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Efficacy assessment of peracetic acid in the removal of synthetic 17α-ethinyl estradiol contraceptive hormone in wastewater

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Journal of Environmental Sciences 2020年第3期32卷 1-8页

作者： rita Maurício Flávia Semedo rita dias Joao P.Noronha Leonor Amaral Michiel A.Daam Antonio P.Mano Mário S.Diniz CENSE Center for Environmental and Sustainability ResearchNOVA School of Science and TechnologyNOVA University LisbonQuinta da Torre2829-516 CaparicaPortugal Department of Environmental Sciences and Engineering NOVA School of Science and TechnologyNOVA University LisbonQuinta da Torre2829-516 CaparicaPortugal REQUIMTE/FCT NOVA School of Science and TechnologyNOVA University LisbonQuinta da Torre2829-516 CaparicaPortugal Biotox Lab UCIBIODepartment of ChemistryNOVA School of Science and TechnologyNOVA University LisbonQuinta da Torre2829-516 CaparicaPortugal

Increasing concerns have been raised on endocrine disrupting chemicals like the sex hormone 17 a-ethinylestradiol(EE2),the more since traditional wastewater(WW) treatments appear to be ineffective for their *** efficacy of the relatively novel disinfectant peracetic acid(PAA) in EE2 removal was evaluated,as well as its potential effects on WW quality *** treatments tested for EE2 removal were also evaluated in terms of toxicity,through the determination of biochemical responses(antioxidant enzymes,lipid peroxidation and vitellogenin induction) using zebrafish(Danio rerio) as a biological *** contact times less than 20 min appeared insufficient regardless of the PAA dose tested,but a 100% EE2 removal was attained at a PAA concentration of 15 mg/L with a contact time of20 *** suspended solids,chemical oxygen demand and pH in PAA treatments remained well within levels set in European legislation for WW ***2 induced signific ant increased vitellogenin(VTG) levels in both female and male fish,indic ating increased estrogenic activity,especially in males suggesting an endocrine disruption *** the addition of PAA(15 mg/L),however,VTG levels in both sexes returned to control *** this PAA treatment showed increased levels of the antioxidant enzyme catalase,the lipid peroxidation levels were similar or even lower than in *** the results suggest that the use of PAA appears a promising way forward as a less toxic alternative to chlorine disinfection with high efficiency in the removal of EDC like EE2.

关键词： EE2 Wastewater treatment Zebrafish Biomarkers

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^(222)Rn calibration procedure for water analyses by liquid scintillation counting

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辐射探测技术与方法(英文) 2023年第2期7卷 311-319页

作者： Ana rita Gomes Sérgio dias Anabela Mourato Joao Abrantes Mário Reis Laboratório de Protecao e Seguranca Radiológica Instituto Superior TécnicoUniversidade de LisboaEstrada Nacional 10km 13972695‑066 Bobadela LRSPortugal Laboratórios de Massa e Massa Volúmica de Sólidos Instituto Portugues da QualidadeRua António Giao22829‑513 CaparicaPortugal Centro de Ciências e Tecnologias Nucleares Instituto Superior TécnicoUniversidade de LisboaEstrada Nacional 10km 13972695‑066 Bobadela LRSPortugal

Purpose Among the several methods to measure ^(222)Rn in water,the liquid scintillation counting(LSC)technique is one of the most sensitive and widely used when analysing non-saline waters by liquid extraction,providing excellent accuracy,precision and low-level detection *** using the liquid scintillation counter Tri-Carb 3170 TR/SL(Perkin-Elmer)it is not possible to make an automatic determination of the alpha/beta discrimination parameter,being necessary to evaluate the pulse decay discriminator(PDD)value manually to the ^(222)Rn *** this work,we describe the steps to perform the calibration of that type of *** The method consists in the preparation of standard samples from a ^(226)Ra certified standard solution and the adjustment of the PDD value by hand until the percentage of alpha events reaches a plateau of a near-constant fraction of total *** process consists in taking several measures between PDD 80 and 200 with 15 min counting time *** the plateau,the alpha/beta ratio was used to determine the plateau final range as well as the optimum PDD *** that,the calibration standards and blanks were measured and the region of interest(ROI)was established by finding the maximum value for the figure of merit(FOM)in the upper and lower *** The optimal PDD value of 148 was established by the alpha/beta ratio,and after that,it was possible to measure ^(226)Ra standards to perform the *** efficiency of(9.758±0.035)×1^(0-1) was *** routine measurements,a detection limit of about 0.50 Bq L^(-1) is achieved for a 120 min counting *** evaluate the uncertainty budget,the uncertainties of the net count rate,the efficiency,the sample amount and the decay correction factor were *** A method was developed for the calibration of LSC Tri-Carb 3170 TR/SL with the optimization of the PDD using a ^(226)Ra standard *** acquisition to obtain the plot of the variation

关键词： Radon Waters Pulse decay discriminator Liquid scintillation counting Calibration

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Amprolium exposure alters mice behavior and metabolism in vivo

