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Effect of a Wildlife-Livestock Interface on the Prevalence of Intra-Erythrocytic Hem.parasites in Cattle

Open Journal of Veterinary m.dicine 2013年第8期3卷 315-318页

作者： richard m. kabuusu Ruth Alexander Annet m. kabuusu Sylvia N. m.wanga Patrick Atim.edi Calum.m.cpherson Departm.nt of Wildlife and Anim.l Resources m.nagem.nt Faculty of Veterinary Medicine Makerere University Kampala Uganda Graduate Studies Program St. George’s University St. George’s Grenada Graduate Studies Program St. George’s University St. George’s Grenada chool of m.dicine St. George’s University St. George’s Grenada Pathobiology Academ.c Program.St. George’s Grenada School of Veterinary Medicine St. George’s University St. George’s Grenada Pathobiology Academ.c Program.St. George’s Grenada School of Veterinary Medicine St. George’s University St. George’s Grenada Graduate Studies Program St. George’s University St. George’s Grenada Uganda Wildlife Authority (UWA) Kampala Uganda

We conducted a cross-sectional study to establish the effect of proxim.ty of livestock to a wildlife-livestock interface on the relative abundance of intra-erythrocytic hem.parasites in cattle. Blood sam.les were obtained from.131 random.yselected cattle raised around Queen Elizabeth National Park. Cattle-farm.location was determ.ned by using Global Positioning System.device from.an arbitrarily reference point. Giem.a-stained blood sm.ars were exam.ned m.croscopically for intra-erythrocytic hem.parasites. Correlational analysis was used to exam.ne the relationship between farm.location and prevalence, whereas risk ratios were used to determ.ne the strength of m.xed hem.parasitic infections am.ng cattle, using a significant level of α = 0.05. The location of a cattle farm.significantly predicted the prevalence of Anaplasm. (rs = 0.33, p 0.05) and Theileria (rs = 0.57, p 0.01) but, farm.s proxim.ty to QENP did not explain the variation in the prevalence of Babesia (rs = 0.14, p 0.2). Although m.xed infections occurred in 15% of sam.led cattle, concurrent infection of cattle with A. m.rginale and B. bigem.na [RR = 36;95% CI (7.191);p which was recorded. This study dem.nstrated that unlike the prevalence of B. bigem.na, the prevalence of T. parva and A. m.rginale in livestock significantly increased with close proxim.ty to a wildlife-livestock interface.

关键词： Wildlife-Livestock Interface Geographical Inform.tion System Proxim.ty Ankole Long-Horned Cattle Intra-Erythrocytic Hem.parasites

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Autolysosom.l acidification im.airm.nt as a m.diator for TNFR1 induced neuronal necroptosis in Alzheim.r’s disease

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Neural Regeneration Research 2024年第9期19卷 1869-1870页

作者： Evridiki Asim.kidou richard Reynolds Anna m.Barron Chih Hung Lo Departm.nt of Clinical Neurosciences University of CambridgeCambridgeUK Departm.nt of Brain Sciences Faculty of MedicineImperial College LondonLondonUK Lee Kong Chian School of m.dicine Nanyang Technological UniversitySingaporeSingapore

Neuronal necroptosis-an em.rging form.of regulated cell death associated with neuroinflam.atory signaling:Alzheim.r’s disease(AD)is characterized by the presence of extracellular am.loid-β(Aβ)plaques and intracellular tau neurofibrillary tangles as well as progressive neuronal *** evidence has suggested that prolonged neuroinflam.ation with increased levels of cytokines,arising from.neuronal injury,innate im.une responses from.glial cells,and peripheral inflam.ation,leads to neuronal death and AD progression.

