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蝉花提取物通过促进Hela细胞氧化胁迫应答抑制H_2o_2诱导的细胞衰老

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四川大学学报（自然科学版） 2018年第3期55卷 632-636页

作者：王为岩高雪洁刘冰花杨力闫孟力刘科四川大学生命科学学院生物资源与生物环境重点实验室成都610065 成都大学医学院生物化学与分子生物学教研室成都610106

本实验探究蝉花提取物(CCE)通过促进Hela细胞的氧化胁迫应答抑制H_2o_2诱导的细胞衰老.在实验中,使用不同浓度的CCE处理Hela细胞48h或72h,检测细胞的存活率.然后使用H_2o_2在Hela细胞中诱导氧化胁迫,检测CCE处理组和对照组细胞的β-半... 详细信息

本实验探究蝉花提取物(CCE)通过促进Hela细胞的氧化胁迫应答抑制H_2o_2诱导的细胞衰老.在实验中,使用不同浓度的CCE处理Hela细胞48h或72h,检测细胞的存活率.然后使用H_2o_2在Hela细胞中诱导氧化胁迫,检测CCE处理组和对照组细胞的β-半乳糖苷酶活性和RoS水平的变化.Hela细胞经CCE处理72h之后,提取RNA,通过实时荧光定量实验检测CAT、SoD1、SoD2、GPX1等抗氧化基因的表达量.结果表明:CCE抑制Hela细胞的增殖,且呈剂量依赖效应,较低浓度的CCE(≦0.100mg/ml)对细胞生长无明显抑制作用.0.100mg/ml的CCE处理Hela细胞72h后能够显著地促进CAT和SoD1等基因的转录,从而降低H_2o_2产生的过量RoS,抑制细胞衰老.

关键词：蝉花活性氧抗氧化抗衰老

Scapula Fracture in a Child: Report of a Rare Injury

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open Journal of Pediatrics 2023年第2期13卷 158-163页

作者： Florent Tshibwid A. Zeng Mbaye Fall Nankouman Konaté Faty Balla lô Eben-Ezer Samé Souleymane Camara Aimé lakh Faye Fall oumar Ndour Gabriel Ngom Department of Pediatric Surgery Aristide Le Dantec University Teaching Hospital Cheikh Anta Diop University Dakar Senegal Department of Pediatric Surgery Albert Royer National Children’s Hospital Center Cheikh Anta Diop University Dakar Sene-gal

Scapular fracture is exceptional in children, mainly occurring after high-energy trauma. Radiologic investigations help its diagnosis and classification, which determines its management. We report the case of a 14-year-old patient admitted for blunt trauma of the left shoulder after falling from a speeding car. The diagnosis of a displaced fracture of the body of the scapula was made, and non-operative treatment was indicated and carried out for four weeks, followed by physiotherapy for another four weeks. Nine weeks after the trauma, the mobility of the affected shoulder was equivalent to that of the contralateral shoulder.

关键词： Fracture Scapula Child Non-operative Management Case Report

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震后一分钟:强震图、有效震中和有效震级

世界地震译丛 1998年第5期29卷 50-59页

作者： T.l.Deng l.D.Wu T.C.Shin Y.B.Tsai W.H.K.lee 马维四川省地震局

本文报道了台湾实时地震监测的最新进展,尤其是台湾中心气象局遥测地震台网(CWBSN)的实时强震监测。该项工作旨在大震发生后迅即速报。如果速报能在强振动到来之前完成,那么地震早期警报将成为可能。CWBSN已能在大震发生后一分钟内给出... 详细信息

本文报道了台湾实时地震监测的最新进展,尤其是台湾中心气象局遥测地震台网(CWBSN)的实时强震监测。该项工作旨在大震发生后迅即速报。如果速报能在强振动到来之前完成,那么地震早期警报将成为可能。CWBSN已能在大震发生后一分钟内给出强度图、震中和震级。速报和早期警报主要适用于大震(M>>5);不限幅波形记录的需要促使CWBSN在1995年将强震探测仪(如力平衡加速度计)并入其遥测地震监测系统中。将时间域递归处理应用于多道输入地震信号,通过一组联网微机生成强度图。通过等值线,能迅速识别出位于强度图最大等值线(通常为100gal)中央的有效震中。有效震级也能迅速由最大等值线(通常为100gal)覆盖的表面积确定。对于具有有限破裂面的大震,如此得出的震中和震级是更适当的震源位置和破坏强度的估算值。有效震中给出了破坏区域的中心,与传统的震中定位比较,后者仅给出了破裂形成的初始位置。有效震级所反映的更接近地震破坏的潜能,而不是强调总能量释放的传统震级。CWBSN已能在一分钟内获取上述震源的关键信息。这一时间将会进一步缩短至30s以内,如本文中举例描述的那样,地震早期警报的确是一个可能达到的目标。速报和早期警报信息将电传给用户,以采取人为或无人干予的快速反应行动。

