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Transcriptomic responses to drought stress in the Patagonian southern beech nothofagus alpina

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Ecological Processes 2024年第2期13卷 192-203页

作者： rita Maria Lopez Laphitz María Verónica Arana Santiago Agustín Varela Leandro Aníbal Becker Carolina Soliani María Marta Azpilicueta Paula Marchelli nicolás Bellora Instituto de Diversidad y Evolución Austral (CEnPAT COnICET ) Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB InTA COnICET ) Instituto de Tecnologías nucleares para la Salud (COnICET )

Background Deciphering the genetic architecture of drought tolerance could allow the candidate genes identification responding to water stress. In the Andean Patagonian forest, the genus nothofagus represents an ecologically relevant species to be included in different genomic studies. These studies are scarce in South American ecosystems however represent an important source of genomic data in order to interpret future climate-change environment scenarios of these emblematic forests. Here, we achieved the assemblage of the transcriptome of n. alpina while searching for key genes of activated or suppressed metabolic pathways in response to drought *** De novo transcriptome assembly resulted in 104,030 transcripts. Following confirmation of drought conditions, based on reduction of leaf water potential and stomatal conductance, a differential gene expression analysis resulted in 2720 significantly expressed genes(1601 up-regulated and 1119 down-regulated). Enrichment analysis(over-representation analysis and gene set enrichment analysis) resulted in more than one hundred stress-responsive term ontologies(i.e. biological processes) and pathways. Terms such as response to abscisic acid and pathways such as plant hormone signal transduction or starch and sucrose metabolism were over-represented. Protein–protein interaction assessment resulted in networks with significantly expressed top common hub gene clusters(e.g. planttype cell wall biogenesis among down-regulated or ABA-signalling among up-regulated). These networks evidenced important regulators at gene expression such as transcriptional *** Responses of n. alpina seedlings to drought stress were evidenced by the activation of several genes linked to GO biological processes and KEGG pathways, which were mainly based on over-expression of specific protein kinases, phosphatases, synthases and transcription factors. This suggests an up-regulation of signalling pathways, triggered through pla

关键词： Climate change Differential gene expression Hub genes Patagonia Temperate forest

Ammonia and greenhouse gas distribution in a dairy barn during warm periods

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Frontiers of Agricultural Science and Engineering 2024年第3期11卷 428-441页

作者： Provvidenza rita D’URSO Claudia ARCIDIACOnO Giovanni CASCOnE Department of Agriculture Food and EnvironmentUniversity of CataniaVia S.Sofia n.100Catania 95123Italy

This research aimed to quantify concentrations of ammonia(nH3),carbon dioxide(CO_(2))and methane(CH_(4)),estimate emissions,and analyze the factors influencing them during warm periods in an open dairy barn equipped with two cooling systems in a Mediterranean climate *** distribution within the barn was observed to vary both vertically and horizontally,with the highest gas concentrations observed in the central area of the ***_(3),CH_(4)and CO_(2)ranged in 1.7–7.4,7–18,560–724μg·g^(–1),*** ventilation through openings and the operation of cooling systems induced changes in indoor microclimate conditions,influencing cow behavior and,consequently,gas *** concentrations were the highest at air velocities below 0.5 m·s^(–1).The highest concentration of nH_(3)was observed when the temperature-humidity index(THI)was>72 and≤78;and CO_(2)and CH_(4)concentrations were the highest with THI≥72 and decreased with THI≤***_(3)concentrations when barn management included three daily milkings were higher than those measured when barn management was based on two daily milkings,and lower for CH_(4)and CO_(2).nH_(3)and CH_(4)emissions were the highest during barn cleaning,while the lowest nH_(3)emissions occurred during activity of the cows(i.e.,feeding,walking).

关键词： Ammonia greenhouse gas environmental monitoring cows’behavior barn management housing system climatic parameters

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Syndecans in Alzheimer's disease:pathogenetic mechanisms and potential therapeutic targets

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neural Regeneration Research 2025年第09期 2594-2595页

作者： Carmela rita Balistreri Roberto Monastero Cellular Molecular and Clinical Pathological LaboratoryDepartment of Biomedicine Neuroscience and Advanced Diagnostics (Bi.N.D.) University of Palermo Memory and Parkinson's disease Center Policlinico "Paolo Giaccone" Department of Biomedicine Neuroscience and Advanced Diagnostics (Bi.N.D.)University of Palermo

With increasing age,humans become more susceptible to the onset of neurodegenerative diseases(nDs),and among these,Alzheimer's disease(AD) is the most frequent(nicoletti et al.,2023).nDs are primarily characterized by neuronal loss and atrophy,but also by lesions involving the cerebral and/or cardiovascular system(Balistreri,2021).Lesions such as macroinfa rcts,microinfarcts,hemorrhages,white matter lesions,atherosclerosis,and arteriolosclerosis have also been significantly described in the preclinical stages of cognitive impairment characterizing nDs(Mariani et al.,2007).

