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Callogenesis and Antibacterial Activity of Balanites aegyptiaca

Journal of Biosciences and Medicines 2020年第10期8卷 157-168页

作者： Gali Adamu Ishaku Aishatu Haruna Ayuba Abaka Kalum Celia Vargas-De-La-Cruz richard solorzano-acosta Department of Biotechnology School of Life SciencesModibbo Adama University of TechnologyYolaAdamawa StateNigeria Department of Crop Protection School of Agriculture and Agricultural TechnologyModibbo Adama University of TechnologyYolaAdamawa StateNigeria Facultad de Farmacia y Bioquimica Departamento Academico de FarmacologíaBromatología y ToxicologiaCentro Latinoamericano de Enseñanza e Investigación en Bacteriologia Alimentaria(CLEIBA)Universidad Nacional Mayor de San MarcosLimaPeru Research Group Biotechnology and Omics in Life Sciences Universidad Nacional Mayor de San MarcosLimaPeru Facultad de Farmacia y Bioquímica Universidad Norbert WienerLimaPeru

B. aegyptiaca, it is a species of economic and cultural importance in various countries, with diverse uses that include: medicinal, charcoal, pesticides and forage and in vitro callus production is important to have many applications in both basic and industrial research on this specie. For the induction of callus, B. aegyptica seed cotyledons were surface sterilized with 90% ethanol for 1 minute and cultivated in MS media supplemented with 2,4-D, BAP and NAA. Both the callus and seed were collected and dried in an oven at 40°C - 45°C. Cotyledon’s seed and callus were grounded into the powdered form using mortar and pestle and stored at room temperature for further use. Five grams (5 g) each of the powder were mixed with 50 ml of the solvents: methanol and n-hexane (1:10) w/v, agitated vigorously and kept on an orbital shaker at 150 rpm for 24 h, then filtered. The extracts of the plant sample were evaluated in agar dilution method which was used to determine the MIC and MBC of the extracts. The auxin NAA in low concentrations (0.5 mg/L) in the presence of a dose of 0.5 mg/L of the cytokinin BAP induced 100% callus formation. The 50 and 100 mg/ml methanolic extracts were more effective than the n-hexane extracts for both the gram-positive and gram-negative bacteria. By callus extracts under 100 and 50 mg/ml reveals that methanolic extracts of callus had the highest zone of inhibition. An effective protocol for callus induction has been developed that can use for germplasm conservation or for genetic engineering. Evidence from the present study revealed both extracts possess strong broad-spectrum antibacterial effect. Therefore, methanolic extract of seed kernel callus of B. aegyp

关键词： Antibacterial Activities Callus Extract Callus Induction Minimum Inhibitory Concentration and Seed Cotyledon Extract

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Surgical management of peritonitis secondary to acute superior mesenteric artery occlusion

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World Journal of Gastroenterology 2014年第29期20卷 9936-9941页

作者： Stefan acosta Vascular Centre Malm?Sk?ne University Hospital

Diagnosis of acute arterial mesenteric ischemia in the early stages is now possible using modern computed tomography with intravenous contrast enhancement and imaging in the arterial and/or portal *** patients have acute superior mesenteric artery(SMA)occlusion,and a large proportion of these patients will develop peritonitis prior to mesenteric revascularization,and explorative laparotomy will therefore be necessary to evaluate the extent and severity of intestinal ischemia,and to perform bowel *** establishment of a hybrid operating room in vascular units in hospitals is most important to be able to perform successful intestinal *** review outlines current frontline surgical strategies to improve survival and minimize bowel morbidity in patients with peritonitis secondary to acute SMA *** laparotomy needs to be performed *** treatment is based upon intestinal revascularization followed by bowel *** no vascular imaging has been carried out,SMA angiography is *** case of embolic occlusion of the SMA,open embolectomy is performed followed by *** case of thrombotic occlusion,the occlusive lesion can be recanalized retrograde from an exposed SMA,the guidewire snared from either the femoral or brachial artery,and stented with standard devices from these access *** resections and sometimes gall bladder removal due to transmural infarctions are performed at initial laparotomy,leaving definitive bowel reconstructions to a planned second look laparotomy,according to the principles of damage control *** with peritonitis secondary to acute SMA occlusion should be managed by both the general and vascular surgeon,and a hybrid revascularization approach is of utmost importance to improve outcomes.