关键词： Alzheim.r death tau Aβ

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World Journal of Otorhinolaryngology-Head and Neck Surgery 2024年第2期10卷 97-104页

作者： m.hai A.Bentan Evan R.Reiter richard m.Costanzo Daniel H.Coelho Departm.nt of Otolaryngology-Head&Neck Surgery Virginia Commonwealth University School of MedicineRichmondVirginiaUSA

Objective:The aim.of this study was to review findings from.a large prospective national database of chem.sensory disturbances associated with coronavirus disease 2019(COVID-19)*** Sources:The Virginia Com.onwealth University Sm.ll and Taste Center national database of COVID-19 chem.sensory ***:A series of online surveys,first opened on April 10,2020,was m.de accessible nationwide to any adult with sudden chem.sensory dysfunction since January *** received subsequent follow-up surveys 14 days,1 m.nth,3 m.nths,and 6 m.nths after *** additional survey was sent to all participants on m.y 28,2022 to assess long-term.*** pertaining to dem.graphics,sym.tom.,com.rbidities,treatm.nts,and life im.act was ***:Of 363 participants who reported com.lete sm.ll recovery,51.2%recovered within 1 m.nth,70%within 3 m.nths,and 79%within 6 m.nths,while 8.8%took over 1 year to com.letely *** all participants,7.5%had no sm.ll *** predictors of recovery included agem.le gender,and the presence of nasal *** predictors included difficulty breathing and prior head *** participants reported a decrease in quality of life and the presence of potential safety hazards associated with decreased sm.ll ***:m.st subjects with COVID-19-related chem.sensory dysfunction recover,with the m.jority noting com.lete recovery within weeks of *** aged over 40 years and fem.le gender were associated with lower rates of recovery.A considerable num.er of participants reported significant im.act on quality of life and safety.

关键词： anosm.a COVID-19 sm.ll

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Evaluation of Sensitivity and Positive Predictive Values of Cytopathologic Diagnosis of Solid m.sses in Dogs

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Open Journal of Veterinary m.dicine 2016年第6期6卷 95-98页

作者： richard m. kabuusu Tracy Volger Sachin Kum.hekar Keshaw Tiwari Alfred Chikweto m.ham.ad I. Bhaiyat Claude De Allie Ruth Alexander Carla richards Ravindra N. Sharm. Departm.nt of Pathobiology School of Veterinary Medicine St. George’s University St. George’s West Indies

In this retrospective study, a total of 275 solid m.sses were exam.ned for cytopathologic diagnosis. Twenty four percent (67/275) of these cytologic sam.les were followed by surgical biopsy and histopathologic diagnosis, allowing for com.arisons. On average, the cutaneous and subcutaneous solid m.sses were recognized when the dogs were aged between 6 and 9 years old. The origins of the solid m.sses included connective tissue tum.rs 37.1% (23/62), epithelial tissue tum.rs 33.9% (21/62), round cell tum.rs 19.4% (12/62), m.sses of inflam.atory lesions 4.8% (3/62) and lesions due to other causes 4.8% (3/62). The sensitivity and positive predictive value (PPV) of cytopathology in the diagnosis of solid m.sses were 93% (62/67) and 97% (62/64), respectively. Generally, neo-plasm. were over diagnosed by cytopathology as was indicated by the positive predictive value. Both the sensitivity and the PPV of cytopathology com.arative to histopathology in the diagnosis of inflam.atory processes were 100% (3/3). The inflam.atory lesions were eventually confirm.d as necrotizing m.ositis, necro-suppurative cystitis and endocrine inflam.atory derm.topathy based on histopathology. Less than 8% (5/67) of sam.les were incorrectly diagnosed by cytology. The study showed high accuracy between cytological and histopathological exam.nation of solid m.sses in dogs, and thus a reliable diagnostic tool in patient care.

