检索结果-南通市图书馆

水稻种子特异表达基因OsEnS38的克隆与表达

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

湖南农业大学学报（自然科学版） 2018年第3期44卷 278-283页

作者：张焕仇忠凯阎新欧阳解秀李绍波王鑫南昌大学生命科学学院江西南昌330031 江西省分子生物学与基因工程重点实验室江西南昌330031

以粳稻中花11为材料,利用生物信息学技术,结合RT–PCR,克隆了水稻OsEnS38的c DNA序列;其编码的蛋白序列包含2个Cupin_1保守结构域,属于典型的Bicupin亚家族。启动子分析发现,OsEnS38启动子区域含有多种与胚乳特异表达相关的顺式作用元件... 详细信息

以粳稻中花11为材料,利用生物信息学技术,结合RT–PCR,克隆了水稻OsEnS38的c DNA序列;其编码的蛋白序列包含2个Cupin_1保守结构域,属于典型的Bicupin亚家族。启动子分析发现,OsEnS38启动子区域含有多种与胚乳特异表达相关的顺式作用元件;q RT–PCR和原位杂交结果证实,OsEnS38在开花后7~14 d的种子中特异表达,在第10天的胚乳中表达量最高,达29.73;杂交信号在第7、10天的胚乳中高表达;共表达分析显示,共表达基因参与水稻胚后发育和生殖发育调控,表明该基因在种子发育和储藏物质积累过程中发挥作用。

关键词：水稻种子特异表达基因基因克隆原位杂交共表达分析

Thermoelectric properties of (GeTe)_(1-x)[(Ag_(2)Te)_(0.4)(Sb_(2)Te_(3))_(0.6)]_(x) alloys

在线全文

学校读者我要写书评

暂无评论

Rare Metals 2022年第3期41卷 921-930页

作者： Hong-Xia Liu Xin-Yue Zhang Zhong-Lin Bu Wen Li yan-Zhong Pei School of Materials Science and Engineering Taiyuan University of Science and TechnologyTaiyuan 030024China Laboratory of Magnetic and Electric Functional Materials and the Applications The Key Laboratory of Shanxi ProvinceTaiyuan 030024China Interdisciplinary Materials Research Center School of Materials Science and EngineeringTongji UniversityShanghai 201804China

The(GeTe)_(x)(AgSbTe_(2))_(100-x)alloys(TAGS-x)have been demonstrated as a promising p-type candidate for thermoelectric applications,attracting numerous atten-tions on the advancements of thermoelectric *** of carrier concentration for optimizing ther-moelectric performance in TAGS can be achieved by varying the ratio of Ag to Sb,and the Ag/Sb ratio of~2/3 has been proven as the optimal ***,this work focuses on the systematic investigation on ther-moelectric properties of(GeTe)_(1-x)[(Ag_(2)Te)_(0.4)(Sb_(2)Te_(3))_(0.6)]_(x) *** crystal structure for the alloys transfers from rhombohedral to cubic at room temperature as x≥*** evolution of band parameter is estimated using a single parabolic band(SPB)model with acoustic phonon *** density of states effective mass increases with x increasing,which leads to an enhancement of Seebeck coefficient along with a reduction in Hall mobility due to the additional carrier scattering by point ***-while,the lattice thermal conductivity of lower than~0.7 W·m^(-1)·K^(-1) in the entire temperature range and the lowest one of only 0.45 W·m^(-1)·K^(-1) is achieved due to additional phonon scattering by point *** a result,a peak thermoelectric figure of merit(zT)of~1.80 and an average one of~1.37 in 300–800 K are realized in non-stoichiometric TAGS alloys here.