关键词： Acute mesenteric ischemia Peritonitis Explorative laparotomy Endovascular treatment Hybrid revascularization Superior mesenteric artery occlusion

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New unexpected role for Wolfram Syndrome protein WFS1:a novel therapeutic target for Alzheimer’s disease?

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Neural Regeneration Research 2023年第7期18卷 1501-1502页

作者： Shuo Chen Diana acosta Hongjun Fu Department of Neuroscience Biomedical Sciences Graduate ProgramThe Ohio State UniversityColumbusOHUSA Department of Neuroscience The Ohio State UniversityColumbusOHUSA

Selective vulnerability of excitatory neurons in Alzheimer’s disease(AD):AD is the most common form of dementia;however,the pathogenesis of AD is largely *** of the characteristic features of AD is the formation of intracellular neurofibrillary tangles(NFTs).NFTs are abnormal accumulates of misfolded tau protein,which may eventually cause neuronal death and neurodegeneration(Jack et al.,2018).In the early stages of AD progression,not all neurons are equally vulnerable to tau *** studies have shown that large pyramidal neurons in the entorhinal cortex(EC)are specifically vulnerable to pathological tau accumulation(Fu et al.,2017).This selective vulnerability of excitatory neurons to tau pathology is one of the fundamental questions needed to be answered in AD research.

关键词： Alzheimer tau cortex

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Stability results of properties related to the Bishop-Phelps-Bollobás property for operators

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Science China Mathematics 2021年第5期64卷 1011-1028页

作者： Maria D.acosta Maryam Soleimani-Mourchehkhorti Facultad de Ciencias Departamento de Analisis MatematicoUniversidad de GranadaGranada 18071Spain School of Mathematics Institute for Research in Fundamental Sciences(IPM)Tehran 19395-5746Iran

We prove that the class of Banach spaces Y such that the pair(l_(1),Y)has the Bishop-Phelps-Bollobás property for operators is stable under finite products when the norm of the product is given by an absolute *** als... 详细信息

关键词： Banach space operator Bishop-Phelps-Bollobás theorem Bishop-Phelps-Bollobás property

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A PD-Type State-Dependent Riccati Equation With Iterative Learning Augmentation for Mechanical Systems

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IEEE/CAA Journal of Automatica Sinica 2022年第8期9卷 1499-1511页

作者： Saeed Rafee Nekoo JoséÁngel acosta Guillermo Heredia Anibal Ollero GRVC Robotics Lab. Depto de Ingeniería de Sistemas y AutomáticaEscuela Técnica Superior de IngenieríaUniversidad de SevillaSeville 41092Spain

This work proposes a novel proportional-derivative(PD)-type state-dependent Riccati equation(SDRE)approach with iterative learning control(ILC)*** the one hand,the PD-type control gains could adopt many useful available criteria and tools of conventional PD *** the other hand,the SDRE adds nonlinear and optimality characteristics to the controller,i.e.,increasing the stability *** advantages with the ILC correction part deliver a precise control law with the capability of error reduction by *** SDRE provides a symmetric-positive-definite distributed nonlinear suboptimal gain K(x)for the control input law u=–R–1(x)BT(x)K(x)*** sub-blocks of the overall gain R–1(x)BT(x)K(x),are not necessarily symmetric positive definite.A new design is proposed to transform the optimal gain into two symmetric-positive-definite gains like PD-type controllers as u=–KSP(x)e–KSD(x)?.The new form allows us to analytically prove the stability of the proposed learning-based controller for mechanical systems;and presents guaranteed uniform boundedness in finite-time between learning *** symmetric PD-type controller is also developed for the state-dependent differential Riccati equation(SDDRE)to manipulate the final *** SDDRE expresses a differential equation with a final boundary condition,which imposes a constraint on time that could be used for finitetime ***,the availability of PD-type finite-time control is an asset for enhancing the conventional classical linear controllers with this *** learning rules benefit from the gradient descent method for both regulation and tracking *** of the advantages of this approach is a guaranteed-stability even from the first loop of learning.A mechanical manipulator,as an illustrative example,was simulated for both regulation and tracking *** experimental validation was done to show the capability of the system in practice by the implementation of the proposed metho

