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Molecular detection of Leishmania species in human and animals from cutaneous leishmaniasis endemic areas of Waziristan, Khyber Pakhtunkhwa, Pakistan

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Asian Pacific Journal of Tropical Medicine 2018年第8期11卷 495-500页

作者： Mubashir Hussain Shahzad Munir Abdullah Jalal Taj Ali khan Niaz Muhammad Bahar ullah Khattak Abdullah khan Irfan Ahmed Zulqarnain Baloch Nawaz Haider Bashir Muhammad Ameen Jamal Kashif Rahim Humaira Mazhar Maira riaz Noha Watany Vector Borne Diseases Lab Department of Microbiology Kohat University of Science and Technology Kohat Faculty of Plant Protection Yunnan Agricultural University Institute of Biotechnology and Genetic Engineering The University of AgriculturePeshawar Pakistan Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Yunnan Agricultural University College of Veterinary Medicine South China Agricultural University Department of Animal Breeding Genetics and Reproduction Yunnan Agricultural University Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology Institute of Biochemistry and Biotechnology College of Life Sciences Beijing Normal University Vector Biology Research Lab US Naval Medical Research Unit 3

Objectives: To detect Leishmania species in human patients, animal reservoirs and Phlebotomus sandflies in Waziristan, Pakistan. Methods: Tissue smears and aspirates from 448 cutaneous leishmaniasis(CL) suspected patients were analyzed. To sort out role of the reservoir hosts, skin scrapings, spleen and liver samples from 104 rodents were collected. Furthermore, buffy coat samples were obtained from 60 domestic animals. Sandflies were also trapped. All human, animals and sandfly samples were tested by microscopy, kinetoplastic PCR and internal transcribed spacer 1(ITS1) PCR followed by restriction fragment length polymorphism for detection of Leishmania species. Results: An overall prevalence of 3.83% and 5.21% through microscopy and ITS1 PCR respectively was found. However, the statistically non-significant correlation was found between area, gender, and number of lesions. The presence of rodents, sandflies, domestic animals and internally displaced people increased the risk of CL. Using ITS1-PCR-RFLP, Leishmania tropica(L. tropica) was confirmed in 106 samples while 25 of the isolates were diagnosed as Leishmania major(L. major). Similarly, 3/104 rodents were positive for L. major and 14 pools of DNA samples containing Phlebotomus sergenti sandflies were positive for L. tropica. None of samples from domestic animals were positive for leishmaniasis. Conclusions: In the present study, L. tropica and L. major are found to be the main causative agents of CL in study area. Movement of internally displaced people from CL endemic areas presents a risk for nearby CL free areas. To the best of our knowledge, we report for the first time L. major infection in rodents(Rattus rattus) and L. tropica in Phlebotomus sergenti sandflies trapped in Waziristan, Pakistan.

关键词： Leishmania major Rodents Cutaneous leishmaniasis Domestic animals ITS 1 PCR Waziristan

Regolith thickness modeling using a GIS approach for landslide distribution analysis, NW Himalayas

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Journal of Mountain Science 2018年第11期15卷 2466-2479页

作者： Muhammad BASHARAT Masood QASIM Muhammad SHAFIQUE Nasir HAMEED Muhammad Tayyib riaz Muhammad Rustam khan Institute of Geology University of Azad Jammu and Kashmir National Center of Excellence in Geology University of Peshawar Land Use Planning and Development Department Azad Jammu and Kashmir

Regolith thickness is considered as a contributing factor for the occurrence of ***, mostly it is ignored because of complex nature and as it requires more time and resources for investigation. This study aimed to appraise the role of regolith thickness on landslide distribution in the Muzaffarabad and surrounding areas, NW *** this purpose regolith thickness samples were evenly collected from all the lithological units at representative sites within different slope and elevation classes in the field. Topographic attributes(slope, aspect, drainage, Topographic Wetness Index,elevation and curvature) were derived from the Digital Elevation Model(DEM)(12.5 m resolution).Arc GIS Model Builder was used to develop the regolith thickness model. Stepwise regression technique was used to explore the spatial variation of regolith thickness using topographic attributes and lithological units. The derived model explains about 88% regolith thickness variation. The model was validated and shows good agreement(70%) between observed and predicted values. Subsequently, the derived regolith model was used to understand the relationship between regolith thickness and landslide distribution. The analysis shows that most of the landslides were located within 1-5 m regolith thickness. However, landslide concentration is highest within 5-10 m regolith thickness, which shows that regolith thickness played a significant role for the occurrence of landslide in the studied area.

