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Identification and characterization of resistance and pathogenicity of Enterococcus *** samples of donor breast milk

World Journal of Clinical Pediatrics 2020年第3期9卷 53-62页

作者： Luana Andrade mendes Santana Nivea Nara Novais Andrade Lucas Santana Coelho da Silva Caline Novais Teixeira Oliveira Breno Bittencourt de Brito Fabricio Freire de Melo Claudio Lima Souza Lucas Miranda marques Marcio Vasconcelos Oliveira Oliveira Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil

BACKGROUND Breast milk is the primary source of nutrition for *** babies frequently need nutritional support from Human Milk *** bacterial species of the genus Enterococcus are part of the microbiota of healthy donors,they may contaminate samples of pumped breast *** To identify and characterize the bacterial virulence and resistance in samples isolated from the nipple-areolar region,hands,and breast milk aliquots from donors at the Human Milk Bank of Municipal Hospital EsauMatos in the city of Vitória da Conquista,Bahia State,*** The personal hygiene and sanitation of donors were analyzed with the aim of identifying possible reasons for contamination of pumped *** samples as well as aliquots of unpasteurized and pasteurized milk from 30 participants were *** Enterococcus *** underwent a disk diffusion susceptibility test and molecular biology techniques to determine resistance and virulence *** Enterococcus *** identified in 30%of donors(n=9),and 11 specimens were *** to tetracycline was highly prevalent,being detectable in 63%of the isolates(n=7)and followed by intermediate sensitivity to ciprofloxacin,observed in 27%of the specimens(n=3).The efaA gene was found in 63%(n=7)of the isolates,while the ace gene was detected in 27%(n=3).CONCLUSION This study illustrates the importance of microbiological monitoring by Human Milk Banks and the need for alternatives to prevent the presence of Enterococcus *** hospital settings.

关键词： Enterococcus spp. Breast milk Virulence Human milk Pasteurization Antimicrobial resistanc

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Loop-Mediated Isothermal Amplification (LAMP) for the Detection of <i>Listeria monocytogenes</i>and Major Pathogenic Serotypes

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American Journal of Analytical Chemistry 2014年第16期5卷 1057-1064页

作者： Ana Paula Rocha da Costa Mariana de Lira Nunes Carina Lucena mendes-marques Alzira Maria Paiva de Almeida Nilma Cintra Leal Departamento de Microbiologia Centro de Pesquisas Aggeu Magalh&atilde es FIOCRUZ-PE Recife Brazil

Rapid identification and characterization of Listeria monocytogenes are required for the food industry, epidemiological studies, and disease prevention and control. However, typing procedures are labor-intensive and time-consuming, and they require technical expertise, a panel of sera and reference culture strains or sophisticated and expensive equipment. To improve upon traditional diagnostic methods for L. monocytogenes we developed and evaluated an efficient procedure for the specific identification of L. monocytogenes and the major pathogenic serotypes of the species based on loop-mediated isothermal amplification (LAMP). Four individual reactions were designed using primers targeting any L. monocytogenes serotypes (LAMP-AS) and the 1/2a (LAMP-1/2a), 1/2b (LAMP-1/2b), and 4b (LAMP-4b) serotypes. The procedure distinguished L. monocytogenes from closely genetically related species and the targeted serotypes. Cross-reactivity with a few rare serotypes isolated from food or clinical samples did not impair the usefulness of the procedure. Thus, our approach constitutes a fast, easy and low-cost alternative for L. monocytogenes diagnosis and serotyping and may be useful for surveillance and epidemiological investigation programs.

关键词： Listeria monocytogenes Diagnosis Serotypes Identification LAMP

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Vestibular Function Evaluation in Endemic Diseases Combat Agents

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Health 2021年第2期13卷 144-156页

作者： Patrícia Arruda de Souza Alcarás Bianca Simone Zeigelboim Maria Cristina Alves Corazza Débora Lüders Jair mendes marques.adriana Bender Moreira de Lacerda University of Western Sã o Paulo UNOESTE Presidente Prudente S&#227 o Paulo Brazil Tuiuti University of Paraná UTP Curitiba Paraná Brazil Université de Montréal UdeM Quebec Canada

