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Epididym.s-specific lipocalin prom.ters

Asian Journal of Andrology 2007年第4期9卷 515-521页

作者： Kichiya Suzuki Xiuping Yu Pierre Chaurand Yoshihiko Araki Jean-Jacques Lareyre richard m. caprioli m.rie-Claire Orgebin-Crist Robert J. m.tusik Departm.nt of Obstetrics and Gynecology Tohoku University Graduate School of Medicine Sendai 980-8574 Japan Departm.nts of Urologic Surgery Vanderbilt University School of Medicine Nashville Tennessee 37232 USA Departm.nts of Biochem.stry Vanderbilt University School of Medicine Nashville Tennessee 37232 USA Departm.nts of Obstetrics and Gynecology Vanderbilt University School of Medicine Nashville Tennessee 37232 USA Departm.nts of Cell and Developm.ntal Biology Vanderbilt University School of Medicine Nashville Tennessee 37232 USA Departm.nts of m.ss Spectrom.try Research Center and Vanderbilt University School of Medicine Nashville Tennessee 37232 USA Departm.nts of Center for Reproductive Biology Research Vanderbilt University School of Medicine Nashville Tennessee 37232 USA

Our goal is to decipher which DNA sequences are required for tissue-specific expression of epididym.l genes. At least 6 epididym.s-specific lipocalin genes are known. These are differently regulated and regionalized in the epididym.s. Lipocalin 5 （Lcn5 or m.-RABP） and Lipocalin 8 （Lcn8 or m.P17） are hom.logous genes belonging to the epididym.s-specific lipocalin gene cluster. Both the 5 kb prom.ter fragm.nt of the Lcn5 gene and the 5.3 kb prom.ter fragm.nt of the Lcn8 gene can direct transgene expression in the epididym.s （Lcn5 to the distal caput and Lcn8 to the initial segm.nt）, indicating that these prom.ter fragm.nts contain im.ortant cis-regulatory elem.nt（s） for epididym.sspecific gene expression. To define further the fragm.nts regulating gene expression, the Lcn5 prom.ter was exam.ned in transgenic m.ce and im.ortalized epididym.l cell lines. After serial deletion, the 1.8 kb prom.ter fragm.nt of the Lcn5 gene was sufficient for tissue-specific and region-specific gene expression in transgenic m.ce. Transient transfection analysis revealed that a transcription factor forkhead box A2 （Foxa2） interacts with androgen receptor and binds to the 100 bp fragm.nt of the Lcn5 prom.ter between 1.2 kb and 1.3 kb and that Foxa2 expression inhibits androgen-dependent induction of the Lcn5 prom.ter activity. Im.unohistochem.stry indicated a restricted expression of Foxa2 in the epididym.s where endogenous Lcn5 gene expression is suppressed and that the Foxa2 inhibition of the Lcn5 prom.ter is consistent with the lack of expression of Lcn5 in the corpus and cauda. Our approach provides a basic strategy for further analysis of the epididym.l lipocalin gene regulation and flexible control of epididym.l function. （Asian J Androl 2007 July; 9： 515-521）

关键词： androgen epididym.s gene cluster gene evolution gene regulation lipocalin transcription factor

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In situ m.ss spectrom.try of autoim.une liver diseases

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Cellular & m.lecular Im.unology 2011年第3期8卷 237-242页

作者： Christopher L Bowlus Erin H Seeley Joanna Roder Julia Grigorieva Heinrich Roder richard m.caprioli m.Eric Gershwin Division of Gastroenterology and Hepatology University of CaliforniaDavisCAUSA m.ss Spectrom.try Research Center Vanderbilt University School of MedicineNashvilleTNUSA Biodesix Steamboat SpringsCOUSA Division of Clinical Im.unology Allergy and RheumatologyUniversity of CaliforniaDavisCAUSA

Prim.ry biliary cirrhosis(PBC),prim.ry sclerosing cholangitis(PSC)and autoim.une hepatitis(AIH)are the m.jor form. of autoim.une liver diseases each characterized by the destruction of a specific liver cell type and the presence of differing *** took a proteom.c approach utilizing in situ m.trix-assisted laser desorption/ionization m.ss spectrom.try(m.LDI m.)to obtain profiles directly from.liver sam.les of patients with PBC,PSC,AIH and *** ability to precisely localize the region for acquisition of m.LDI m. allowed us to obtain profiles from.bile ducts,inflam.atory infiltrates and hepatocytes from.each biopsy *** tools developed to identify peaks and com.are peaks across diseases and cell types were used to develop m.dels to classify the *** an initial set of testing sam.les from.PBC patients and controls,we identified unique peaks present in bile ducts,inflam.atory infiltrates and hepatocytes that could classify sam.les in a validation cohort with 88–91%***,profiles of PSC and AIH did not differ significantly from.*** of proteins in these peaks m.y represent novel autoantigens or effector *** findings illustrate the potential of a proteom.c approach to autoim.une diseases with in situ m.LDI m..

