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Effects of B[a]P-UVA Co-Exposure on Epigenetic Marks of Isolated Skin Cells and Impact of an Arundo donax L. Extract of These Changes

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Journal of Cosmetics, Dermatological Sciences and Applications 2024年第3期14卷 253-269页

作者： Marie M. Dorr Magali Favre-Mercuret Katell Vié richard Fitoussi Laboratoires Clarins Pontoise France

Background: Extrinsic aging results from environmental stressors such as UVR or pollutants. While the effects of single pollutants are better understood, those of their combination remain poorly scrutinized. Objective: Building on a study showing downregulation of several processes upon co-exposure to B[a]P and UVA, we investigated changes induced by epigenetic marks. Materials and Methods: Human primary fibroblasts and HaCaT cells were exposed to B[a]P and UVA. After 24 hours, exposed and unexposed cells were compared to assess DNA methylation. Focusing on HaCaT, multiplex assays enabled quantifying histone H3 modifications and evaluating four splicing factors (SRSF1, SRSF3, SFPQ, and SF3B1) by immunohistochemical labeling. The expression of keratinocyte-/fibroblast-relevant genes was assessed by RT-qPCR. Finally, the impact of an Arundo donax L. extract added 24 hours before B[a]P-UVA co-exposure was analyzed. Results: Exposure to B[a]P-UVA raised DNA methylation (HaCaT: ×3.6, fibroblasts: ×1.9), an increase prevented by the extract. In HaCaT cells, B[a]P-UVA increases the frequency of S10P (+38%). When exposure was preceded by extract treatment, the frequency of several methylations was impacted. B[a]P-UVA only induced the expression of SRSF1 and SFPQ in HaCaT (+46% and +34%). Treatment with the extract abolished this effect. Co-exposure increases the expression of inflammation-related genes (IL-1α, IL-1β) in HaCaT cells and decreases those of AQP3, KRT15, and SOD2. The extract has little effect on these changes. In primary fibroblasts, exposure to B[a]P-UVA lowered the expression of LOXL2, LUM, and TGFBR2 (−38%, −59%, and −51%, respectively), and the extract did not affect these modifications. Conclusion: Within 24 hours, a single B[a]P-UVA co-exposure changes epigenetic marks of skin cells but has only mild effects on gene expression. An Arundo donax L. extract can prevent part of the epigenetic marks’ changes and could stimulate the expression of some genes in

关键词： Extrinsic Aging DNA Methylation Histone H3 Splicing Factors Arundo donax L.

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Monogenic deficiency in murine intestinal Cdc42 leads to mucosal inflammation that induces crypt dysplasia

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Genes & Diseases 2024年第1期11卷 413-429页

作者： Dongsheng Zhang Wenjuan Tang Haitao Niu William Tse Hai-Bin Ruan Helmut Dolznig Thomas Knosel Friedrich Karl-Heinz Madeleine Themanns Jiang Wang Mingquan Song Lee Denson Lukas Kenner richard Moriggl Yi Zheng Xiaonan Han Division of Hematology and Oncology Division of Cancer BiologyDepartment of MedicineMetroHealth Medical Center(MHMC)Case Western Reserve University(CWRU)School of MedicineClevelandOH 44109USA Cancer Genomics and Epigenomics Program Case Comprehensive Cancer CenterCase Western Reserve University(CWRU)ClevelandOH 44106USA Division of Gastroenterology Hepatology and NutritionCincinnati Children’s Hospital Medical Center(CCHMC)CincinnatiOH 45229USA Children’s Hospital of Fudan University Shanghai 201102China School of Medicine Jinan UniversityGuangzhouGuangdong 510632China Laboratory Animal Science(ILAS) Chinese Academy of Medical Science(CAMS)and Peking Union Medical College(PUMC)Beijing 100006China Department of Integrative Biology and Physiology University of Minnesota Medical SchoolMinneapolisMI 55455USA Institute of Medical Genetics Medical University of ViennaVienna 1040Austria Institute of Pathology Ludwig-Maximilians-University MunichMunich 80539Germany Institute of Biochemistry II University Hospital JenaJena 07743Germany Laboratory Animal Pathology University of Veterinary Medicine ViennaVienna 1210Austria Department of Pathology University of CincinnatiCincinnatiOH 45221USA Department of Gastroenterology The Affiliated Hospital of Qingdao UniversityQingdaoShandong 266005China Department of Pathology Medical University of ViennaVienna 1040Austria Ludwig Boltzmann Institute for Cancer Research Vienna 1090Austria Medical University of Vienna Vienna 1040Austria Institute of Animal Breeding and Genetics University of Veterinary Medicine ViennaVienna 1210Austria Division of Experimental Hematology CCHMCCincinnatiOH 45229USA

