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Simple and robust 3D MINFLUX excitation with a variable phase plate

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Light(Science & Applications) 2024年第7期13卷 1354-1363页

作者： Takahiro Deguchi jonas ries European Molecular Biology Laboratory Cell Biology and BiophysicsHeidelbergGermany. Max Perutz Labs Vienna Biocenter Campus(VBC)ViennaAustria. University of Vienna Center for Molecular BiologyDepartment of Structural and Computational BiologyViennaAustria. University of Vienna Faculty of PhysicsViennaAustria

MINFLUX has achieved extraordinary resolution in superresolution imaging and single fluorophore *** is based on localizing single fluorophores by rapid probing with a patterned beam that features a local intensity *** implementations,however,are complex and expensive and are limited in speed and ***,we show that a combination of an electro-optical modulator with a segmented birefringent element such as a spatial light modulator produces a variable phase plate for which the phase can be scanned on the MHz *** or top-hat phase patterns generate high-contrast compact excitation point-spread functions for MINFLUX localization in the x,y,and z-direction,respectively,which can be scanned across a fluorophore within a microsecond,switched within 60 microseconds and alternated among different excitation *** discuss how to compensate for non-optimal performance of the components and present a robust 3D and multi-color MINFLUX excitation module,which we envision as an integral component of a high-performance and cost-effective open-source MINFLUX.

关键词： excitation phase switched

Real meromorphic functions and linear differential polynomials

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Science China Mathematics 2010年第3期53卷 739-748页

作者： langley j. K. School of Mathematical Sciences University of Nottingham

We determine all real meromorphic functions f in the plane such that f has finitely many zeros, the poles of f have bounded multiplicities, and f and F have finitely many non-real zeros, where F is a linear differential polynomial given by F = f (k) +Σk-1j=0ajf(j) , in which k≥2 and the coefficients aj are real numbers with a0≠0.

关键词： non-real zeros meromorphic function linear differential polynomial

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Using Inductance as a Tuning Parameter for RF Meta-atoms

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Nano-Micro Letters 2012年第2期4卷 103-109页

作者： Derrick langley Ronald A.Coutu Peter j.Collins Air Force Institute of Technology Wright-Patterson Air Force Base

The resonant frequency of metamaterials structured with split ring resonator(SRR) meta-atoms is determined primarily through the capacitance and inductance of the individual meta-atoms. Two designs that vary inductance incrementally were modeled, simulated, fabricated, and tested to investigate the role inductance plays in metamaterial designs. The designs consisted of strategically adding sections to the SRR to increase the inductance, but in a manner that minimized capacitance variations. Each design showed a shift in resonant frequency that was proportional to the length of the added section. As the length of each section was increased, the resonant frequency shifted from 2.78 GHz to 2.18 GHz.

关键词： Inductance Meta-atom Metamaterial Split Ring Resonator Radio Frequency Resonance

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Slit Die Optimisation Employing A Gradient Free Method

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Slit Die Optimisation Employing A Gradient Free Method

The Fourth World Congress of Structural and Multidisciplinary Optimization

作者： j.Sienz j.F.T.Pittman D.S.langley Centre for Polymer Processing Simulation and Design ADOPT Research Group Departments of Mechanical and Civil Engineering University of Wales SwanseaSingleton ParkSwanseaSA2 8PPUK Centre for Polymer Processing Simulation and Design ADOPT Research Group Departments of Mechanical and Civil Engineering University of Wales SwanseaSingleton ParkSwanseaSA2 8PPUK Centre for Polymer Processing Simulation and Design ADOPT Research Group Departments of Mechanical and Civil Engineering University of Wales SwanseaSingleton ParkSwanseaSA2 8PPUK

＜正＞A brief review of the application of optimisation methods in conjunction with numerical simulation to design of extrusion dies for plastics and rubber is given. Following this, based on a simulation of slit die performance that takes into account the coupling of melt flow and die body deflection due to melt pressure, a genetic algorithm is applied to determine choker bar profiles to give optimum melt flow distribution. The software makes it possible to predict the practical operating range of dies in terms of melt flow properties and throughput.

关键词： optimization slit die design die body deflection

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营销战第1版

矿区复田恢复植被——美国北部草原露天煤矿矿区成功地恢复了草原植被

丛书名：财经易文

2002年

作者：（美）A·里斯（Al ries）（美）j·特劳特（jack Trout）李正栓贾纪芳译

本书重点阐述了市场营销战役中的四种常见常用的战略形式，如防御战、进攻战、侧翼战和游击战；针对每一种形式又提出了三条应遵循的原则，以及如何在具体的市场营销战役中应用这些原则。

ISBN: (数字)7500554338

ISBN: (纸本)7500554338

关键词：市场营销学

来源：汇雅图书（南通市图书馆... 评论

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环境科学与管理 1984年第6期 1-4+36页

作者： R、E、ries E、j、Depuit 肖剑民

近年来,美国北部草原上露天煤矿的开采急剧增加,露天煤矿剥采后,应复田建立植被,保护水土,防止侵蚀。北部草原矿区复田以种植多年生草本植物为主。在本世纪七十年代初期,当该地区的煤矿加速被开采、矿区复田刚开始的时候,我们并没有复... 详细信息

