兽医寄生虫学分会第二届青年科学家学术论坛

作者： Shakeel ahmed Lakho Muhammad Haseeb Jianmei Huang Zhang Yang Muhammad Waqqas Hasan Muhammad Tahir Aleem Muhammad Ali memon XiaoKai Song RuoFeng Yan Lixin Xu 李祥瑞 College of Veterinary Medicine Nanjing Agricultural University

Dendritic cells play a crucial role in inducing antigen-specific immunity to *** host-parasite interaction,host immune response to the parasite molecules is considered essential for recognizing novel antigens for control ***,in the present study,chicken dendritic cells(DCs)(ChDCs),derived from spleens were used to evaluate their capacity to proliferate and differentiate autologous T lymphocytes in response to actin-depolymerizing factor from Eimeria tenella(EtADF).Immunoblot analysis showed that recombinant EtADF protein(rEtADF) was able to interact with rat anti-rEtADF *** immunofluorescence test confirmed rEtADF binding on ChDCs *** *** revealed that phenotypes for MHCII,CD1.1,CD11 c,CD80,and CD86 were increased in ChDCs after rEtADF ***-PCR results indicated that ChDCs triggered TLR signaling in response to rEtADF,and suppressed Wnt *** levels of CD83,CCL5,and CCR7 in ChDCs were improved following rEtADF *** addition,rEtADF promoted DC-directed T cell proliferation and differentiation of naive T cells into CD3/CD4T cells in DC/T cell co-incubation *** analysis of rEtADF-pulsed ChDCs showed increased levels of IL-12 and IFN-γ,while IL-10 and TGF-β remained ***,rEtADF-treated ChDCs enhanced production of IFN-γ when incubated with T cells,and IL-4 secretion remained *** findings indicted that rEtADF could facilitate the polarization of Th1 immune cells by triggering both host DCs and T *** findings provide useful insights into future work aimed at anticoccidial vaccine strategies.

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General Psychiatry 2019年第4期32卷 197-205页

作者： Santosh K Chaturvedi Shayanth Manche Gowda Helal Uddin ahmed Fahad D Alosaimi Nicola Andreone Alexey Bobrov Viola Bulgari Giuseppe Carra Gianluca Castelnuovo Giovanni de Girolamo Tomasz Gondek Nikola Jovanovic Thummala Kamala Andrzej Ki Nebojsa Lalic Dusica Lecic-Tosevski Fareed Minhas Victoria Mutiso David Ndetei Golam Rabbani Suntibenchakul Somruk Sathyanarayana Srikanta rizwan Taj Umberto Valentini Olivera Vukovic Wolfgang Wolwer Larry Cimino Arie Nouwen Cathy Lloyd Norman Sartorius Department of Psychiatry National Institute of Mental Health and Neuro-SciencesBangaloreIndia Institute of Mental Health BelgradeSerbia Samatvam Endocrinology Diabetes Center and Jnana Sanjeevini Diabetes Hospital and Medical Center BangaloreIndia University of Lower Silesia WroclawPoland Center for Global Mental Health RawalpindiPakistan University of Nairobi NairobiKenya Faculty of Medicine Chulalongkorn UniversityBangkokThailand Pakistan Institute of Medical Sciences IslamabadPakistan Department of Psychiatry and Psychotherapy Medical FacultyUniversity of DusseldorfDusseldorfGermany ProConsult Owings MillsMarylandUSA 不详 Child Adolescent and Family Psychiatry National Institute of Mental HealthDhakaBangladesh Department of Psychiatry King Saud UniversityRiyadhSaudi Arabia IRCCS Ospedale Sacro Cuore Don Calabria NegrarItaly National Research Centre for Psychiatry and Narcology MoscowRussia IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli BresciaItaly University of Milano-Bicocca BicoccaItaly Department of Psychology Catholic UniversityMilanItaly Department of Psychiatry Wroclaw Medical UniversityWroclawPoland

