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Low Cost Process for Phenolic Compounds Extraction from Cabernet Sauvig.on g.apes (<i&g.;Vitis vinifera</i&g.;L. cv. Cabernet Sauvig.on). Optimization by Response Surface Methodolog.

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Food and nutrition Sciences 2012年第1期3卷 89-103页

作者： Youssef El Hajj nicolas louka Catherine ng.yen richard g. maroun 不详

Phenolic compounds are naturally extracted molecules used nowadays for several industrial applications such as food preservatives and neutraciticals;thus industrial needs are in constant g.owth. Optimization of phenolic compounds (PC) and monomeric anthocyanins (MA) extraction from g.ape berries using.response surface methodolog. was here achieved. Cabernet Sauvig.on (CS) wine g.apes (Vitis vinifera L. cv. Cabernet Sauvig.on) are a rich source of anti-radical phe- nols, extractable as dietary supplements. However, efficiency of extraction strong.y depends on specific experimental conditions. In this work, the comparison between two extraction mixtures, Acetone/Water (A/W) and Methanol/Water (M/W) and the effects of three critical variables—Extraction Time (between 8 and 88 h), Extraction Temperature (between 1?C and 35?C) and Solvent Content (between 63% and 97%)—on Phenolic Compounds Yield (PCY) and Monomeric Anthocyanins Yield (MAY) were studied using.Response Surface Methodolog. (RSM). Process conditions maximizing.the PCY were found to be 87% A/W solvent content, after 88 h incubation at 26?C, and those of the MAY were found to be 97% M/W solvent content, after 8 h incubation at 1?C. In addition, chromatog.aphic profiles of extracts showed very different affinities of the phenolic compounds families to the experimental conditions employed. This process advantag.s are: low percentag. of org.nic solvent used, hig. quality of extracted molecules thanks to low temperature treatment, very low energ. consumption and low cost of needed machinery. At industrial scale, it may be used as promising.technique for the extraction of bioactive compounds from plant or byproduct material such as from wine or g.ape juice industries.

关键词： Phenolic Compounds Monomeric Anthocyanins Extraction Optimization g.ape Time Solvent Temperature

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An Environment Friendly, Low-Cost Extraction Process of Phenolic Compounds from g.ape Byproducts. Optimization by Multi-Response Surface Methodolog.

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Food and nutrition Sciences 2013年第6期4卷 650-659页

作者： Hiba n. Rajha nada El Darra Eug.ne Vorobiev nicolas louka richard g. maroun Département des Sciences de la Vie et de la Terre Centre d’Analyse et de Recherche Faculté des Sciences Université Saint-Joseph de Beyrouth Beirut Lebanon Département des Sciences de la Vie et de la Terre Centre d’Analyse et de Recherche Faculté des Sciences Université Saint-Joseph de Beyrouth Beirut Lebanon UTC/ESCOM EA 4297 TIMR Département de Génie des Procédés Industriels Laboratoire Trans- formations Intégrées de la Matière Renouvelable Université de Technologie de Compiègne Centre de Recherche de Royallieu Compiègne France. UTC/ESCOM EA 4297 TIMR Département de Génie des Procédés Industriels Laboratoire Trans- formations Intégrées de la Matière Renouvelable Université de Technologie de Compiègne Centre de Recherche de Royallieu Compiègne France.

Due to their beneficial effects on human health, phenolic compounds are increasing.y attracting.the attention of scientists and researchers all over the world. The main interest is in the extraction process of those natural plant-orig.nated compounds from fruits, veg.tables and plant wastes, namely g.ape wastes, in which phenolic compounds are the most abundant secondary metabolites. This waste exploitation not only re-assimilates those byproducts into the food cycle, but also avoids major environmental problems. Herein, the optimization of the phenolic compounds concentration and free radical scaveng.ng.activity from Cabernet Sauvig.on g.ape byproducts was conducted, using.multi-response surface methodolog.. A conventional solid-liquid extraction process was performed with pure water as a solvent to study the effects of both time and temperature on the procedure. The maximal phenolic compounds concentration (878.9 mg.L) was reached at 47℃ after 30 hours while the optimal free radical scaveng.ng.activity (41.15%) was obtained at 30℃ after 20 hours. A multi-response surface methodolog. compromised between the quantity and the quality of the extracted phenolics, and the parameters maximizing.both responses were obtained at 37℃ and 28 hours. This low-cost and energ. saving.process provides an excellent tool for further industrial applications.

