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World Journal of Vaccines 2014年第4期4卷 164-174页

作者： Toshiaki Shimoyama Takumi Kubota Junsuke Shirai rie watanabe Laboratory of Epizootiology Cooperative Department of Veterinary Medicine Faculty of Agriculture Tokyo University of Agriculture and Technology Fuchu Japan Research and Education Center for Prevention of Global Infectious Disease of Animals Fuchu Japan Veterinary Hygiene Joint Faculty of Veterinary Medicine Yamaguchi University Yamaguchi Japan

Four capsid proteins (VP1, VP2, VP3, and VP4) of coxsackievirus B3 (CVB3) were expressed as recombinant proteins in an Escherichia coli expression system and used as antigens for subunit vaccines against CVB3 in ICR mice. Antigens were expressed as thioredoxin-histidine (TrxHis)-tagged protein and purified before immunization. Although all VPs other than VP4 induced anti-CVB3 specific antibodies in mice (detected by ELISA and western blotting), they did not neutralize the infectious CVB3 in a virus neutralization assay. Meanwhile, 2 virus strains were purified from CVB3 virus stock on the basis of their plaque size on HeLa cells. ICR mice were infected with the 2 purified virus strains (S-strain and L-strain) and unpurified virus stock (wild type) to analyze the difference in antibody responses against infections of purified and unpurified virus strains. The reactivity of antisera against each virus strain was tested by ELISA, and the results showed that the inoculation of purified virus strain induced a strong antibody response against the inoculated strain. As a result, the antibody response against wild-type and other virus strains was suppressed. These results suggest using unpurified virus stock as an antigen is advantageous for inducing a broad antibody response in inoculated animals.

关键词： Coxsackievirus B3 Antibody Response Cross Reactivity

Effects of silicon addition on optical properties of mould flux with iron oxide and estimation of apparent thermal conductivity of flux

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Journal of Iron and Steel Research International 2019年第4期26卷 365-373页

作者： rie Endo Yo Kan Takashi watanabe Miyuki Hayashi Masahiro Susa Department of Materials Science and Engineering Tokyo Institute of TechnologyTokyo 152-8552Japan Department of Metallurgy and Ceramics Science Tokyo Institute of TechnologyTokyo 152-8552Japan

Mould flux with high absorptivity had been obtained in the reduction process of mould flux by *** further utilisation of the mould flux with high absorptivity,the effect of silicon addition on the optical property has been *** synthesised mould flux powder with iron oxide was melted with the addition of silicon powder at 1703 K followed by quenching,thus forming a glassy *** 5-30-min reduction,the samples exhibited black opaque ***,the samples that were reduced for 45-60 min had a bluish grey *** iron-based particles dispersed in the samples were micrometre order in *** determine the effect of iron particles on the optical properties of the sample,extinction,scattering and absorption efficiencies were calculated according to Mie *** showed that both scattering on the iron particle and absorption by iron effectively increase the extinction efficiency in the mould ***,the silicon particle in the flux has large scattering *** black opaque colour is contributed by the existence of silicon and iron *** apparent thermal conductivity was calculated for the solid *** also indicated that the entire glassy mould flux would effectively work for mild cooling in a continuous casting process owing to high absorptivity and small thermal conductivity.

关键词： Mould flux Reduction Iron oxide Thermal conductivity Optical property

Preoperative diagnosis of gastrointestinal stromal tumor by endoscopic ultrasound-guided fine needle aspiration

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World Journal of Gastroenterology 2007年第14期13卷 2077-2082页

作者： Kazuya Akahoshi Yorinobu Sumida Noriaki Matsui Masafumi Oya rie Akinaga Masaru Kubokawa Yasuaki Motomura Kuniomi Honda Masayuki watanabe Takashi Nagaie Department of Gastroenterology Aso Iizuka Hospital Iizuka 820-8505Japan Department of Pathology Aso Iizuka Hospital Iizuka 820-8505Japan Department of Surgery Aso Iizuka Hospital Iizuka820-8505Japan