CDC42 controls intestinal epithelial(IEC)stem cell(IESC)*** aberrant CDC42 initiates intestinal inflammation or neoplasia is *** utilized models of inflam-matory bowel diseases(IBD),colorectal cancer,aging,and IESC injury to determine the loss of intestinal Cdc42 upon inflammation and *** specimens were collected to determine the levels of CDC42 in IBD or colorectal ***42 floxed mice were crossed with Villin-Cre,Villin-CreERT2 and/or Lgr5-eGFP-IRES-CreERT2,or Bmi1-CreERT2 mice to generate Cdc42 deficient ***,colitis,aging,and intestinal organoid were used to evaluate CDC42 upon mucosal inflammation,IESC/progenitor regenerative capacity,and IEC *** studies revealed that increased CDC42 in colorectal cancer correlated with lower survival;in contrast,lower levels of CDC42 were found in the inflamed IBD *** Cdc42 depletion significantly reduced Lgr5+IEsCs,increased progenitors'hyperplasia,and induced mucosal inflammation,which led to crypt *** Cdc42 depletion markedly enhanced irra-diation-or chemical-induced *** or inhibition of Cdc42 reduced colonic Lgr5+IESC *** conclusion,depletion of Cdc42 reduces the IESC regeneration and IEC repair,leading to prolonged mucosal *** monogenic loss of Cdc42 in-duces mucosal inflammation,which could result in intestinal neoplasia in the context of aging.

关键词： Cell divisioncycle 42(CDC42) Colitis Colorectal cancer(CRC) Inflammatory bowel diseases(IBD) Intestinal epithelial cell(IEC) Intestinal epithelial stem cell(IESC) Irradiation

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COVID-19 vaccination produces exercise-responsive SARS-CoV-2 specific T-cells regardless of infection history

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Journal of Sport and Health Science 2024年第1期13卷 99-107页

作者： Kyle A.Smith Tiffany M.Zúñiga Forrest L.Baker Helena Batatinha Charles R.Pedlar Shane C.Burgess Michael P.Gustafson Emmanuel Katsanis richard J.Simpson School of Nutritional Sciences and Wellness The University of ArizonaTucsonAZ 85721USA Department of Pediatrics The University of ArizonaTucsonAZ 85724USA Faculty of Sport Allied Health and Performance ScienceSt.Mary's UniversityTwickenham TW14SXUK Institute of Sport Exercise and Health University College LondonLondon WC1E 7HUUK Department of Animal and Comparative Biomedical Sciences The University of ArizonaTucsonAZ 85721USA The University of Arizona Cancer Center The University of ArizonaTucsonAZ 85719USA Laboratory Medicine and Pathology Mayo Clinic ArizonaPhoenixAZ 85054USA Department of Immunobiology The University of ArizonaTucsonAZ 85724USA Department of Medicine The University of ArizonaTucsonAZ 85724USA Department of Pathology The University of ArizonaTucsonAZ 85724USA

Background:The mobilization and redistribution of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)specific T-cells and neutralizing antibodies(nAbs)during exercise is purported to increase immune surveillance and protect against severe coronavirus disease 2019(COVID-19).We sought to determine if COVID-19 vaccination would elicit exercise-responsive SARS-CoV-2 T-cells and transiently alter nAb ***:Eighteen healthy participants completed a 20-min bout of graded cycling exercise before and/or after receiving a COVID-19 *** major leukocyte subtypes were enumerated before,during,and after exercise by flow cytometry,and immune responses to SARS-CoV-2 were determined using whole blood peptide stimulation assays,T-cell receptor(TCR)-βsequencing,and SARS-CoV-2 nAb ***:COVID-19 vaccination had no effect on the mobilization or egress of major leukocyte subsets in response to intensity-controlled graded ***,non-infected participants had a significantly reduced mobilization of CD4+and CD8+naive T-cells,as well as CD4+central memory T-cells,after vaccination(synthetic immunity group);this was not seen after vaccination in those with prior SARS-CoV-2 infection(hybrid immunity group).Acute exercise after vaccination robustly mobilized SARS-CoV-2 specific T-cells to blood in an intensity-dependent *** groups mobilized T-cells that reacted to spike protein;however,only the hybrid immunity group mobilized T-cells that reacted to membrane and nucleocapsid *** increased significantly during exercise only in the hybrid immunity ***:These data indicate that acute exercise mobilizes SARS-CoV-2 specific T-cells that recognize spike protein and increases the redistribution of nAbs in individuals with hybrid immunity.

