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Ingredients from integral valorization of Isabel grape to formulate goat yogurt with stimulatory effects on probiotics and beneficial impacts on human colonic microbiota in vitro

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Food science and Human Wellness 2023年第4期12卷 1331-1342页

作者： Francyeli Araújo silva rita de cássia ram.s do egypto queiroga Evandro Leite de souza Glenise Bierhalz Voss Maria Manuela Estevez Pintado Margarida Angélica da silva Vasconcelos department of Nutrition Federal University of PernambucoRecife 50670-420Brazil department of Nutrition Health Sciences CenterFederal University of ParaíbaJoão Pessoa 58050-585Brazil Universidade católica Portuguesa CBQF-Centro de Biotecnologia e Química Fina-Laboratório AssociadoEscola Superior de BiotecnologiaPorto 4169-005Portugal

Isabel grape(IG)products have high contents of phenolic compounds and fiber recognized for their positive impacts on microorganisms associated with health benefits to host.This study evaluated the effects of goat yogurts formulated with ingredients from IG integral valorization on the growth and metabolism of different probiotic strains,as well as on the population of selected bacterial groups and metabolic activity of human colonic microbiota in vitro.Goat yogurts with IG ingredients(IGI)stimulated the growth of tested Lactobacillus and Bifidobacterium probiotic strains during a 48-h cultivation,as well as decreased the pH values and enhanced the organic acid production.Goat yogurts with IGI increased the population of Lactobacillus spp.and Bifidobacterium spp.during a 24-h in vitro colonic fermentation.A stable Firmicutes:Bacteroidetes ratio close to 1 was found in media with goat yogurt formulations during the colonic fermentation,being similar to the effect caused by fructooligosaccharides.Goat yogurt formulations with IGI caused increased production of short-chain fatty acids and sugar consumption during colonic fermentation.Goat yogurts with IGI should be a valuable strategy for development of novel added-value foods with beneficial effects on gut microbiota and human health.

关键词： Goat dairy product Grape Beneficial microorganisms Gut microbiota Modulatory effects

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How does anodization time affect morphological and photocatalytic properties of iron oxide nanostructures?

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Journal of Materials science & Technology 2020年第3期38卷 159-169页

作者： Bianca Lucas-Granados rita sánchez-Tovar ramón MFernández-domene JoséMaría Estívalis-Martínez JoséGarcía-Antón Ingeniería Electroquímica y corrosión(IEc).Instituto Universitario de seguridad Industrial Radiofísica y Medioambiental(ISIRYM)Universitat Politècnica de ValènciaCamino de Vera s/n46022ValenciaSpain departmento de Ingeniería Química Universitat de ValènciaAv de les Universitatss/n46100 BurjassotSpain

Iron oxide nanostructures are promising materials to be used as photocatalysts in different photoelectrochemical applications. There are different techniques in order to synthesize these nanostructures, but one of the most inexpensive and simple method is electrochemical anodization. This method can lead to different nanostructures by controlling its parameters. Anodization time is one of the most critical parameters since it considerably affects the properties of the obtained nanostructures. In this work, different anodization times(5, 10, 15, 30 and 60 min) were studied. The resulting nanotubes were characterized by field emission scanning electron microscopy, raman laser confocal microscopy, water splitting measurements, Mott-schottky analysis and electrochemical impedance spectroscopy, in order to test their viability for being used as photocatalysts in photoelectrochemical applications. Results showed that the best photocurrent density values in water splitting tests(0.263 m A m-2) were achieved for the sample anodized for 10 min under hydrodynamic conditions.

关键词： Iron oxide Metallic nanostructures Photoelectrochemistry Anodization Time

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Antifeedant Activity of caesalpinia coriaria Essential Oil Against Incisitermes marginipennis (Latreille)

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Phyton-International Journal of Experimental Botany 2021年第3期90卷 907-920页

作者： cesar B.ramírez-López rita Beltrán-sánchez Alistair Hernández-Izquierdo JoséLuis salvador-Hernández Eduardo salcedo-Pérez Rosa E.del Río Mauro M.Martínez Pacheco Instituto de Investigaciones Químico Biológicas Universidad Michoacana de San Nicolás de HidalgoMorelia58030Mexico departamento de Botánica y Zoología CUCBA Universidad de GuadalajaraNextipac45200Mexico