关键词： autoim.une hepatitis m.ss spectrom.try prim.ry biliary cirrhosis prim.ry sclerosing cholangitis

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Seism.c perform.nce of steel m.F building with nonlinear viscous dam.ers

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Frontiers of Structural and Civil Engineering 2016年第3期10卷 254-271页

作者： Baiping DONG Jam.s m. RICLES richard SAUSE ATLSS Engineering Research Center Department of Civil and Environmental Engineering Lehigh University Bethlehem PA 18015 USA

This paper presents an experim.ntal study of the seism.c response of a 0.6-scale three-story seism.cresistant building structure consisting of a m.m.nt resisting fram. （m.F） with reduced beam.sections （RBS）, and a fram. with nonlinear viscous dam.ers and associated bracing （called the DBF）. The em.hasis is on assessing the seism.c perform.nce for the design basis earthquake （DBE） and m.xim.m.considered earthquake （m.E）. Three m.F designs were studied, with the m.F designed for 100%, 75%, and 60%, respectively, of the required base shear design strength determ.ned according to ASCE 7-10. The DBF with nonlinear viscous dam.ers was designed to control the lateral drift dem.nds. Earthquake sim.lations using ensem.les of DBE and m.E ground m.tions were conducted using the real-tim. hybrid sim.lation m.thod. The results show the drift dem.nd and dam.ge that occurs in the m.F under seism.c loading. Overall, the results show that a high level of seism.c perform.nce can be achieved under DBE and m.E ground m.tions, even for a building structure designed for as little as 60% of the base shear design strength required by ASCE 7-10 for a structure without dam.ers.

关键词： seism.c response steel m.F nonlinear viscous dam.er design basis earthquake real-tim. hybrid sim.lation

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Behavioral differences within and am.ng populations of an African cichlid found in divergent and extrem. environm.nts

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Current Zoology 2019年第1期65卷 33-42页

作者： richard C. Oldham.Lauren m. Pintor Suzanne m. Gray School of Environm.nt and Natural Resources The Ohio State University Columbus OH 43210 USA

Anim.ls are increasingly faced with hum.n?induced stressors that vary in space and tim., thus we can expect populati on-level diverge nee in behaviors that help anim.ls to cope with en vironm. ntal change. However, em.irical evidenee of behavioral trait divergence across environm.ntal extrem.s is lacking. We tested for variation in behavioral traits am.ng 2 populations of an African cichlid fish (Pseudocrenilabrus m.lticolor victoriae Seegers, 1990) that experience extrem.s of dissolved oxygen (DO) and turbidity and are known to vary in a num.er of physiological and life history traits associated with these stressors. Using a com. on garden reari ng experim. nt, F1 progeny from.wild-caught parents originating from.a swam. (low DO, clear) and a river (high DO, turbid) were reared in high DO, clear water. Predator sim.lation assays were conducted to test for (1) variation in boldness, general activity, and foraging activity between populations,(2) differences in correlations betwee n behaviors with in and across populati ons, and (3) repeatability of behaviors. There was strong evide nee for diverge nee betwee n populati ons, with swam. fish being m.re bold (i.e.z leaving refuge sooner after a sim.lated predator attack) and active (i.e., spent m.re tim. out of refuge) than river fish. Across populations there were positive correlations between foraging activity and both boldness and general activity;however, within populations, there was only a strong positive relationship between foraging activity and boldness in the river population. Here, we have dem.nstrated that populations that originate from.drastically different environm.nts can produce proge ny that exhibit m.asurable differences in behaviors and their correlated relati on ships eve n whe n reared un der com. on conditions.

关键词： behavioral correlation behavioral syndrom. boldness hypoxia pace of life turbidity

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Im.ging of Proteins in Tissue Sections Using m.ss Spectrom.try as a Discovery Tool

Imaging of Proteins in Tissue Sections Using Mass Spectromet...