近年来,美国北部草原上露天煤矿的开采急剧增加,露天煤矿剥采后,应复田建立植被,保护水土,防止侵蚀。北部草原矿区复田以种植多年生草本植物为主。在本世纪七十年代初期,当该地区的煤矿加速被开采、矿区复田刚开始的时候,我们并没有复田种植的经验。很多地方早期的复田种植都失败了,当时人们产生了怀疑,争论复田种植能否成功然而,复田种植技术迅速地发展了,现在,先进的种植技术与管理经验使得矿区复田恢复植被的可能性大大增加了。一、自然植被北部草原的东部地区尽管气候潮湿,

关键词：草原植被兰草水牛草恢复植被露天煤矿

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美国锌公司的采矿方法

矿业工程 1964年第2期 30-31页

作者： M. j. langley 汪伯煜

美国田纳西州的锌公司目前开采的是闪锌矿。矿体很大,呈分支状。矿石在水平方向上分布,其品位和垂直厚度都很不规则。虽然在183米的垂高范围内均发现锌矿,但可采总厚度仅有76.24米,而且矿石的分布是分散的。仅少数地区的矿石是连续分布... 详细信息

美国田纳西州的锌公司目前开采的是闪锌矿。矿体很大,呈分支状。矿石在水平方向上分布,其品位和垂直厚度都很不规则。虽然在183米的垂高范围内均发现锌矿,但可采总厚度仅有76.24米,而且矿石的分布是分散的。仅少数地区的矿石是连续分布的,其厚度在45.7米以上。美国锌公司在该区有四个生产矿井,日产6350吨。其中最老的是2号矿,是从1916年

关键词：凿岩机冲击器打眼机掏槽法装岩机漏斗口装矿矿石美国美利坚合众国北美洲

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适用于采矿土地的多年生牧草

水土保持应用技术 1987年第1期 20-24页

作者： R·E·ries E·j·DePuit 刘利年

在美国北方大平原,露天煤矿在过去的十年间显著地增加.采矿后,受扰动地区的植被必须迅速恢复,以保护重建的矿区土壤剖面不受侵蚀.

关键词：暖季牧草野牛草恢复植被格兰马草草种蓝茎冰草土地冷季

Improved symptoms following bumetanide treatment in children aged 3-6 years with autism spectrum disorder:a randomized,double-blind,placebo-controlled trial

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Science Bulletin 2021年第15期66卷 1591-1598,M0004页

作者： Yuan Dai Lingli Zhang juehua Yu Xin Zhou Hua He Yiting ji Kai Wang Xiujuan Du Xin Liu Yun Tang Shining Deng Christelle langley Wei-Guang Li jun Zhang jianfeng Feng Barbara j.Sahakian Qiang Luo Fei Li Department of Developmental and Behavioural Pediatric&Child Primary Care Brain and Behavioural Research Unit of Shanghai Institute for Pediatric Research and MOE-Shanghai Key Laboratory for Children’s Environmental HealthXinhua HospitalShanghai Jiao Tong University School of MedicineShanghai 200092China Centre for Experimental Studies and Research The First Affiliated Hospital of Kunming Medical UniversityKunming 650032China Clinical Research Unit Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai 200092China The School of Psychology and Cognitive Science East China Normal UniversityShanghai 200062China Department of Psychiatry and the Behavioural and Clinical Neuroscience Institute University of CambridgeCambridge CB21TNUK Collaborative Innovation Center for Brain Science Department of Anatomy and PhysiologyShanghai Jiao Tong University School of MedicineShanghai 200025China Institute of Science and Technology for Brain-Inspired Intelligence Ministry of Education-Key Laboratory of Computational Neuroscience and Brain-Inspired Intelligence and Research and Research Institute of Intelligent Complex SystemsFudan UniversityShanghai 200433China State Key Laboratory of Medical Neurobiology and Ministry of Education Frontiers Center for Brain Science Institutes of Brain Science and National Clinical Research Center for Aging and MedicineHuashan HospitalFudan UniversityShanghai 200040China

With the current limited drug therapy for the core symptoms of autism spectrum disorder(ASD),we herein report a randomized,double-blind,placebo-controlled trial to investigate the efficacy,safety,and potential neural mechanism of bumetanide in children with ASD aged 3-6 years old.A total of 120 children were enrolled into the study and randomly assigned to either 0.5 mg bumetanide or *** the final sample,119 children received at least one dose of bumetanide(59 children) or placebo(60 children) were included in the final *** primary outcome was a reduction in the Childhood Autism Rating Scale(CARS) score,and the secondary outcomes were the Clinical Global Impressions Scale(CGI)-Global Improvement(CGI-I) score at 3 months and the change from baseline to 3-month in the Autism Diagnostic Observation Schedule(ADOS).Magnetic resonance spectroscopy(MRS) was used to measure y-aminobutyric acid(GABA) and glutamate neurotransmitter concentrations in the insular cortex(IC) before and after the *** compared with the placebo,bumetanide treatment was significantly better in reducing the *** patient withdrew from the trial due to adverse *** superiority of bumetanide to placebo in reducing insular GABA,measured using MRS,was *** clinical improvement was associated with a decrease in insular GABA in the bumetanide *** conclusion,this trial in a large group of young children with predominantly moderate and severe ASD demonstrated that bumetanide is safe and effective in improving the core symptoms of ***,the clinical significance remains uncertain,and future multi-center clinical trials are required to replicate these findings and confirm the clinical significance using a variety of outcome measures.

关键词： Autism spectrum disorder Children RCT Bumetanide GABA

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