Introduction: Simultaneous exposure to certain chemical agents and noise may cause synergistic, additive or potentiating effects on the auditory and vestibular system. However, there is still a lack of studies investigating simultaneous exposure to pesticides and noise in the vestibular system. Purpose: To analyze the findings of the vestibular evaluation of endemic diseases combat agents when simultaneously exposed to pesticides and noise. Method: Thirty-three male pesticide-exposed and noise-fighting endemic diseases combat agents participated in the study. The age range was 48 to 67 years (mean = 56 years). All participants underwent vestibular examinations, such as positional nystagmus research, spontaneous nystagmus with open and closed eyes, semi-spontaneous nystagmus, optokinetic nystagmus, pendular screening, per rotatory, caloric tests and tonal audiometry. Data were analyzed by descriptive statistics. Results: Altered exams were presented in 36.4% of the cases. There was no statistically significant relationship between pesticide handling time and test results (p = 0.2825). The results of pure tone audiometry correlated significantly (p = 0.0494) with the vestibular exams, as participants who presented with worse hearing thresholds at the frequency of 4000 Hz in the right ear also presented altered results in the vestibular exam. Conclusion: The present study concluded that more than 1/3 of the studied population presented with vestibular dysfunction, which was related to anterior and posterior labyrinth alteration (Cochlear-Vestibular Syndrome). No central vestibular alteration was noted in this population.

关键词： Community Health Workers Pesticides Noise Hearing Vestibular System

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Evaluation of the neuropharmacological properties of nerol in mice

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World Journal of Neuroscience 2013年第1期3卷 32-38页

作者： Thiago Henrique Costa marques Maria Leonildes Boavista Gomes Castelo Branco marques Douglas dos Santos Lima Halmisson D’arley Santos Siqueira Jose Damasceno Nogueira Neto Maria do Socorro Boavista Gomes Castelo Branco Alexandre Araujo de Souza Damiao Pergentino de Sousa Rivelilson mendes de Freitas Northeast Biotechnology Network Federal University of Piauí(UFPI)TeresinaBrazil Pharmacology Universidade Federal do Piauí(UFPI)TeresinaBrazil Pharmaceutics Sciences Chemistry DepartmentFederal University of Piauí(UFPI)TeresinaBrazil Department of Pharmacy Federal University of Sergipe(UFS)Sao CristovaoBrazil Department of Biochemistry and Pharmacology Campus Ministro Petronio PortelaFederal University of Piauí(UFPI)TeresinaBrazil

The search for therapeutic agents that will provide the ground for man and an improvement in their quality of life is ceaseless. The nerol (cis-2,6-dimethyl-2,6-octadien-8-ol) is a monoterpene which can be found in various medicinal plants as Lippia spp and Melissa officinalis L. The objective of this study was to analyze the acute effect of nerol in the central nervous system (CNS) by performing behavioral tests in mice (open field, elevated plus-maze, light/dark and rota rod tests). We used male albino mice (Mus musculus), Swiss variety, adult with 2 month-old. The animals were divided into five groups (n = 8) for each experimental protocol, and they were administered intraperitoneally (i.p.), respectively, Tween 80 0.05% dissolved in saline solution 0.9%, nerol (30, 60 or 90 mg/kg) or diazepam (2 mg/kg). In the open field test, all groups treated with nerol showed a significant decrease in motor activity (number of crossings, rearings and groomings) when compared with vehicle group. In the elevated plus-maze test, nerol groups significantly increased the number of entries and time of permanence in the open arms when compared with vehicle group. In the light-dark test, nerol groups showed a significant increase the time of permanence in the room clear when compared with vehicle group. In the rota rod test, the groups treated with nerol didn’t show modification in time spent and number of falls in the revolving bar when compared with vehicle group. These results indicate a possible anxiolytic effect of nerol in mice.

关键词： Anxiety Behaviors Mice Nerol Essential Oils

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嘉卿会所-台丰高尔夫俱乐部

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现代装饰 2022年第4期 14-21页

作者： álvaro Siza Carlos Castanheira Elisabete Queirós Catarina Araújo Erika Musci Filipe Mota Joana Soeiro Jo?o Alves Jorge Pinheiro Mariana mendes Nuno Campos Nuno Rodrigues Pedro Afonso rita Saturnino Sara Pinto Sofia Concei??o Carlos Castanheira 范嘉苑

在高尔夫球运动中,力量与优雅的完美平衡是衡量其水平的一个基本准则。同样的,在建筑设计与建造的表达过程中,除了回应技术问题与基本需求以外,还需要考虑其他或简单或复杂的因素。衡量一个项目不仅只是看基本要素,即使它仅需要回应基...

