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Prevascularized spongy-like hydrogels maintain their angiogenic potential after prolonged hypothermic storage

Bioactive Materials 2024年第7期37卷 253-268页

作者： Sara Freitas-Ribeiro Helena Moreira Lucília P.da Silva Jennifer Noro Belem Sampaio-Marques Paula Ludovico Mariana Jarnalo ricardo horta Alexandra P.Marques Rui L.Reis Rogerio P.Pirraco B’s Research Group I3Bs-Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAveParkParque de Ciencia e TecnologiaZona Industrial da Gandra4805-017BarcoGuimaraesPortugal ICVS/3B’s-PT Government Associate Laboratory Braga/GuimaraesPortugal Life and Health Sciences Research Institute(ICVS) School of MedicineUniversity of MinhoBragaPortugal Department of Plastic and Reconstructive Surgery and Burn UnityCentro Hospitalar de Sao JoaoPortoPortugal Faculty of Medicine-University of Porto Portugal

The chronic shortage of organs and tissues for transplantation represents a dramatic burden on healthcare systems *** engineering offers a potential solution to address these shortages,but several challenges remain,with prevascularization being a critical factor for in vivo survival and integration of tissue engineering ***,a different challenge hindering the clinical implementation of such products,regards their efficient preservation from the fabrication site to the *** has emerged as a potential solution for this issue due to its milder effects on biologic systems in comparison with other cold preservation *** impact on prevascularization,however,has not been well *** this work,3D prevascularized constructs were fabricated using adipose-derived stromal vascular fraction cells and preserved at 4◦C using Hypothermosol or basal culture media(α-MEM).Hypothermosol efficiently preserved the structural and cellular integrity of prevascular networks as compared to constructs before *** contrast,the use ofα-MEM led to a clear reduction in prevascular structures,with concurrent induction of high levels of apoptosis and autophagy at the cellular *** vivo evaluation using a chorioallantoic membrane model demonstrated that,in opposition toα-MEM,Hypothermosol preservation retained the angiogenic potential of constructs before preservation by recruiting a similar number of blood vessels from the host and presenting similar integration with host *** results emphasize the need of studying the impact of preservation techniques on key properties of tissue engineering constructs such as prevascularization,in order to validate and streamline their clinical application.

关键词： Tissue engineering Prevascularization Hypothermic preservation Clinical translation

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A compact underwater stereo vision system for measuring fish

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Aquaculture and Fisheries 2024年第6期9卷 1000-1006页

作者： Cesar Silva ricardo Aires Flavio Rodrigues Fishmetrics LdaHorta9900-146Portugal

contributes for decisions at several production ***,taking length measurements is a cumbersome task that,when applied to living individuals,can induce considerable stress,increasing the risk of damage or hindering their *** vision is one of the most used non-contact tools to overcome this issue,being fast,consistent and ***,its use in aquatic environments is limited by the high cost,the difficulty of calibrating the system in underwater conditions and the complexity of *** paper proposes a low-cost easy-to-use vision system that can take measurements on live fish in aquatic conditions,without the need for a special calibration or a demanding in-water calibration *** present work implemented a compact stereo vision system and developed a method that estimates the correct length of fish,based on the variation of the angle of incidence of the light rays in the *** some structural conditions such as a short baseline,the system is able to measure fish with an error of less than 1%.The short baseline allows to have a compact system and reduces the effect of water refraction on the 3D reconstruction.A set of experiments were performed with real fish,working robustly for a set of orientations of the fish(even when the caudal fin and snout are on different distances to the cameras).

关键词： Underwater stereo vision Fish measuring Fish length Aquaculture technology

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