AIM： to evaluate the role of endoscopic ultrasonographyguided fine needle aspiration （EUS-FNA） in the preoperative diagnosis of gastrointestinal stromal tumor （GIST）.METHODS： From September 2002 to June 2006, Fiftythree consecutive EUS-FNAs of GI tract subepithelial hypoechoic tumors with continuity to proper muscle layer suspected as GIST by standard EUS were evaluated prospectively. The reference standards for the final diagnosis were surgery （n = 31）, or clinical follow-up （n = 22）. Additionally, immunophenotyping of specimens obtained by EUS-FNA and surgical resection specimens were compared.RESULTS： In 2 cases puncture was not performed because of anatomical problems. The collection rate of adequate specimens from the GI tract subepithelial hypoechoic tumor with continuity to proper muscle layer was 82% （42/51）. The diagnostic rate for the tumor less than 2 cm, 2 to 4 cm, and 4 cm or more were 71% （15/21）, 86% （18/21）, and 100% （9/9）,respectively. In 29 surgically resected cases, the sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy of EUS-FNA using immunohistochemical analysis of GIST were 100%（24/24）, 80% （4/5）, 96% （24/25）, 100% （4/4）, and 97% （28/29）, respectively. No major complications were encountered.CONCLUSION： EUS-FNA with immunohistochemical analysis is a safe and accurate method in the pretherapeutic diagnosis of GIST. It should be taken into consideration in decision making, especially in early diagnosis following minimal invasive surgery for GIST.

关键词： Gastrointestinal stromal tumor Ultrasound-guided fine needle aspiration Immunohistochemicalanalysis

Utility of FibroScan-based scoring systems to narrow the risk group of nonalcoholic fatty liver disease with comorbidities

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World Journal of Gastrointestinal Pathophysiology 2022年第3期13卷 96-106页

作者： Kouichi Miura Hiroshi Maeda Naoki Morimoto Shunji watanabe Mamiko Tsukui Yoshinari Takaoka Hiroaki Nomoto rie Goka Kazuhiko Kotani Hironori Yamamoto Department of Medicine Division of GastroenterologyJichi Medical University School of Medicine3311-1 YakushijiShimotsuke 329-0498TochigiJapan Division of Community and Family Medicine Center for Community MedicineJichi Medical University3311-1 YakushijiShimotsuke 329-0498Japan

BACKGROUND Vibration-controlled transient elastography(VCTE)is proposed as a second step of examination to assess liver fibrosis in patients with nonalcoholic fatty liver disease(NAFLD)after triaging by the fibrosis-4(FIB-4)***,VCTEbased scoring systems,including FibroScan-AST(FAST),Agile 3+,and Agile 4,emerged to determine the status of ***,the significance of these scoring systems remains unknown in narrowing the high-risk group of NAFLD patients with comorbidities,including hepatocellular carcinoma(HCC)and esophagogastric varices(EGV).AIM To clarify the significance of VCTE-based scoring systems to narrow the high-risk group of NAFLD patients with *** We performed a cross-sectional study to investigate the usefulness of VCTE-based scoring systems and other fibrosis markers to narrow the high-risk group of patients with ***-4 index was used for the first *** groups of FAST,Agile 3+,and Agile 4 were stratified according to the published *** the 191 patients with NAFLD,there were 26(14%)and 25 patients(13%)with HCC and EGV,*** When 1.3 was used as a cutoff value,the FIB-4 index narrowed the risk group to 120 patients,in which all patients with HCC and/or EGV were *** risk group of Agile 3+could subsequently narrow the risk *** prevalence of HCC and EGV at this step were 33%(26/80)and 31%(25/80),*** further narrowing of EGV,Agile 4 aggregated the patients with EGV into 43 patients,of whom 23(53%)had *** failed to narrow the risk group of patients with *** 2.6 was used as a cutoff value of the FIB-4 index,three patients with HCC and two patients with EGV were missed at the first *** Agile 3+and Agile 4 are useful to narrow the NAFLD patient group,in which patients may have HCC and/or EGV.