在高尔夫球运动中,力量与优雅的完美平衡是衡量其水平的一个基本准则。同样的,在建筑设计与建造的表达过程中,除了回应技术问题与基本需求以外,还需要考虑其他或简单或复杂的因素。衡量一个项目不仅只是看基本要素,即使它仅需要回应基础功能。好的设计应该经过谨慎的思考,由此承载巨大能量,传达优雅气息。“经验分析”能够帮助我们辨别设计的第一步,它可能源自于一种态度、概念,或者一些难以解释的想法。

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Talking about Giving Up a Child for adoption with Nursing Professionals

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Journal of Health Science 2018年第4期6卷 249-253页

作者： rita Tropa marques Yara Ishara Ivonise Fernandes da Motta Santa Casa da Misericordia de Lisboa Research Laboratory on Creativity and Psychic Development (LAPECRI) AvenidaProfessor Mello de Morais 1721-Butanta Sao Paulo 05508-030 Brazil Tribunal de Justica do Estado de Sao Paulo Research Laboratory on Creativity and Psychic Development (LAPECRI) AvenidaProfessor Mello de Morais 1721-Butanta Sao Paulo 05508-030 Brazil Research Laboratory on Creativity and Psychic Development (LAPECRI) Department of Clinical Psychology University of S6oPaulo Avenida Professor Mello de Morais 1721-Butanta Sao Paulo 05508-030 Brazil

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Placing a Child for adoption and the Myth of Maternal Love

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Journal of Health Science 2019年第5期7卷 295-302页

作者： rita Tropa marques Yara Ishara Ivonise Fernandes da Motta Santa Casa da Misericordia de Lisboa Research Laboratory on Creativity and Psychic Development (LAPECRI)Avenida Professor Mello de Morais1721-Butanta Sao Paulo 05508-030Brazil Tribunal de Justica do Estado de Sao Paulo Research Laboratory on Creativity and Psychic Development (LAPECRI)Avenida Professor Mello de Morais1721-ButantaSao Paulo 05508-030Brazil Research Laboratory on Creativity and Psychic Development (LAPECRI) Department of Clinical PsychologyUniversity of Sao PauloAvenida Professor Mello de Morais1721-Butanta Sao Paulo 05508-030Brazil

The article discusses the circumstances of placing a child for adoption and its relation to the myth of maternal love. It examines the research related to this issue, questioning the idealization of motherhood expressed in the idea that every woman is “naturally” a mother. It also examines the justification commonly found in literature that perceives the act of placing a child for adoption as the result of difficulties associated with socioeconomic factors. It shows the relevance of the research on the subject involving different professionals who deal with this reality.

关键词： Myth of maternal love adoption motherhood

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Dengue in renal transplant recipients: Clinical course and impact on renal function

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World Journal of Transplantation 2017年第1期7卷 57-63页

作者： Paula Frassinetti Castelo Branco Camur?a Fernandes Reed AndréSiqueira Evelyne Santana Gir?o Rainne AndréSiqueira Márcia Uchoa Mota Leyla Castelo Branco Fernandes marques Silvana Cristina Albuquerque Andrade Wilson mendes Barroso S?nia Leite Silva Bruno Gomes Rodrigues dos Santos Claúdia Maria Costa de Oliveira Kidney Transplant Unit Hospital Universitário Walter Cantídio Universidade Federal do Ceará Department of Nephrology of Universidade Estadual do Ceará Universidade Estadual do Ceará

AIM To present clinical characteristics from renal transplant recipients with dengue fever and its impact on graft *** We retrospectively evaluated 11 renal transplant recipients(RTR) with dengue infection confirmed by laboratory test, between January 2007 and July 2012, transplanted in the Renal Transplant Center of Walter Cantídio University Hospital from Federal University of Ceará. RESULTS Positive dengue serology(IgM) was found in all patients. The mean time between transplant and dengue infection was 43 mo. Fever was presented in all patients. Nine patients presented with classical dengue and two(18%) with dengue hemorrhagic fever. All cases had satisfactory evolution with complete recovery of the symptoms. The time for symptom resolution varied from 2 to 20 d, with an average of 9 d. An increase of creatinine after the infection was observed in three(27.2%) patients with no clinically impact on the kidney graft function. CONCLUSION RTR with dengue infection seems to have a clinical presentation and evolution similar to those seen in the general population, with no long-term damage to patient and to the graft.