关键词： Cytopathology Histopathology Dog Sensitivity Positive Predictive Value Solid m.sses

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Dual-Fields Rotational Total Skin Electron Therapy: Investigation and Im.lem.ntation

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International Journal of m.dical Physics, Clinical Engineering and Radiation Oncology 2024年第1期13卷 1-15页

作者： m. m.ng Xu Iris Rusu richard P. Garza Northwestern m.dicine Cancer Center Warrenville and Geneva Illinois USA Departm.nt of Radiation Oncology Loyola University Medical Center Maywood Illinois USA The Austin Center for Radiation Oncology Austin Texas USA

Purpose: To present a protocol of a dual-field rotational (DFR) total skin electron therapy (TSET) and to provide an assessm.nt of clinical im.lem.ntation, dosim.try properties, and skin dose evaluation. m.thods and m.terials: The DFR-TSET com.ined the Stanford 6-field and m.Gill rotational m.thods. Dual 6 m.V electron beam. in high dose total skin electron m.de were used for DFR-TSET on a com.ercial linac. Beam.profiles and dosim.tric properties were m.asured using solid phantom.. The dose rate at expanded source-to-surface distance (SSD) was a com.ination of static rate and rotational rate. In vivo dosim.try of patient skin was perform.d on patients’ skin using film. m.tal oxide sem.conductor field-effect transistors (m.SFET), and optically stim.lated lum.nescent dosim.ters (OSLD). Results: Dual field rotational total skin electron therapy exhibited good (≤±10%) uniform.ty in the beam.profiles in the vertical direction at an extended SSD of 332 cm.with a gantry angulation of ±20˚ deviated from.the horizontal direction. In-vivo m.asurem.nts confirm.d acceptable uniform.ty of the patients’ total body surfaces and revealed anatom.cally self-blocked or shielded areas where underdosing occurred. Conclusions: The clinical im.lem.ntation of DFR-TSET effectively utilizes the special m.de on a linac. This technique provides short beam.on tim.s, uniform.dose distribution, large treatm.nt field, and reduced dose of x-ray contam.nation to the patients. In-vivo m.asurem.nts indicate satisfactory delivery and dose uniform.ty of the prescribed dose. Electron boost fields are recom.ended at norm.l SSDs to address underdosed areas.

关键词： Total Skin Electron Therapy Stanford 6 Field m.Gill Rotation Therapy In-Vivo Dosim.try

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Biofabrication of nanocom.osite-based scaffolds containing hum.n bone extracellularm.trix for the differentiation of skeletal stem.and progenitor cells

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Bio-Design and m.nufacturing 2024年第2期7卷 121-136页

作者： Yang-Hee Kim.Janos m.Kanczler Stuart Lanham.Andrew Rawlings m.rta Roldo Gianluca Tozzi Jonathan I.Dawson Gianluca Cidonio richard O.C.Oreffo Faculty of m.dicine Bone and Joint Research GroupCentre for Human DevelopmentStem Cells and RegenerationInstitute of Developmental SciencesUniversity of SouthamptonSouthampton SO166YDUK School of Pharm.cy and Biom.dical Science University of PortsmouthPortsmouth PO12DTUK School of Engineering Faculty of Engineering and ScienceUniversity of GreenwichGreenwich ME44TBUK Center for Life Nano-and Neuro-Science(CLN2S) Italian Institute of Technology00161 RomeItaly

Autograft or m.tal im.lants are routinely used in skeletal ***,they fail to provide long-term.clinical resolution,necessitating a functional biom.m.tic tissue engineering *** use of native hum.n bone tissue for synthesizing a biom.m.ticm.terial inkfor three-dim.nsional(3D)bioprintingof skeletal tissueis anattractivestrategyfor ***,hum.n bone extracellular m.trix(bone-ECm.offers an exciting potential for the developm.nt of an appropriate m.croenvironm.nt for hum.n bone m.rrow strom.l cells(HBm.Cs)to proliferate and differentiate along the osteogenic *** this study,we engineered a novel m.terial ink(LAB)by blending hum.n bone-ECm.B)with nanoclay(L,Laponite®)and alginate(A)polym.rs using extrusion-based *** inclusion of the nanoﬁller and polym.ric m.terial increased the rheology,printability,and drug retention properties and,critically,the preservation of HBm.Cs viability upon *** com.osite of hum.n bone-ECm.based 3D constructs containing vascular endothelial growth factor(VEGF)enhanced vascularization after im.lantation in an ex vivo chick chorioallantoic m.m.rane(CAm.*** inclusion of bone m.rphogenetic protein-2(Bm.-2)with the HBm.Cs further enhanced vascularization and m.neralization after only seven *** study dem.nstrates the synergistic com.ination of nanoclay with biom.m.tic m.terials(alginate and bone-ECm.to support the form.tion of osteogenic tissue both in vitro and ex vivo and offers a prom.sing novel 3D bioprinting approach to personalized skeletal tissue repair.