关键词：地震强震图有效震中有效震级

Transport and Deposition of Saharan Dust observed from Satellite Images and Ground Measurements

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Journal of Atmospheric Science Research 2021年第2期4卷 1-11页

作者： Habib Senghor Alex J.Roberts Abdou l.Dieng Dahirou Wane Cheikh Dione Mouhamed Fall Abdoulahat Diop Amadou T.Gaye John Marsham Agence nationale de l’aviation civile et de la météorologie Sénégal laboratoire de Physique de l’Atmosphère et de l’océan Simeon-Fongang(lPAo-SF) École Supérieure Polytechnique(ESP)de l’UniversitéCheikh Anta Diop(UCAD)DakarSénégal School of Earth and Environment University of LeedsLS29JTUK African Centre of Meteorological Applications for Development(ACMAD) Niger

Haboob occurrence strongly impacts the annual variability of airborne desert dust in North *** fact,more dust is raised from erodible surfaces in the early summer(monsoon)season when deep convective storms are common but soil moisture and vegetation cover are *** 27 June 2018,a large dust storm is initiated over North Africa associated with an intensive westward dust *** away from emission sources,dust is transported over the Atlantic for the long *** plume is emitted by a strong surface wind and further becomes a type of haboob when it merges with the southwestward deep convective system in central Mali at 0200 UTC(27 June).We use satellite observations to describe and estimate the dust mass concentration during the *** 93%of emitted dust is removed the dry deposition from the atmosphere between sources(10°N–25°N;1°W–8°E)and the African coast(6°N–21°N;16°W–10°W).The convective cold pool has induced large economic and healthy damages,and death of animals in the northeastern side of ***5 reanalysis has shown that the convective mesoscale impacts strongly the climatological location of the Saharan heat low(SHl).

关键词： Dust Haboob Saharan air layer

利用拮抗、ITS和RAPD技术对灵芝属菌株分类的研究

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西南农业学报 2017年第1期30卷 26-33页

作者：王锦锋李晶 I.l.Datti 张健林志魁林占熺福建农林大学园艺学院福建福州350002 国家菌草工程技术研究中心福建福州350002 福建农林大学生命科学学院福建福州350002

为了更科学的对灵芝菌株进行分类与鉴定,利用拮抗、ITS和RAPD技术对21株灵芝菌株进行分类鉴定和遗传多样性分析。拮抗试验结果表明,大部分菌株之间存在不同程度的拮抗作用,少数菌株之间没有拮抗或拮抗极不明显;ITS序列分析结果表明,21... 详细信息

为了更科学的对灵芝菌株进行分类与鉴定,利用拮抗、ITS和RAPD技术对21株灵芝菌株进行分类鉴定和遗传多样性分析。拮抗试验结果表明,大部分菌株之间存在不同程度的拮抗作用,少数菌株之间没有拮抗或拮抗极不明显;ITS序列分析结果表明,21个菌株分为3个组,菌株间遗传距离的变化范围在0.000~0.072,平均遗传距离为0.039,菌株1号(赤68)与3号(Ga15)的遗传距离为0.000,证明其亲缘性较近;RAPD结果表明,各菌株间的相似系数在0.83以上,所有菌株分为两个组,4号(867)菌株单独为1组,其它20个菌株为1组;相似系数在0.932的相似水平上,所有菌株分为14个组;1号菌株与3号菌株的相似系数则超过了0.95,有可能是同种异名。3种方法其结论一致,且能准确鉴定21株灵芝亲缘关系。