关键词： Closed-loop iterative learning control(ILC) PD-type SDRE SDDRE symmetric

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Design and Construction of a Pneumatic Temporary Immersion Bioreactor System for the Multiplication of <i>Ananas comosus</i>var. Trujillana Red

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American Journal of Plant Sciences 2020年第9期11卷 1429-1442页

作者： richard Solórzano-acosta Marlene Guerrero-Padilla Departamento de Fitotecnia Facultad de Agronomía Universidad Nacional Agraria La Molina (UNALM) Lima Perú Facultad de Ciencias Biológicas Universidad Nacional de Trujillo Perú

The present work initially identified the design parameters of a temporary immersion bioreactor to later scale it to a complete system for the in vitro multiplication of Ananas comosus var. Trujillana Red. Thus, a low-cost pneumatic temporary immersion bioreactor system was designed and built with 24 tanks of 2 L each. The automation of the system was designed and implemented by means of a timer circuit whose design parameters were: duration of the propagation process, which depends on the multiplication period of the crop and is an open variable, which means that the operator decides when to turn off the system;the duration of each dive, which for reasons of complexity of the algorithm was standardized as one minute;immersion frequency, which was programmed for intervals of 1, 2, 3, 4, 5, 6, 7, 8 hours respectively and duration of aeration, which from a test run times of 0.20 were chosen, 30, 40, 50, 60, 70, and 80 seconds that correspond to the time of delivery of compressed air;additionally, the multiplication rate of Ananas comosus var. Trujillana Red in the immersion system which was 6.5 times per propagative unit inoculated in thirty days.

关键词： Temporary Immersion Ananas comosus Bioreactors Micropropagation

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Actin levels fluctuate in the seminiferous epithelium at various stages during spermatogenesis in the rat

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BIOCELL 2017年第2期41卷 63-65页

作者： CRISTIAN acosta MABEL FOSCOLO JUAN CARLOS CAVICCHIA Instituto de Histología y Embriología de Mendoza(IHEM)-CONICET Universidad Nacional de Cuyo5500MendozaArgentina

The testis is a double gland comprised of the sperm-producing seminiferous tubules and a complex endocrine *** former structures generate and release whole cells(exocrine aspect of the gland)while the latter synthesize and release androgens and related *** testis also has poorly understood paracrine *** connexions play an important role during spermatogenesis.A key molecule within these structures is β-***,the present study aims at examining the expression pattern of β-actin during the various stages of the spermatic cycle in the *** achieve this goal,we used a combination of trans-illumination assisted microdissection of seminiferous tubules,confocal immunofluorescence and Western blot *** experiments revealed that the levels of β-actin fluctuate significantly during spermatogenesis,peaking immediately after spermiation.

关键词： Beta actin Seminiferous epithelium Trans-illumination assisted microdissection

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Testing the hypothesis of subtypes of nonadherence in schizophrenia and schizoaffective disorder:A prospective study

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World Journal of Psychiatry 2020年第11期10卷 260-271页

作者： Dulcinea Vega Francisco J acosta Pedro Saavedra Department of Psychiatry University of Las Palmas de Gran CanariaLas Palmas de Gran Canaria 35016Las PalmasThe Canary IslandsSpain Department of Psychiatry Insular University Hospital Department of Mental Health General Management of Healthcare Programs Canary Islands Health ServiceLas Palmas de Gran Canaria 35004Las PalmasThe Canary IslandsSpain Research Network on Health Services for Chronic Conditions(REDISSEC) Carlos III Health InstituteMadrid 28029Spain Department of Mathematics University of Las Palmas de Gran CanariaLas Palmas de Gran Canaria 35016Las PalmasThe Canary IslandsSpain