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Animal Models and Experimental Medicine 2018年第4期1卷 272-281页

作者： Juliana Oliveira Moraes Samara dias Cardoso Rodrigues Leidiano Martins Pereira rita de Cássia Noronha Medeiros Clarissa Amorim Silva de Cordova Fabiano Mendes de Cordova Programa de Pós-Gradua??o em Sanidade Animal e Saúde Pública nos Trópicos Universidade Federal do Tocantins Curso de Medicina Veterinária Universidade Federal do Tocantins Curso de Medicina Universidade Federal do Tocantins

Background: Thiamine deficiency(TD) models have been developed, mainly using the thiamine analog pyrithiamine. Other analogs have not been used in rodents. We aimed to evaluate the effects and mechanisms of intraperitoneal(ip) amprolium-induced TD in mice. We also evaluated the associated pathogenesis using antioxidant and anti-inflammatory compounds(Trolox, dimethyl sulfoxide).Methods: Male mice were separated into two groups, one receiving a standard diet(control animals), and the other a TD diet(deficient groups) for 20 days. Control mice were further subdivided into three groups receiving daily ip injections of saline(NaCl0.9%; Cont group), Tolox(Tr group) or dimethyl sulfoxide(DMSO; Dmso group). The three TD groups received amprolium(Amp group), amprolium and Trolox(Amp+Tr group), or amprolium and DMSO(Amp+Dmso group). The animals were subjected to behavioral tests and then euthanized. The brain and viscera were ***: Amprolium exposure induced weight loss with hyporexia, reduced the behavioral parameters(locomotion, exploratory activity, and motor coordination), and induced changes in the brain(lower cortical cell viability) and liver(steatosis). Trolox co-treatment partially improved these conditions, but to a lesser extent than ***: Amprolium-induced TD may be an interesting model, allowing the deficiency to develop more slowly and to a lesser extent. Amprolium exposure also seems to involve oxidative stress and inflammation, suggested as the main mechanisms of cell dysfunction in TD.

关键词： brain dimethyl sulfoxide thiamine deficiency Trolox

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Opportunistic Infections in Renal Transplantation <br/>—A Case Series

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Open Journal of Pathology 2017年第1期7卷 13-19页

作者： rita Sampaio José Palla Garcia Leonídio dias La Salete Martins José Ramón Vizcaíno Department of Pathology Hospital de Santo António Centro Hospitalar do Porto Porto Portugal Department of Nephrology Hospital de Santo António Centro Hospitalar do Porto Porto Portugal

Background: Porto’s Hospital Centre is one of the most active Portuguese hospitals in renal transplantation (performed since 1983). Although increasingly rare, opportunistic infections in transplanted patients are associated with high mortality rate in kidney transplantation and remain a major diagnostic challenge. Methods: We investigated 2041 cases of hospital admissions (from 2004 to 2012), any time after kidney transplantation. We described the infection location, the diagnostic techniques used and the mortality after the infection. Results: We found 82 cases of opportunistic infection caused by Herpes virus (Zoster and simplex), Cytomegalovirus, Polyomavirus, Aspergilus, Alternaria, Mucorales, Candida, Mycobacterium tuberculosis, Cryptococcus and Pneumocystis. Conclusions: In this article we highlight the important role of histology/cytology in the diagnostic process of these infections. In many cases prompt diagnosis and treatment are necessary to avoid life-threatening complications and may greatly improve prognosis.

关键词： Transplant Pathology Kidney Opportunistic Infections

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The Biotechnological and Seafood Potential of Stichopus regalis

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Advances in Bioscience and Biotechnology 2015年第3期6卷 194-204页

作者： rita Santos Simaura dias Susete Pinteus Joana Silva Celso Alves Carla Tecelao Ana Pombo Rui Pedrosa Department of Geography Environment and Earth Sciences(GEES)Faculty of ScienceUniversity of HullHullUK MARE—Marine and Environmental Sciences Centre ESTMPolytechnic Institute of LeiriaPenichePortugal Linking Landscape EnvironmentAgriculture and Food Research Unit(LEAF)Instituto Superior de AgronomiaUniversity of LisbonTapada da AjudaLisbonPortugal

Sea cucumbers are highly used not only for the production of the bêche-de-mer, but also recent studies have been showing the high potential of these marine invertebrates on the pharmaceutical industry, as they are rich in bioactive compounds with important functions. Stichopus regalis extracts (1 mg·mL-1) were used in antioxidant, antimicrobial and antitumor assays. The lipid profile was also evaluated. No significant antioxidant activity was detected in both methanolic and dichloromethane extracts. The methanolic fraction showed the highest antimicrobial potential against Candida albicans with an IC50 of 475.4 μg·mL-1. In the antitumor assays, the dichloromethane fraction showed a high potential for both cell lines, as revealed by the MTT method. The total fat content was 3.63% ± 0.11% and the fatty acid profile revealed the highest amount in C16:0 (9.43% ± 0.77%), C18:0 (12.43% ± 0.83%), C18:1 ω7 (5.63% ± 0.33%), EPA (12.49% ± 0.15%), DHA (7.35% ± 0.02%), ARA (19.29% ± 0.14%) and a ω3/ω6 ratio of 1.078. These findings led us to suggest the potential use of S. regalis as a new source of bioactive compounds with pharmacological potential and its nutritional benefits for human health.