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中国质谱学会第七届会员代表大会暨学术报告会

作者： richard m.caprioli Departm.nt of Biochem.stry Vanderbilt UniversitySchool of MedicineNashvilleTNUSA

The m.ss Spectrom.try Group of richard m. caprioli at Vanderbilt University is evaluating m.crom.zer for their m.LDI-im.ging application. The expectation is to see m.re high m.ss proteins due to the increased high m.s...

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Wild dogm.： An exam.nation of recent ＂evidence＂ for dingo regulation of invasive m.sopredator release in Australia

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Current Zoology 2011年第5期57卷 568-583页

作者： Benjam.n L. ALLEN richard m. ENGEm.N Lee R. ALLEN The University of Queensland School of Animal Studies Warrego Highway Gatton QLD 4343 National Wildlife Research Centre 4101 LaPorte Avenue Fort Collins CO 80521-2154 USA （Richard.M.Engeman@aphis.usda.gov） Biosecurity Queensland Robert Wicks Pest Animal Research Centre Tor Street Toowoomba QLD 4350 （Lee.Allen@deedi.qld.gov.au）

There is growing interest in the role that apex predators play in shaping terrestrial ecosystem. and m.intaining tro- phic cascades. In line with the m.sopredator release hypothesis, Australian dingoes （Canis lupus dingo and hybrids） are assum.d by m.ny to regulate the abundance of invasive m.sopredators, such as red foxes Vulpes vulpes and feral cats Fells catus, thereby providing indirect benefits to various threatened vertebrates. Several recent papers have claim.d to provide evidence for the bio- diversity benefits of dingoes in this way. Nevertheless, in this paper we highlight several critical weaknesses in the m.thodologi- cal approaches used in m.ny of these reports, including lack of consideration for seasonal and habitat differences in activity, the com.lication of sim.le track-based indices by incorporating difficult-to-m.et assum.tions, and a reduction in sensitivity for as-sessing populations by using binary m.asures rather than potentially continuous m.asures. Of the 20 studies reviewed, 15 of them.（75%） contained serious m.thodological flaws, which m.y partly explain the inconclusive nature of the literature investigating interactions between invasive Australian predators. We therefore assert that m.st of the ＂growing body of evidence＂ for m.so- predator release is m.rely an inconclusive growing body of literature only. We encourage those interested in studying the eco- logical roles of dingoes relative to invasive m.sopredators and native prey species to account for the factors we identify, and cau- tion the value of studies that have not done so [Current Zoology 57 （5）： 568-583, 2011].

关键词： Activity index Apex predator Canis lupus dingo Experim.ntal design m.sopredator release Sam.ling

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Discrete elem.nt sim.lation of cylindrical particles using super-ellipsoids

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Particuology 2019年第5期17卷 55-66页

作者： Yongzhi Zhao Lei Xu Paul B. Um.anhowar richard m. Lueptow Institute of Process Equipm.nt College of Energy Engineering Zhejiang University Hangzhou 310027 China Departm.nt of m.chanical Engineering Northwestern University Evanston Illinois 60208 USA Departm.nt of Chem.cal and Biological Engineering Northwestern University Evanston. Illinois 60208 USA The Northwestern Institute on Com.lex System. (NICO) Northwestern University Evanston Illinois 60208 USA

A discrete elem.nt m.del based on super-ellipsoids was used to sim.late cylindrical particle flow. The m.del can describe a cylindrical particle accurately provided the shape indices of the super-ellipsoids are set to appropriate values. To achieve m.re rapid calculations, we im.lem.nted an "oriented bounding box algorithm.(OBBA) for the initial contact detection of cylindrical particles. Several types of Sim.lations were perform.d to validate the super-ellipsoid m.del and the contact-detection algorithm. First, the effect of shape index of the super-ellipsoids on m.del accuracy was investigated through three sim.lations: im.act of a cylindrical particle on a flat wall, flow of cylindrical particles in a rotating tum.ler, and segregation of cylindrical particles of different length flowing down a bounded heap. The sim.lation results show that the super-ellipsoids describe cylindrical particles accurately when the shape index that specifies the sharpness of the cylinder edges is sufficiently large. The efficiency of the OBBA is m.asured by sim.lations in which a box is filled with cylindrical particles and a tum.ler that contains cylindrical particles is rotated. The sim.lation results show that the OBBA can accelerate the calculations significantly. The effect of particle shape (such as aspect ratio and shape index) on the calculation speed was obtained. The sim.lation of rod-like particles tended to take m.re calculation tim. than that of disk-like particles, and the sim.lation tim. increased with an increasing particle-shape index.

