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Effects of dietary cottonseed meal protein hydrolysate on growth,antioxidants and immunity of Chinese mitten crab Eriocheir Sinensis

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Journal of Oceanology and Limnology 2020年第3期38卷 869-882页

作者： CHENG Huihui LIU Wenbin YUAN Xiangyang jia Erteng zhang Dingdong jiaNG Guangzhen Key Laboratory of Aquatic Nutrition and Feed Science of jiangsu Province National Experimental Teaching Center for Animal ScienceCollege of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095China

One hundred and sixty crabs(average initial weight:51.32±0.08 g)were fed with four experimental diets containing cottonseed meal protein hydrolysate(CPH)at 0%(CPH0,control),0.3%(CPH0.3),0.6%(CPH0.6),and 1.2%CPH(CPH1.2).The experiment results show that no difference was observed in specific growth rate and survival rate of crabs fed with CPH *** content of crabs fed with CPH0.6 diet was significantly reduced than that of the CPH0 *** dismutase and catalase activities of crabs fed with CPH0.6 diet were significantly increased and the difference was not significant between the CPH0.3 and CPH0.6 *** content of CPH0.3 group was significantly lower than that of the CPH0 ***,alkaline phosphatase,and acid phosphatase activities of CPH0.3 diet crabs were significantly higher than that of the CPH0 ***-pyruvic transaminase activity of crabs fed with CPH0.3 diet was significantly decreased compared to the CPH0 *** relative expression levels of Toll1,Toll2,MyD88,LITAF,and ILF-2 of crabs fed with CPH0.3 diet were significantly higher than that of the CPH0 *** expression level of SOCS2 showed an opposite *** CPH perfusion,the expression levels of SOCS2 and Toll1 in intestine at time 3 h and SOCS2 in hepatopancreas at time 18 h increased significantly to the highest *** expression level of Toll2,MyD88,LITAF,decreased at times 6 h,6 h,12 h,respectively,then increased ***,supplementation of dietary CPH could improve antioxidant capacity and immune function;the appropriate supplement dosage of CPH for crab could be 0.3%-0.6%.Furthermore,the short-term CPH stimulation could significantly increase or decline the expression levels of immune-related genes at different times after CPH perfusion.

关键词： Eriocheir sinensis cottonseed meal protein hydrolysate growth body composition immunity antioxidant

Dynamic performance and energy efficiency of reflective and insulative composite coating on building exterior wall

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Building Simulation 2023年第12期16卷 2245-2259页

作者： Guangpeng zhang Huijun Wu jia Liu jianming Yang Huakun Huang Yujie Ding Lei Xie School of Civil Engineering Guangzhou UniversityGuangzhou 510006China Guangdong Provincial Key Laboratory of Building Energy Efficiency and Application Technologies Guangzhou 510006China School of Automation Guangdong Polytechnic Normal UniversityGuangzhou 510665China School of Computer Science and Cyber Engineering Guangzhou UniversityGuangzhou 510006China

Reflective and insulative composite coatings are a new energy-saving material with high solar reflectance and extremely low thermal conductivity for *** optimization and impact of high solar reflectance and low thermal conductivity on the insulating capacity of walls remain *** work investigates the dynamic thermal performance and energy efficiency of a reflective and insulative composite coating in regions with hot summer and warm winter.A simplified thermal resistance-heat capacitance model of an exterior building wall is established to predict thermal *** dynamic temperature and heat flow of the wall are predicted to reduce heat loss through the interior surface of the wall and compared to the conventional *** specific impact of the thermal conductivity and solar reflectance of the coating on the heat loss is further investigated to minimize heat loss of the *** research shows that the composite coating shows better performance on adjusting outdoor climate change than the other *** with cement,it reduces the maximum temperature of the exterior surface of the wall by 7.45°C,and the heat loss through the interior surface of the wall by 38%.The heat loss is reduced with the increase of solar reflectance and the reduction of thermal *** results can provide a useful reference and guidance for the application of reflective and insulative composite coating on building exterior wall to promote their energy-saving use on building envelopes.