This study scrutinized the possibility of finding toxicant or deterrent plant metabolites against the dry wood termite Incisitermes marginipennis(Latreille).Plant deterrent agents act as repellents or antifeedants to prevent wood decay and increase its useful life.The potential of the tree caesalpinia coriaria(Fabaceae)as a biological source of molecules with deterrent effects against the dry wood termite was assessed by a phytochemical fractionation guided by repellence and antifeedant activities.The gas chromatography-mass spectrometry analysis of the leaf essential oil showed geraniol to be one of the major components and its repellent and antifeedant effects were determined.Geraniol had only an antifeedant effect without affecting the body weight or survival of the dry wood termite.Unlike the leaf essential oil,geraniol did not exhibit a repellency effect.An in-silico approach of the activity of acetylcholinesterase in interaction with geraniol resulted in an affinity energy of−7.5 Kcal/mol.Geraniol interacted with the amino acid tyrosine 324 located in the enzyme’s active site while citronellol(negative control)interacted with tryptophan 83 located adjacent to the active site.These deterring terpenes have not been implemented for the preservation and restoration of wood products exposed to the attack of the dry wood termite.However,they are an important natural control alternative.

关键词： Termite geraniol repellent antifeedant deterrent

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Opportunistic Infections in Renal Transplantation <br/>—A case series

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Open Journal of Pathology 2017年第1期7卷 13-19页

作者： rita sampaio José Palla Garcia Leonídio Dias La salete Martins José ramón Vizcaíno department of Pathology Hospital de Santo António Centro Hospitalar do Porto Porto Portugal department of Nephrology Hospital de Santo António Centro Hospitalar do Porto Porto Portugal

Background: Porto’s Hospital centre is one of the most active Portuguese hospitals in renal transplantation (performed since 1983). Although increasingly rare, opportunistic infections in transplanted patients are associated with high mortality rate in kidney transplantation and remain a major diagnostic challenge. Methods: We investigated 2041 cases of hospital admissions (from 2004 to 2012), any time after kidney transplantation. We described the infection location, the diagnostic techniques used and the mortality after the infection. Results: We found 82 cases of opportunistic infection caused by Herpes virus (Zoster and simplex), cytomegalovirus, Polyomavirus, Aspergilus, Alternaria, Mucorales, candida, Mycobacterium tuberculosis, cryptococcus and Pneumocystis. conclusions: In this article we highlight the important role of histology/cytology in the diagnostic process of these infections. In many cases prompt diagnosis and treatment are necessary to avoid life-threatening complications and may greatly improve prognosis.

关键词： Transplant Pathology Kidney Opportunistic Infections

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Potential seed germination-enhancing plant growth-promoting rhizobacteria for restoration of Pinus chiapensis ecosystems

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Journal of Forestry Research 2021年第5期32卷 2143-2153页

作者： cristina domínguez-castillo Julia María Alatorre-cruz dolores castañeda-Antonio Jose Antonio Munive Xianwu Guo Jesús Francisco López-Olguín Luis Ernesto Fuentes-ramírez Ricardo carreño-López centro de Investigaciones en ciencias Microbiológicas Instituto de CienciasBenemérita Universidad Autónoma de PueblaPueblaMéxico Universidad Autónoma de Querétaro QuerétaroMéxico centro de Biotecnología Genómica Instituto Politécnico NacionalReynosaTamaulipasMéxico

Rhizosphere soil samples of three Pinus chiapensis sites were analyzed for their physicochemical properties,soil bacteria isolated and screened in vitro for growthpromoting abilities.Nine isolates that showed promise were identified to five genera Dyella,Luteimonas,Euterobacter,Paraburkholderia and Bacillus based on the sequences of16 s rRNA gene.All the strains were isolated from nondisturbed stands.These bacteria significantly decreased germination time and increased sprout sizes.Indole acetic acid and gibberellin production and phosphate solubilisation were detected.Results indicate that these biochemicals could be essential for P.chiapensis distribution and suggest the possibility that PGPR inoculation on P.chiapensis seeds prior to planting could improve germination and possibly seedling development.

