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Study on the Interaction of Vitamin B12 with DNA by Spectroscopy and Electrochemical Methods

Chinese Journal of Chemistry 2011年第7期29卷 1353-1358页

作者： hajian, reza Zafari, Mitra Department of Chemistry Islamic Azad University Branch of Gachsaran Gachsaran 75818-63876 Iran

The Interaction between vitamin B12 （VBI2） and fish sperm DNA was investigated in physiological buffer （pH 7.4） using the methylene blue （MB） dye as a spectral probe by spetcrophotometery, viscosity measurements and cyclic voltammetry. The apparent binding constant of vitamin B12 with DNA was found to be 3.2 × 10^5 mol^-1·L. The voltammetric behavior of vitamin B12 has been investigated at glassy carbon electrode using cyclic voltammetry. Thermodynamic parameters including △H^0, △S^0 and △G^0 for the interaction between VB12 and DNA have determined as -2.3 × 10^4, 27.54 and -3.1 × 10^4j·mol^-l·K^-1 respectively. One indication of DNA binding mode with VBI2 was the change in viscosity when a small molecule associates with DNA. The diffusion coefficients of VB12 in the absence （D0）f and presence of DNA （D0）b was calculated as 5.04 × 10^-6 and 1.13 × 10^-6 cm^2·s^-1 respectively. The results indicated that vitamin B12 can bind to DNA and the major binding mode was intercalative binding.

关键词： vitamin B 12 fish sperm DNA interaction spectrophotometry cyclic voltammetry viscometry

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Fabrication of an electrochemical sensor for determination of doxorubicin in human plasma and its interaction with DNA

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Journal of Pharmaceutical Analysis 2017年第1期7卷 27-33页

作者： reza hajian Zahra Tayebi Nafiseh Shams Young Researchers and Elite Club Gachsaran Branch Islamic Azad University 75818-63876 Gachsaran Iran Department of Chemistry College of Science Gachsaran Branch Islamic Azad University 75818-63876 Gachsaran Iran Institute of Advanced Technology Universiti Putra Malaysia 43400 Serdang Selangor Malaysia

In this work, an electrochemical sensor was fabricated for determination of an anthracycline, doxorubicin(DOX) as a chemotherapy drug in plasma based on multi-walled carbon nanotubes modified platinum electrode(Pt/MWCNTs). DOX was effectively accumulated on the surface of modified electrode and generated a pair of redox peaks at around 0.522 and 0.647 V(vs. Ag/Ag Cl) in Britton Robinson(B-R) buffer(p H 4.0, 0.1 M). The electrochemical parameters including p H, type of buffer, accumulation time, amount of modifier and scan rate were optimized. Under the optimized conditions, there was a linear correlation between cathodic peak current and concentration of DOX in the range of 0.05–4.0 μg/m L with the detection limit of 0.002 μg/m L. The number of electron transfers(n) and electron transfer-coefficient(α) were estimated as 2.0 and 0.25, respectively. The constructed sensor displayed excellent precision, sensitivity, repeatability and selectivity in the determination of DOX in plasma. Moreover, cyclic voltammetry studies of DOX in the presence of DNA showed an intercalation mechanism with binding constant(K_b) of 1.12×10~5L/mol.

关键词： Doxorubicin MWCNTs Electrochemical sensor Human plasma Doxorubicin-DNA interaction

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Electrochemical Study on the Interaction of Irinotecan with Calf Thymus Double Stranded DNA

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Chinese Journal of Chemistry 2012年第3期30卷 738-742页

作者： hajian, reza Huat, Tan Guan Department of Chemistry Faculty of Science University of Malaya 50603 Kuala Lumpur Malaysia

Voltammetric behavior of Irinotecan （CPT-11） was studied in a phosphate buffer （0.002 mol.L^-1, pH 7.5） solution at the hanging mercury drop electrode （HMDE） using cyclic voltammetry （CV）. CPT-11 showed two irreversible cathodic peaks at - 1.01 V and - 1.09 V which involved two electrons and two protons in each reduction step. In addition, the interaction of Irinotecan with double-stranded calf thymus DNA （ds-DNA） was studied by CV at the HMDE employing an irreversible electrochemical equation. As a result of the reaction with ds-DNA, the reduc- tion peaks related to CPT-11 were shifted in a negative direction and the peak currents were decreased. The diffu- sion coefficients of CPT- 11 in the absence （Dr） and presence （Db） of ds-DNA were calculated as 2.8 ×10 5 cm2.s^- 1 and 1.6 × 10^-5 cm2·s^-1 respectively. The binding constant （K=1.0×10^4 L·mol^-1）, and binding site size （s=0.60） of CPT-11 interacting with ds-DNA were obtained simultaneously by non-linear fit analysis. The results demon strate that the main interaction mode of CPT-11 with ds-DNA is electrostatic.

关键词： Irinotecan ds-DNA cyclic voltammetry anticancer drugs interactions

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DNA-binding studies of valrubicin as a chemotherapy drug using spectroscopy and electrochemical techniques

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Journal of Pharmaceutical Analysis 2017年第3期7卷 176-180页

作者： reza hajian Parvin Hossaini Zahra Mehrayin Pei Meng Woi Nafiseh Shams Department of Chemistry College of Science Gachsaran Branch Islamic Azad University Institute of Advanced Technology Universiti Putra Malaysia Department of Chemistry Faculty of Science University of Malaya

In this study, the molecular interactions between valrubicin, an anticancer drug, and fish sperm DNA have been studied in phosphate buffer solution(pH 7.4) using UV–Vis spectrophotometry and cyclic voltammetry techniques. Valrubicin intercalated into double stranded DNA under a weak displacement reaction with methylene blue(MB) molecule in a competitive reaction. The binding constant(kb) of valrubicin-DNA was determined as 1.75×10~3L/mol by spectrophotometric titration. The value of non-electrostatic binding constant(k_t^0) was almost constant at different ionic strengths while the ratio of k_t^0/k_b increased from 4.51% to 23.77%.These results indicate that valrubicin binds to ds-DNA via electrostatic and intercalation *** parameters including ΔH^0, ΔS^0and ΔG^0for valrubicin-DNA interaction were determined as-25.21×10~3k J/mol, 1.55×10~2k J/mol K and-22.03 k J/mol, respectively. Cyclic voltammetry study shows a pair of redox peaks for valrubicin at 0.45 V and 0.36 V(vs. Ag/Ag Cl). The peak currents decreased and peak positions shifted to positive direction in the presence of DNA, showing intercalation mechanism due to the variation in formal potential.

关键词： Valrubicin-DNA interactions Spectroscopy Cyclic voltammetry

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One-Pot Synthesis of Tetrazole Derivatives by Ugi Three Component Reaction

One-Pot Synthesis of Tetrazole Derivatives by Ugi Three Comp...

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The 5th International Conference on Multi-Component Reactions and Related Chemistry(MCR2011)

作者： Ali reza Kazemizadeh Nasrin Hajaliakbari Roghayeh hajian Research Laboratory of MCRs Department of Chemistry Zanjan BranchIslamic Azad University Department of Chemistry Payame Noor University

＜正＞Nowadays many organic compounds can be synthesized by multicomponent reactions（MCR）.Isocyanide-based multicomponent reactions（IMCR） are particularly interesting because they are more versatile and diverse than the remaining *** most IMCR chemistry relates to the classical reactions of Passerini and *** connection

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