关键词：厚度变化喜马拉雅山脉 GIS 表土山崩分发模特儿土模型

Analysis and Power Quality Improvement in Hybrid Distributed Generation System with Utilization of Unified Power Quality Conditioner

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Computer Modeling in Engineering & Sciences 2023年第2期134卷 1105-1136页

作者： Noor Zanib Munira Batool Saleem riaz Farkhanda Afzal Sufian Munawar Ibtisam Daqqa Najma Saleem Department of Electrical Engineering University of Engineering and TechnologyTaxila47050Pakistan School of Automation Northwestern Polytechnical University170072Xi’anChina National University Science&Technology MCSIslamabad44000Pakistan Department of Quantitative Methods College of Business AdministrationImam Abdulrahman Bin Faisal UniversityDammam34212Saudi Arabia Department of Mathematics and Natural Sciences College of Sciences and Human StudiesPrince Mohammad Bin Fahd UniversityKhobar31952Saudi Arabia

This paper presents a comprehensive study that includes the sizing and power flow by series and parallel inverters in a distributed generation system(DGs)that integrates the system of hybrid wind photovoltaic with a unified power quality conditioner(UPQC).In addition to supplying active power to the utility grid,the system of hybrid wind photovoltaic functions as a UPQC,compensating reactive power and suppressing the harmonic load ***,the load is supplied with harmonic-free,balanced and regulated output *** PVWind-UPQC is established on a dual compensation scheme,the series inverter works like a sinusoidal current source,while the parallel inverter works like a sinusoidal voltage ***,a smooth alteration from interconnected operating modes to island operating modes and vice versa can be achieved without load voltage *** PV-Wind-UPQC inverters handle the energy generated through the hybrid wind photovoltaic system and the energy demanded through the load,the converters should be sized cautiously.A detailed study of the flow of power via the PV-Wind-UPQC is imperative to gain a complete understanding of the system operation and the proper design of the ***,curves that allow the sizing of the power converters according to the power flow via the converters are presented and *** results are presented to assess both steady state and dynamic performances of the grid connected hybrid system of *** investigation is verified by simulating and analyzing the results with Matlab/Simulink.

关键词： Photovoltaic wind turbine unified power quality conditioner power flow distributed generation system

Factors associated with changes in physical activity and sedentary behaviour during one year among university-based young adults

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Sports Medicine and Health Science 2021年第4期3卷 236-242页

作者： riaz Uddin Nicola W.Burton Asaduzzaman khan School of Health and Rehabilitation Sciences The University of QueenslandAustralia Institute for Physical Activity and Nutrition(IPAN) School of Exercise and Nutrition ScienceDeakin UniversityAustralia School of Applied Psychology Griffith UniversityAustralia School of Human Movement and Nutrition Sciences The University of QueenslandAustralia

The purpose of this study was to identify correlates of changes in physical activity(PA)and sedentary behaviour(SB)among university-based young adults in *** were from a 1-year prospective study with 2 assessment points(baseline n=573,20.7±1.35 years,45%female;retention rate 69%,analytical sample=395).Participants completed a self-administered written survey on PA,SB,health and lifestyle be-haviours,and *** in PA were categorised as:negligible(±60 min/week decrease,or>60 min/week *** in SB were categorised as negligible(±120 min/week decrease,and>120 min/week *** logistic regression analysis was used to identify the *** quarters(72%)of participants had insufficient PA at both assessment *** those who were sufficiently active at Wave 1,5%became insufficiently active at Wave *** quarter of par-ticipants(23%)had high SB at Wave 1 and Wave *** those who had low SB at Wave 1,16%had high SB at Wave *** male[OR=2.04(95%CI:1.06–3.93)],baseline phone time of>2 h/day[OR=3.14(95%CI:1.04–7.04)]and not participating in organised sports at baseline[OR=2.56(95%CI:1.24–5.29)]were associated with a decrease in PA by>60 min/*** who frequently experienced stress at baseline had higher odds of increasing SB by>120 min/day[OR=1.83(95%CI:1.04–3.23)].SB is more variable than PA over 1 year in university-based young adults in ***,those with high phone time,those not engaging with organised sports,and those with frequent stress may change to a more inactive lifestyle.