关键词： Pinus chiapensis Plant growth-promoting rhizobacteria Dyella Luteimonas Enterobacter

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Differential Effect on Two Immobility Responses by chronic Administration of 1,3-di-o-Tolyl-Guanidine (sigma Receptor Agonist) in Rats with Neonatal Ventral Hippocampal Lesion

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Pharmacology & Pharmacy 2014年第7期5卷 681-690页

作者： Blanca Estela Jaramillo Fidel de la cruz Genaro Vargas Oscar O. Morales Gonzalo Flores sergio R. Zamudio Linda Garcés-ramírez departamento de Fisiología Escuela Nacional de Ciencias Biológicas Instituto Politécnico Nacional México D. F. México Instituto de Fisiología Benemérita Universidad Autónoma de Puebla Puebla México Programa Educativo de la Ingeniería en Biotecnología Universidad Politécnica de Pachuca Zempoala Hidalgo México

The effect of chronic administration of the sigma agonist: 1,3-di-o-tolyl-guanidine (DTG), in neonatal ventral hippocampal lesioned (nVHL) rats, on the immobility by clamping and dorsal immobility, were investigated. The nVHL increases the duration of immobility by clamping, but does not affect the duration of the dorsal immobility. We found that DTG augments the duration of the dorsal immobility in the unlesioned rats, but does not modify the duration of immobility induced by clamping the neck. DTG also counteracts the increase in the duration of the immobility by clamping produced by nVHL. However, the increase in the duration of the dorsal immobility produced by DTG is counteracted by nVHL. These results are discussed with respect to the differential effect on the two immobility responses tested, suggesting that they are different forms of immobility mediated by different mechanisms although they behaviorally share common characteristics.

关键词： Immobility Response sigma Receptor DTG schizophrenia Neonatal Ventral Hippocampal Lesion

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Immobility Responses Affected by Potassium in Old Rats

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Pharmacology & Pharmacy 2020年第9期11卷 235-250页

作者： Linda Garces-ramirez Oscar OMorales-Dionisio Gonzalo Flores JoseLuna-Munoz Fidel de la cruz departamento de Fisiologia“Mauricio Russek Berman” Escuela Nacional de Ciencias BiologicasInstituto Politecnico NacionalCiudad de MexicoMexico Laboratorio de Neuropsiquiatria Instituto de FisiologiaBenemerita Universidad Autonoma de PueblaPueblaMexico National dementia BioBank Ciencias BiologicasFacultad de Estudios SuperioresCuautitlanUNAMEstado de MexicoMexico Banco Nacional de cerebros-UNPHU Universidad Nacional Pedro Hernriquez UrenaSanto DomingoRepublica Dominicana

Four immobility responses (IR): elicited by clamping, bandaging, grasping and inversion, and their modification by potassium and spironolactone was studied in old Wistar rats (body weight, 500 g). When undrugged, only... 详细信息

Four immobility responses (IR): elicited by clamping, bandaging, grasping and inversion, and their modification by potassium and spironolactone wspan>as studied in old Wistar rats (body weight, 500 g). When undrugged, only clamping and grasping, but not bandaging and inversion induced an IR in rats. Potassium and spironolactone significantly enhanced the duration of IR induced by clamping but not by grasping. They also induced an immobility response by bandaging, but not by inversion. The data suggest that IR induced by clamping and bandaging are somehow related to changes in the potassium serum levels. Consequently, such a relationship may be a suitable model to study some forms of paralysis in human beings which are related to changes in the potassium serum levels.

关键词： Immobility Responses (IR) Potassium spironolactone Hyperkalemia Familial Periodic Paralysis

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steroidal Plant Growth Promoters vs. Phytopathogens, via Enzymatic Regulation;An in <i>silico</i>Approach

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Advances in Enzyme Research 2021年第4期9卷 55-71页

作者： Alan carrasco-carballo Emiliano Marín-Merino Penélope Merino-Montiel Blanca colin-Lozano sandra Luz cabrera Hilerio Jazmin ciciolil Hilario-Martínez Jesús sandoval-ramírez Laboratorio de Elucidación y síntesis en Química Orgánica Facultad de Ciencias Químicas Benemérita Universidad Autónoma de Puebla Puebla México Laboratorio de síntesis y Modificación de Productos Naturales Facultad de Ciencias Químicas Benemérita Universidad Autónoma de Puebla Puebla México Laboratorio de Bromatología Facultad de Ciencias Químicas Benemérita Universidad Autónoma de Puebla Puebla México 4Facultad de Estudios Superiores-Zaragoza UNAM CDMX México Facultad de Estudios superiores-Zaragoza UNAM CDMX México

steroidal plant growth promoters (sPGP) have been continuously studied due to their high activity increasing biomass and resistance to diverse stress factors. In our hands, a new sPGP family of 22-oxocholestanic comp... 详细信息