关键词：拮抗试验 ITS序列分析 RAPD技术遗传多样性亲缘性

Study of the production of Λ_b^0 band ~0 hadrons in pp collisions and first measurement of the Λ_b^0→J/ψpK^- branching fraction

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Chinese Physics C 2016年第1期40卷 1-16页

作者： o.Kochebina M.Kolpin I.Komarov R.F.Koopman P.Koppenburg M.Kozeiha l.Kravchuk K.Kreplin M.Kreps G.Krocker P.Krokovny F.Kruse W.Krzemien W.Kucewicz M.Kucharczyk V.Kudryavtsev A.K.Kuonen K.Kurek T.Kvaratskheliya D.lacarrere G.lafferty A.lai D.lambert G.lanffanchi C.langenbruch B.langhans T.latham C.lazzeroni R.le Gac J.van leerdam J.-P.lees R.lefevre A.leflat J.lefrancois E.lemos Cid o.leroy T.lesiak B.leverington Y.li T.likhomanenko M.liles R.lindner C.linn F.lionetto B.liu X.liu D.loh I.longstaff J.H.lopes D.lucchesi M.lucio Martinez H.luo A.lupato E.luppi o.lupton A.lusiani F.Machefert F.Maciuc o.Maev K.Maguire S.Malde A.Malinin G.Manca G.Mancinelli P.Manning A.Mapelli J.Maratas J.F.Marchand U.Marconi C.Marin Benito P.Marino J.Marks G.Martellottil M.Martin M.Martinelli D.Martinez Santos F.Martinez Vidal D.Martins Tostes A.Massafferri R.Matev A.Mathad Z.Mathe C.Matteuzzi A.Mauri B.Maurin A.Mazurov M.McCann J.McCarthy A.McNab R.McNulty B.Meadows F.Meier M.Meissner D.Melnychuk M.Merk E Michielin D.A.Milanes M.-N.Minard D.S.Mitzel J.Molina Rodrigue I.A.Monroy S.Monteil M.Morandin P.Morawski A.Morda M.J.Morello J.Moron A.B.Morris R.Mountain F.Muheim D.Miiller J.Muller K.Muller V.Muller M.Mussini B.Muster P.Naik T.Nakada R.Nandakumar A.Nandi I.Nasteva M.Needham N.Neri S.Neubert N.Neufeld M.Neuner A.D.Nguyen T.D.Nguyen C.Nguyen-Mau V.Niess R.Niet N.Nikitin T.Nikodem D.Ninci A.Novoselov D.P.o'Hanlon A.oblakowska-Mucha V.obraztsov S.ogilvy o.okhrimenko R.oldeman C.J.G.onderwater B.osorio Rodrigues J.M.otalora Goicochea A.otto P.owen A.oyanguren A.Palano F.Palombo M.Palutan J.Panman A.Papanestis M.Pappagallo l.l.Pappalardo C.Pappenheimer C.Parkes G.Passaleva G.D.Patel M.Patel C.Patrignani A.Pearce A.Pellegrino G.Penso M.Pepe Altarelli S.Perazzini P.Perret l.Pescatore K.Petridis A.Petrolini M.Petruzzo E.Picatoste olloqui B.Pietrzyk T:.Pilar D.Pinci A.Pistone A.Piucci S.Playfer M.Plo Casasus T.Poikela F.Polci A.Poluektov I.Polyakov E.Polycarpo A.Popov D.Popov B.Popovici C.Potterat E.Price J.D.Price J.Prisciandaro A.Pritchard Centro Brasileiro de Pesquisas Ffsicas(CBPF) Rio de JaneiroBrazil Universidade Federal do Rio de Janeiro(UFRJ) Rio de JaneiroBrazil Center for High Energy Physics Tsinghua UniversityBeijingChina lAPP Universite Savoie Mont-BlancCNRS/IN2P3Annecy-Le-VieuxFrance Clermont Universite Universite Blaise PascalCNRS/IN2P3LPCclermont-FerrandFrance CPPM Aix-Marseille UniversitYCNRS/IN2P3MarseilleFrance lAl Universite Paris-SudCNRS/IN2P3OrsayFrance lPNHE Universite Pierre et Marie CurieUniversite Paris DiderotCNRS/IN2P3ParisFrance Fakultat Physik Technische Universitat DortmundDortmundGermany Max-Planck-lnstitut fur Kernphysik(MPIK) HeidelbergGermany Physikalisches Institut Ruprecht-Karls-Universitat HeidelbergHeidelbergGermany School of Physics University College DublinDublinIreland Sezione INFN di Bari BariItaly Sezione INFN di Bologna BolognaItaly Sezione INFN di Cagliari CagliariItaly Sezione INFN di Ferrara FerraraItaly Sezione INFN di Firenze FirenzeItaly laboratori Nazionali dell'INFN di Prascati FrascatiItaly Sezione INFN di Genova GenovaItaly Sezione INFN di Milnno Bicocca MilunoItaly Sezione INFN di Milano MilanoItaly Sezione INFN di Padova PadovaItaly Sezione INFN di Pisa PisaItaly Sezione INFN di Roma Tor Vergata RomaItaly Sezione INFN di Roma la Sapienza RomaItaly Henryk Niewodniczanski Institute of Nuclear Physics Polish Academy of Sciences Krak6wPoland AGH-University of Science and Technology Faculty of Physics and Applied Computer ScienceKrakowPoland National Center for Nuclear Research(NCBJ) WarsawPoland Horia Hulubei National Institute of Physics and Nuclear Engineering Bucharest-MagureleRomania Petersburg Nuclear Physics Institute(PNPI) GatchinaRussia Institute of Theoretical and Experimental Physics(ITEP) MoscowRussia Institute of Nuclear Physics Moscow State University(SINP MSU)MoscowRussia Institute for Nuclear Research of the Russian Academy of Sciences(INR RAN) MoscowRussia Budker Institute of Nuclear Physics(SB RAS)and Novosibirsk State University No