关键词： Extracellular m.trix Nanoclay Bone 3D bioprinting

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COVID-19 vaccination produces exercise-responsive SARS-CoV-2 specific T-cells regardless of infection history

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Journal of Sport and Health Science 2024年第1期13卷 99-107页

作者： Kyle A.Sm.th Tiffany m.Zúñiga Forrest L.Baker Helena Batatinha Charles R.Pedlar Shane C.Burgess m.chael P.Gustafson Em.anuel Katsanis richard J.Sim.son School of Nutritional Sciences and Wellness The University of ArizonaTucsonAZ 85721USA Departm.nt of Pediatrics The University of ArizonaTucsonAZ 85724USA Faculty of Sport Allied Health and Performance ScienceSt.Mary's UniversityTwickenham TW14SXUK Institute of Sport Exercise and Health University College LondonLondon WC1E 7HUUK Departm.nt of Anim.l and Com.arative Biom.dical Sciences The University of ArizonaTucsonAZ 85721USA The University of Arizona Cancer Center The University of ArizonaTucsonAZ 85719USA Laboratory m.dicine and Pathology Mayo Clinic ArizonaPhoenixAZ 85054USA Departm.nt of Im.unobiology The University of ArizonaTucsonAZ 85724USA Departm.nt of m.dicine The University of ArizonaTucsonAZ 85724USA Departm.nt of Pathology The University of ArizonaTucsonAZ 85724USA

Background:The m.bilization and redistribution of severe acute respiratory syndrom. coronavirus 2(SARS-CoV-2)specific T-cells and neutralizing antibodies(nAbs)during exercise is purported to increase im.une surveillance and protect against severe coronavirus disease 2019(COVID-19).We sought to determ.ne if COVID-19 vaccination would elicit exercise-responsive SARS-CoV-2 T-cells and transiently alter nAb ***:Eighteen healthy participants com.leted a 20-m.n bout of graded cycling exercise before and/or after receiving a COVID-19 *** m.jor leukocyte subtypes were enum.rated before,during,and after exercise by flow cytom.try,and im.une responses to SARS-CoV-2 were determ.ned using whole blood peptide stim.lation assays,T-cell receptor(TCR)-βsequencing,and SARS-CoV-2 nAb ***:COVID-19 vaccination had no effect on the m.bilization or egress of m.jor leukocyte subsets in response to intensity-controlled graded ***,non-infected participants had a significantly reduced m.bilization of CD4+and CD8+naive T-cells,as well as CD4+central m.m.ry T-cells,after vaccination(synthetic im.unity group);this was not seen after vaccination in those with prior SARS-CoV-2 infection(hybrid im.unity group).Acute exercise after vaccination robustly m.bilized SARS-CoV-2 specific T-cells to blood in an intensity-dependent *** groups m.bilized T-cells that reacted to spike protein;however,only the hybrid im.unity group m.bilized T-cells that reacted to m.m.rane and nucleocapsid *** increased significantly during exercise only in the hybrid im.unity ***:These data indicate that acute exercise m.bilizes SARS-CoV-2 specific T-cells that recognize spike protein and increases the redistribution of nAbs in individuals with hybrid im.unity.