关键词： reflective and insulative composite coating exterior wall building insulation solar reflectance thermal conductivity

国产神威环境下Athread代码生成工具的设计与开发

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计算机工程与设计 2024年第2期45卷 633-640,F0003页

作者：刘加伟郭强庄园张海红王利曾云辉齐鲁工业大学(山东省科学院)计算机科学与技术学部山东济南250014 齐鲁工业大学(山东省科学院)山东省计算中心(国家超级计算济南中心) 山东济南250103

针对国产神威环境下众核代码编写工作量大的问题,设计实现一个可以将核心段的串行代码自动转换为Athread代码的工具。采用Rust语言进行词法和语法分析,面向不同数组维度的Fortran和C语言程序,基于主程序调用master程序再由master程序调... 详细信息

针对国产神威环境下众核代码编写工作量大的问题,设计实现一个可以将核心段的串行代码自动转换为Athread代码的工具。采用Rust语言进行词法和语法分析,面向不同数组维度的Fortran和C语言程序,基于主程序调用master程序再由master程序调用slave程序的三层模板程序架构,集成常用众核优化方法的代码框架。经过实验分析,采用该自动转换工具生成的Athread代码相较于人工编写的OpenACC*加速的程序有更高的加速比,特别是对多个核心段进行众核化加速时的加速比相差15%,验证该转换工具具有很好的实用性。

关键词：加速线程库代码生成模板引擎语法分析众核处理器神威·太湖之光高性能计算

Light-induced variation in environmentally persistent free radicals and the generation of reactive radical species in humic substances

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Frontiers of Environmental Science & Engineering 2020年第6期14卷 171-180页

作者： Yafang Shi Yunchao Dai Ziwen Liu Xiaofeng Nie Song Zhao Chi zhang Hanzhong jia College of Natural Resources and Environment Northwest A&F UniversityYangling 712100China Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China Ministry of AgricultureYangling 712100China

Environmentally persistent free radicals(EPFRs)in humic substances play an essential role in soil geochemical *** is known to induce EPFRs formation for dissolved organic matter in aquatic environments;however,the impacts of light irradiation on the variation of EPFRs in soil humic substances remain *** this study,humic acid,fulvic acid,and humin were extracted from peat soil and then in situ irradiated using simulated *** paramagnetic resonance spectroscopy results showed that with the increasing irradiation time,the spin densities and g-factors of humic substances rapidly increased during the initial 20 min and then gradually reached a *** irradiation for 2h,the maximum spin density levels were up to 1.63×10^17,2.06×10^17,and 1.77×10^×10^17 spins/g for the humic acid,fulvic acid,and humin,*** the superoxide radicals increased to 1.05×10^l4-1.46×10^14spins/g while the alkyl radicals increased to 0.47×10^14-1.76×10^14 spins/*** light-induced EPFRs were relatively unstable and readily returned back to their original state under dark and oxic *** positive correlations were observed between the concentrations of EPFRs and reactive radical species(R2=0.65-0.98,/?ributed to the formation of reactive radical *** findings indicate that under the irradiation humic substances are likely to be more toxic and reactive in soil due to the formation of EPFRs.

关键词： Peat Humic substances Environmentally persistent free radicals Light irradiation Reactive radical species

胡黄连苷Ⅱ通过抑制NF-κB/MAPK信号通路减轻过敏性哮喘大鼠炎症反应

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现代生物医学进展 2024年第10期24卷 1818-1824,1868页

作者：周丹金发光潘蕾李立宏张媛董佳佳空军军医大学第二附属医院急诊科陕西西安710032 空军军医大学第二附属医院呼吸与危重症医学科陕西西安710032

目的:探究胡黄连苷Ⅱ对过敏性哮喘大鼠炎症反应以及NF-κB/MAPK信号通路的影响。方法:采用卵清白蛋白致敏和雾化吸入激发构建过敏性哮喘大鼠模型。将建模成功的大鼠随机分为模型组、胡黄连苷Ⅱ组和C16-PAF组,另取未建模健康的大鼠作为... 详细信息