The product of the ∧0/b （-B/0） differential production cross-section and the branching fraction of the decay ∧0/b→ J/ψ pK-（-B/0→ J/ψ-K＊（892）0）is measured as a function of the beauty hadron transverse momentum, PT, and rapidity, y. The kinematic region of the measurements is pT〈20 GeV/c and 2.0 〈g〈*** measurements use a data sample corresponding to an integrated luminosity of 3fb-1 collected by the lHCb detector in pp collisions at centre-of-mass energies √s=7 TeV in 2011 and √s=8 TeV in 2012. Based on previous lHCb results of the fragmentation fraction ratio,f∧0/b/fd,the branching fraction of the decay ∧0/b→ J/ψ pK-is measured to be B（∧0/b→ J/ψ pK-）=（3.17±0.04±0.07±0.34＋0.45/-0.28）×10-4,where the first uncertainty is statistical, the second is systematic, the third is due to the uncertainty on the branching fraction of the decay -B/0 →J/ψ-K＊（892）0,and the fourth is due to the knowledge of f∧0/b/*** sum of the asymmetries in the production and decay between ∧0/b and ∧0/bis also measured as a function of PT and *** previously published branching fraction of ∧0/b→ J/ψ pπ-,relative to that of ∧0/b→ J/ψ pK-,is updated. The branching fractions of ∧0/b→P＋c（→ J/ψp）K-are determined.

关键词： production cross-section branching fraction b hadrons proton-proton collisions

Inoculation with arbuscular mycorrhizal fungi improves seedlings growth of two sahelian date palm cultivars (<i>Phoenix dactylifera</i>l., cv. Nakhla hamra and cv. Tijib) under salinity stresses

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Advances in Bioscience and Biotechnology 2014年第1期5卷 64-72页

作者： Ibrahima léopold Djitiningo Diatta Aboubacry Kane Codjo Emile Agbangba Maurice Sagna Diegane Diouf Frédérique Aberlenc-Bertossi Yves Duval Alain Borgel Djibril Sane IRD Institut de Recherche pour le Développement Montpellier France laboratoire Campus de Biotechnologies Végétales Département de Biologie Végétale Faculté des Sciences et Techniques Un-iversité Cheikh Anta Diop Dakar Sénégal laboratoire Campus de Biotechnologies Végétales Département de Biologie Végétale Faculté des Sciences et Techniques Un-iversité Cheikh Anta Diop Dakar Sénégal laboratoire Commun de Microbiologie IRD/ISRA/UCAD Route des Hydrocarbures Dakar Sénégal laboratoire d’Ecologie et d’Ecohydrologie Département de Bi-ologie Végétale Faculté des Sciences et Techniques Université Cheikh Anta Diop Dakar Sénégal

