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In vivo polydopamine coating of Rhodobacter sphaeroides for enhanced electron transfer

Nano Research 2024年第2期17卷 875-881页

作者： Rossella Labarile Danilo Vona Maria Varsalona Matteo Grattieri Melania Reggente Roberto Comparelli Gianluca M.Farinola Fabian Fischer Ardemis A.Boghossian Massimo trotta Istituto per i Processi Chimico Fisici Consiglio Nazionale delle RicercheBari 70126Italy Dipartimento di Chimica Universitàdegli Studi di BariBari 70126Italy Institute of Chemical Sciences and Engineering(ISIC) Ecole Polytechnique Fédérale de Lausanne(EPFL)Lausanne 1015Switzerland Institute of Life Technologies HES-SO Valais-Wallis1950 SionSwitzerland Institute for Renewable Energy HES-SO Valais-Wallis1950 SionSwitzerland

Recent advances in coupling light-harvesting microorganisms with electronic components have led to a new generation of biohybrid devices based on microbial *** devices are limited by the poorly conductive interface between phototrophs and synthetic materials that inhibit charge *** study focuses on overcoming this bottleneck through the metabolically-driven encapsulation of photosynthetic cells with a bio-inspired conductive *** of the purple non sulfur bacterium Rhodobacter sphaeroides were coated with a polydopamine(PDA)nanoparticle layer via the self-polymerization of dopamine under anaerobic *** treated cells show preserved light absorption of the photosynthetic pigments in the presence of dopamine concentrations ranging between 0.05–3.5 *** thickness and nanoparticle formation of the membrane-associated PDA matrix were further shown to vary with the dopamine concentrations in this *** to uncoated cells,the encapsulated cells show up to a 20-fold enhancement in transient photocurrent measurements under mediatorless *** biologically synthesized PDA can thus act as a matrix for electronically coupling the light-harvesting metabolisms of cells with conductive surfaces.

关键词： bioelectronics photosynthetic bacteria purple bacteria electron transfer polydopamine biophotovoltaics

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Polydopamine-coated photoautotrophic bacteria for improving extracellular electron transfer in living photovoltaics

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Nano Research 2024年第2期17卷 866-874页

作者： Melania Reggente Charlotte Roullier Mohammed Mouhib Patricia Brandl Hanxuan Wang Stefano Tacconi Francesco Mura Luciana Dini Rossella Labarile Massimo trotta Fabian Fischer Ardemis A.Boghossian Institute of Chemical Sciences and Engineering(ISIC) Ecole Polytechnique Fédérale de Lausanne(EPFL)Lausanne 1015Switzerland Department of Biology and Biotechnology Charles Darwin Sapienza University of RomeRome 00185Italy Research Center on Nanotechnology Applied for Engineering of Sapienza(CNIS) University of Rome SapienzaRome 00185Italy CNR-IPCF Consiglio Nazionale delle Ricerche–Istituto per i Processi Chimico Fisici Bari 70126Italy Institute of Life Technologies HES-SO Valais-WallisSion 1950Switzerland Institute for Renewable Energy HES-SO Valais-WallisSion 1950Switzerland

Living photovoltaics are microbial electrochemical devices that use whole cell–electrode interactions to convert solar energy to *** bottleneck in these technologies is the limited electron transfer between the microbe and the electrode *** study focuses on enhancing this transfer by engineering a polydopamine(PDA)coating on the outer membrane of the photosynthetic microbe Synechocystis *** coating provides a conductive nanoparticle shell to increase electrode adhesion and improve microbial charge extraction.A combination of scanning electron microscopy(SEM),transmission electron microscopy(TEM),UV–Vis absorption,and Raman spectroscopy measurements were used to characterize the nanoparticle shell under various synthesis *** cell viability and activity were further assessed through oxygen evolution,growth curve,and confocal fluorescence microscopy *** results show sustained cell growth and detectable PDA surface coverage under slightly alkaline conditions(pH 7.5)and at low initial dopamine(DA)concentrations(1 mM).The exoelectrogenicity of the cells prepared under these conditions was also characterized through cyclic voltammetry(CV)and chronoamperometry(CA).The measurements show a three-fold enhancement in the photocurrent at an applied bias of 0.3 V(***/AgCl[3 M KCl])compared to non-coated *** study thus lays the framework for engineering the next generation of living photovoltaics with improved performances using biosynthetic electrodes.