关键词： Anti-viral COVID-19 Exercise immunology SARS-CoV-2 T-Cells Vaccine

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Forest Entomology and Pathology:Volume 1:Entomology

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Entomotaxonomia 2024年第3期46卷 264-264页

作者： John richard Schrock 不详

Released July 1, 2023 by Springer, this 822 page book in English consists of 23 chapters. Chapters 1–8 address basic entomology concepts from morphology and function to general insect ecology, population dynamics, in... 详细信息

关键词： Entomology Forest forest

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Dynamics of Potable Well Water Quality in Key Mining Chiefdoms in Kono District, Eastern Sierra Leone

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Open Journal of Applied Sciences 2024年第7期14卷 1927-1943页

作者： richard Tamba Simbo Alhaji Brima Gogra Yahaya Kudus Kawa Juana Paul Moiwo Department of Science and Mathematics College of Arts and Sciences William V.S. Tubman University Harper Liberia Port Loko University College Campus Ernest Bai Koroma University of Science and Technology Port Loko Sierra Leone Department of Chemistry School of Basic Sciences Njala University Bo Sierra Leone Department of Agricultural Engineering School of Technology Njala University Bo Sierra Leone

Groundwater is increasingly being used due to its universal availability and generally good quality. However, the risk of contamination of groundwater due to various human activities such as mining is equally increasing across the globe. In this study, the physical parameters of potable well waters in the key mining areas in Nimikoro and Tankoro Chiefdoms in Kono District were analyzed for compliance with drinking water quality standard. To do this, both unpurged and purged well water samples were collected once every month for a period of one year. Some of the well water properties like temperature, Total Dissolved Solids (TDS) and Electrical Conductivity (EC) were measured on site and others determined in the laboratory. The data collected from the laboratory analyses were statistically analyzed in MS Excel, SPSS and ArcGIS environments for quality trends in time-space fabric. The results showed that well water quality in the study area generally fell short of drinking water quality standards of Sierra Leone and WHO. There were high temperature and turbidity during the dry season and then high TDS and EC during the rainy season. Temperature and turbidity also significantly influenced well water quality in the study area, much more than TDS and EC. The implications for drinking water of lower quality than the standard could be huge for the local population and therefore needs the attention of stakeholders in the study area and decision makers in the country.

关键词： Temperature Total Dissolved Solids Turbidity Electrical Conductivity Water Quality

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Analysis of the Characteristics of Materials Obtained by Recycling Scrap Metal in Guinea

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材料科学与工程（中英文A版） 2024年第1期14卷 1-10页

作者： Alpha Issa Diallo Jean Ahmed Kourouma Adama Moussa Sakho Alexandre Lucien richard Mechanical Laboratory Higher Institute of Technology of MamouMamou 063Republic of Guinea Materials Laboratory of Gamal Abdel Nasser University ConakryMamou1147Republic of Guinea Department of Laboratory Technology Higher Institute of Technology of MamouMamou 063Republic of Guinea

The aim of this study was to determine the quality of rebar produced from recycled scrap metal collected throughout the country,and imported rebar sold in the Republic of *** do this,the samples were subjected to various mechanical tests involving traction,bending and microscopic *** the Lambanyi and Casse Sonfonia samples,all the tensile strength values for diameters 12,14 and 16 were above 550 ***,the iron samples from Baillobaye and the 10 mm diameters of the samples from Casse Sonfonio and Lambanyi have less appreciable *** limits of elasticity were *** various values found vary more or less from the conventional yield strength of the NF A35-016 reference supplied by CBITEC,which is 500 *** analysis gives us an insight into the internal structure of the iron samples *** study may provide the company and the vendors with an alternative for their improvements.

关键词： Steel in construction recycled scrap strength test traction bending.