This study presents an analysis of the impact of mycorrhizal inoculation on growth under salt stress of date palms cultivars Nakhla hamra (NHH) and Tijib known in the Sahel for their earliness in flowering and fruiting. The seedlings were grown in a greenhouse on a sandy substrate watered to field capacity every two days and were subjected to increasing levels of NaCl (0, 1, 2, 4, 6, 8 and 16 g·l-1) and then inoculated with 5 strains of Glomus (G. aggregatum, G. intraradices, G. verriculosum, G. mosseae, G. fasciculatum). The experimental design was a randomized complete block with three factors (cultivars: Tijib and Nakhla Hamra x seven levels of NaCl concentrations x six levels of inoculum). The results showed that in the absence of NaCl, G. verriculosum significantly enhanced shoot growth: 33.5 cm against 30.3 cm in the control and roots growth: 81.5 cm against 78 cm in NHH, while in Tijib, the growth is stimulated by Glomus fasciculatum: 33.8 cm against 32.7 cm for stems and 90 cm against 86 cm for the roots of inoculated plants and controls. In contrast, in the presence of NaCl 8 g·l-1, NHH has a better growth in the presence of G. intraradices: 37.3 cm against 30.6 cm for stems and 77 cm against 73 for roots, while Tijib grows better in the presence of G. fasciculatum with respectively 31.9 cm against 31.7 cm and 51.27 cm against 51.6 cm for stems and roots of inoculated plants and controls. Biochemical analysis revealed that changes in levels of proline depend on the cultivar, the mycorrhizal strain used and concentrations of NaCl applied. These results open the prospect of using mycorrhizal fungi to improve the productivity of palm trees in the Sahel.

关键词： Sahel Date Palm Salinity Inoculation Mycorrhizae Glomus Proline

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卵巢癌唾液酸转移酶mRNA表达的变化

世界核心医学期刊文摘（妇产科学分册） 2006年第5期2卷 34-35页

作者： lee W.-l Juang C.-M. 朱亮

Aberrant glycosylation occurs in essentially all types of experimental and human cancers, and many glycosyl epitopes constitute tumor-associated antigens (for example, CA125). Many recent studies have indicated that some, if not all, aberrant glycosylation is a result of altered sialyltransferase (ST) expression; however, there is little known of the role of the altered mRNA expression of ST in ovarian cancers. Methods. Alterations in ST mRNA expression in postmenopausal ovarian tissues, including those of normal controls (n = 24) and malignant serous ovarian cancers (n = 24), were examined by means of real-time quantitative reverse transcription-polymerase chain reaction (RTQ-PCR). Maackia Amurensis Agglutin in type 2 (MAA) specific for α 2,3- linked NeuNAc was used for immunohistochemical staining. Results. Among these five STs, the mRNA expressions of three STs, including ST3gal III, ST3gal IV, and ST3gal VI, were significantly decreased in patients with ovarian cancers, compared to the normal controls (P ontrast, the mRNA expressions of ST3gal I and ST6gal I were increased in ovarian cancer tissues, compared to those of the normal controls (P ovarian epithelial carcinoma part showed strong positivity for MAA, whereas MAA staining in the stromal part was negative. Both the epithelial part and the stromal part of postmenopausal ovarian tissue showed negativity for MAA staining. However, clinico-pathological parameters, including stage, differentiation, amount of ascites, and serum levels of CA125, did not show any correlation to mRNA expression of any given-type ST. Conclusions. our results suggest that altered mRNA expressions of α 2,3- sialyltransferase ST3gal I, ST3gal III, ST3gal IV, ST3gal VI, and α 2,6- sialyltransferase ST6gal I are of importance in malignant ovarian cancers. An increased expression of ST3gal I may contribute directly to increased α 2,3- linked sialylation in ovarian serous carcinoma.

关键词：唾液酸转移酶卵巢组织肿瘤相关抗原唾液酸化作用病理学参数免疫组化染色间质部抗原决定簇山

Identification of the S-layer glycoproteins and their covalently linked glycans in the halophilic archaeon Haloarcula hispanica

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Identification of the S-layer glycoproteins and their covale...