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Insect Science 2024年第4期31卷 1150-1164页

作者： Feng wang ri-yuan wang De-Bin Zhong Ping Zhao Qing-You Xia Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science City Biological Science Research CenterSouthwest UniversityChongqingChina Century Legend Biotechnology Research Institute(Chongqing)Co. Ltd.ChongqingChina

Extracellular superoxide dismutase(EcSOD)protects tissues from oxidative stress,and thus is considered as a therapeutic agent for many diseases such as atherosclerosis,hypertension,and ***,cost-effective production of bioactive recombinant human EcSOD(rhEcSOD)remains a ***,we developed an efficient strategy for producing active rhEcSOD by transgenic *** was successfully synthesized as homodimers and homotetramers in the middle silk gland and spun into the cocoons with a concentration of 9.48±0.21 mg/*** of rhEcSOD from the cocoons could be conveniently achieved with a purity of 99.50%and a yield of 3.5±0.5 mg/***,N-glycosylation at the only site of N89 in rhEcSOD with 10 types were *** purified rhEcSOD gained the potent enzymatic activity of 4162±293 U/mg after Cu/Zn ions *** importantly,rhEcSOD was capable of penetrating and accumulating in the nuclei of cells to maintain cell morphology and attenuate ultraviolet B-induced cell apoptosis by eliminating reactive oxygen species and inhibiting the C-Jun N-terminal kinase signaling *** results demonstrated that the transgenic silkworm could successfully produce rhEcSOD with enzymatic and biological activities for biomedical applications.

关键词： antioxidant human extracellular superoxide dismutase recombinant expression silkworm silk gland

An inducible constitutive expression system in Bombyx mori mediated by phiC31 integrase

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Insect Science 2021年第5期28卷 1277-1289页

作者： Feng wang Yan-Ting Ji Chi Tian yuan-Cheng wang Shen Xu ri-yuan wang Qian-Qian Yang Ping Zhao Qing-You Xia State Key Laboratory of Silkworm Genome Biology Chongqing Engineering and Technology Research Center for Novel Silk MaterialsBiological Science Research CenterSouthwest UniversityChongqingChina

Inducible gene-expression systems play important roles in gene functional assays in the post-genome *** phage-derived phiC31 integrase,which mediates an irreversible site-specific cassette exchange between the phage attachment site(attP)and the bacterial attachment site(attB),provides a promising option for the construction of a controllable gene-expression ***,we report a phiC3I integrase-mediated promoter flip system(FLIP)for the inducible expression of target genes in silk-worm(Bombyx mori).First,we constructed a FLIP reporter system,in which a BmAct4 promoter with enhanced translational efficiency was flanked by the attB and attP sites in a head-to-head orientation and further linked in a reverse orientation to a DsRed reporter *** coexpression of a C-terminal modified phiC3 I-NLS integrase carrying a simian virus 40(SV40)nuclear localization signal(NLS)effectively flipped the BmAct4 promoter through an attBlattP exchange,thereby activating the downstream expression of DsRed in a silkworm embryo-derived cell line,***,the FLIP system,together with a system continuously expressing the phiC3 I-NLS integrase,was used to construct binary transgenic silkworm *** between FLIP and phiC31-NLS transgenic silkworm lines resulted in the successful flipping of the BmAct4 promoter,with an approximately 39%heritable transformation efficiency in silkworm offispring,leading to the constitutive and high-level expression of DsRed in silkworms,which accounted for approximately 0.81%of the silkworm pupal *** successful development of the FLIP system offers an effective alternative for manipulating gene expression in silkworms and other lepidopteran species.

关键词： inducible gene expression PhiC31 integrase promoter flipping silkworm

Four decades of full-scale nitrous oxide emission inventory in China

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National Science Review 2024年第3期11卷 137-149页

作者： Minqi Liang Zheyan Zhou Peiyang Ren Han Xiao Xu-ri Zhongmin Hu Shilong Piao Hanqin Tian Qing Tong Feng Zhou Jing Wei Wenping yuan School of Atmospheric Sciences Guangdong Province Data Center of Terrestrial and Marine Ecosystems Carbon Cycle Sun Yat-sen University State Key Laboratory of Tibetan Plateau Earth System Resources and Environment Institute of Tibetan Plateau Research Chinese Academy of Sciences Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province Hainan University Sino-French Institute for Earth System Science College of Urban and Environmental SciencesPeking University Schiller Institute for Integrated Science and Society Department of Earth and Environmental Sciences Boston College Institute of Energy Environment and Economy Tsinghua University Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)