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Mesh representation matters:investigating the influence of different mesh features on perceptual and spatial fidelity of deep 3D morphable models

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虚拟现实与智能硬件（中英文） 2024年第5期6卷 383-395页

作者： Robert KOSK richard SOUTHERN Lihua YOU Shaojun BIAN Willem KOKKE Greg MAGUIRE Centre for Digital Entertainment National Centre for Computer AnimationBournemouth UniversityPoole BH125BBUK Humain Ltd. Belfast BT12LAUK School of Creative and Digital Industries Buckinghamshire New UniversityHigh Wycombe HP112JZUK Belfast School of Art Ulster UniversityBelfast BT151EDUK

Background Deep 3D morphable models(deep 3DMMs)play an essential role in computer *** are used in facial synthesis,compression,reconstruction and animation,avatar creation,virtual try-on,facial recognition systems and medical *** applications require high spatial and perceptual quality of synthesised *** their significance,these models have not been compared with different mesh representations and evaluated jointly with point-wise distance and perceptual *** We compare the influence of different mesh representation features to various deep 3DMMs on spatial and perceptual fidelity of the reconstructed *** paper proves the hypothesis that building deep 3DMMs from meshes represented with global representations leads to lower spatial reconstruction error measured with L_(1) and L_(2) norm metrics and underperforms on perceptual *** contrast,using differential mesh representations which describe differential surface properties yields lower perceptual FMPD and DAME and higher spatial fidelity *** influence of mesh feature normalisation and standardisation is also compared and analysed from perceptual and spatial fidelity *** The results presented in this paper provide guidance in selecting mesh representations to build deep 3DMMs accordingly to spatial and perceptual quality objectives and propose combinations of mesh representations and deep 3DMMs which improve either perceptual or spatial fidelity of existing methods.

关键词： Shape modelling Deep 3D morphable models Representation learning Feature engineering Perceptual metrics

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Social and Environmental Impacts of Maryland Oil Palm Plantations on Forests,Biodiversity,and Community Livelihoods in Liberia

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Journal of Agricultural Science and Technology(A) 2024年第2期14卷 83-92页

作者： richard N.Sam Sampson Williams Nornor N.Bee Dioh Flahn Community Rights and Corporate Governance Program Sustainable Development Institute(SDI)DuarzonRobert Field HighwayMargibi10 Liberia

Liberia holds 44.5% of the remaining portion of the Upper Guinean Rainforest in West Africa,which is home to critically endangered forest elephants and western *** forests are of vital importance for the livelihoods of millions of West Africans and provide key ecosystem services of local and global importance for food systems transformation and ***’s efforts toward land reform through legislation and policies recognise communities’rights to own and manage their customary lands and *** include the National Forestry Reform Law of 2006,the Community Rights Law Concerning Forest Lands of 2009,and the Land Rights Act of 2018,and *** May 2022,a program team from the Sustainable Development Institute(SDI)-Friends of the Earth Liberia researched the social and environmental impacts of Maryland Oil Palm Plantations(MOPPs)in ***-three(23)key informant interviews(KIIs)and 10 focus group discussions(FGDs)were conducted in seven communities in and around the *** included farmers,contract workers,MOPP staff,local authorities,women and youth leaders,the Environmental Protection Agency(EPA)Inspector,the Civil Society Head,and the Gender Coordinator of Maryland *** team cross-checked information with formal documents as much as possible and took photographs and global positioning system(GPS)locations of areas of deforestation,pollution,and *** team also used observation to monitor environmental pollution,such as affluent into water bodies and planting oil palm in *** team used narrative analysis and geospatial landscape analysis to analyze the *** research finds that land conflict and deforestation have several negative impacts on *** has not respected land tenure rights or followed Free Prior and Informed Consent(FPIC)standards,including resettlement without reparation and destruction of farms and old towns without(sufficient)compensation or *** MOPP land acquisiti

关键词： Biodiversity climate change community rights deforestation ecosystem services food security human rights land rights forest rights livelihood

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细胞壁降解酶在球刺盘孢侵染过程中的作用

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植物保护 2024年第3期50卷 88-98,110页

作者：金梦军杨成德李统华 OSEI richard 蔡锋锋马婷甘肃省农作物病虫害生物防治工程实验室甘肃农业大学植物保护学院兰州730070

为明确球刺盘孢Colletotrichum coccodes产生的细胞壁降解酶(CWDEs)在其侵染马铃薯过程中的作用,本文对球刺盘孢离体和活体条件下产生的CWDEs种类及活性进行检测,并通过RT-qPCR测定了球刺盘孢侵染过程中多聚半乳糖醛酸酶(PG)基因的表达... 详细信息