Background Anxiety disorder, one of the highly disabling, prevalent and common mental disorders, is known to be more prevalent in persons with type 2 diabetes mellitus (T2DM) than the general population, and the comorbid presence of anxiety disorders is known to have an impact on the diabetes outcome and the quality of life. However, the information on the type of anxiety disorder and its prevalence in persons with T2DM is limited. Aims To assess the prevalence and correlates of anxiety disorder in people with type 2 diabetes in different countries. Methods People aged 18-65 years with diabetes and treated in outpatient settings were recruited in 15 countries and underwent a psychiatric interview with the Mini-International Neuropsychiatric Interview. Demographic and medical record data were collected. Results A total of 3170 people with type 2 diabetes (56.2% women;with mean (SD) duration of diabetes 10.01 (7.0) years) participated. The overall prevalence of anxiety disorders in type 2 diabetic persons was 18%;however, 2.8% of the study population had more than one type of anxiety disorder. The most prevalent anxiety disorders were generalised anxiety disorder (8.1%) and panic disorder (5.1%). Female gender, presence of diabetic complications, longer duration of diabetes and poorer glycaemic control (HbA1c levels) were significantly associated with comorbid anxiety disorder. A higher prevalence of anxiety disorders was observed in Ukraine, Saudi Arabia and Argentina with a lower prevalence in Bangladesh and India. Conclusions Our international study shows that people with type 2 diabetes have a high prevalence of anxiety disorders, especially women, those with diabetic complications, those with a longer duration of diabetes and poorer glycaemic control. Early identification and appropriate timely care of psychiatric problems of people with type 2 diabetes is warranted.

关键词： type 2 diabetes mellitus anxious depressed

The immunogenic maturation of goat monocyte-derived dendritic cells and upregulation of toll-like receptors by five antigens of Haemonchus contortus in-vitro

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The immunogenic maturation of goat monocyte-derived dendriti...

Glyceraldehyde-3-phosphate dehydrogenase from Eimeria acervulina modulates the functions of chicken dendritic cells to boost Th1 type immune response and stimulates autologous CD4+T cells differentiation in-vitro

2021中国畜牧兽医学会兽医寄生虫学分会第二届青年科学家学术论坛

作者： Muhammad Waqqas Hasan Muhammad Haseeb Muhammad Ehsan Javaid Ali Gadahi Qiangqiang Wang Muhammad Ali memon Muhammad Tahir Aleem Shakeel ahmed Lakho Ruo Feng Yan Li Xin Xu Xiao Kai Song 李祥瑞 College of Veterinary Medicine Nanjing Agricultural University

Previously,it was found that several proteins of Haemonchus contortus were involved in the stimulation of the host immune ***,the information about the selection of superlative antigens with immunogenic efficacies on host DCs is *** the current study,the stimulatory effects of five recombinant proteins(elongation factor-1α,arginine kinase,ES-15,ES-24,and ADP-ribosylation factor 1) of *** on the maturation of goat monocyte-derived dendritic cells(md-DCs) were *** proteins were purified separately in *** expression and incubated with isolated goat peripheral blood mononuclear cells(PBMC).Immunofluorescence assay(IFA) results confirmed the binding of these molecules to the md-DC’s surface as compared to control *** the flow cytometry analysis,recombinant proteins induced md-DC stimulation via the up-regulation of the expression of the costimulatory molecule(CD80) and MHC-Ⅱ.Quantitative RT-PCR data showed a significant increase in the expression of specific genes of the WNT and toll-like receptor(TLR) signaling *** result of ELISA indicated the higher levels of cytokine(IL-10,IL-12,IFN-y,and TNF-α) secretion in the md-DC compared to the negative(pET-32 a His-Tag) and blank(PBS)control *** data gives valuable support in the selection of potential antigens for future studies on the immunomodulation of the host against the infection of ***.

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Glyceraldehyde-3-phosphate dehydrogenase from Eimeria acervu...

2021中国畜牧兽医学会兽医寄生虫学分会第二届青年科学家学术论坛

作者： Shakeel ahmed Lakho Muhammad Haseeb Jianmei Huang Zhang Yang Muhammad Waqqas Hasan Muhammad Tahir Aleem Muhammad Ali-Ul-Husnain Naqvi Muhammad Ali memon XiaoKai Song RuoFeng Yan Lixin Xu 李祥瑞 College of Veterinary Medicine Nanjing Agricultural University