目的:探究胡黄连苷Ⅱ对过敏性哮喘大鼠炎症反应以及NF-κB/MAPK信号通路的影响。方法:采用卵清白蛋白致敏和雾化吸入激发构建过敏性哮喘大鼠模型。将建模成功的大鼠随机分为模型组、胡黄连苷Ⅱ组和C16-PAF组,另取未建模健康的大鼠作为对照组,每组8只。胡黄连苷Ⅱ组大鼠在造模成功后每日给予灌胃剂量为80 mg/kg的胡黄连苷Ⅱ,激活剂组(C16-PAF组)大鼠每日给予灌胃剂量为80 mg/kg的胡黄连苷Ⅱ和50μL浓度为4μmol/L的C16-PAF溶液(MAPK激活剂),共给药14天。模型组大鼠同时给予灌胃等量的生理盐水,对照组大鼠分别在造模和给药同期给予腹腔注射、雾化吸入和灌胃等量的生理盐水。采用全身体积描记法记录大鼠肺功能指标FVC、PEF、FEV0.3以及FEV0.3/FVC。通过苏木精-伊红(HE)染色检测大鼠肺组织病理学变化。采用瑞氏染色分析大鼠肺泡灌洗液(BALF)中白细胞、淋巴细胞、嗜酸性粒细胞和中性粒细胞计数。采用ELISA法检测大鼠血清中OVA-Ig E和ET-1水平和BALF中炎性因子IL-4、IL-5、IL-13和IFN-γ水平。采用免疫组织化学染色法分析大鼠肺组织中p-p38MAPK和p-NF-κBp65表达水平。采用Western blot检测大鼠肺组织NF-κB/MAPK信号通路相关蛋白表达水平。结果:对照组大鼠肺组织基本结构和形态均正常,未见炎性细胞浸润和组织病变;模型组大鼠肺组织出现明显的炎性细胞浸润,支气管腔壁显著增厚、管腔缩小,肺泡壁增厚且破坏严重;胡黄连苷Ⅱ组大鼠肺组织炎性细胞浸润、肺泡和支气管结构损伤改善明显;C16-PAF组大鼠肺组织出现与模型组大鼠类似的组织损伤情况,但严重程度稍微降低。与对照组比较,模型组大鼠的FVC、FEV0.3、FEV0.3/FVC和PEF值均降低(P0.05)。结论:胡黄连苷Ⅱ可能通过抑制NF-κB/MAPK信号通路减轻过敏性哮喘大鼠炎症反应进程。

关键词：胡黄连苷Ⅱ 过敏性哮喘炎症 NF-κB/MAPK信号通路

Periodontitis as a promoting factor of T2D: current evidence and mechanisms

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International Journal of Oral Science 2023年第2期15卷 217-228页

作者： Yuan Su Leilei Ye Chen Hu Yanli zhang jia Liu Longquan Shao Stomatology Center Shunde HospitalSouthern Medical University(The First People's Hospital of Shunde)FoshanChina Stomatological Hospital School of StomatologySouthern Medical University Department of Stomatology Nanfang HospitalSouthern Medical UniversityGuangzhouChina

Periodontitis is an infectious disease caused by an imbalance between the local microbiota and host immune ***,periodontitis is closely related to the occurrence,development,and poor prognosis of T2D and is recognized as a potential risk factor for *** recent years,increasing attention has been given to the role of the virulence factors produced by disorders of the subgingival microbiota in the pathological mechanism of T2D。

关键词： prognosis tit Periodon

Trehalose-6-phosphate phosphatases are involved in trehalose synthesis and metamorphosis in Bactrocera minax

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Insect Science 2022年第6期29卷 1643-1658页

作者： jia Wang Huan Fan Ying Li Tong-Fang zhang Ying-Hong Liu College of Plant Protection Southwest UniversityChongqingChina College of Food Science Southwest UniversityChongqingChina