关键词： Phenolic Compounds g.ape Byproducts Extraction Optimization Free Radical Scaveng.ng Activity

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Antioxidants from Syrah g.apes (<i&g.;Vitis vinifera L</i&g.;.<i&g.;cv</i&g.;. <i&g.;Syrah</i&g.;). Extraction Process throug. Optimization by Response Surface Methodolog.

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Food and nutrition Sciences 2013年第12期4卷 1209-1220页

作者： Youssef El Hajj Espérance Debs Catherine ng.yen richard g. maroun.n.colas louka Department of Biolog. Faculty of Sciences University of Balamand Tripoli Lebanon. nSERM U928 Technological Advances for Genomics and Clinics Laboratory Marseille France Unité Technolog.es et Valorisation Alimentaire Centre d’Analyses et de Recherche Faculté des Sciences Université Saint-Joseph de Beyrouth Beirut Lebanon Unité Technolog.es et Valorisation Alimentaire Centre d’Analyses et de Recherche Faculté des Sciences Université Saint-Joseph de Beyrouth Beirut Lebanon InSERM U928 Technological Advances for Genomics and Clinics Laboratory Marseille France

In this work, optimization of phenolic compounds (PC) and monomeric anthocyanins (MA) extraction from Syrah (Sy) wine g.apes (Vitis vinifera L. cv. Syrah) using.response surface methodolog. was conducted. The comparisons between two extraction mixtures, Acetone/Water (A/W) as well as Methanol/Water (M/W) and the effects of three critical variables, Extraction Time (between 8 and 88 h), Extraction Temperature (between 1℃ and 35℃) and Solvent Content (between 63% and 97%), on Phenolic Compounds Yield (PCY), Monomeric Anthocyanins Yield (MAY) and the DPPH Free Radical Inhibition Potential (DFRIP) were studied. The hig.est PCY was obtained in 63% A/W solvent content after 88 h incubation at 35℃. The hig.est MAY was acquired in 97% M/W solvent content after 8 h incubation at 17℃. The hig.est DFRIP of the extract was attained using.97% A/W solvent content after 16 h incubation at 35℃. The low cost of this process, on economic and environmental levels, could lead to interesting.applications on an industrial scale. It could be used to obtain bioactive phytochemicals from direct material or byproducts for either therapeutic or nutritional purposes.

关键词： Phenolic Compounds Monomeric Anthocyanins Antiradical Scaveng.ng Potential Extraction Optimization g.ape Time Solvent and Temperature

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Extraction of Total Phenolic Compounds, Flavonoids, Anthocyanins and Tannins from g.ape Byproducts by Response Surface Methodolog.. Influence of Solid-Liquid Ratio, Particle Size, Time, Temperature and Solvent Mixtures on the Optimization Process

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Food and nutrition Sciences 2014年第4期5卷 397-409页

作者： Hiba n. Rajha nada El Darra Zeina Hobaika nadia Boussetta Eug.ne Vorobiev richard g. maroun.n.colas louka Centre d’Analyses et de Recherche UR TVA Faculté des Sciences Université Saint-Joseph de Beyrouth Lebanon Centre d’Analyses et de Recherche UR TVA Faculté des Sciences Université Saint-Joseph de Beyrouth Lebanon UTC/ESCOM EA 4297 TIMR Département de Génie des Procédés Industriels Laboratoire Transformations Intégrées de la Matière Renouvelable Université de Technologie de Compiègne Centre de Recherche de Royallieu Compiègne France. UTC/ESCOM EA 4297 TIMR Département de Génie des Procédés Industriels Laboratoire Transformations Intégrées de la Matière Renouvelable Université de Technologie de Compiègne Centre de Recherche de Royallieu Compiègne France.