style="margin-left:10.0pt;"> Steroidal plant growth promoters (SPGP) have been continuously studied due to their high activity increasing biomass and resistance to diverse stress factors. In our hands, a new SPGP family of 22-oxocholestanic compounds stands out at a comparative level to brassinosteroids (BSs). The potential activity of new SPGP against phytopathogens was studied through in silico molecular docking, these assays were performed with relevant ensymes of phytopatogens Chitinase B and 1,3-span>β-Glucanase. Nine Chitinase B inhibitors and two 1,3-β-Glucanase inhibitors were proposed. The launched study analyzed the interactional and spatial level, determining the presence of interactions with key amino acids in receptors in comparison to reference inhibitors. Even more, the AVR4 and ECP6 effectors were also examined. No compound that blocks ECP6 was found;due to, probably, the influence of its highly hydrophilic environment. In the case of AVR4, two SPGP showed a better docking score (DS) than a chitin fragment (endogenous ligand);this fact demonstrates the latent potential of the 22-oxocholestanic derivatives against phytopathogens, with a specific regulation via proliferation inhibition. Moreover, this SPGP does not affect the symbiotic fungi that are beneficial for the natural plant system.

关键词： 22-Oxocholestanes Brassinosteroids chitinase B 1 3-β-Glucanase ARV4 EcP6

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Presence of Mycoplasma spp.in Patients with Asthma or Allergic Rhinitis

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Advances in Microbiology 2014年第11期4卷 720-725页

作者： Munoz-Zurita Guillermo Paz Martínez David Yánez santos Jorge Antonio Gil Juárez constantino cedillo ramírez María Lilia Alumno de doctorado del Posgrado en ciencias Ambientales Benemérita Universidad Autónoma de PueblaPueblaMexico Hospital Universitario de Puebla Benemérita Universidad Autónoma de PueblaPueblaMexico Facultad de Estomatología Benemérita Universidad Autónoma de PueblaPueblaMexico centro de Investigaciones en ciencias Microbiológicas Benemérita Universidad Autónoma de PueblaPueblaMexico centro de detección Biomolecular VIEPBenemérita Universidad Autónoma de PueblaPueblaMexico

Objective: To compare the presence of Mycoplasma spp. in pharyngeal swabs of patients with asthma, allergic rhinitis and controls, also to compare the reactivity to allergens in the three groups. Material and Methods: Throat swabs of 120 patients with asthma, 211 allergic rhinitis patients and 171 healthy persons were cultured in E media. Positive cultures were identified by PcR. susceptibility of patients positive for isolation of Mycoplasma spp. to indoor, forest and undergrowth allergens were determined. Results: Mycoplasma spp. was isolated in 26.6% of samples from asthmatic patients, 36% in patients with allergic rhinitis and 2.9% in the group of healthy persons. Positive patients for Mycoplasma with asthma and allergic rhinitis showed susceptibility tomite, grass and thunder mainly. conclusion: There is a higher incidence of Mycoplasma spp. in asthmatic and allergic rhinitis patients than healthy persons. Only patients with asthma or allergic rhinitis showed reactivity to environmental allergens.

关键词： Asthma Allergic Rhinitis Allergens Mycoplasma spp.

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Any Hamiltonian system Is Locally Equivalent to a Free Particle

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World Journal of Mechanics 2012年第5期2卷 246-252页

作者： Elizabeth Galindo-Linares Esperanza Navarro-Morale Gilberto silva-Ortigoza Román suárez-Xique Magdalena Marciano-Melchor ramón silva-Ortigoza Edwin Román-Hernández cIdeTEc-IPN. departamento de Posgrado. área de Mecatrónica. Unidad Profesional Adolfo López Mateos. c.P. México D.F. México Facultad de ciencias Físico Matemáticas de la Benemérita Universidad Autónoma de Puebla Apartado Postal Puebla Pue. México Universidad del Istmo Departamento de Matemáticas Aplicadas Cd. Universitaria Tehuantepec Oax. México

In this work we use the Hamilton-Jacobi theory to show that locally all the Hamiltonian systems with n degrees of freedom are equivalent. That is, there is a canonical transformation connecting two arbitrary Hamiltonian systems with the same number of degrees of freedom. This result in particular implies that locally all the Hamiltonian systems are equivalent to that of a free particle. We illustrate our result with two particular examples;first we show that the one-dimensional free particle is locally equivalent to the one-dimensional harmonic oscillator and second that the two-dimensional free particle is locally equivalent to the two-dimensional Kepler problem.

关键词： Hamiltonian system canonical Transformation

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