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Proteomic profile of seminal plasma in adolescents and adults with treated and untreated varicocele

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Asian Journal of Andrology 2016年第2期18卷 194-201,J0003页

作者： Mariana camargo Paula Intasqui ricardo Pimenta Bertolla Department of Surgery Division of Urology Human Reproduction Section Sao Paulo Federal University R. Embau 231 04039-060 Sao Paulo Brazil Sao Paulo Hospital Sao Paulo Brazil.

Varicocele, the most important treatable cause of male infertility, is present in 15% of adult males, 35% of men with primary infertility, and 80% of men with secondary infertility. On the other hand, 80% of these men will not present infertility. Therefore, there is a need to differentiate a varicocele that is exerting a deleterious effect that is treatable from a ＂silent＂ varicocele. Despite the growing evidence of the cellular effects of varicocele, its underlying molecular mechanisms are still eluding. Proteomics has become a promising area to determine the reproductive biology of semen as well as to improve diagnosis of male infertility. This review aims to discuss the state-of-art in seminal plasma proteomics in patients with varicocele to discuss the challenges in undertaking these studies, as well as the future outlook derived from the growing body of evidence on the seminal proteome.

关键词： male infertility proteomics seminal plasma varicocele varicocelectomy

Tides and Wind-Driven Circulation in the Tropical and Southern Atlantic Ocean:The BRAZCOAST System

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Atmospheric and Oceanic Science Letters 2014年第5期7卷 476-480页

作者： ricardo de camargo Joseph HARARI Department of Atmospheric Sciences University of Sao Paulo Sao Paulo Brazil Department of Physical Chemical and Geological Oceanography University of Sao Paulo Sao Paulo Brazil

The Brazilian coast is characterized by different tidal regimes and distinct meteorological influences. The northern part has larger tidal amplitudes and is permanently affected by trade winds and tropical disturbances; the southern portion has smaller tidal amplitudes and is frequently influenced by extratropical cyclone activity. Besides these aspects, many features regarding current structure and behavior are also present, such as the equatorial system of currents, the subtropical gyre and the corresponding western boundary currents, and the Brazil-Malvinas confluence region. Within this context, efforts were made to develop the BRAZCOAST system, capable of describing the processes that determine the oceanic circulation from large to coastal scales. A customized version of the Princeton Ocean Model(POM) was implemented in a basin-scale domain covering the whole of the tropical and southern Atlantic Ocean, with 0.5° spatial resolution, as well as three nested grids with(1/12)° resolution covering the different parts of the Brazilian shelf, in a one-way procedure. POM was modified to include tidal potential generator terms and a partially-clamped boundary condition for tidal elevations. The coarse grid captured large-scale features, while the nested grids detailed local circulations affected by bathymetry and coastal restrictions. An interesting aspect at the coarse grid level was the relevance of the Weddell Sea to the location of the tidal amphidromic systems.

关键词： tides wind-driven circulation tropical Atlan-tic South Atlantic Princeton Ocean Model

Zeta potential measurement on lithium lanthanum titanate nanoceramics

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Particuology 2016年第1期14卷 69-72页

作者： Carlos ricardo Milian Pila Eduardo perez Cappe Yodalgis Mosqueda Laffita Oswaldo Luiz Alves Institute of Materials Science and Technology (IMRE) Havana University Havana CP 10400 Cuba Solid state Chemistry Laboratory Institute of Chemistry Campinas University Campinas CP 13083-970 SP Brazil

The zeta potential, isoelectric point, and agglomeration of ***3 （LLTO） nanoparticles dispersed in aqueous media at different ionic strengths have been studied. The zeta potential was determined from electrophoretic mobility measurements, according to Smoluchowski＇s equation, for Li0.5La0.5TiO3 suspen- sions in NaCl and KCI electrolytes with ionic strengths of 1, 10, and 100 mmol/dm3. The isoelectric point （IEP）, zeta potential （ζ）, and the agglomeration were shown to strongly depend on the ionic strength of the Li0.5La0.5TiO3 aqueous colloidal suspension in both NaCI and KCI electrolytes, which allows the deter- mination of the effects of environmental conditions for ***3 manipulation in aqueous colloidal systems. The suspensions of Li0.5La0.5TiO3 nanoparticles reach the IEP in the pH range 0f3-5. The （ of Li0.5La0.5TiO3 nanoparticles varied from positive to negative values with a pH increase, which allows for the control of the surface charge depending on the purpose. The pH range of 7-g and an ionic strength ≤1 mmol/dm3 are recommended as the most suitable conditions for both the LLTO colloidal shaping techniques application and the LLTO-based nanocomposite formation.