为明确球刺盘孢Colletotrichum coccodes产生的细胞壁降解酶(CWDEs)在其侵染马铃薯过程中的作用,本文对球刺盘孢离体和活体条件下产生的CWDEs种类及活性进行检测,并通过RT-qPCR测定了球刺盘孢侵染过程中多聚半乳糖醛酸酶(PG)基因的表达情况。通过定性测定发现,球刺盘孢能够产生果胶酶、纤维素酶和蛋白酶;定量测定发现,球刺盘孢可产生羧甲基纤维素酶(Cx)、β-葡萄糖苷酶(β-Glu)、PG和聚甲基半乳糖醛酸酶(PMG)。在活体条件下,球刺盘孢可在马铃薯茎秆内产生PG、PMG和多聚半乳糖醛酸反式消除酶(PGTE),且酶活分别于接种后48、72 h和144 h达到最大值,分别为26.50、77.61 U/mL和0.16 U/mg。分别将羧甲基纤维素钠和果胶诱导后的酶液接种至马铃薯茎秆可引起植物组织浸腐,与球刺盘孢引起的典型症状相似。除此之外,球刺盘孢的4个PGs基因均在其侵染后24 h和48 h显著共上调表达。综上所述,该研究结果表明,果胶酶在球刺盘孢致病过程中发挥重要作用。

关键词：球刺盘孢马铃薯细胞壁降解酶多聚半乳糖醛酸酶

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Preclinical and clinical evidence of the association of colibactinproducing Escherichia coli with anxiety and depression in colon cancer

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World Journal of Gastroenterology 2024年第21期30卷 2817-2826页

作者： Fabien Rondepierre Maëva Meynier Johan Gagniere Vincent Deneuvy Anissa Deneuvy Gwenaelle Roche Elodie Baudu Bruno Pereira richard Bonnet Nicolas Barnich Frédéric Antonio Carvalho Denis Pezet Mathilde Bonnet Isabelle Jalenques Service de Psychiatrie de l’Adulte A et Psychologie Médicale CHU Clermont-FerrandClermont-Ferrand 63000France M2iSH UMR 1071INSERMUniversity of Clermont AuvergneINRAE USC 1382Clermont-Ferrand 63001France NeuroDol UMR 1107INSERMUniversity of Clermont AuvergneClermont-Ferrand 63001France Department of Digestive Surgery CHU de Clermont-FerrandClermont-Ferrand 63001France Biostatistics Unit Department of Clinical Research and InnovationCHU Clermont-FerrandClermont-Ferrand 63000France Department of Bacteriology CHU de Clermont-FerrandClermont-Ferrand 63001France UniversitéClermont Auvergne INPCNRSClermont Auvergne Institut PascalClermont-Ferrand 63000France

BACKGROUND The association between the intestinal microbiota and psychiatric disorders is becoming increasingly *** gut microbiota contributes to colorectal carcinogenesis(CRC),as demonstrated with colibactin-producing Escherichia coli(CoPEC).AIM To evaluate the association between CoPEC prevalence and anxiety-and depressive-like behaviors with both preclinical and clinical *** Patients followed after a CRC surgery and for whom the prevalence of CoPEC has been investigated underwent a psychiatric *** were compared according to the CoPEC *** parallel C57BL6/J wild type mice and mice with a CRC susceptibility were chronically infected with a CoPEC *** behavior was assessed using the Elevated Plus Maze test,the Forced Swimming Test and the Behavior recognition system PhenoTyper®.RESULTS In a limited cohort,all patients with CoPEC colonization presented with psychiatric disorders several years before cancer diagnosis,whereas only one patient(17%)without CoPEC *** result was confirmed in C57BL6/J wildtype mice and in a CRC susceptibility mouse model(adenomatous polyposis colimultiple intestinal neoplasia/+).Mice exhibited a significant increase in anxiety-and depressive-like behaviors after chronic infection with a CoPEC *** This finding provides the first evidence that CoPEC infection can induce microbiota-gut-brain axis disturbances in addition to its procarcinogenic properties.

关键词： Colorectal cancer Colibactin Escherichia coli Colibactin-producing Escherichia coli Inflammation Anxiety Depression Behavior

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