Trehalose is the principal sugar circulating in the hemolymph of insects,and trehalose synthesis is catalyzed by trehalose-6-phosphate synthase(TPS)and trehalose-6-phosphate phosphatase(TPP).Insect TPS is a fused enzyme containing both TPS do-main and TPP ***,many insects do not possess TPP genes as TPSs have re-placed the function of ***,TPPs are widely distributed across the dipteran insects,while the roles they play remain largely *** this study,3 TPP genes from notorious dipteran pest Bactrocera minax(BmiTPPB,BmiTPPCl,and BmiTPPC2)were identified and *** different temporal-spatial expression patterns of 3 BmiTPPs implied that they exert different functions in *** BmiTPPs were heterologously expressed in yeast cells,and all purified proteins exhibited enzy-matic activities,despite the remarkable disparity in performance between BmiTPPB and *** interference revealed that all BmiTPPs were successfully downregulated after double-stranded RNA injection,leading to decreased trehalose content and increased glucose ***,suppression of BmiTPPs significantly affected expression of down-stream genes and increased the mortality and malformation ***,these results indicated that all 3 BmiTPPs in *** are involved in trehalose synthesis and ***,these genes could be evaluated as insecticidal targets for managing ***,andevenforotherdipteranpests.

关键词： Bactrocera minax heterologous expression metamorphosis trehalose trehalose-6-phosphate phosphatase RNA interference

Electrical characterization of an individual nanowire using flexible nanoprobes fabricated by atomic force microscopy-based manipulation

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Nanotechnology and Precision Engineering 2023年第4期6卷 55-65页

作者： Yilin Wang Enxiu Wu Jirui Liu Mengke jia Rui zhang Sen Wu State Key Laboratory of Precision Measuring Technology and Instruments Tianjin UniversityTianjin 300072China

Nanowires have emerged as promising one-dimensional materials with which to construct various nanocircuits and ***,measuring the electrical properties of individual nanowires directly remains challenging because of their small size,thereby hindering the comprehensive understanding of nanowire-based device performance.A crucial factor in achieving reliable electrical characterization is establishing well-determined contact conditions between the nanowire sample and the electrodes,which becomes particularly difficult for soft *** here is a novel technique for measuring the conductivity of an individual nanowire with the aid of automated nanomanipulation using an atomic force *** this method,two nanowire segments cut from the same silver nanowire are positioned onto a pair of gold electrodes,serving as flexible nanoprobes to establish controllable contact with the *** changing the contact points along the nanowire sample,conductivity measurements can be performed on different regions,thereby eliminating the influence of contact resistance by analyzing multiple current–voltage *** this approach,the resistivity of a 100-nm-diameter silver nanowire is determined to be 3.49×10^(−8)Ωm.

关键词： Electrical characterization Nanowire Flexible nanoprobe Nanomanipulation AFM

Single-electromagnet levitation for density measurement and defect detection

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Frontiers of Mechanical Engineering 2021年第1期16卷 186-195页

作者： Yuhan jia Peng ZHAO Jun XIE Xuechun zhang Hongwei ZHOU jianzhong FU The State Key Laboratory of Fluid Power and Mechatronic Systems Zhejiang UniversityHangzhou 310027China Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province Zhejiang UniversityHangzhou 310027China jiangsu jianghuai Magnetic Industry Co. Ltd.Huaian 211700China Tederic Machinery Co. Ltd.Hangzhou 311224China

This paper presents a single-electromagnet levitation device to measure the densities and detect the internal defects of antimagnetic *** experimental device has an electromagnet in its lower part and a pure iron core in the upper *** the electromagnet is activated,samples can be levitated stably in a paramagnetic *** with traditional magnetic levitation devices,the single-electromagnet levitation device is *** currents,electromagnet shapes,and distances between the electromagnet and iron core are used in the experiment depending on the type of *** magnetic field formed by the electromagnet is *** the concentration of the MnCl aqueous solution is 3 mol/L,the measuring range of the single-electromagnet levitation device ranges from 1.301 to 2.308 g/***,with the same concentration of MnCl aqueous solution(3 mol/L),the measuring range of a magnetic levitation device built with permanent magnets is only from 1.15 to 1.50 g/*** single-electromagnet levitation device has a large measuring range and can realize accurate density measurement and defect detection of high-density materials,such as glass and aluminum alloy.

关键词： single-electromagnet electromagnetic levitation density measurement defect detection