关键词： Titanate Nanoparticles Zeta potential Colloidal suspension

The nest architecture of the ant, Pheidole oxyops Forel, 1908 （Hymenoptera： Formicidae）

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Insect Science 2007年第5期14卷 437-442页

作者： LUIZ C. FORTI ROBERTO S. camargo ricardo T. FUJIHARA JULIANE F. S. LOPES Laboratorio de Insetos Sociais-Praga Departamento de Producao Vegetal - Setor Defesa Fitossanitaria FCA/UNESP Botucatu SP Comportamento e Ecologia Animal Instituto de Ciencias Biologicas Universidade Federal de Juiz de Fora Campus Universitario Martelos Juiz de Fora MG Brazil

Pheidole oxyops builds subterranean nests, with an external architecture that is distinctive and easily recognizable by its wide and specific entrance hole, measuring up to 12.2 cm in diameter, denoting a pitfall-trap. In order to study the nests＇ internal architecture, seven nests were excavated; four were identified with neutral talc, while the others were cast in cement and then excavated. Measurements were made in order to gain a better understand- ing of their structures, and a photographic documentation was obtained as well. The excavations revealed that the nests are perpendicular relative to the ground, beginning with a cylindrical channel with a mean length of 13.5 cm, containing irregular formations, and whose diameter becomes progressively narrower until the first chamber is formed. As the channel continues, dish-like chambers appear, interconnected by channels that become progressively narrower and longer, while the chambers are arranged at greater distances from each other as nest depth increases. Both channels and chambers are located on the vertical projection of the entrance hole. Nests may reach a depth of up to 5.09 m, with a number of chambers ranging between 4 and 14.

关键词： chambers nest architecture Pheidole pitfall-trap

Interpolation of Vector Measures

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Acta Mathematica Sinica,English Series 2011年第1期27卷 119-134页

作者： ricardo del CAMPO Antonio FERNANDEZ Fernando MAYORAL Francisco NARANJO Enrique A. SANCHEZ-perez Dpto. Matemdtica Aplicada I Universidad de SevillaEUITA Ctra. de Utrera Kin. 1 41013 Sevilla Spain Dpto. Matemdtica Aplicada II Universidad de Sevilla Escuela Tdcnica Superior de Ingenieros Camino de los Descubrimientos s/n 41092 Sevilla Spain Instituto Universitario de Matemdtica Pura y Aplicada Universidad Politdcnica de Valencia Camino de Vera s/n 46022 Valencia Spain

Let （Ω, ∑） be a measurable space and mo ： E→ Xo and m1 ： E → X1 be positive vector measures with values in the Banach KSthe function spaces Xo and X1. If 0 〈 a 〈 1, we define a X01-ax1a new vector measure [m0, m]a with values in the Calderdn lattice interpolation space and we analyze the space of integrable functions with respect to measure [m0, m1]a in order to prove suitable extensions of the classical Stein Weiss formulas that hold for the complex interpolation of LP-spaces. Since each p-convex order continuous Kothe function space with weak order unit can be represented as a space of p-integrable functions with respect to a vector measure, we provide in this way a technique to obtain representations of the corresponding complex interpolation spaces. As applications, we provide a Riesz-Thorin theorem for spaces of p-integrable functions with respect to vector measures and a formula for representing the interpolation of the injective tensor product of such spaces.