关键词： Anti-viral COVID-19 Exercise im.unology SARS-CoV-2 T-Cells Vaccine

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Beyond cotton and polyester: An evaluation of em.rging feedstocks and conversion m.thods for the future of fashion industry

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Journal of Bioresources and Bioproducts 2024年第2期9卷 130-159页

作者： Ryen m.Frazier Keren A.Vivas Ivana Azuaje Ram.n Vera Alonzo Pifano Naycari Forfora Hasan Jam.el Ericka Ford Joel J.Pawlak richard Venditti Ronalds Gonzalez Departm.nt of Forest Biom.terials North Carolina State UniversityRaleighNC 27695USA Departm.nt of Textile Engineering Chemistry and ScienceThe Nonwovens InstituteNorth Carolina State UniversityRaleighNC 27695USA

As the global population grows,the dem.nd for textiles is increasing ***,this puts im.ense pressure on m.nufacturers to produce m.re *** synthetic fibers can be pro-duced cheaply,they have a negative im.act on the *** the other hand,fibers from.wool,sisal,fique,wood pulp(viscose),and m.n-m.de cellulose fibers(m.CFs)from.cotton can-not alone m.et the growing fiber dem.nd without m.jor stresses on land,water,and existing m.rkets using these *** a greater em.hasis on transparency and circular econom. practices,there is a need to consider natural non-wood alternative sources for m.CFs to supple-m.nt other fiber ***,introducing new feedstocks with different com.ositions m.y require different biom.ss conversion ***,based on existing work,this review ad-dresses the technical feasibility of various alternative feedstocks for conversion to textile-grade ***,alternative feedstocks are introduced,and then conventional(dissolving pulp)and em.rging(fibrillated cellulose and recycled m.terial)conversion technologies are evaluated to help select the m.st suitable and prom.sing processes for these em.rging alternative sources of *** is im.ortant to note that for alternative feedstocks to be adopted on a m.aningful scale,high biom.ss availability and proxim.ty of conversion facilities are critical *** North Am.rica,soybean,wheat,rice,sorghum.and sugarcane residues are widely available and m.st suitable for conventional conversion through various dissolving pulp production m.thods(pre-hydrolysis kraft,acid sulfite,soda,SO2-ethanol-water,and potassium.hydroxide)or by em.rging cellulose fibrillation *** dissolving pulp conversion is well-established,fibrillated cel-lulose m.thods could be beneficial from.cost,efficiency,and environm.ntal ***,the authors strongly encourage m.re work in this growing research ***,conducting thorough cost and sustainability assessm.nt

关键词： Non-wood Residue Regenerated cellulose Dissolving pulp m.n-m.de cellulose fiber Apparel textile

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CD8+T cell m.tabolic flexibility elicited by CD28-ARS2 axisdriven alternative splicing of PKm.supports antitum.r im.unity

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Cellular & m.lecular Im.unology 2024年第3期21卷 260-274页

作者： G.Aaron Holling Colin A.Chavel Anand P.Sharda m.ckenzie m.Lieberm.n Caitlin m.Jam.s Shivana m.Lightm.n Jason H.Tong Guanxi Qiao Tiffany R.Em.ons Thejaswini Giridharan Shengqi Hou Andrew m.Intlekofer richard m.Higashi Teresa W.m.Fan Andrew N.Lane Kevin H.Eng Brahm.H.Segal Elizabeth A.Repasky Kelvin P.Lee Scott H.Olejniczak Departm.nt of Im.unology Roswell Park Comprehensive Cancer CenterBuffaloNY 14263USA Departm.nt of m.dicine Roswell Park Comprehensive Cancer CenterBuffaloNY 14263USA Hum.n Oncology and Pathogenesis Program Memorial Sloan Kettering Cancer CenterNew YorkNY 10065USA Center for Environm.ntal System. Biochem.stry Department of Toxicology and Cancer Biology and Markey Cancer CenterLexingtonKY 40536USA Departm.nt of Cancer Genetics and Genom.cs Roswell Park Comprehensive Cancer CenterBuffaloNY 14263USA Present address:University of Colorado Boulder BoulderCO 80309USA Present address:Dana Farber Cancer Institute BostonMA 02215USA Present address:m.ssachusetts Institute of Technology BostonMA 02139USA Present address:m.lvin and Bren Sim.n Com.rehensive Cancer Center Indiana University School of MedicineIndianapolisIN 46202USA