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Pro-Environmental Civic Participation in the USA: The Effects of Social Media, Pro-Environmental Lifestyle and Climate Experiences

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American Journal of Climate Change 2024年第1期13卷 31-46页

作者： rita Mano Department of Human Services University of Haifa Haifa Israel

This study addresses the link between social media use and pro-environmental civic participation considering the moderating effect of social media affordances (public realm) on one hand, and lifestyle behaviors and climate change experiences (personal realm) on the other. We combine communication theory and behavioral models and using a sample of USA individuals (n = 7225) based on the American Trends Panel to predict variations in pro-environmental behavior. We show that social networks rather than information are more effective in predicting pro-environmental behavior. Moreover, a pro-environmental lifestyle as well as climate change experiences at the community level increase the likelihood for pro-environmental participation. However, affordances related to socioeconomic variations generate variations to pro-environmental civic participation. We conclude that in order to capture the depth of pro-environmental civic participation, it is necessary to theoretically and empirically bridge between private and public expressions of pro-environmental awareness.

关键词： Pro-Environmental Behavior Socio-Economic Digital Information Digital networks Private Expressions Public Expressions Climate Changes USA

Mixed iridium-nickel oxides supported on antimony-doped tin oxide as highly efficient and stable acidic oxygen evolution catalysts

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Materials Futures 2024年第1期3卷 165-180页

作者： Jonathan Ruiz Esquius Alec P LaGrow Haiyan Jin Zhipeng Yu Ana Araujo rita Marques Adélio Mendes Lifeng Liu Clean Energy Cluster International Iberian Nanotechnology LaboratoryAv.Mestre JoséVeigaBraga 4715-330Portugal Carbon Science and Technology Institute INCAR-CSICFrancisco Pintado Fe 26Oviedo 33011Spain Scientific Imaging Section Okinawa Institute of Science and Technology Graduate UniversityKunigami-gunOkinawa 904-0412Japan Songshan Lake Materials Laboratory Dongguan 523808People’s Republic of China Laboratory for Process Engineering EnvironmentBiotechnology and EnergyFaculty of EngineeringUniversity of PortoRua Dr Roberto FriasPorto 4200-465Portugal Associate Laboratory in Chemical Engineering Faculty of EngineeringUniversity of PortoRua Dr Roberto FriasPorto 4200-465Portugal.

Proton exchange membrane(PEM)water electrolysis represents a promising technology for green hydrogen production,but its widespread deployment is greatly hindered by the indispensable usage of platinum group metal catalysts,especially iridium(Ir)based materials for the energy-demanding oxygen evolution reaction(OER).Herein,we report a new sequential precipitation approach to the synthesis of mixed Ir-nickel(ni)oxy-hydroxide supported on antimony-doped tin oxide(ATO)nanoparticles(IrniyO_(x)/ATO,20 wt.%(Ir+ni),y=0,1,2,and 3),aiming to reduce the utilisation of scarce and precious Ir while maintaining its good acidic OER *** tested in strongly acidic electrolyte(0.1 M HClO_(4)),the optimised Irni1Ox/ATO shows a mass activity of 1.0 mAµgIr^(−1) and a large turnover frequency of 123 s^(−1) at an overpotential of 350 mV,as well as a comparatively small Tafel slope of 50 mV dec^(−1),better than the IrOx/ATO control,particularly with a markedly reduced Ir loading of only 19.7µgIr cm^(−2).Importantly,Irni1O_(x)/ATO also exhibits substantially better catalytic stability than other reference catalysts,able to continuously catalyse acidic OER at 10 mA cm^(−2) for 15 h without obvious *** in-situ synchrotron-based x-ray absorption spectroscopy confirmed that the Ir^(3+)/Ir^(4+)species are the active sites for the acidic ***,the performance of Irni1Ox/ATO was also preliminarily evaluated in a membrane electrode assembly,which shows better activity and stability than other reference *** Irni1Ox/ATO reported in this work is a promising alternative to commercial IrO_(2) based catalysts for PEM electrolysis.