关键词： Interpolation Banach function space vector measure

Design and Implementation of a Rehabilitation Upper-limb Exoskeleton Robot Controlled by Cognitive and Physical Interfaces

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Journal of Bionic Engineering 2022年第5期19卷 1374-1391页

作者： Arturo Gonzalez‑Mendoza Ivett Quinones‑Uriostegui Sergio Salazar‑Cruz Alberto‑Isaac perez‑Sanpablo ricardo Lopez‑Gutierrez Rogelio Lozano LAFMIA UMI Center for Research and AdvancedStudies of National Polytechnic InstituteAv.Instituto Politecnico Nacional No.250807360 Mexico CityMexico Motion Analysis Lab National Institute of Rehabilitation Luis Guillermo Ibarra IbarraCalz.Mexico Xochimilco No.28914389 Mexico CityMexico CONACYT-CINVESTAV Av.Instituto Politecnico Nacional 250807360 Mexico CityMexico UTC-CNRS UMR Sorbonne UniversitesUTC-CNRS UMR7253 HeudiasycCompiegneFrance

This paper presents an upper limb exoskeleton that allows cognitive(through electromyography signals)and physical user interaction(through load cells sensors)for passive and active exercises that can activate neuroplasticity in the rehabilitation process of people who suffer from a neurological *** the exoskeleton to be easily accepted by patients who suffer from a neurological injury,we used the ISO9241-210:2010 as a methodology design *** the first steps of the design process,design requirements were collected from previous usability tests and ***,as a second step,a technological solution is proposed,and as a third step,the system was evaluated through performance and user *** part of the technological solution and to allow patient participation during the rehabilitation process,we have proposed a hybrid admittance control whose input is load cell or electromyography *** hybrid admittance control is intended for active therapy exercises,is easily implemented,and does not need musculoskeletal modeling to ***,electromyography signals classification models and features were evaluated to identify the best settings for the cognitive human–robot interaction.

关键词： Human Robot interaction Hybrid admittance control Surface electromyography Upper-limb exoskeletal robot

Fatty acid oxidation and autophagy promote endoxifen resistance and counter the effect of AKT inhibition in ER-positive breast cancer cells

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Journal of Molecular Cell Biology 2021年第6期13卷 433-444页

作者： Lei Duan Sarah Calhoun Daeun Shim ricardo E.perez Lothar A.Blatter Carl G.Maki Department of Cell and Molecular Medicine Rush University Medical CenterChicagoIL 60612USA Department of Molecular Biophysics and Physiology Rush University Medical CenterChicagoIL 60612USA

Tamoxifen(TAM)is the first-line endocrine therapy for estrogen receptor-positive(ER+)breast cancer(BC).However,acquired resistance occurs in∼50%***,although the PI3K/AKT/mTOR pathway is a viable target for treatment of endocrine therapy-refractory patients,complex signaling feedback loops exist,which can counter the effectiveness of inhibitors of this ***,we analyzed signaling pathways and metabolism in ER+MCF7 BC cell line and their TAM-resistant derivatives that are co-resistant to endoxifen using immunoblotting,quantitative polymerase chain reaction,and the Agilent Seahorse XF *** found that activation of AKT and the energy-sensing kinase AMPK was increased in TAM and endoxifen-resistant ***,ERRα/PGC-1βand their target genes MCAD and CPT-1 were increased and regulated by AMPK,which coincided with increased fatty acid oxidation(FAO)and autophagy in TAM-resistant *** of AKT feedback-activates AMPK and ERRα/PGC-1β-MCAD/CPT-1 with a consequent increase in FAO and autophagy that counters the therapeutic effect of endoxifen and AKT ***,our results indicate increased activation of AKT and AMPK with metabolic reprogramming and increased autophagy in TAM-resistant *** inhibition of AKT and FAO/autophagy is necessary to fully sensitize resistant cells to endoxifen.