关键词： Thermoelectric Tellurium-antimonygermanium-silver(TAGS) Crystal structure Lattice thermal conductivity

Quantitative analysis of FRET assay in biology New developments in protein interaction affinity and protease kinetics determinations in the SUMOylation cascade

在线全文

学校读者我要写书评

暂无评论

Frontiers in Biology 2012年第1期7卷 57-64页

作者： yan LIU yang SONG Ling JIANG Jiayu LIA Department of Bioengineering Bourns College of Engineering University of California at Riverside 900 University Avenue Riverside CA92521 USA

Forster resonance energy transfer （FRET） techniques have been widely used in biological studies in vitro andin vivo and are powerful tools for elucidating protein interactions in many regulatory cascades. FRET occurs between oscillating dipoles of two fluorophores with overlapping emission and excitation wavelengths and is dependent on the spectroscopic and geometric properties of the donor-acceptor pair. Various efforts have been made to develop quantitative FRET methods to accurately determine the interaction affinity and kinetics parameters. SUMOylation is an important post-translational protein modification with key roles in multiple biological processes. Conjugating SUMO to substrates requires an enzymatic cascade. Sentrin/SUMO-specific proteases （SENP） act as endopeptidases to process the pre-SUMO or an isopeptidase to deconjugate SUMO from its substrate. Here we also summarize recent developments of theoretical and experimental procedures for determining the protein interaction dissociation constant, Kd, and protease kinetics parameters, kcat and kin, in the SUMOylation pathway. The general principles of these quantitative FRET-based measurements can be applied to other protein interactions and proteases.

关键词： quantitative FRET analysis protein affinity determination kinetics analysis

A Potential Anti-metastatic Chinese Herb Andrographis paniculata—Its In Vitro Activities in Esophageal Cancer Cells and Endothelial Cells

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A Potential Anti-metastatic Chinese Herb Andrographis panicu...

Cystic meconium peritonitis with jejunoileal atresia:Is it associated with unfavorable outcome?

第十四届全国中药和天然药物学术研讨会

作者： Grace Gar-Lee Yue Julia kin-Ming Lee Kar-Man Chan Kwok-Pui Fung Philip Wai-yan Chiu Clara Bik-San Lau The Chinese University of Hong Kong

＜正＞Esophageal cancer is associated with high postoperative recurrence and systemic *** adjuvant chemotherapy and concurrent radiation have shown favourable efficacies,searching for alternative treatments for metast...

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

World Journal of Clinical Pediatrics 2017年第1期6卷 40-44页

作者： kin Wai Edwin Chan Kim Hung Lee Hei Yi Vicky Wong Siu yan Bess Tsui Yuen Shan Wong Kit Yi Kristine Pang Jennifer Wai Cheung Mou Yuk Him Tam Division of Paediatric Surgery and Paediatric Urology Department of Surgerythe Prince of Wales Hospitalthe Chinese University of Hong Kong

AIM To compare the outcome between patients with jejunoileal atresia(JIA) associated with cystic meconium peritonitis(CMP) and patients with isolated JIA(JIA without CMP).METHODS A retrospective study was conducted for all neonates with JIA operated in our institute from January 2005 to January *** including the gestation age,sex,birth weight,age at operation,the presence of associated syndrome was *** outcome including the type of operation performed,operative time,the need for reoperation and mortality were *** demographics and the outcome between the 2 groups were *** During the study period,53 neonates had JIA underwent operation in our *** neonates(32%) were associated with *** was no statistical difference on the demographics in the two *** with CMP had earlier operation than patients with isolated JIA(mean 1.4 d vs 3 d,P = 0.038).Primaryanastomosis was performed in 16 patients(94%) with CMP and 30 patients(83%) with isolated JIA(P = 0.269).Patients with CMP had longer operation(mean 190 min vs 154 min,P = 0.004).There were no statistical difference the need for reoperation(3 vs 6,P = 0.606) and mortality(2 vs 1,P = 0.269) between the two *** Primary intestinal anastomosis can be performed in94% of patients with JIA associated with *** patients with CMP had longer operative time,the mortality and reoperation rates were low and were comparable to patients with isolated JIA.