关键词： biophotovoltaics bioelectronics photosynthetic bacteria cyanobacteria polydopamine adherence

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Melanocortin 3,5 receptors immunohistochemical expression in colonic mucosa of inflammatory bowel disease patients:A matter of disease activity?

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World Journal of Gastroenterology 2024年第9期30卷 1132-1142页

作者： Antonietta Gerarda Gravina Iacopo Panarese Maria Consiglia trotta Michele D'Amico Raffaele Pellegrino Franca Ferraraccio Marilena Galdiero Roberto Alfano Paolo Grieco Alessandro Federico Hepatogastroenterology Division Department of Precision MedicineUniversity of Campania Luigi VanvitelliNaples 80138Italy Pathology Division Department of Mental and Physical Health and Preventive MedicineUniversity of Campania Luigi VanvitelliNaples 80138Italy Department of Experimental Medicine Division of PharmacologyUniversity of Campania Luigi VanvitelliNaples 80138Italy Department of Advanced Medical and Surgical Sciences(DAMSS) University of Campania Luigi VanvitelliNaples 80138Italy Department of Pharmacy University of Naples Federico IINaples 80131Italy

BACKGROUND Melanocortin 3 and 5 receptors(i.e.,MC3R and MC5R)belong to the melanocortin ***,data regarding their role in inflammatory bowel diseases(IBD)are currently *** This study aims to ascertain their expression profiles in the colonic mucosa of Crohn’s disease(CD)and ulcerative colitis(UC),aligning them with IBD disease endoscopic and histologic *** Colonic mucosal biopsies from CD/UC patients were sampled,and immunohisto-chemical analyses were conducted to evaluate the expression of MC3R and *** sampling was performed on both traits with endoscopic scores(Mayo endoscopic score and CD endoscopic index of severity)consistent with inflamed mucosa and not consistent with disease activity(i.e.,normal appearing mucosa).RESULTS In both CD and UC inflamed mucosa,MC3R(CD:+7.7 fold vs normal mucosa,P<0.01;UC:+12 fold vs normal mucosa,P<0.01)and MC5R(CD:+5.5 fold vs normal mucosa,P<0.01;UC:+8.1 fold vs normal mucosa,P<0.01)were significantly more expressed compared to normal *** MC3R and MC5R are expressed in the colon of IBD ***,expression may differ according to disease endoscopic activity,with a higher degree of expression in the traits affected by disease activity in both CD and UC,suggesting a potential use of these receptors in IBD pharmacology.

关键词： Melanocortin 3 receptor Melanocortin 5 receptor Ulcerative colitis Crohn's disease Inflammatory bowel disease

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Predictive immune biomarkers: an unattainable chimera?

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Cellular & Molecular Immunology 2018年第8期15卷 740-742页

作者： Anna Maria trotta Roberto Pacelli Stefania Scala Functional Genomics Istituto Nazionale per lo Studio e la Cura dei TumoriFondazione‘G.Pascale’Via SemmolaNaplesItaly Dipartimento di Scienze Biomediche Avanzate.UniversitàFederico II.Via Pansini NaplesItaly

Immune checkpoint blockade(ICB)with antibodies interfering with cytotoxic T lymphocyte antigen 4(CTLA-4)and the programmed cell death 1(PD-1)protein tremendously revolutionized therapy for advanced ***,the activity of such agents(ipilimumab and nivolumab)is limited to a 10–45%response rate in the context of unselected populations with advanced solid *** common cancer types have demonstrated a very low frequency of response(breast,prostate and colon cancers),and heterogeneous responses have been observed between distinct tumors within the same *** to broad clinical applicability include identifying patients most likely to benefit from checkpoint inhibitors and overcoming resistance to *** immune response is a dynamic and constantly evolving process due primarily to patient-dependent factors,including genetic and tumor microenvironment(TME)features,and secondarily to treatment interventions.