The current work concerns the optimization process of phenolic compounds solid liquid extraction from g.ape byproducts at hig. temperatures and short incubation times. The effect of five experimental parameters (solidliquid ratio, particle size, time, temperature and solvent mixture) mostly believed to affect the extraction process was undertaken. A first response surface methodolog. experimental desig. was used to optimize the solid-liquid ratio and milling.time parameters. A second desig. was used for the optimization of the quantitative and qualitative parameters. The quantitative parameters studied are: total phenolic compounds, flavonoid content, total monomeric anthocyanin composition and tannin concentration. The qualitative parameters analyzed are: antiradical activity and antioxidant capacity. The second desig. was based on the use of time, temperature and solvent mixture as optimization parameters. The assays were first conducted separately revealing.the best experimental conditions for the maximization of each response variable alone. A simultaneous response surface methodolog. of all the responses taken tog.ther was then conducted, showing.the optimal extraction conditions to be: 93 minutes at 94?C and in 66% ethanol/water solvent. The maximal response values obtained for each parameter are: Total Phenolic Compounds yield (5.5 g.g.E/100g.DM), Flavonoid Content (5.4 g.g.E/100g.DM), Total Monomeric Anthocyanin yield (70.3 mg.100g.DM), Tannin Concentration (12.3 g.L), Antiradical Activity (67.3%) and Total Antioxidant Capacity (393 mg.AE/L). All of the optimal values were acquired at 3 mL/g.solid-liquid ratio and 6.8 min milling.time. The obtained extracts could be used as natural bioactive compounds in several industrial applications.

关键词： Phenolic Compounds g.ape Byproducts Extraction Optimization Response Surface Methodolog. Antioxidant

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Multiple Response Optimization of Hig. Temperature, Low Time Aqueous Extraction Process of Phenolic Compounds from g.ape Byproducts

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Food and nutrition Sciences 2014年第4期5卷 351-360页

作者： Hiba n. Rajha nicolas louka nada El Darra Zeina Hobaika nadia Boussetta Eug.ne Vorobiev richard g. maroun Centre d’Analyses et de Recherche UR TVA Faculté des Sciences Université Saint-Joseph de Beyrouth Lebanon Centre d’Analyses et de Recherche UR TVA Faculté des Sciences Université Saint-Joseph de Beyrouth Lebanon UTC/ESCOM EA 4297 TIMR Département de Génie des Procédés Industriels Laboratoire Transformations Intégrées de la Matière Renouvelable Université de Technologie de Compiègne Centre de Recherche de Royallieu Compiègne France. UTC/ESCOM EA 4297 TIMR Département de Génie des Procédés Industriels Laboratoire Transformations Intégrées de la Matière Renouvelable Université de Technologie de Compiègne Centre de Recherche de Royallieu Compiègne France

In this study phenolic compounds extraction from g.ape byproducts was conducted using.pure water as a solvent. Hig. temperatures and low time incubation periods were used in the aim of reducing.the cost of the process and heig.tening.the phenolic compounds yield. Response surface methodolog. (RSM) was realized to study the effect of time and temperature on crushed and uncrushed g.ape pomace. The phenolic content was evaluated considering.the quantity (total phenolics (TPC), flavonoids (FC), total monomeric anthocyanins (TMA) and tannins (TC)), and quality (antiradical activity (AA) and antioxidant capacity (AC)) of the extracts. Hig. temperature low time extraction desig. used in this study was compared to the extraction process at moderate temperatures with relatively long.periods of time. This was proved to ameliorate the quantitative extraction of phenolic compounds from g.ape pomace without affecting.their bioactivity. Moreover, multiple response optimization showed the optimal extraction parameters to be 81?C and 140 minutes for the unmilled pomace samples, and 88?C and 5 minutes for the milled. TPC, FC, TMA, TC, AA and AC are almost the same for both optimums. Thus the possibility of replacing.the milling.process by the extraction time prolong.tion (for the unmilled pomace) of 135 minutes seems to be very plausible. HPLC analysis showed different quantity and diversity of extracted phenolics for the optimums. However this difference did not sig.ificantly affect the overall activity, showing.that PC in the different extracts act in complete synerg. all tog.ther leading.to important biolog.cal properties. The obtained results using.the extraction strateg. adopted in this work could lead to several industrial applications.

关键词： Phenolic Compounds g.ape Byproducts Optimization Aqueous Extraction Response Surface Methodolog.

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A Comparative Study on Antiradical and Antimicrobial Properties of Red g.apes Extracts Obtained from Different <i&g.;Vitis vinifera</i&g.;Varieties

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Food and nutrition Sciences 2012年第10期3卷 1420-1432页

作者： nada El Darra Joanna Tannous Paulette Bou Mouncef James Palg. Joseph Yag.i Eug.ne Vorobiev nicolas louka richard g. maroun Chateau KSARA S.A.L Ksara Békaa Valley Lebanon. Unité de Biochimie and Centre d’Analyse et de Recherche Faculté des Sciences Université Saint-Joseph de Beyrouth 1107 2050 Beirut Lebanon Université de Technolog.e de Compièg.e Département de Génie chimique Unité Transformations Intégrées de la Matière Renouvelable Centre de Recherche de Royallieu B.P. 20529-60205 Compiègne Cedex France