关键词： Meconium Peritonitis Atresia Jejunoileal

Using proteomic approach to discover the treatment mechanism of（Z）3,4,5,4’-trans-tetramethoxystilbene for inhibition of gefitinib-resistant non-small cell lung cancer

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Using proteomic approach to discover the treatment mechanism...

甲藻(Dinophyta)凯伦藻科(Kareniaceae)的分类学研究与展望

第21届全国色谱学术报告会及仪器展览会

作者： Xing-Xing Fan Xiao-Jun Yao So Wei Xu Vincent Kam-Wai Wong Jian-Xing He Jian Ding Min Huang Jun Huang Da-Kai Xiao Ze-Bo Jiang yan-Ling Zhou richard kin-Ting Kam Liang Liu Elaine Lai-Han Leung State Key Laboratory of Quality Research in Chinese Medicine/Macau Institute For Applied Research in Medicine and Health Macau University of Science and Technology Guangzhou Institute of Respiratory Disease State Key Laboratory of Respiratory DiseaseThe 1st Affiliated Hospital of Guangzhou Medical College State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academic of Sciences Department Chemical Pathology Faculty of Medicinethe Chinese University of Hong Kong

Calcium is a second messenger which is required for regulation of many cellular processes. However,excessive elevation or prolonged activation of calcium signaling would lead to cell death. As such,selectively regulating calcium signaling could be an alternative approach for anti-cancer therapy. Recently,we have identified an effective analogue of resveratrol,（Z）3,4,5,4’-trans-tetramethoxystilbene（TMS） which selectively elevated the intracellular calcium level in gefitinib-resistant（G-R） non-small-cell lung cancer（NSCLC） cells. TMS exhibited significantly inhibitory effect on G-R NSCLC cells, but not other NSCLC cells and normal lung epithelial cells. The phosphorylation and activation of EGFR was inhibited by TMS in G-R cells. TMS induced caspase-independent apoptosis and autophagy by directly binding to SERCA and causing endoplasmic reticulum（ER） stress and AMPK activation. Using i TRAQ proteomics analysis,we have further confirmed that mTOR pathway, which is the downstream of AMPK, was significantly suppressed by TMS. JNK, the cross-linker of ER stress and mTOR pathway was significantly activated by TMS. In addition, the inhibition of JNK activation can partially block the effect of TMS. Taken together,TMS showed promising anti-cancer activity by mediating calcium signaling pathway and inducing apoptosis as well as autophagy in G-R NSCLC cells, providing strategy in designing multi-targeting drug for treating G-R patients.

关键词： (Z)3,4,5,4’-trans-tetramethoxystilbene(TMS) non-small-cell lung cancer gefitinib resistance mTOR and autophagy

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

海洋与湖沼 2017年第4期48卷 786-797页

作者：王建艳何建宗齐雨藻吕颂辉杨静北京自然博物馆北京100050 香港公开大学香港九龙999077 暨南大学广州510632

凯伦藻科(Kareniaceae)是甲藻门中较新建立的一个科。凯伦藻科种类广泛分布于全球海域,目前已报道3属30个种。由于该科大部分种类频发藻华,严重威胁近海生态安全和水产养殖,因而备受关注。自20世纪90年代起,我国沿岸也逐渐成为凯伦藻藻... 详细信息

凯伦藻科(Kareniaceae)是甲藻门中较新建立的一个科。凯伦藻科种类广泛分布于全球海域,目前已报道3属30个种。由于该科大部分种类频发藻华,严重威胁近海生态安全和水产养殖,因而备受关注。自20世纪90年代起,我国沿岸也逐渐成为凯伦藻藻华的重灾区,凯伦藻曾引发我国最严重的几次藻华事件。本文对本科种类分类学特征进行详细描述,翻译其中文名,编制属、种分类检索表,以期为我国凯伦藻生物多样性研究以及凯伦藻藻华防治提供基础而必要的种源信息。

关键词：凯伦藻科凯伦藻属卡尔藻属塔卡藻属分类学

Apatinib(YN968D 1) enhances the efficiency of conventional chemotherapeutical drugs by inhibiting P-glycoprotein function in leukemia cells

在线全文

学校读者我要写书评

暂无评论

Apatinib(YN968D 1) enhances the efficiency of conventional c...