China is among the top nitrous oxide（N2O）-emitting countries, but existing national inventories do not provide full-scale emissions including both natural and anthropogenic sources. We conducted a four-decade（1980–2020） of comprehensive quantification of Chinese N2O inventory using empirical emission factor method for anthropogenic sources and two up-to-date process-based models for natural sources. Total N2O emissions peaked at 2287.4（1774.8–2799.9） Gg N2O yr-1in 2018, and agriculture-developed regions, like the East, Northeast, and Central, were the top N2O-emitting regions. Agricultural N2O emissions have started to decrease after 2016 due to the decline of nitrogen fertilization applications, while, industrial and energetic sources have been dramatically increasing after 2005. N2O emissions from agriculture, industry,energy, and waste represented 49.3%, 26.4%, 17.5%, and 6.7% of the anthropogenic emissions in 2020,respectively, which revealed that it is imperative to prioritize N2O emission mitigation in agriculture,industry, and energy. Natural N2O sources, dominated by forests, have been steadily growing from 317.3（290.3–344.1） Gg N2O yr-1in 1980 to 376.2（335.5–407.2） Gg N2O yr-1in 2020. Our study produces a Full-scale Annual N2O dataset in China（FAN2020）, providing emergent counting to refine the current national N2O inventories.

关键词： nitrous oxide four decades anthropogenic sources natural sources

Relationship between preoperative psychological stress and shortterm prognosis in elderly patients with femoral neck fracture

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World Journal of Psychiatry 2024年第6期14卷 838-847页

作者： Wen-Hui Fu Zhi-Long Hu yuan-Jun Liao ri-Jiang Chen Jian-Bin Qiu Wu-Tang Que Wan-Tao wang Wei-Hua Li Wei-Bin Lan Department of Orthopedics Longyan First Affiliated Hospital of Fujian Medical UniversityLongyan 361000Fujian ProvinceChina

BACKGROUND Older adults are at high risk of femoral neck fractures(FNFs).Elderly patients face and adapt to significant psychological burdens,resulting in different degrees of psychological stress *** hip replacement is the preferred treatment for FNF in elderly patients;however,some patients have poor postoperative prognoses,and the underlying mechanism is *** speculated that the postoperative prognosis of elderly patients with FNF may be related to preoperative psychological *** To explore the relationship between preoperative psychological stress and the short-term prognosis of elderly patients with *** In this retrospective analysis,the baseline data,preoperative 90-item Symptom Checklist score,and Harris score within 6 months of surgery of 120 elderly patients with FNF who underwent total hip arthroplasty were *** analyzed the indicators of poor short-term postoperative prognosis and the ability of the indicators to predict poor prognosis and compared the correlation between the indicators and the Harris *** Anxiety,depression,garden classification of FNF,cause of fracture,FNF reduction quality,and length of hospital stay were independent influencing factors for poor short-term postoperative prognoses in elderly patients with FNF(Pris scores(r=-0.523,-0.625,and-0.554;all P<0.001).CONCLUSION Preoperative anxiety,depression,and somatization are correlated with poor short-term prognosis in elderly patients with FNF and warrant consid

关键词： Psychological stress Old age Femoral neck fracture Hip replacement Short-term prognosis Correlation

Modulated noble metal/2D MOF heterostructures for improved hydrogen storage of MgH_(2)

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Rare Metals 2024年第4期43卷 1672-1685页

作者： Zeng-Yi Li Li-Xian Sun Fen Xu Yu-Mei Luo Yong-Peng Xia Sheng Wei Chen-Chen Zhang ri-Guang Cheng Chao-Feng Ye Meng-yuan Liu Ju-Lan Zeng Zhong Cao Hong-Ge Pan School of Material Science and Engineering Guilin University of Electronic TechnologyGuilin541004China School of Chemistry and Chemical Engineering Changsha University of Science and TechnologyChangsha410114China Institute of Science and Technology for New Energy Xi’an Technological UniversityXi’an710021China