关键词： immune colon likely

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Contrasting responses of native and alien plant species to soil properties shed new light on the invasion of dune systems

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Journal of Plant Ecology 2020年第6期13卷 667-675页

作者： Stefano Vitti Elisa Pellegrini Valentino Casolo Giacomo trotta Francesco Boscutti Department of Life Sciences University of TriesteVia L.Giorgieri 1034127 TriesteItaly Department of Agricultural FoodEnvironmental and Animal SciencesUniversity of Udinevia delle Scienze 91Udine 33100Italy Department of Biology University of CopenhagenUniversitetsparken 42100 KøbenhavnØDenmark

Aims Among terrestrial ecosystems,coastal sandy dunes are particularly prone to alien plant *** studies related the invasion of dune habitats to anthropic causes,but less is known about the role of soil properties and plant traits in plant *** this study,we tested the relationships between soil features and alien plant invasion in dune systems,focusing on the interplay between soil nutrients,soil salinity and plant functional *** Study sites were sandy barrier islands of the Marano and Grado lagoon(northern Adriatic Sea).One hundred plots(4 m×4 m)were selected within 10 areas according to the main habitats occurring along the ecological gradient of dune system(foredune,backdune and saltmarsh).In each plot,we recorded all plant species occurrence and abundance and we collected a soil *** each soil sample,soil texture,conductivity(as proxy of soil salinity),organic carbon and nitrogen content were analyzed and related to the species number and cover of native and alien *** of main reproductive and vegetative functional traits among habitats was also analyzed for both alien and native *** Findings Soil properties were strongly related to overall plant diversity,by differently affecting alien and native species *** backdune,the most invaded habitat,a high soil conductivity limited the number of alien species,whereas the content of soil organic carbon increased along with alien plant abundance,suggesting also the occurrence of potential feedback processes between plant invasion and *** found a significant convergence between native and alien plant functional trait spectra only in backdune habitat,where environmental conditions ameliorate and plant competition *** findings suggest that in harsh conditions only native specialized plants can thrive while at intermediate conditions,soil properties gradient acts in synergy with plant traits to curb/facilitate alien plant richness.

关键词： invasive alien species functional traits soil nutrients plant communities dune system

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Effects of dielectric stoichiometry on the photoluminescence properties of encapsulated WSe2 monolayers

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Nano Research 2018年第3期11卷 1399-1414页

作者： Javier Martfn-Sanchez Antonio Mariscal Marta De Luca Aitana Tarazaga Martin-Luengo Georg Gramse Alma Halilovic Rosalia Serna Alberta Bonanni Ilaria Zardo rinald. trotta Armando Rastelli Institute of Semiconductor and Solid State Physics Johannes Kepler University Linz Altenbergerstrasse 69 A-4040 Linz Austria Laser Processing Group Instituto de Optica CSIC C/Serrano 121 28006 Madrid Spain Department of Physics University of Basel Klingelbergstrasse 82 4056 Basel Switzerland Institute for Biophysics Johannes Kepler University Linz Gruberstrasse 40 A-4020 Linz Austria