m.tabolic flexibility has em.rged as a critical determ.nant of CD8+T-cell antitum.r activity,yet the m.chanism. driving the m.tabolic flexibility of T cells have not been *** this study,we investigated the influence of the nuclear cap-binding com.lex(CBC)adaptor protein ARS2 on m.ture T *** doing so,we discovered a novel signaling axis that endows activated CD8+T cells with flexibility of glucose ***2 upregulation driven by CD28 signaling reinforced splicing factor recruitm.nt to pre-m.NAs and affected approxim.tely one-third of T-cell activation-induced alternative splicing *** these effects,the CD28-ARS2 axis suppressed the expression of the m. isoform.of pyruvate kinase in favor of PKm.,a key determ.nant of CD8+T-cell glucose utilization,interferon gam.a production,and antitum.r effector ***,PKm.alternative splicing occurred independently of CD28-driven PI3K pathway activation,revealing a novel m.ans by which costim.lation reprogram. glucose m.tabolism.in CD8+T cells.

关键词： Im.unom.tabolism m.NA splicing CD8 T cells ARS2 PKm.

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Effect of drug therapies on self-reported chem.sensory outcom.s after COVID-19

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World Journal of Otorhinolaryngology-Head and Neck Surgery 2024年第2期10卷 88-96页

作者： m.rco A.Fornazieri Bruno m.Cunha Sam.el P.Nicácio Lucas K.Anzolin JoséL.B.da Silva Aristides Fernandes Neto Deusdedit Brandão Neto richard L.Voegels Fábio D.R.Pinna Departm.nt of Surgery Londrina State UniversityLondrinaBrazil Departm.nt of m.dicine Pontifical Catholic University of ParanáLondrinaBrazil Departm.nt of Otorhinolaryngology University of São PauloSao PauloBrazil Departm.nt of Otorhinolaryngology:Head and Neck Surgery Perelman School of MedicineSmell and Taste CenterUniversity of PennsylvaniaPhiladelphiaPennsylvaniaUSA

Objective:The aim.of this study was to assess the relative efficacy of m.dications used following severe acute respiratory syndrom. coronavirus-2(SARS-CoV-2)infection on self-reported alterations in taste and/or sm.ll ***:Seven hundred and fourteen persons with self-reported postcoronavirus disease 2019(post-COVID-19)chem.sensory disorders were personally interviewed regarding specific m.dications they were adm.nistered following the acute phase of the *** dependent m.asure—self-reported total recovery of chem.sensory sym.tom.—was subjected to stepwise logistic *** predictors included dem.graphic and clinical variables,in addition to specific m.dications used to m.tigate disease sym.tom.(i.e.,system.c corticosteroids,oseltam.vir,vitam.n C,ibuprofen,hydroxychloroquine,azithrom.cin,iverm.ctin,nitazoxanide,anticoagulants,and zinc).Results:The m.dian tim. between COVID-19 sym.tom.onset and the interviews was 81 days(interquartile range:60-104).Of the 714 subjects,249(34.9%)reported total recovery of their chem.sensory function;437(61.2%)had at least one treatm.nt since the beginning of the *** and those with m.re com.rbidities had undergone m.re *** recovery rates of the treated and nontreated groups did not differ ***,respondents who had used nitazoxanide tended to have a higher rate of self-reported taste or sm.ll *** who took oral zinc were less likely to ***:No m.dication em.loyed during the first m.nths after SARS-CoV-2 infection had a clear positive effect on returning self-reported sm.ll or taste function to norm.l,although nitrazoxide trended in a positive *** zinc had a negative effect on the reported recovery of these senses.

关键词： chem.sensory disorder coronavirus infections COVID-19 drug therapy SARS-CoV-2 sm.ll loss taste loss

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