The present work is devoted to the study of antiradical and antimicrobial activities of phenolic compounds extracted from different g.apevine varieties g.own in the Bekaa plane-Lebanon. The amount of phenolic compounds in selected g.ape extracts was determined by the Folin-Ciocalteau method. Phenolic composition was specified by hig. performance liquid chromatog.aphy. Free radical scaveng.ng.activity was examined by using.the (2,2’-diphenyl-1-picrylhy-drazyl) DPPH assay. The potential antimicrobial activity was studied using.a new quantitative method developed during.this work. This activity was tested ag.inst several microbial pathog.ns, including.a g.am-positive strain (Listeria monocytog.nes), two g.am-neg.tive strains (Escherichia coli and Salmonella arizonae) and a fung.l strain (Candida albicans). According.to the results of the present screening.study, a g.eat variability in the composition of phenolic com-pounds in red g.ape extracts was detected. All phenolic compounds extracts, demonstrated important scaveng.ng.properties and antimicrobial effect ag.inst bacterial and fung.l strains. Yet, a different response deg.ee was noticed depending.on the tested microorg.nism and the phenolic composition of g.ape extracts. Antimicrobial activity was more effective ag.inst g.am-positive than g.am-neg.tive and yeast strains. Furthermore, our results hig.lig.ted a sig.ificant role of synerg.stic effect between various phenolic compounds in the free radical scaveng.ng.and antimicrobial activities.

关键词： Phenolic Compounds DPPH HPLC-DAD Antimicrobial Antiradical g.apevines and Synerg.stic Effect

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The Benefits of Patients’ Information reg.rding.Dietary Habits before Percutaneous Coronary Intervention: A 3- and 6-Month Follow-Up

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World Journal of Cardiovascular Diseases 2018年第6期8卷 307-318页

作者： Arg.riou g.org. Vasilopoulos g.org. Mavrog.anni g.org.a Toulia g.org.a louka Aikaterini louka Sofia Karag.annis Anastasios Lazos g.briel Marvaki Aikaterini Kadda Olg. Intensive Care Unit 1st Department of Respiratory Medicine National and Kapodistrian University of Athens “Sotiria” Gen-eral Chest Diseases Hospital Greece nursing.Department Technological Educational Institute of Athens Greece Cardiolog.cal Intensive Care Unit “Konstantopoulio” General Hospital of N. Ionias Greece g.aduates of nursing.Department Technological Educational Institute of Athens Greece Cardiolog.cal Intensive Care Unit Onassis Cardiac Surgery Centre Greece g.neral Hospital of Katerini Greece

Aim: To evaluate the benefits of counseling.intervention related to dietary habits chang.s on patients following.percutaneous coronary intervention (PCI). Materials and Methods: A randomized counseling.intervention study, with a 3- and 6-month follow-up was performed on 230 patients who underwent PCI. They were randomly allocated to the intervention (n = 93) or the control g.oup (n = 137). A 3- and 6-month telephone follow-up was performed for dietary habits evaluation. Data analysis was performed by using.the statistical packag. SPSS, ver. 20.?Results: Compared with control g.oup, intervention g.oup had hig.er prevalence of hypertension, history of diabetes and dyslipidemias and history of acute myocardial infraction, with no statistical difference. Moreover, patients in control g.oup were more likely to be ex-smokers (p = 0.01). Post hoc tests using.the Bonferroni correction revealed that mean TCHOL concentration while patients admitted to hospital differed statistically sig.ificantly between the time points of 3 and 6 months (209 ± 67 mg.dl vs 174 ± 34 mg.dl vs 176 ± 36 mg.dl), p= 0.005 and p = 0.042 respectively. However, there was no statistical sig.ificant difference between 3 months and 6 months measurements. Mean g.ucose concentration while patients admitted to hospital differed statistically sig.ificantly between the time points of 3 and 6 months (108 ± 40 mg.dl vs 95 ± 21 mg.dl vs 95 ± 23 mg.dl), p =0.009 and p = 0.012 respectively. However, there was no sig.ificant statistical difference between 3 months and 6 months measurements, (p = 1.000). Conclusion: A nurse-led prog.am reg.rding.dietary habits modifications on patients underg.ing.PCI should be performed along.with a long.term follow up after hospital discharg..

关键词： Dietary Habits Intervention MedDietScore Percutaneous Coronary Intervention Patient’s Information

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