关键词： mixed oxides oxygen evolution reaction polymer electrolyte membrane antimony doped tin oxide membrane electrode assembly

Methionine sources and genotype affect embryonic intestinal development,antioxidants,tight junctions,and growth-related gene expression in chickens

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Animal nutrition 2024年第1期16卷 218-230页

作者： James K.Lugata Sawadi F.ndunguru Gebrehaweria K.Reda Xénia E.Ozsváth Eszter Angyal Levente Czeglédi Gabriella Gulyás Renáta Knop János Oláh Zoltán Mészár rita Varga Brigitta Csernus Csaba Szabó Department of Animal nutrition and Physiology Faculty of Agriculture and Food Sciences and Environmental ManagementUniversity of DebrecenDebrecenHungary Department of Animal Husbandry Faculty of Agriculture and Food Sciences and Environmental ManagementUniversity of DebrecenDebrecenHungary Faculty of Agriculture and Food Sciences and Environmental Management Doctoral School of Animal ScienceUniversity of DebrecenDebrecenHungary Institutes for Agricultural Research and Educational Farm University of DebrecenDebrecenHungary Department of Anatomy Histology and EmbryologyFaculty of MedicineUniversity of DebrecenDebrecenHungary Department of Evolutionary Zoology and Human Biology Faculty of Science and TechnologyUniversity of DebrecenDebrecenHungary

Methionine(Met)is an essential and first limiting amino acid in the poultry diet that plays a significant role in chicken embryonic development and *** present study examined the effect of in ovo injection of DL-Met and L-Met sources and genotypes on chicken embryonic-intestinal development and *** eggs of the two genotypes,TETRA-SL layer hybrid(TSL)—commercial layer hybrid and Hungarian Partridge colored hen breed(HPC)—a native genotype,were randomly distributed into four treatments for each *** treatment groups include the following:1)control non-injected eggs(noIn);2)saline-injected(SaIn);3)DL-Met injected(DLM);and 4)L-Met injected(LM).The in ovo injection was carried out on 17.5 d of embryonic development;after hatching,eight chicks per group were sacrificed,and the jejunum was extracted for *** results showed that both DLM and LM groups had enhanced intestinal development as evidenced by increased villus width,villus height,and villus area(Pnificantly reduced crypt depth,glutathione(GSH)content,glutathione S-transferase 3 alpha(GST3),occludin(OCLn)gene expression and increased villus height to crypt depth ratio in the TSL genotype than the LM group(Pnotype has overexpressed insulin-like growth factor 1(IGF1)gene,tricellulin(MD2),occludin(OCLn),superoxide dismutase 1(SOD1),and GST3 genes than the TSL genotype(Pn conclusion,these findings showed that in ovo injection of Met enhanced intestinal development,and function,with genotypes responding differently under normal *** also influenced the expression of intestinal antioxidants,tight junction,and growth-related genes.

关键词： DL-Methionine L-Methionine In ovo injection Intestinal development Poultry

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Reduction Discoloration of Reactive Dyed Cotton Waste and Chemical Recycling via Ionic Liquid

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Journal of Renewable Materials 2024年第9期12卷 1557-1571页

作者： Aline Ferreira Knihs Larissa Klen Aragão Miguel Angelo Granato Andrea Cristiane Krause Bierhalz rita de Cassia Siqueira Curto Valle Department of Textile Engineering Federal University of Santa CatarinaBlumenau89036-256Brazil

The textile industry generates large volumes of waste throughout its production *** of this waste is colored,therefore,discoloration is an important step toward recycling and reusing this *** study focused on the chemical reductive discoloration of textile waste composed of cotton dyed with reactive *** experimental design demonstrated the significant influence of the concentration of reducing agent and time of reaction on the degree of whiteness of the cotton *** concentration of the alkaline agent was not significant in the *** optimization of the reaction conditions lead to Berger degree of 50.5±*** discolored cotton was chemically recycled through dissolution in ionic liquid 1-ethyl-3-methylimidazolium chloride and regeneration in film form in *** microstructure of the regenerated cellulose films was evaluated by Scanning Electron Microscopy(SEM)indicating complete dissolution and uniform *** discoloration process reduced the polymerization degree and crystallinity index of the cotton fibers but retained the cellulose I *** dissolution and cellulose regeneration process results in transparent films with an amorphous *** thermal behavior,evaluated by thermogravimetric analysis,indicated that residues and regenerated film presented a main decomposition *** maximum decomposition rate temperature of the regenerated films was approximately 40℃lower than the cotton fibers,which correlates well with the reduction in polymerization degree and amorphous *** general,the study demonstrated that textile cotton waste dyed with reactive dyes can be chemically discolored to form transparent and amorphous films,contributing to the development of sustainable strategies for the textile industry.