The conjugation of external species with twodimensional(2D)materials has broad application *** this study,we have explored the potential of noble metal/2D MOF heterostructures in hydrogen ***,the MgH_(2)-Ni-MOF@Pd system has shown remarkable hydrogen desorption/sorption performances,starting to liberate hydrogen at 1810C,which is 2300C lower than that of pristine *** the catalytic effect of Ni-MOF@Pd,the dehydrogenation apparent activation energy of MgH2 is noticeably decreased from(133.5±17.5)to(34.58±1.87)kJ·moL^(-1),and the hydrogenation apparent activation energy is reduced from(70.41±7.43)to(25.78±4.64)kJ·moL^(-1),which is lowered by 63.4%.The fully-dehydrogenated MgH2-NiMOF@Pd composite rapidly uptakes hydrogen,with 2.62wt%at 100℃and 6.06 wt%at 150℃within 300 s,*** mechanism analysis of MgH2 catalyzed by Ni-MOF@Pd has revealed that the transformation of Mg_2Ni and Mg_2NiH_4 could act as a"hydrogen pump",providing numerous channels for fast diffusion and transport of hydrogen ***,in the dehydrogenation process,the element Pd reacts with MgH_(2)to form the MgPd alloy phase,which makes MgH_(2)take precedence to decompose through the Mg-Pd alloy rather than self-decomposition,further reducing thermal stability and improving de/hydrogenation *** synergistic effect of Mg-Pd,Mg_2Ni,and the special ultra-thin 2D sheet structure of the additive is the main reason for the good hydrogen storage property of MgH_(2)-Ni-MOF@*** findings provide inspiration for designing efficient multifunctional additives with unique morphologies to optimize the hydrogen desorption/sorption behaviors of hydrogen storage materials.

关键词： Hydrogen storage Magnesium hydride 2D metal-organic framework nanosheets Ni/Pd Synergistic catalysis

Neoadjuvant nivolumab with or without platinum-doublet chemotherapy based on PD-L1 expression in resectable NSCLC(CTONG1804):a multicenter open-label phase II study

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Signal Transduction and Targeted Therapy 2024年第1期9卷 249-258页

作者： Si-Yang Liu Song Dong Xue-Ning Yang ri-Qiang Liao Ben-yuan Jiang Qun wang Xiao-Song Ben Gui-Bin Qiao Jun-Tao Lin Hong-Hong Yan Li-Xu Yan Qiang Nie Hai-Yan Tu Bin-Chao wang Jin-Ji Yang Qing Zhou Hong-Rui Li Ke Liu Wendy Wu Si-Yang Maggie Liu Wen-Zhao Zhong Yi-Long Wu Guangdong Lung Cancer Institute Guangdong Provincial Key Laboratory of Translational Medicine in Lung CancerGuangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouChina Department of Thoracic Surgery Zhongshan HospitalFudan UniversityShanghaiChina Department of Thoracic Surgery Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouChina Department of Pathology Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouChina Fujian Key Laboratory of Advanced Technology for Cancer Screening and Early Diagnosis FuzhouChina Berry Oncology Corporation FuzhouChina Chinese Thoracic Oncology Group(CTONG) GuangzhouChina Department of Hematology The First Affiliated HospitalJinan UniversityGuangzhouChina

This prospective multicenter phase II study evaluated the clinical efficacy of neoadjuvant nivolumab-exclusive(N)and nivolumab–chemotherapy(N/C)combinations based on PD-L1 *** patients exhibited resectable clinical stage IIA–IIIB(AJCC 8th edition)NSCLC without EGFR/ALK *** received either mono-nivolumab(N)or nivolumab+nabpaclitaxel+carboplatin(N/C)for three cycles based on PD-L1 *** primary endpoint was the major pathological response(MPR).Key secondary endpoints included the pathologic complete response(pCR),objective response rate(ORR),and event-free survival(EFS).Baseline PD-L1 expression and perioperative circulating tumor DNA(ctDNA)status were correlated with pCR and ***-two patients were enrolled,with 46 undergoing *** MPR was 50.0%(26/52),with 25.0%(13/52)achieving pCR,and 16.7%and 66.7%for patients with PD-L1≥50%in N and N/C groups,***(25.0%)patients experienced grade 3 or higher immune-related adverse events during neoadjuvant *** with post-neoadjuvant ctDNA negativity was more likely to have pCR(39.1%)compared with those remained positive(6.7%,odds ratio=6.14,95%CI 0.84-Inf,p=0.077).With a median follow-up of 25.1 months,the 18-month EFS rate was 64.8%(95%CI 51.9–81.0%).For patients with ctDNA–***+,the 18m-EFS rate was 93.8%vs 47.3%(HR,0.15;95%CI 0.04,0.94;p=0.005).Immunochemotherapy may serve as an optimal neoadjuvant treatment even for patients with PD-L1 expression≥50%.ctDNA negativity following neoadjuvant treatment and surgery could help identify superior pathological and survival benefits,which requires further confirmation in a prospective clinical trial(NCT04015778).

