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Specific interaction of hepatitis C virus glycoproteins with mannan binding lectin inhibits virus entry

Protein & Cell 2010年第7期1卷 664-674页

作者： Kristelle S.Brown Michael J.Keogh Ania M.Owsianka richard adair Arvind H.Patel James N.Arnold Jonathan K.Ball Robert B.Sim Alexander W.Tarr Timothy P.Hickling Institute of Infection Immunity and InflammationSchool of Molecular Medical SciencesThe University of NottinghamQueen’s Medical CentreNottinghamNG72UHUK The Medical Research Council-University of Glasgow Centre for Virus Research University of GlasgowChurch StreetGlasgowG115JRUK MRC Immunochemistry Unit Department of BiochemistryUniversity of OxfordOX13QUUK Present address:Vitrology Biotech 5 South AvenueClydebank Business ParkGlasgowG812LGUK Present address:Pfizer Technologies Ltd. Ramsgate RoadSandwichKentCT139NJUK

Mannan-binding lectin(MBL)is a soluble innate immune protein that binds to glycosylated *** acts as an opsonin and activates complement,contributing to the destruction and clearance of infecting *** C virus(HCV)encodes two envelope glycoproteins E1 and E2,expressed as non-covalent E1/E2 heterodimers in the viral envelope.E1 and E2 are potential ligands for *** we describe an analysis of the interaction between HCV and MBL using recombinant soluble E2 ectodomain fragment,the full-length E1/E2 heterodimer,expressed in vitro,and assess the effect of this interaction on virus entry.A binding assay using antibody capture of full length E1/E2 heterodimers was used to demonstrate calcium dependent,saturating binding of MBL to HCV *** with various saccharides further confirmed that the interaction was via the lectin domain of *** binds to E1/E2 representing a broad range of virus *** was shown to neutralize the entry into Huh-7 cells of HCV pseudoparticles(HCVpp)bearing E1/E2 from a wide range of *** were neutralized to varying *** was also shown to neutralize an authentic cell culture infectious virus,strain JFH-1(HCVcc).Furthermore,binding of MBL to E1/E2 was able to activate the complement system via MBL-associated serine protease *** conclusion,MBL interacts directly with HCV glycoproteins,which are present on the surface of the virion,resulting in neutralization of HCV particles.

关键词： hepatitis C virus neutralization mannose binding lectin chronic viral infection

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