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Preparation and incubation conditions affect the DNA integrity of ejaculated human spermatozoa

Asian Journal of Andrology 2010年第5期12卷 753-759页

作者： rieko matsuura Takumi Takeuchi Atsumi Yoshida The Reproduction Center Kiba Park Clinic Tokyo 135-0042 Japan

Appropriate semen processing and assessment are critical for successful infertility treatment. We investigated whether laboratory procedures including semen preparation and incubation affect sperm DNA integrity. A total of 153 infertile men were involved. Conventional semen parameters and sperm chromatin structure assay （SCSA） parameters, that is, DNA fragmentation index （%DFI） and high DNA stainability （%HDS）, were assessed on the flesh ejaculated semen samples, which were treated and incubated under different conditions. Negative correlations were identified between the %DFI and sperm concentration, motility, progressive motility and morphology. A lower percentage of DFI was detected in spermatozoa when density gradient centrifugation （DGC） was followed by swimup treatment in comparison with DGC alone （P 〈 0.01）. Although the %DFI increased in a time-dependent manner with incubation both at room temperature （RT） and at 37℃ in air, the %DFI after 24 h at RT was significantly lower than that at 37℃ （P 〈 0.05）. Incubation with 5% CO2 was effective in maintaining sperm motility （P 〈 0.01）; however, it induced further elevation of %DFI （P 〈 0.001）. Thus, sperm DNA damage was associated with longer incubation periods. Interestingly, common culture conditions, such as maintaining pH and temperature, compromised the sperm DNA integrity.

关键词： density gradient centrifugation DNA damage male infertility sperm chromatin structure assay spermatozoa

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Attention-Deficit/Hyperactivity Disorder in Adults with High-Functioning Pervasive Developmental Disorders in Japan

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Open Journal of Psychiatry 2014年第4期4卷 372-380页

作者： Yasuko Takanashi Hirobumi Mashiko Hirohide Yokokawa Yoko Kawasaki Shuntaro Itagaki Hiromichi Ishikawa Norihiro Miyashita Yasuaki Hayashi Asako Kudo Kentaro Oga rieko matsuura Shin-Ichi Niwa Department of Neuropsychiatry Fukushima Medical University School of Medicine Fukushima Japan Fukushima General Health and Welfare Center Fukushima Japan Department of Public Health Fukushima Medical University School of Medicine Fukushima Japan Department of General Medicine Juntendo University School of Medicine Tokyo Japan Musasino Child Development Clinic Tokyo Japan Hanawakousei Hospital Fukushima Japan Landic Nihonbashi Clinic Tokyo Japan Hoshigaoka Hospital Fukushima Japan Surugadai Nihon University Hospital Tokyo Japan Shiba Clinic Tokyo Japan

Aims: This study was designed to verify the proportion of Japanese adults with pervasive developmental disorder (PDD) who met the diagnostic criteria (other than E) for attention-deficit/hyperactivity disorder (ADHD) in the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, Text Revision (DSM-IV-TR). Furthermore, we examined to what extent adults with PDD think that they exhibit ADHD symptoms. Methods: We developed an original Japanese self-report questionnaire to determine the presence or absence of 18 symptoms from the diagnostic criteria for ADHD in the DSM-IV-TR. We administered the questionnaire to 64 adults with high-functioning PDD (45 men and 19 women) and 21 adults with ADHD (10 men and 11 women), aged 18 to 59 years, with a full-scale intelligence quotient ≥75. Target patients were evaluated for ADHD by their psychiatrists. Results: Twenty-nine (45.3%) adults with PDD also had ADHD. The percentage of these adults who had over six perceived inattention symptoms from the DSM-IV-TR was 96.6%. The percentage of these adults who had over six perceived hyperactivity-impulsivity symptoms was 65.5%. Thirty-five (55.6%) adults with PDD responded that they were aware of having ADHD symptoms at the level of the relevant diagnostic criteria. Conclusions: The present study is the first to examine the frequency of objective and perceived ADHD symptoms in adults with PDD in Japan. Our results show that both objective and perceived ADHD symptoms frequently appear in a large number of adults with PDD. This suggests that it is necessary to attend to concomitant ADHD symptoms in the medical care of adults with PDD.

关键词： Adults Attention-Deficit/Hyperactivity Disorder (ADHD) High-Functioning Pervasive Developmental Disorders (PDD) Self-Report

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