关键词： chemotherapy NSCLC treatment

The cytochrome P4501A1(CYP1A1)inhibitor bergamottin enhances host tolerance to multidrug-resistant Vibrio vulnificus infection

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Chinese Journal of Traumatology 2024年第5期27卷 295-304页

作者： Ruo-Bai Qiao Wei-Hong Dai Wei Li Xue Yang Dong-Mei He Rui Gao Yin-Qin Cui ri-Xing wang Xiao-yuan Ma Fang-Jie wang Hua-Ping Liang The College of Emergency and Trauma Hainan Medical UniversityHaikou571199China The First Research Department State Key Laboratory of Trauma and Chemical PoisoningArmy Medical Center(Daping Hospital)Army Medical UniversityChongqing400042China Emergency Department The Second Affiliated HospitalHainan Medical UniversityHaikou570311China

Purpose:Vibrio vulnificus (V. Vulnificus) infection is characterized by rapid onset, aggressive progression, and challenging treatment. Bacterial resistance poses a significant challenge for clinical anti-infection treatment and is thus the subject of research. Enhancing host infection tolerance represents a novel infection prevention strategy to improve patient survival. Our team initially identified cytochrome P4501A1 (CYP1A1) as an important target owing to its negative modulation of the body''s infection tolerance. This study explored the superior effects of the CYP1A1 inhibitor bergamottin compared to antibiotic combination therapy on the survival of mice infected with multidrug-resistantV. Vulnificus and the protection of their vital ***:An increasing concentration gradient method was used to induce multidrug-resistantV. Vulnificus development. We established a lethal infection model in C57BL/6J male mice and evaluated the effect of bergamottin on mouse survival. A mild infection model was established in C57BL/6J male mice, and the serum levels of creatinine, urea nitrogen, aspartate aminotransferase, and alanine aminotransferase were determined using enzyme-linked immunosorbent assay to evaluate the effect of bergamottin on liver and kidney function. The morphological changes induced in the presence of bergamottin in mouse organs were evaluated by hematoxylin and eosin staining of liver and kidney tissues. The bacterial growth curve and organ load determination were used to evaluate whether bergamottin has a direct antibacterial effect on multidrug-resistantV. Vulnificus. Quantification of inflammatory factors in serum by enzyme-linked immunosorbent assay and the expression levels of inflammatory factors in liver and kidney tissues by real-time quantitative polymerase chain reaction were performed to evaluate the effect of bergamottin on inflammatory factor levels. Western blot analysis of IκBα, phosphorylated IκBα, p65, and phosphorylated p65 prot

关键词： Multidrug-resistant Vibrio vulnificus Bergamottin CYP1A1 inhibitors Infection tolerance

钼酸锰/氮化碳纳米复合材料修饰电极检测甲硝唑

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分析化学 2024年第2期52卷 220-233页

作者：刘蕊武天恒刘日嘉孙源哈尔滨商业大学药学院药物工程技术研究中心

采用水热法制备了钼酸锰/氮化碳纳米复合材料(MnMoO4/g-C3N4)，通过X射线衍射(XRD)、傅里叶变换红外光谱(FT-IR)和扫描电镜(SEM)等表征了复合材料的表面形貌特征及其结构。利用滴涂法将MnMoO4/g-C3N4修饰于玻碳电极(GCE)表面，构建了检... 详细信息

采用水热法制备了钼酸锰/氮化碳纳米复合材料(MnMoO4/g-C3N4)，通过X射线衍射(XRD)、傅里叶变换红外光谱(FT-IR)和扫描电镜(SEM)等表征了复合材料的表面形貌特征及其结构。利用滴涂法将MnMoO4/g-C3N4修饰于玻碳电极(GCE)表面，构建了检测甲硝唑(MNZ)的电化学传感器。采用循环伏安法(CV)和差分脉冲伏安法(DPV)研究了MnMoO4/g-C3N4/GCE电极的电化学行为，考察了pH值和扫描速率等参数对电流响应的影响。在优化条件下，此修饰电极检测MNZ的线性范围为0.5～2400μmol/L，检出限(LOD, 3σ/k)为1.33 nmol/L，并具有良好的选择性、稳定性和重现性。将此传感器用于检测鸡蛋和牛奶中的MNZ，加标回收率分别在97.7%～103.7%和96.9%～102.4%之间，相对标准偏差(RSD)为1.1%～2.2%，表明所制备的MnMoO4/g-C3N4/GCE传感器可用于实际食品中MNZ的检测，为食品安全监管提供了新方法。