关键词： Kidney Renal Transplant Dengue Clinical Brazil

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Cardiac Complications in Maroteaux-Lamy Syndrome： Case Report

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Journal of Pharmacy and Pharmacology 2018年第4期6卷 410-414页

作者： Ricardo Siqueira Gongalves Claudia Aparecida marques Landim Jesuita mendes Cornelio Joaquim Tomaz da Silva Graduating in Medicine. Universidade do Estado de Mato Grosso--UNEMAT Liga Academica de Cardiologia e Cirurgia Department of Cardiology and Intensive Medicine--Universidade do Estado de Mato Grosso--UNEMAT Liga Academica de Cardiologia e Cirurgia Cardiovascular (LACCIC) Caceres--MT 78200-000 Brazil Department of Pediatric Nursing--Hospital Municipal de Unai-MG (HMU) Unai-MG 38610-000 Brazil Department of General Surgery~Hospital Municipal de Unai-MG (HMU) Unai--MG 38610-000 Brazil

MLS (Maroteaux-Lamy syndrome) or MPS VI (mucopolysaccharidosis VI) is an autosomal recessive pathology in which there is absence or low activity of the enzyme N-Acetylgalactosamine-4-Sulfatase, which hydrolyzes GAGs (glycosaminoglycans) in the body (mainly dermatan sulfate). Consequently, there occurs lysosomal deposition of GAGs in connective tissue multisystemic. Myocardium and heart valves are frequently affected structures, presenting a direct correlation with the reports of complications and deaths. Case report: RGS, male, 3 years and 2 months, diagnosed with MPS VI from the first month of life, in weekly ERT (enzyme replacement therapy) since 4 months of age (inconstant). At physical examination: normotensive, with holosystolic heart murmur 3+/6+ in mitral focus. Complementary tests: normal electrocardiogram, echocardiogram with pronounced mitral regurgitation, concentric left ventricular hypertrophy of moderate degree and mild aortic insufficiency. Discussion: Mitral valve disease is common in patients with MLS. Conditions such as cardiomyopathy, fibroelastosis, aneurysm and pulmonary hypertension may occur in these patients, indicative of morbidity and mortality. Early and constant ERT may be useful in slowing a progression of heart disease. Conclusions: follow-up with a cardiologist is important to evaluate the progression of cardiac complications in MPS VI. Constant and early ERT provides better prognosis for these patients.

关键词：并发症心脏病症候群案例 MPS ERT 结缔组织 MLS

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Analysis of the Deformation of Composite Materials of Scrapes of Tire and Latex with Temperature Variation

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Journal of Chemistry and Chemical Engineering 2012年第1期6卷 39-47页

作者： Elmo Thiago Lins Coeuras Ford Rubens Maribondo do Nascimento Jose Ubiragi de Lima mendes Antonio Torres marques Celeste Margarida Correia Pereira Technological Center Campus Universitfrio Federal University of Rio Grande do Norte Lagoa Nova-Natal-RN 59072-790Brazil Faculty of Engineering University of Porto Porto 4200-465 Portugal Institute of Mechanical Engineering and Industrial administration University of Porto Porto 4200-465 Portugal

The great major applications of thermal isolation are in the strip of drops and averages temperatures (up to 180 oC), which is used from aggressive materials to the nature, such as glass wool, rock wool, polystyrene, EPS among others. In spite of the effectiveness in the retention of the flow of heat, such materials possess considerable cost and they are discarded for long years to be decomposed. In that context, to adapt to the world politics about the preservation of the environment, a study began with intention of developing a material composite with thermal properties, originating from insulating industrial residues. In this research, the behavior of this composite was analyzed, when it submitted traction efforts. For this, a term-mechanic was used (DMA), where the composite was differentiated under variation temperatures. It was also certain that the gradient temperature of the composite was the source of heat, the conductivity and thermal diffusion. Consequently, the function of the answers of the system was possible to be observed with the deformation of the composite with the tension and temperature variation, as well as the temperature gradient for each proportion of the composite.

关键词：梯度复合材料温度变化变形乳胶轮胎擦伤平均温度聚苯乙烯

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