BACKGROUND Nonadherence is a major problem in the treatment of psychotic *** has been hypothesized that nonadherent patients with schizophrenia are not a homogeneous population and subtypes of nonadherence might exist,but this hypothesis has not been specifically *** To test the hypothesis of subtypes of nonadherence in schizophrenia and schizoaffective *** This prospective study included 110 consecutively admitted patients diagnosed with schizophrenia or schizoaffective *** were performed at baseline and at 6 mo follow-up after ***,clinical,psychopathological and treatment-related variables were *** was defined as the concurrence of adherence to antipsychotic treatment and outpatient follow-up during the six-month *** to antipsychotic treatment was defined as the concurrence of objective and subjective ***-four patients(58%)fulfilled nonadherence criteria at the end of the followup period and were categorized according to their subtype of *** In nonadherent patients(n=64),32(50%)fulfilled criteria of intentional nonadherence,and 32(50%)of unintentional nonadherence(UNA).Unintentional nonadherent patients,as compared to intentional nonadherent patients,are characterized by older age,lower educational level,worse cognitive and negative symptoms,greater severity,worse knowledge of their treatment regimen,greater prevalence of supervision of the treatment,lower number of prior hospitalizations and greater use of nonpsychiatric treatment,anticholinergics and *** educational level(OR=26.1;95%CI:2.819-241),worse treatment knowledge at six months(OR per unit=0.904;95%CI:0.853-0.957)and nonpsychiatric treatment at six months(OR=15.8;95%CI:1.790-139)were independently associated to *** Differentiated subtypes of nonadherence according to intentionality seem to exist in patients with schizophrenia and schizoaffective ***

关键词： Adherence Hospitalization Psychosis Hypothesis Unintentional nonadherence Intentional nonadherence

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Monitoring of the Corrosion on a Steel Sheet-Pile Marine Breakwater by Systematic Thickness Measurements

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Journal of Civil Engineering and Architecture 2018年第1期12卷 39-50页

作者： Luis Osmel Millan solorzano Department Millan Engineering San Jose 30303 Costa Rica

In Quepos, Pacific of Costa Rica, it was finished on 2010 the first phase of a marina, including two mix breakwaters, with rubble mound （rocks and concrete units）, and 25 circular steel sheet piles cofferdam cells, filled with sand and gravel. The maintenance plan, considers tracking sheet pile corrosion, comparing ＂actual＂ against expected rates, checking structural limits, and programming countermeasures if accelerated corrosion is identified. Specific control sections, along the breakwaters, both inside and outside the basin, were established. In each section, thicknesses were measured every meter from the top of the steel cell to seabed using an ultrasonic equipment, and an underwater transducer. Both land crew, and divers for submerged portions, were used. The measurements campaigns are for several years from 2011 to 2016. Sectors of the breakwater with varied corrosion attack levels could be differentiated. Also, corrosion rates and lifespans were estimated, both general for the structures, and specific for each section and level. In turn, this allowed to identify maintenance priorities, defining sites where measures of corrosion protection should initiate, as well, to have confidence in the structural capacity and safety of the breakwaters.

关键词： Monitoring of structures maritime works sheet piling corrosion ultrasonic thickness measurements.

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A Variational Approach to Norm Attainment of Some Operators and Polynomials

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Acta Mathematica Sinica,English Series 2010年第12期26卷 2259-2268页

作者： María D.acosta Jernimo ALAMINOS Domingo GARCA Manuel MAESTRE Departamento de Anlisis Matemtico Universidad de Granada Departamento de Anlisis Matemtico Universidad de Valencia

If X is an Asplund space, then every uniformly continuous function on Bx＊ which is holomorphic on the open unit ball, can be perturbed by a w＊ continuous and homogeneous polynomial on X＊ to obtain a norm attaining function on the dual unit ball. This is a consequence of a version of Bourgain-Stegall＇s variational principle. We also show that the set of N-homogeneous polynomials between two Banach spaces X and Y whose transposes attain their norms is dense in the corresponding space of N-homogeneous polynomials. In the case when Y is the space of Radon measures on a compact K, this result can be strengthened.

关键词： Banach space variational principle operator polynomial holomorphic function

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