关键词： Sea Cucumber Antimicrobial Activity Antitumor Activity Marine Natural Compounds Fatty Acid Profile

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Genitourinary Bacillus Calmette-Guerin Infection after BCG Intravesical Administration in a Patient under Hemodialysis—What Can We Do Better?

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Advances in Infectious Diseases 2022年第3期12卷 563-567页

作者： Lúcia Ribeiro dias Catarina Almeida Fernando Nogueira rita Soares Sofia Garcês Soares Maria Lima Costa Maria Teresa Boncoraglio Filipa Ceia Infectious Diseases Department University Hospital Center of S&#227 o Joã o Porto Portugal Internal Medicine Department Hospital Center of Tondela-Viseu Viseu Portugal Internal Medicine Department University Hospital Center of S&#227 o Joã o Porto Portugal Internal Medicine Department Tamega e Sousa Hospital Center Penafiel Portugal

A 74-year-old man with terminal chronic kidney disease, under hemodialysis and with residual diuresis, was admitted due to myalgia, arthralgia, fever and pyuria in the previous 10 days. The patient had a recent diagnosis of high-grade non-invasive bladder cancer and was doing weekly BCG intravesical administrations. The symptoms started three days before the fifth administration. He had done cefixime as an outpatient and started piperacillin-tazobactam on hospital admission, but the fever persisted, and there was no bacterial isolation in urine or blood culture. On the tenth and seventeenth day after the last BCG intravesical administration Mycobacterium bovis was still isolated in the urine culture. The diagnosis of BCGitis was made and treatment was started, with a good response. Forty days after the last administration and under treatment, the culture remained positive for Mycobacterium bovis in the urine. We raise the question about the safety of BCG administration in patients with residual diuresis.

关键词： BCGitis Mycobacterium Bovis Hemodialysis Non-Tuberculosis Mycobacterium Bladder Cancer

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The Ethics and the Meaning of Life among College Students

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International Journal of Clinical Medicine 2018年第4期9卷 221-233页

作者： Maria Helena de Agrela Goncalves Jardim Geraldo Bezerra da Silva Junior Marcia Lucia Sousa dias Alves Bruna Raquel Figueira Ornelas de Gouveia rita Baptista Silva School of Health University of MadeiraFunchalPortugal Visiting Researcher at UNIFOR by Capes/Funcap FortalezaBrazil Health Sciences Center University of FortalezaFortalezaBrazil Hospital of Dr.Nelio Mendonca MadeiraPortugal Abel Salazar Biomedical Sciences Institute University of PortoPortoPortugal 6School of Nursing of Sao Jose de ClunnyFunchalPortugal Nursing Sciences University of PortoPortoPortugal

The concern of the ethics and the meaning of life in young adults became universal and are present in all contexts of human life. Such universality is due to the development of modern rationality, to establish a relationship between the intrinsic theoretical dimensions (scientific) and practical dimensions (ethical), originated that are present in the own array of knowledge. This descriptive cross-sectional study, aims to identify the values of the ethical dimension and meaning of life defended by the college students of the Universities of Madeira, Portugal and Fortaleza, Brazil. The sample consists of 605 University students (Madeira = 225 and Fortaleza = 380), with an average age of 21 years and a standard deviation of 2. We used the questionnaire on values (adapted from the European Values Survey). Most of the young people referred to have a religion (60% wood and 78.4% stronghold), being the most Catholic, however, present in your frequency disinterest and, yet, in Brazil there is a greater diversity of religions. Supporting the intervention of religious institutions in situations of everyday life is accepted mostly by Brazilians, with the exception of politics (35.5%). On the other hand, the university students of the University of Madeira understand that Religious Institutions should only give their opinion on racial discrimination (76.6%). Believe in God (90.8% and 55.1% Stronghold) and Soul (80.3% and 66.4% Stronghold) are beliefs more referred to by college students. These results are intended to contribute in the field of research on ethical values and meaning of life in young people. This study contributes to future comparative research among university students and to developing strategic programs in education and health, increasing the success and excellence in the formation of a full citizenship, preventing the fanaticisms and the social discrimination that today devastate the collective globally.

关键词： Values Ethics Meaning of Life Young Adults College Students Questionnaire on Values (QSV)

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Genitourinary Bacillus Calmette-Guerin Infection after BCG Intravesical Administration in a Patient under Hemodialysis—What Can We Do Better?

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Advances in Infectious Diseases（传染病进展） 2022年第3期

作者： lúcia ribeiro dias catarina almeida fernando nogueira rita soares sofia garcês soares maria lima costa maria teresa boncoraglio filipa ceia

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