关键词： Developing country Health behaviour Health promotion Longitudinal data South Asia University student

Biochemical Mechanism Unlocking Their Potential Role in Salt Tolerance Mechanism of Zizyphus Germplasm

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Phyton-International Journal of Experimental Botany 2023年第5期92卷 1539-1553页

作者： riaz Ahmad Hafiza Muniba Din Muhammad Safina Naz Meryam Manzoor Muhammad Ahsan Altaf Department of Horticulture The University of AgricultureDera Ismail Khan29050Pakistan Department of Horticulture Bahauddin Zakariya UniversityMultan60060Pakistan College of Horticulture Hainan UniversityHaikou570228China

Salinity is one of the major constraints reducing plant growth and *** with poor quality and brackish water to orchards is a major cause of stunted growth and low *** salt tolerance mechanism is one of the complicated genomic characters that is very problematic to develop in fruit trees and becomes much more severe at any growth and developmental *** stress and hormonal imbalances are major constraints causing low biomass *** tree tolerance/sensitivity is chiefly based on the activation of a defense system comprised of super-oxidase dismutase(SOD),peroxidase(POD)and catalases(CAT),non-enzymatic compounds including ascorbic acid,phenolics,flavonoids,stress indicators[i.e.,hydrogen peroxide(H2O2),lipid peroxidation,malondialdehyde(MDA),reactive oxygen species(ROS)and osmolytes containing proline,glycine-betaine(GB),ascorbates(APX),glutathione peroxidase(GPX)and glutathione reductase(GR)].Tolerant genotypes must have higher antioxidant assays to cope with the adverse effects of salinity stress because their defense system had the potential to scavenge toxic ROS and protect from membrane *** work is conducted on agronomic and horticultural crops;however,underutilized fruit crops are still neglected and need serious consideration from plant *** fruit crops especially Zizyphus had excellent nutritional *** current study provides detailed insights into the physiological and biochemical mechanisms of Zizyphus species to cope with the adverse effects of salinity by improving their plant defense *** development of salt-tolerant germplasm is a requisite and can be developed by utilization of physiological,biochemical,and molecular *** of different molecular approaches(i.e.,genome mapping,genome editing,genetic transformation,proteomics,transcriptomics,and metabolites)are effective for higher yield by improving tolerance mechanisms.

关键词： Low yield metabolic developments plant defense system saline water irrigation

Preventing Cloud Network from Spamming Attacks Using Cloudflare and KNN

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Computers, Materials & Continua 2023年第2期74卷 2641-2659页

作者： Muhammad Nadeem Ali Arshad Saman riaz SyedaWajiha Zahra Muhammad Rashid Shahab S.Band Amir Mosavi Department of Computer Science Abasyn UniversityIslamabad44000Pakistan Department of Computer Science National University of TechnologyIslamabad44000Pakistan Future Technology Research Center National Yunlin University of Science and TechnologyDouliuYunlin64002Taiwan Institute of Information Society University of Public ServiceBudapest1083Hungary John von Neumann Faculty of Informatics Obuda UniversityBudapestHungary Institute of Information Engineering Automation and MathematicsSlovak University of Technology in BratislavaSlovakia

Cloud computing is one of the most attractive and cost-saving models,which provides online services to *** computing allows the user to access data directly from any *** nowadays,cloud security is one of the biggest issues that *** types of malware are wreaking havoc on the *** on the cloud server are happening from both internal and external *** paper has developed a tool to prevent the cloud server from spamming *** an attacker attempts to use different spamming techniques on a cloud server,the attacker will be intercepted through two effective techniques:Cloudflare and K-nearest neighbors(KNN)*** will block those IP addresses that the attacker will use and prevent spamming ***,the KNN classifiers will determine which area the spammer belongs *** the end of the article,various prevention techniques for securing cloud servers will be discussed,a comparison will be made with different papers,a conclusion will be drawn based on different results.