关键词： endoxifen fatty acid oxidation autophagy AMPK AKT

p53 promotes AKT and SP1-dependent metabolism through the pentose phosphate pathway that inhibits apoptosis in response to Nutlin-3a

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Journal of Molecular Cell Biology 2018年第4期10卷 331-340页

作者： Text Lei Duan ricardo E. perez Ling Chen Lothar A. Blatter Carl G. Maki Department of Cell & Molecular Medicine Rush University Medical Center Chicago IL 60612 USA Department of Laboratory Medicine Shiyan Taihe Hospital College of Biomedical Engineering Hubei University of Medicine Shiyan 442000 China Department of Physiology & Biophysics Rush University Medical Center Chicago IL 60612 USA

Nutlin-3a is a MDM2 antagonist and preclinical drug that activates p53. Cells with MDM2 gene amplification are especially prone to Nutlin-3a-induced apoptosis, though the basis for this is unclear. Glucose metabolism can inhibit apoptosis in response to Nutlin-3a through mechanisms that are incompletely understood. Glucose metabolism through the pentose phosphate pathway （PPP） produces NADPH that can protect cells from potentially lethal reactive oxygen species （ROS）. We compared apoptosis and glucose metabolism in cancer cells with and without MDM2 gene amplification treated with Nutlin-3a. Apoptosis in MDM2-amplified cells was associated with a reduction in glycolysis and the PPP, reduced NADPH, increased ROS, and depletion of the transcription factor SP1, which normally promotes PPP gene expression. In contrast, glycolysis and the PPP were maintained or increased in MDM2 non-amplified cells treated with Nutlin-3a. This was dependent on p53-mediated AKT activation and was associated with maintenance of SP1 and continued expression of PPP genes. Knockdown or inhibition of AKT, SP1, or the PPP sensitized MDM2-non-amplified cells to apoptosis. The data indicate that p53 promotes AKT and SP1-dependent activation of the PPP that protects cells from Nutlin-3a-induced apoptosis. These findings provide insight into how glucose metabolism reduces Nutlin-3a-induced apoptosis, and also provide a mechanism for the heightened sensitivity of MDM2-amplified cells to apoptosis in response to Nutlin-3a.

关键词： Nutlin p53 MDM2 SP1 glycolysis pentose phosphate pathway

Astrocytes and retrograde degeneration of nigrostriatal dopaminergic neurons in Parkinson’s disease:removing axonal debris

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Translational Neurodegeneration 2021年第4期10卷 554-572页

作者： Ingrid Morales ricardo Puertas-Avendaño Alberto Sanchez Adrian perez-Barreto Clara Rodriguez-Sabate Manuel Rodriguez Laboratory of Neurobiology and Experimental Neurology Department of Basic Medical SciencesFaculty of MedicineLa Laguna UniversityLa LagunaTenerifeCanary IslandsSpain Center for Networked Biomedical Research on Neurodegenerative Diseases(CIBERNED) MadridSpain

Objective:The dopaminergic nigrostriatal neurons(DA cells)in healthy people present a slow degeneration with aging,which produces cellular debris throughout *** 2%-5%of people present rapid cell degeneration of more than 50%of DA cells,which produces Parkinson's disease(PD).Neuroinflammation accelerates the cell degeneration and may be critical for the transition between the slow physiological and the rapid pathological degeneration of DA cells,particularly when it activates microglial cells of the medial forebrain bundle near dopaminergic *** synaptic debris produced by DA cell degeneration may trigger the parkinsonian neuroinflammation,this study investigated the removal of axonal debris produced by retrograde degeneration of DA cells,paying particular attention to the relative roles of astrocytes and ***:Rats and mice were injected in the lateral ventricles with 6-hydroxydopamine,inducing a degeneration of dopaminergic synapses in the striatum which was not accompanied by non-selective tissue damage,microgliosis or *** possible retrograde degeneration of dopaminergic axons,and the production and metabolization of DA-cell debris were studied with immunohistochemical methods and analyzed in confocal and electron microscopy ***:The selective degeneration of dopaminergic synapses in the striatum was followed by a retrograde degeneration of dopaminergic axons whose debris was found within spheroids of the medial forebrain *** spheroids retained mitochondria and most(e.g.,tyrosine hydroxylase,the dopamine transporter protein,and amyloid precursor protein)but not all(e.g.,α-synuclein)proteins of the degenerating dopaminergic *** showed initial(autophagosomes)but not late(lysosomes)components of autophagy(incomplete autophagy).These spheroids were penetrated by astrocytic processes of the medial forebrain bundle,which provided the lysosomes needed to continue the degradation of dopaminergic debri

关键词： Astrocyte Phagocytosis Parkinson's disease Dying-back degeneration Medial forebrain bundle Spheroid