关键词：钼酸锰类石墨相氮化碳电化学检测甲硝唑

来源：博看期刊

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基于多目标筛选堆叠回归的光谱反射率重建

光谱学与光谱分析 2024年第10期44卷 2988-2992页

作者：李日浩马媛张伟峰华南农业大学数学与信息学院广东广州510642

物体的光谱反射率完全决定了其物体色,因此研究光谱反射率对于色彩信息要求较高的行业具有重大意义。直接获取光谱反射率需要精密且昂贵的设备,而通过建立模型,由低成本的数码相机等设备获取的RGB响应值去预测光谱反射率,可以大大降低... 详细信息

物体的光谱反射率完全决定了其物体色,因此研究光谱反射率对于色彩信息要求较高的行业具有重大意义。直接获取光谱反射率需要精密且昂贵的设备,而通过建立模型,由低成本的数码相机等设备获取的RGB响应值去预测光谱反射率,可以大大降低成本。基于回归方法的光谱反射率重建算法受到广泛关注,其核心是建立RGB向量到光谱反射率向量间的映射关系。对于大多数物体而言,其表面的光谱反射率曲线具有平滑属性,因此,光谱反射率分量之间具有一定的相关性。而已有的算法都是对光谱反射率向量每一个维度独立地建立预测模型,没有将光谱反射率分量之间的相关性利用起来。与传统的单输出回归方法不同,多目标堆叠回归方法通过将首次预测输出值重新注入输入端来利用输出端之间的相关性。基于多目标堆叠回归的光谱反射率重建取得了重要的进展;然而,传统的多目标堆叠回归方法存在着易受首次预测输出值误差影响的问题。针对这一问题,提出一种新的多目标堆叠方法,对于首次预测输出值进行筛选,从中选出误差较小的部分作为输入,以此来保证下一步建立的模型精度。该筛选方法可以在不知道真实值的情况下,极大程度地保留误差较低的部分样本。实验数据集来源为ICVL高光谱图像数据库,评价指标为均方根误差与色度误差。实验结果表明,所提出的多目标筛选堆叠回归可以有效克服传统多目标堆叠回归所存在的问题,做到比无堆叠时的误差更小,说明提出的方法可以有效地利用光谱反射率分量之间的相关性。

关键词：光谱反射率重建多目标堆叠回归筛选条件非线性拟合

长期定位施肥对土壤铜、锌形态转化及其空间分布的影响

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水土保持学报 2011年第5期25卷 140-143,167页

作者：韩晓日袁程王月杨劲峰李娜张欣昕沈阳农业大学土地与环境学院农业部东北土壤与环境重点开放实验室沈阳110866

采用改进的BCR连续提取法,对沈阳农业大学棕壤肥料长期定位试验地31年不同施肥处理土壤铜和锌含量变化及其空间变异规律进行研究。结果表明:与试验前相比,耕层(0-20cm)土壤各处理水溶态铜、弱酸溶态铜和残渣态铜含量都有不同程度的减少... 详细信息

采用改进的BCR连续提取法,对沈阳农业大学棕壤肥料长期定位试验地31年不同施肥处理土壤铜和锌含量变化及其空间变异规律进行研究。结果表明:与试验前相比,耕层(0-20cm)土壤各处理水溶态铜、弱酸溶态铜和残渣态铜含量都有不同程度的减少,可还原态铜和可氧化态铜含量在化肥区有所降低,在有机肥区有所增加;残渣态锌含量明显减少,其他形态锌含量有不同程度的增加。耕层各形态铜分布趋势因处理不同而不同,锌以残渣态为主。在空间分布上,2种元素弱酸溶态、可还原态和可氧化态含量都是随土层的加深而减少。可氧化态铜与有效态铜关系最密切;可还原态锌对有效态锌的贡献最大。

关键词：棕壤长期定位施肥铜锌形态