Two-dimensional transition metal dichalcogenide semiconductors have emerged as promising candidates for optoelectronic devices with unprecedented properties and ultra-compact footprints. However, the high sensitivity of atomically thin materials to the surrounding dielectric media imposes severe limitations on their practical applicability. Hence, to enable the effective integration of these materials in devices, the development of reliable encapsulation procedures that preserve their physical properties is required. Here, the excitonic photoluminescence （at room temperature and 10 K） is assessed on mechanically exfoliated WSe2 monolayer flakes encapsulated with SiOx and AlxOy layers by means of chemical and physical deposition techniques. Conformal coating on untreated and non- functionalized flakes is successfully achieved by all the techniques examined, with the exception of atomic layer deposition, for which a cluster-like oxide coating is formed. No significant compositional or strain state changes in the flakes are detected upon encapsulation, independently of the technique adopted. Remarkably, our results show that the optical emission of the flakes is strongly influenced by the stoichiometry quality of the encapsulating oxide. When the encapsulation is carried out with slightly sub-stoichiometric oxides, two remarkable phenomena are observed. First, dominant trion （charged exciton） photoluminescence is detected at room temperature, revealing a clear electrical doping of the monolayers. Second, a strong decrease in the optical emission of the monolayers is observed, and attributed to non-radiative recombination processes and/or carrier transfer from the flake to the oxide. Power- and temperature-dependent photoluminescence measurements further confirm that stoichiometric oxides obtained by physical deposition lead to a successful encapsulation, opening a promising route for the development of integrated two-dimensional devices.

关键词： two dimensional (2D)materials dielectric encapsulation transition metaldichalcogenides semiconductors photoluminescence WSe2

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Understanding Reputational Crisis： Evidence From the European Banking Sector

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Journal of Modern Accounting and Auditing 2012年第2期8卷 247-266页

作者： Stefano Dell＇Atti Antonella Iannuzzi Giusy Cavallaro Annarita trotta University of Foggia Italy University ＂Magna Graecia＂ of Catanzaro Italy

This paper presents a comparative qualitative analysis of reputational crisis of four European banks, and explores how in recent years these companies have faced the manifestation of reputational risk. To achieve this, the research follows three related steps：（1） to carry out a review of the literature on reputational risk in the banking sector aimed to identify the relationships between causes, effects, stakeholders, and key qualitative-quantitative variables involved during the reputational crisis of a bank; （2） to propose a conceptual framework for management of reputational risk （and reputational crisis） in banking; （3） to test this framework with the results of an empirical analysis, carried out through the observation of key variables of some reputational crisis of intemational banks. The main results show that：（1） the banks are not yet prepared to accurately manage a reputational crisis or to prevent them; （2） the reputational crisis is determined by several internal and external factors; （3） the conduct of the managers and the corporate communication are very important to overcome a reputational crisis. Finally, this research provides indications that will help banks to better manage their corporate reputation and prevent reputational crisis.

关键词： reputational risk reputational crisis reputational crisis management bankruptcy financial/bankingsector

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CXCR4 expression affects overall survival of HCC patients whereas CXCR7 expression does not

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Cellular & Molecular Immunology 2015年第4期12卷 474-482页

作者： Maria Neve Polimeno Caterina Ierano Crescenzo D'Alterio Nunzia Simona Losito Maria Napolitano Luigi Portella Giosuè Scognamiglio Fabiana Tatangelo Anna Maria trotta Steven Curley Susan Costantini Raffaele Liuzzi Francesco Izzo Stefania Scala Oncological Immunology Istituto Nazionale per lo Studio e la Cura dei Tumori "Fondazione Giovanni Pascale"-IRCCS-ITALIA Naples Italy. Pathology Unit Istituto Nazionale per lo Studio e la Cura dei Tumori "Fondazione Giovanni Pascale"-IRCCS-ITALIA Naples Italy. Department of Surgical Oncology University of Texas MD Anderson Cancer Center Houston TX USA. CROM-Centro Ricerche Oncologiche Mercogliano Mercogliano (AV) Italy. Institute of Biostructure and Bioimaging (IBB) of the Italian National Research Council Naples Italy. Hepato-Biliary Surgery Unit Istituto Nazionale per lo Studio e la Cura dei Tumori "Fondazione Giovanni Pascale"-IRCCS-ITALIA Naples Italy.