Dendritic cells(DCs) play a pivotal role to amplify antigen-specific immune *** that sensitize T cells via antigen-presentation by DCs could enhance the capacity of host immunity to fight *** this study,we tested the immunogenic profiles of chicken DCs towards Glyceraldehyde-3-phosphate dehydrogenase from Eimeria acervulina(EaGAPDH).Immunoblot analysis showed that recombinant EaGAPDH(rEaGAPDH) protein was successfully recognized by rat sera generated against *** and internalisation of rEaGAPDH by chicken splenic-derived DCs(chSPDCs) was confirmed by immunofluorescence *** cytometry revealed that chSPDCs upregulated MHCII,CD 1.1,CD11 c,CD80,and CD86 cell-surface ***,mRNA expressions of DC maturation biomarkers(CCL5,CCR7,and CD83) and TLR signalling genes(TLR15 and MyD88) were also upregulated whereas those of Wnt signalling were non-significant compared to negative *** treatment induced IL-12 and IFN-γ secretion in chSPDCs but had no effect on IL-10 and TGF-β.Likewise,DC-T cell co-culture promoted IFN-γ secretion and the level of IL-4 was *** of T cells and their differentiation into CD3+/CD4+T cells were triggered in chSPDCs-T cells co-culture *** together,rEaGAPDH could promote Th1 polarization by activating both host DCs and T cells and sheds new light on the role of this important molecule which might contribute to the development of new DCs-based immunotherapeutic strategies against coccidiosis.

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绿色消费在推动高质量发展中的作用

中国环境管理 2020年第1期12卷 24-30页

作者：国合会“绿色转型与可持续社会治理专题政策研究”课题组任勇罗姆松范必张建宇祝宝良周宏春张建平俞海张小丹李继峰 Dr.Lewis AKENJI Dr.Mark SANCTUAR Mrs.Carla MOONEN Dr.Miranda SCHREURS Dr.Mushtaq ahmed memon Dr.Vanessa Timmer 陈刚 Ms.Stefanie CHAN 孟令勃生态环境部环境发展中心北京100029 国合会国办督查室美国环保协会国家信息中心经济预测部国务院发展研究中心商务部国际贸易经济合作研究院区域经济研究中心、西亚非洲所生态环境部环境与经济政策研究中心战略部中环联合认证(北京)有限公司国务院发展研究中心资源与环境研究所能源政策研究室日本地球环境战略研究组织瑞典环境科学研究院荷兰皇家工程师协会慕尼黑工业大学联合国环境署亚太办公室 “一个地球”项目生态环境部环境发展中心国际处斯德哥尔摩环境研究所

当前,中国经济正在从高速增长转向高质量发展,其中消费是拉动经济增长的主要动力,是推动高质量发展的重要动能。从中国整体绿色发展转型进程和状态看,不平衡不协调的问题比较突出,经济维度的绿色转型发展较快较好,社会维度相对滞后。因... 详细信息

当前,中国经济正在从高速增长转向高质量发展,其中消费是拉动经济增长的主要动力,是推动高质量发展的重要动能。从中国整体绿色发展转型进程和状态看,不平衡不协调的问题比较突出,经济维度的绿色转型发展较快较好,社会维度相对滞后。因此,如何在社会生活和消费领域推动绿色转型是中国政府应关注和解决的重大关键问题。国合会2018年主题是“创新引领绿色新时代”,本项目作为“创新与可持续生产和消费”课题下的专题研究,将为国家生态文明建设战略部署下如何推动形成绿色生活方式和消费方式以及提升社会治理体系提供政策决策支持。

关键词：绿色消费高质量发展绿色转型

Sub-percent precision measurement of neutrino oscillation parameters with JUNO

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Chinese Physics C 2022年第12期46卷 28-49页