关键词： Nonalcoholic fatty liver disease Vibration controlled transient elastography Non-invasive test Hepatocellular carcinoma Varix

Usefulness of Fractional Exhaled Nitric Oxide-Guided Treatment in Patients with Asthma-Chronic Obstructive Pulmonary Disease Overlap

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Open Journal of Respiratory Diseases 2018年第1期8卷 1-12页

作者： Taisuke Akamatsu Toshihiro Shirai Yuko Tanaka Hirofumi watanabe Yoshinari Endo Yukiko Shimoda Takahito Suzuki rie Noguchi Mika Saigusa Akito Yamamoto Yuichiro Shishido Takefumi Akita Satoru Morita Kazuhiro Asada Department of Respiratory Medicine Shizuoka General Hospital Shizuoka Japan

Background: Some patients present clinical features of both asthma and chronic obstructive pulmonary disease (COPD), which has led to the recent proposal of asthma-COPD overlap (ACO) as a diagnosis. Fractional exhaled nitric oxide (FeNO) is a candidate biomarker to diagnose ACO. We assessed the effect of an add-on treatment with budesonide/formoterol (BUD/FM) combination in patients with ACO, which was diagnosed by FeNO. Methods: This was a prospective, single-arm, open-label, before and after comparison study. Subjects included 83 patients with COPD who attended outpatient clinics for routine checkups at Shizuoka General Hospital between June and November 2016. All patients fulfilled the GOLD definition of COPD and were receiving long-acting muscarinic antagonist (LAMA) or LAMA/long-acting β2 agonist (LABA) combinations. After an 8-week run-in period, BUD/FM was added to the patients with FeNO levels of ≥35 ppb, defined as having ACO. For patients receiving LAMA/LABA, BUD/FM was added after the discontinuation of LABA. The modified British Medical Research Council (mMRC) score, COPD assessment test (CAT) score, spirometric indices, forced oscillation parameters, and FeNO were assessed before and after 8 weeks of BUD/ FM add-on treatment. Results: Twenty-four patients (28.9%) had FeNO levels ≥ 35 ppb, and 17 patients completed the study (mean age: 73 years and GOLD I/II/III/IV, 5/10/1/1). The mean CAT scores significantly improved (9.2 to 5.4, p = 0.015) and 10 patients (58.8%) showed ≥2 points improvement, a minimal clinically important difference. The mean FeNO levels significantly decreased from 63.0 to 34.3 ppb (p Conclusions: FeNO-guided treatment with BUD/FM improves symptoms in patients with ACO.

关键词： Asthma-COPD Overlap Budesonide/Formoterol Combination COPD Assessment Test Fractional Exhaled Nitric Oxide

Motion reconstruction of the small carry-on impactor aboard Hayabusa2

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Astrodynamics 2020年第4期4卷 289-308页

作者： Takanao Saiki Yuya Mimasu Yuto Takei Manabu Yamada Hirotaka Sawada K azunori Ogawa Naoko Ogawa Hiroshi Takeuchi Akira Miura Yuri Shimaki Koji Wada rie Honda Yasuhiro Yokota Kei Shirai Naruhisa Sano Hirohito Ohtsuka Go Ono Kent Yoshikawa Shota Kikuchi Chikako Hirose Yukio Yamamoto Takahiro Iwata Masahiko Arakawa Seiji Sugita Satoshi Tanaka Fuyuto Terui Makoto Yoshikawa Satoru Nakazawa Sei-ichiro watanabe Yuichi Tsuda Japan Aerospace Exploration Agency Sagamihara 252-5210Japan Chiba Institute of Technology Narashino 275-0016Japan Kobe University K obe 657-8501Japan The Graduate University for Aduanced Studies Sagamihara 252-5210Japan Kochi University Kochi 780-8520Japan IHI Aerospace Co. LtdTomioka 370-2398Japan The University of Tokyo Tokyo 113-0033Japan Nagoya University Nagoya 464-8601Japan

Subsurface exploration is one of the most ambitious scientific objectives of the Hayabusa2 mission.A small device called small carry-on impactor(SCI)was developed to create an artificial crater on the surface of asteroid *** enables us to sample subsurface materials,which will provide a window to the *** physical properties of the resulting crater are also useful for understanding the internal structure of *** understanding of the crater and ejecta properties,including the depth of excavation of subsurface materials,requires accurate information on impact *** particular,the impact angle is a critical factor because it greatly influences the size and shape of the *** April 5,2019,the Hayabusa2 spacecraft deployed the SCI at 500 m of altitude above the asteroid *** SCI gradually reduced its altitude,and it shot a 2 kg copper projectile into the asteroid 40 min after *** the position of the released SCI is essential for determining the impact *** study describes the motion reconstruction of the SCI based on the actual operation *** results indicate that the SCI was released with high accuracy.

关键词： small body exploration Hayabusa2 kinetic impact artificial crater motion reconstruction