上笼应激对不同品种蛋鸭血清生化指标和抗氧化能力的影响

2011医学科学前沿论坛第十二届全国肿瘤药理与化疗学术会议

作者： Shu Liang,Xu Zhang,Jie-hua He,Xing-Gui Chen,Yong-ju Liang,Fang Wang, yan-jun Mi,Kenneth kin Wah To,Xiu-zhen Tong,Li-wu Fu

Background:P-glycoprotein(P-gp,ABCB1) overexpression and enrichment of stem-like cells have been shown to correlate with poor prognosis in leukemia patients. Methodology/Principal Findings:We investigate the effect of apatinib,an oral small-molecule multi-targeted tyrosine kinase inhibitor(TKI),on the efficiency of conventional anticancer drugs in leukemia side population(SP) cells and ABCB1-,ABCC1/MRP1-,ABCC4/MRP4-,ABCC10/MRP7- and lung resistance protein(LRP)-overexpression cancer *** results show that apatinib significantly enhanced the cytotoxicity of conventional chemotherapeutic drugs in SP

关键词： multidrug resistance ATP-binding cassette transporters P-glycoprotein apatinib leukemia stem-like cells ABCB1 ABCG2 Doxorubicin side population leukemia cells

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

中国家禽 2024年第4期46卷 73-79页

作者：刘锦玉芦燕顾天天田勇陈黎曾涛许文武卢立志吴旭福建农林大学动物科学学院(蜂学学院) 福建福州350002 浙江省农业科学院畜牧兽医研究所浙江杭州310021 诸暨市人民政府暨南街道办事处浙江绍兴311800

为探讨品种和饲养模式对鸭上笼应激的血清生化指标的影响,研究以缙云麻鸭和金定鸭为研究对象,分别采用平养和笼养的养殖方式探究上笼应激对其血清生化指标和抗氧化能力的影响。结果显示:品种和饲养模式的交互作用对抗氧化能力指标超氧... 详细信息

为探讨品种和饲养模式对鸭上笼应激的血清生化指标的影响,研究以缙云麻鸭和金定鸭为研究对象,分别采用平养和笼养的养殖方式探究上笼应激对其血清生化指标和抗氧化能力的影响。结果显示:品种和饲养模式的交互作用对抗氧化能力指标超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC)和血清细胞因子白细胞介素-6(IL-6)有显著(P<0.05)或极显著影响(P<0.01);饲养模式对抗氧化能力指标过氧化氢酶(CAT)、丙二醛(MDA)和肝功能指标白蛋白(ALB)、血清细胞因子免疫球蛋白M(IgM)、免疫球蛋白G(IgG)、白细胞介素-1β(IL-1β)、白细胞介素-2(IL-2)和白细胞介素-6(IL-6)有显著(P<0.05)或极显著影响(P<0.01)。其中平养模式丙二醛、白蛋白、免疫球蛋白G、白细胞介素-1β、白细胞介素-2和白细胞介素-6含量显著(P<0.05)或极显著(P<0.01)低于笼养模式,其他指标则显著高于笼养模式(P<0.01);品种对抗氧化能力指标超氧化物歧化酶有显著影响,金定鸭极显著高于缙云麻鸭(P<0.01)。研究表明上笼应激对缙云麻鸭和金定鸭肝脏、心脏和肾功能产生的应激损伤不显著,但对其抗氧化能力损伤较为明显。

关键词：上笼应激缙云麻鸭金定鸭生化指标抗氧化能力