作者： Angel Abusleme Thomas Adam Shakeel Ahmad rizwan ahmed Sebastiano Aiello Muhammad Akram Abid Aleem Fengpeng An Qi An Giuseppe Andronico Nikolay Anfimov Vito Antonelli Tatiana Antoshkina Burin Asavapibhop João Pedro Athayde Marcondes de André Didier Auguste Weidong Bai Nikita Balashov Wander Baldini Andrea Barresi Davide Basilico Eric Baussan Marco Bellato Antonio Bergnoli Thilo Birkenfeld Sylvie Blin David Blum Simon Blyth Anastasia Bolshakova Mathieu Bongrand Clément Bordereau Dominique Breton Augusto Brigatti Riccardo Brugnera Riccardo Bruno Antonio Budano Jose Busto Ilya Butorov Anatael Cabrera Barbara Caccianiga Hao Cai Xiao Cai Yanke Cai Zhiyan Cai Riccardo Callegari Antonio Cammi Agustin Campeny Chuanya Cao Guofu Cao Jun Cao Rossella Caruso Cédric Cerna Chi Chan Jinfan Chang Yun Chang Guoming Chen Pingping Chen Po-An Chen Shaomin Chen Xurong Chen Yixue Chen Yu Chen Zhiyuan Chen Zikang Chen Jie Cheng Yaping Cheng Yu Chin Cheng Alexey Chetverikov Davide Chiesa Pietro Chimenti Artem Chukanov Gérard Claverie Catia Clementi Barbara Clerbaux Selma Conforti Di Lorenzo Daniele Corti Flavio Dal Corso Olivia Dalager Christophe De La Taille Zhi Deng Ziyan Deng Wilfried Depnering Marco Diaz Xuefeng Ding Yayun Ding Bayu Dirgantara Sergey Dmitrievsky Tadeas Dohnal Dmitry Dolzhikov Georgy Donchenko Jianmeng Dong Evgeny Doroshkevich Marcos Dracos Frédéric Druillole Ran Du Shuxian Du Stefano Dusini Martin Dvorak Timo Enqvist Heike Enzmann Andrea Fabbri Donghua Fan Lei Fan Jian Fang Wenxing Fang Marco Fargetta Dmitry Fedoseev Zhengyong Fei Li-Cheng Feng Qichun Feng Richard Ford Amélie Fournier Haonan Gan Feng Gao Alberto Garfagnini Arsenii Gavrikov Marco Giammarchi Nunzio Giudice Maxim Gonchar Guanghua Gong Hui Gong Yuri Gornushkin Alexandre Göttel Marco Grassi Vasily Gromov Minghao Gu Xiaofei Gu Yu Gu Mengyun Guan Yuduo Guan Nunzio Guardone Cong Guo Jingyuan Guo Wanlei Guo Xinheng Guo Yuhang Guo Paul Hackspacher Caren Hagner Ran Han Yang Han Miao He Wei He Tobias Heinz Patrick Hellmuth Yuekun Heng Rafael Herrera YuenKeung Ho Yerevan Physics Institute YerevanArmenia UniversiéLibre de Bruxelles BrusselsBelgium Universidade Estadual de Londrina LondrinaBrazil Pontificia Universidade Catolica do Rio de Janeiro Rio de JaneiroBrazil Pontificia Universidad Católica de Chile SantiagoChile Universidad Tecnica Federico Santa Maria ValparaisoChile Beijing Institute of Spacecraft Environment Engineering BeijingChina Beijing Normal University BeijingChina China Institute of Atomic Energy BeijingChina Institute of High Energy Physics BeijingChina North China Electric Power University BeijingChina School of Physics Peking UniversityBeijingChina Tsinghua University BeijingChina University of Chinese Academy of Sciences BeijingChina Jilin University ChangchunChina College of Electronic Science and Engineering National University of Defense TechnologyChangshaChina Chongqing University ChongqingChina Dongguan University of Technology DongguanChina Jinan University GuangzhouChina Sun Yat-Sen University GuangzhouChina Harbin Institute of Technology HarbinChina University of Science and Technology of China HefeiChina The Radiochemistry and Nuclear Chemistry Group in University of South China HengyangChina Wuyi University JiangmenChina Shandong University JinanChinaand Key Laboratory of Particle Physics and Particle Irradiation of Ministry of EducationShandong UniversityQingdaoChina Institute of Modern Physics Chinese Academy of SciencesLanzhouChina Nanjing University NanjingChina Guangxi University NanningChina East China University of Science and Technology ShanghaiChina School of Physics and Astronomy Shanghai Jiao Tong UniversityShanghaiChina Tsung-Dao Lee Institute Shanghai Jiao Tong UniversityShanghaiChina Institute of Hydrogeology and Environmental Geology Chinese Academy of Geological SciencesShijiazhuangChina Nankai University TianjinChina Wuhan University WuhanChina Xi'an Jiaotong University Xi'anChina Xiamen University XiamenChina School of Physics and Microelectronics Zhengzhou UniversityZhengzhou

JUNO is a multi-purpose neutrino observatory under construction in the south of *** publication presents new sensitivity estimates for the measurement of the △m_(31)^(2),△m_(21)^(2),sin^(2)θ_(12),and sin^(2)θ_(13) oscillation parameters using reactor antineutrinos,which is one of the primary physics goals of the *** sensitivities are obtained using the best knowledge available to date on the location and overburden of the experimental site,the nuclear reactors in the surrounding area and beyond,the detector response uncertainties,and the reactor antineutrino spectral shape constraints expected from the TAO satellite *** is found that the △m_(21)^(2) and sin^(2)θ_(12) oscillation parameters will be determined to 0.5%precision or better in six years of data *** the same period,the △m_(31)^(2) parameter will be determined to about 0.2%precision for each mass ordering *** new precision represents approximately an order of magnitude improvement over existing constraints for these three parameters.

关键词： neutrino oscillation reactor antineutrino precision measurement JUNO