关键词： Intrusion prevention system spamming KNN classification spam cyber security botnet

Computationally predicted pathogenic USP9X mutation identified in infertile men does not affect spermatogenesis in mice

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Zoological Research 2022年第2期43卷 225-228页

作者： Wei Liu Yue-Wen Wang Huan Zhang Xue-Feng Xie Ao Ma Qumar Zaman Abdul Rafay Javed Tanveer Abbas Wasim Shah riaz Ahmad Da-Ren Zhao Hui Ma Muhammad Zubair Ranjha khan Qing-Hua Shi Division of Reproduction and Genetics First Affiliated Hospital of USTCHefei National Laboratory for Physical Sciences at MicroscaleCAS Key Laboratory of Innate Immunity and Chronic DiseaseSchool of Basic Medical SciencesDivision of Life Sciences and MedicineBiomedical Sciences and Health Laboratory of Anhui ProvinceCAS Center for Excellence in Molecular Cell ScienceCollaborative Innovation Center of Genetics and DevelopmentUniversity of Science and Technology of ChinaHefeiAnhui 230027China Medical Genetics Research Laboratory Department of BiotechnologyQuaid-i-Azam UniversityIslamabad 45320Pakistan

Infertility is a major health issue,affecting approximately 15%of couples of child-bearing *** nearly half of idiopathic infertility cases are assumed to have a genetic basis,the underlying causes remain largely unkno... 详细信息

关键词： tile assumed USP9X

Optimizing Steering Angle Predictive Convolutional Neural Network for Autonomous Car

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Computers, Materials & Continua 2022年第5期71卷 2285-2302页

作者： Hajira Saleem Faisal riaz Asadullah Shaikh Khairan Rajab Adel Rajab Muhammad Akram Mana Saleh Al Reshan Control Automotive and Robotics Lab Affiliated lab of National Center of Robotics and Automation(NCRA HEC Pakistan)and with the Department of Computer Science and Information TechnologyMirpur University of Science and Technology(MUST)Mirpur Azad Kashmir10250Pakistan College of Computer Science and Information System Najran UniversityNajran61441Saudi Arabia College of Computer Science and Engineering University of South FloridaTampa33620United States

Deep learning techniques,particularly convolutional neural networks(CNNs),have exhibited remarkable performance in solving visionrelated problems,especially in unpredictable,dynamic,and challenging *** autonomous vehicles,imitation-learning-based steering angle prediction is viable due to the visual imagery comprehension of *** this regard,globally,researchers are currently focusing on the architectural design and optimization of the hyperparameters of CNNs to achieve the best *** has proven the superiority of metaheuristic algorithms over the manual-tuning of ***,to the best of our knowledge,these techniques are yet to be applied to address the problem of imitationlearning-based steering angle ***,in this study,we examine the application of the bat algorithm and particle swarm optimization algorithm for the optimization of the CNN model and its hyperparameters,which are employed to solve the steering angle prediction *** validate the performance of each hyperparameters’set and architectural parameters’set,we utilized the Udacity steering angle dataset and obtained the best results at the following hyperparameter set:optimizer,Adagrad;learning rate,0.0052;and nonlinear activation function,exponential linear *** per our findings,we determined that the deep learning models show better results but require more training epochs and time as compared to shallower *** show the superiority of our approach in optimizing CNNs through metaheuristic algorithms as compared with the manual-tuning *** testing was also performed using the model trained with the optimal architecture,which we developed using our approach.

关键词： Bat algorithm convolutional neural network hyperparameters metaheuristic optimization algorithm steering angle prediction

Darboux transformation and exact multisolitons for a matrix coupled dispersionless system

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Communications in Theoretical Physics 2020年第7期72卷 1-7页

作者： H Wajahat A riaz Department of Mathematics China University of Mining and TechnologyBeijing100083China

We present a matrix coupled dispersionless(CD)system.A Lax pair for the matrix CD system is proposed and Darboux transformation is constructed on the solutions of the matrix CD system and the associated Lax *** express an N soliton formula for the solutions of the matrix CD system in terms of *** using properties of the quasideterminants,we obtain some exact solutions,including bright and dark-type solitons,rogue wave and breather solutions of the matrix CD ***,it has been shown that the solutions of the matrix CD system are expressed in terms of solutions to the usual CD system,sine-Gordon equation and Maxwell-Bloch system.

关键词： Matrix coupled dispersionless system Darboux transformation solitons