关键词： Reactive dyed cotton waste reactive discoloration ionic liquid dissolution and regeneration recycling and reusing

The biosynthetic pathway of the hallucinogen mescaline and its heterologous reconstruction

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Molecular Plant 2024年第7期17卷 1129-1150页

作者： Paula Berman Luis Alejandro de Haro Ana-rita Cavaco Sayantan Panda Younghui Dong nikolay Kuzmich Gabriel Lichtenstein Yoav Peleg Hila Harat Adam Jozwiaki Jianghua Cai Uwe Heinig Sagit Meir Ilana Rogachev Asaph Aharoni Department of Plant and Environmental Sciences Weizmann Institute of ScienceRehovot 7610001Israel Life Sciences Core Facilities Weizmann Institute of ScienceRehovot 7610001Israel The Maurice and Vivienne Wohl Institute for Drug Discovery Weizmann Institute of ScienceRehovot 7610001Israel Present address:Department of Cell and Metabolic Biology Leibniz Institute of Plant BiochemistryWeinberg 306120 Halle(Saale)Germany Department of Molecular&Cellular Pharmacology University of Miami Miler School of MedicineMiamiFL 33136USA Department of Botany and Plant Sciences University of California RiversideRiversideCA 92521USA Laboratory of Plant Hormones and Development Regulation of Chongqing School of Life SciencesChongqing UniversityChongqing 401331China

Mescaline,among the earliest identified natural hallucinogens,holds great potential in psychotherapy ***,despite the existence of a postulated biosynthetic pathway for more than half a century,the specific enzymes involved in this process are yet to be *** this study,we investigated the cactus Lophophora williamsii(Peyote),the largest known natural producer of the phenethylamine *** employed a multi-faceted approach,combining de novo whole-genome and transcriptome sequencing with comprehensive chemical profiling,enzymatic assays,molecular modeling,and pathway engineering for pathway *** identified four groups of enzymes responsible for the six catalytic steps in the mescaline biosynthetic pathway,and an n-methyltransferase enzyme that n-methylates all phenethylamine intermediates,likely modulating mescaline levels in ***,we reconstructed the mescaline biosynthetic pathway in both nicotiana benthamiana plants and yeast cells,providing novel insights into several challenges hindering complete heterologous mescaline *** together,our study opens up avenues for exploration of sustainable production approaches and responsible utilization of mescaline,safeguarding this valuable natural resource for future generations.

关键词： mescaline Lophophora williamsi Peyote biosynthetic pathway de novo genome sequencing mo-lecular modeling

Liquid crystal immunosensors for the selective detection of Escherichia coli with a fast analysis tool

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Photonics Research 2024年第7期12卷 1564-1573页

作者： SAnDRO C.OLIVEIRA MARIA S.SOARES BÁRBARA V.GOnÇALVES AnDREIA C.M.RODRIGUES AMADEU M.V.M.SOARES rita G.SOBRAL nUnO F.SAnTOS JAn nEDOMA PEDRO L.ALMEIDA CARLOS MARQUES CICECO-Aveiro Institute of Materials Physics DepartmentUniversity of Aveiro3810-193 AveiroPortugal i3n Physics DepartmentUniversity of Aveiro3810-193 AveiroPortugal Associate Laboratory i4HB-Institute for Health and Bioeconomy School of Sciences and TechnologyNOVA University of Lisbon1099-085 LisbonPortugal CESAM-Centre for Environmental and Marine Studies and Department of Biology University of Aveiro3810-193 AveiroPortugal Department of Telecommunications VSB-Technical University of OstravaOstrava 70800Czech Republic i3n-CEnIMAT School of Sciences and TechnologyNOVA University of Lisbon1099-085 LisbonPortugal UnIRE ISELPolytechnic Institute of Lisbon1549-020 LisbonPortugal Department of Physics VSB-Technical University of OstravaOstrava 70800Czech Republic

The consumption of contaminated food may cause serious illnesses,and traditional methods to detect Escherichia coli are still associated with long waiting times and high costs given the necessity to transport samples to specialized *** is a need to develop new technologies that allow cheap,fast,and direct monitoring at the site of ***,in this work,we developed optical immunosensors for the selective detection of ***,based on liquid crystal technology,whose molecules can align in different manners depending on the boundary conditions (such as substrates) as well as the environment that they *** glass substrate was functionalized with *** antibody using cysteamine as an intermediate,and a vertical alignment was imposed on the liquid crystal molecules by using DMOAP during *** presence of bacteria disrupts the alignment of the liquid crystal molecules,changing the intensity of light emerging between cross polarizers,measured using a polarized optical microscope and a *** was possible to detect *** in suspensions in the concentration range from 2.8 cells/mL to 2.8×10^(9) cells∕*** was also evaluated,and the sensors were used to analyze contaminated water samples.A prototype was developed to allow faster,in-situ,and easier analysis avoiding bulky instruments.

关键词： crystal alignment analysis

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