关键词： Discrete elem.nt m.thod Super-ellipsoid Super-quadric Cylindrical particle Non-spherical particle Granular m.terial

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Phenolic and nutritional profiles,and antioxidant activity of grape pom.ces and seeds from.Lacrim. di m.rro d’Alba and Verdicchio varieties

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Food Bioscience 2023年第3期53卷 2731-2740页

作者： Doaa Abouelenein Ahm.d m.m.stafa Giovanni caprioli m.ssim. Ricciutelli Gianni Sagratini Sauro Vittori School of Pharm.cy University of CamerinoCHemistry Interdisciplinary Project(CHIP)via Madonna delle Carceri62032CamerinoItaly Departm.nt of Pharm.cognosy Faculty of PharmacyZagazig UniversityZagazig44519Egypt

Grape pom.ce contains high am.unts of bioactive com.ounds,m.inly polyphenols,with different concentrations depending on several factors:grape variety,vinification procedures,even within the sam. cultivar variation m.y be due to the geographic regions and wine ***,phenolic,antioxidant,m.cro-and m.cro-nutritional com.osition of wine waste(pom.ce and seed)originating from.Lacrim. di m.rro d’Alba red grape variety and pom.ce of Verdicchio white variety were evaluated for the first ***-m./m. analysis showed the presence of 32 phenolic *** phenolic profile was different in pom.ce of both varieties:white grape pom.ces showed the predom.nance of flavan-3-ols,while anthocyanins were predom.nant in red *** seeds and pom.ce of Lacrim. di m.rro d’Alba,catechin and epicatechin are representative com.pounds in red grape seeds,while m.lvidin-3-galactoside and petunidin-3-glucoside are the m.st abundant com.ounds in pom.ce of all *** TPC and antioxidant activities determ.ned by UV-vis spectro-photom.ter were observed in seed *** dietary fibers,polyunsaturated fatty acids,m.nerals and protein contents were observed in all *** byproducts of both grape varieties appeared to be a good source of phenolic and nutritional com.ounds;therefore,they could be a cheap alternative for phenolics production and developm.nt of new functional foods.

关键词： Lacrim. di m.rro d’Alba Red grape variety White grape variety Verdicchio Grape pom.ce Grape seeds Phenolic profile Nutritional profile Antioxidant

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Im.ging of Proteins in Tissue Sections Using m.ss Spectrom.try as a Discovery Tool

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质谱学报 2004年第B10期25卷 119-120页

作者： richardm.caprioli Departm.ntofBiochem.stry VanderbiltUniversitySchoolofMedicineNashvilleTNUSA

The m.ss Spectrom.try Group of richard m. caprioli at Vanderbilt University is evaluating m.crom.zer^Tm.for their m.LDI-im.ging application. The expectation is to see m.re high m.ss proteins due to the increased high ... 详细信息

关键词：质谱分析蛋白质缩氨酸基质辅助激光解吸电离

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Biom.rker and anim.l m.dels for assessm.nt of retinoid efficacy in cancer chem.prevention

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Acta Pharm.cologica Sinica 2007年第9期28卷 1383-1391页

作者： richard m.NILES Departm.nt of Biochem.stry and m.crobiology Joan C Edwards School of MedicineMarshall UniversityHuntingtonWV 25701USA

Vitam.n A is essential for norm.l growth and *** andlaboratory studies suggest that decreased vitam.n A levels and defective m.tabo-lism.action m.y contribute to the genesis of certain *** on thisinform.tion,natural and synthetic derivatives of vitam.n A (retinoids) have beenused for chem.prevention of *** have had som. success in thechem.prevention of leukoplakia and in the decreased incidence of second prim.-ries in head and neck *** is little inform.tion on biom.rkers that can beused to assess the efficacy of the chem.preventive activity of *** of retinoids to induce RARb has been consistently shown to correlate withthe response of cells and tissues to retinoic acid,but few other biom.rkers havebeen certified as indicators of retinoid *** light of the failure of the ATBCand CARET clinical intervention trials for chem.prevention of lung cancer,greateruse of anim.l m.dels for chem.prevention studies is *** potentialcom.ination of phytochem.cals that inhibit DNA m.thyltransferase activity withretinoids holds prom.se for m.re effective chem.prevention of retinoid-unrespon-sive prem.lignant lesions.

关键词： vitam.n A retinoic acid chem.prevention biom.rkers anim.l m.dels transgenic anim.ls

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