Hepatocellular carcinoma （HCC） is a heterogeneous disease with a poor prognosis and limited markers for predicting patient survival. Because chemokines and chemokine receptors play numerous and integral roles in HCC disease progression, the CXCR4-CXCL12-CXCR7 axis was studied in HCC patients. CXCR4 and CXCR7 expression was analyzed by immunohistochemistry in 86 HCC patients （training cohort） and validated in 42 unrelated HCC patients （validation cohort）. CXCR4 levels were low in 22.1% of patients, intermediate in 30.2%, and high in 47.7%, whereas CXCR7 levels were low in 9.3% of patients, intermediate in 44.2% and high in 46.5% of the patients in the training cohort. When correlated to patient outcome, only CXCR4 affected overall survival （P=-O.03）. CXCR4-CXCL12-CXCR7 mRNA levels were examined in 33/86 patients. Interestingly, the common CXCR4-CXCR7 ligand CXCL12 was expressed at significantly lower levels in tumor tissues compared to adjacent normal liver （P=0.032）. The expression and function of CXCR4 and CXCR7 was also analyzed in several human HCC cell lines. CXCR4 was expressed in Huh7, Hep3B, SNU398, SNU449 and SNU475 cells, whereas CXCR7 was expressed in HepG2, Huh7, SNU449 and SNU475 cells. Huh7, SNU449 and SNU475 cells migrated toward CXCL12, and this migration was inhibited by AMD3 lO0/anti-CXCR4 and by CCX771/anti-CXCR7. Moreover, SNU449 and Huh7 cells exhibited matrix invasion in the presence of CXCL12 and CXCL11, a ligand exclusive to CXCR7. In conclusion, CXCR4 affects the prognosis of HCC patients but CXCR7 does not. Therefore, the CXCR4-CXCL12-CXCR7 axis plays a role in the interaction of HCC with the surrounding normal tissue and represents a suitable therapeutic target.

关键词： CXCR4 CXCR7 CXCL12 hepatocellular carcinoma prognosis

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Mechanochemical Synthesis and Characterization of Molybdenum Dioxide-Hematite Nanostructures with Different Molarities

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Journal of Minerals and Materials Characterization and Engineering 2018年第6期6卷 587-600页

作者： Matthew Knauss Felicia Tolea Mihaela Valeanu Lucian Diamandescu Richard trotta Kelly Wood Agnieszka Grabias Monica Sorescu Department of Physics Duquesne University Pittsburgh USA Department of Materials Science National Institute of Materials Physics Bucharest-Magurele Romania Department of Materials Science Institute of Electronic Materials Technology Warsaw Poland

xMoO2-(1-x)α-Fe2O3 nanoparticle system with molarities x = 0.1, 0.3, 0.5 and 0.7 was successfully synthesized by mechanochemical activation of MoO2 and α-Fe2O3 mixtures for 0 - 12 hours of ball milling time. X-ray powder diffraction (XRD), Mössbauer spectroscopy and magnetic measurements were used to study the phase evolution of the molybdenum dioxide-hematite nanoparticle system under the mechanochemical activation process. Rietveld refinement of the XRD patterns yielded the values of the crystallite size and lattice parameters as function of milling times and indicated the presence of Mo-substituted hematite and Fe-doped molybdenum dioxide at long milling times. The Mössbauer studies yielded the magnetic hyperfine fields and the relative abundance of a quadrupole-split doublet as function of the milling time for all molar concentrations involved. Recoilless fraction was determined using our dual absorber method and was found to decrease with increasing ball milling time. Magnetic measurements recorded at 5 and 300 K in an applied magnetic field of 50,000 Oe showed the magnetic properties in the antiferromagnetic and canted ferromagnetic states. The Morin transformation was evidenced by zero-field cooling-field cooling (ZFC-FC) measurements in a magnetic field of 200 Oe.

关键词： Milling Spectroscopy Magnetic Properties

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Most People Can＇t Live Without High-speed Internet

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英语画刊（高三·社会综合版） 2011年第4期 8-8页

作者： Daniel trotta

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