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氧化魔芋葡甘露聚糖的添加量对乳清分离蛋白凝胶性能的影响

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食品与发酵工业 2024年第13期50卷 116-122页

作者：谢仁香覃小丽谢勇罗园刘雄西南大学食品科学学院重庆400715 铜仁学院材料与化学工程学院贵州铜仁554300

为改善乳清分离蛋白凝胶性能并提高其营养特性,将氧化魔芋葡甘露聚糖(oxidized konjac glucomannan,OKGM)与乳清分离蛋白(whey protein isolate,WPI)混合制备复合凝胶,研究OKGM添加量(1%、1.5%、2%、2.5%、3%)对WPI凝胶的持水性、质构... 详细信息

为改善乳清分离蛋白凝胶性能并提高其营养特性,将氧化魔芋葡甘露聚糖(oxidized konjac glucomannan,OKGM)与乳清分离蛋白(whey protein isolate,WPI)混合制备复合凝胶,研究OKGM添加量(1%、1.5%、2%、2.5%、3%)对WPI凝胶的持水性、质构、流变特性、热稳定性的影响,并对复合凝胶的形成机理进行了初步探讨。结果表明,OKGM的添加使复合凝胶的持水性(65.59%~93.42%)显著高于纯蛋白凝胶(53.58%);质构分析发现OKGM添加量为2%时凝胶的硬度、胶着性、咀嚼性达到最大值;流变分析发现复合凝胶是弱凝胶,对高频率和高温具有更高的依赖性;热重分析表明OKGM添加量≤1.5%时,OKGM-WPI复合凝胶的热稳定性均高于纯乳清分离蛋白凝胶;表面疏水性和游离巯基分析发现OKGM的添加降低了表面疏水性和游离巯基含量,说明OKGM与WPI发生交联和聚集形成复合凝胶;扫描电镜观察发现复合凝胶结构更为致密、有序。综上,可以通过添加OKGM改善WPI凝胶性能及稳定性。

关键词：氧化魔芋葡甘露聚糖凝胶乳清分离蛋白流变特性稳定性

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迁移体的生物学功能

中国细胞生物学学报 2024年第1期46卷 3-10页

作者：谢任翔姜东俞立膜生物学国家重点实验室北京100084 清华大学–北京大学生命科学联合中心北京100084 北京生物结构前沿研究中心北京100084 清华大学生命科学学院北京100084

迁移体是细胞迁移过程中产生的新型膜性细胞器。细胞迁移时,细胞尾端会产生收缩丝,随后在收缩丝末端或节点处会长出直径为0.5~3.0μm的囊泡结构,该囊泡结构即为迁移体。通过迁移体释放细胞内含物的过程被称为迁移性胞吐。迁移体内富含... 详细信息

迁移体是细胞迁移过程中产生的新型膜性细胞器。细胞迁移时,细胞尾端会产生收缩丝,随后在收缩丝末端或节点处会长出直径为0.5~3.0μm的囊泡结构,该囊泡结构即为迁移体。通过迁移体释放细胞内含物的过程被称为迁移性胞吐。迁移体内富含细胞因子等各类信号分子,存在受损线粒体,并可携带细胞内的蛋白质和核酸。迁移体在多种生命过程中发挥关键作用,可通过携带趋化因子定位至特定空间形成区域信号中心,进而调控胚胎器官形态发生;可携带mRNA等通过吞噬作用在不同细胞间进行物质传递;可吸纳受损线粒体将其排出到细胞外,进而维持细胞稳态。近十年来,研究者系统解析了迁移体在不同生物学过程中的功能,并开发和建立了一系列迁移体研究的工具和方法。该文总结了过去十年里研究者发现的迁移体在信号传递、细胞稳态维持和物质传输方面的功能研究进展,以及现有的迁移体研究工具和方法,为深入理解迁移体和进一步拓展迁移体相关研究提供了参考。

关键词：迁移体迁移性胞吐生物学功能研究方法

Disulfide bridge-targeted metabolome mining unravels an antiparkinsonian peptide

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Acta Pharmaceutica Sinica B 2024年第2期14卷 881-892页

作者： Zhiwu Tong Xiahong xie Huiming Ge Ruihua Jiao Tingting Wang Xincun Wang Wenying Zhuang Gang Hu renxiang Tan State Key Laboratory of Pharmaceutical Biotechnology Institute of Functional BiomoleculesSchool of Life SciencesNanjing UniversityNanjing 210023China State Key Laboratory Cultivation Base for TCM Quality and Efficacy Nanjing University of Chinese MedicineNanjing 210023China Institute of Microbiology Chinese Academy of SciencesBeijing 100101China

Peptides are a particular molecule class with inherent attributes of some small-molecule drugs and macromolecular biologics,thereby inspiring continuous searches for peptides with therapeutic and/or agrochemical ***,the success rate is decreasing,presumably because many interesting but less-abundant peptides are so scarce or labile that they are likely‘overlooked’during the characterization ***,we present the biochemical characterization and druggability improvement of an unprecedented minor fungal RiPP(ribosomally synthesized and post-translationally modified peptide),named acalitide,by taking the relevant advantages of metabolomics approach and disulfide-bridged substructure which is more frequently imprinted in the marketed peptide drug *** is biosynthetically unique in the macrotricyclization via two disulfide bridges and a protease(AcaB)-catalyzed lactamization of AcaA,an unprecedented precursor *** a biosynthetic logic was successfully re-edited for its sample supply renewal to facilitate the identification of the in vitro and in vivo antiparkinsonian efficacy of acalitide which was further confirmed safe and rendered brain-targetable by the liposome encapsulation *** together,the work updates the mining strategy and biosynthetic complexity of RiPPs to unravel an antiparkinsonian drug candidate valuable for combating Parkinson’s disease that is globally prevailing in an alarming manner.

关键词： Fungal RiPPs Biosynthesis Macrocyclic peptide Acalitide Antiparkinsonian

The role of ATF3 in ZnO nanoparticle-induced genotoxicity and cytotoxicity in bronchial epithelial cells

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The role of ATF3 in ZnO nanoparticle-induced genotoxicity an...

Inhibition of RNA polymerase Ⅲ transcription by Triptolide attenuates colorectal tumorigenesis

华东六省一市生物化学与分子生物学会联盟2019年学术与...

作者： Saisai Wei Tiezheng Li renxiang xie Bingqi Ye Jie Xiang Kangli Liu Zhanghui Chen Xiangwei Gao Institute of Environmental Health and Sir Run-Run Shaw Hospitaland Institute of Environmental HealthZhejiang University School of Medicine Affiliated Hospital of Guangdong Medical University Institute of Environmental Medicine Zhejiang University School of Medicine

ZnO nanoparticle(ZnO NP) exposure causes oxidative stress in the respiratory system,leading to pulmonary *** transcription factor 3(ATF3) participates in a variety of cellular stress ***,the role of ATF3 in ZnO NP genotoxicity and cytotoxicity remains to be *** we reported that ZnO NP treatment dramatically induced the expression of ATF3 in human bronchial epithelial(HBE) cells,which was mediated by the nuclear factor erythroid 2-related factor 2(Nrf2).ATF3 was required for the repair of ZnO NP induced DNA damage as gamma foci number increased when endogenous ATF3 was ***,ATF3 also contributed to ZnO NP-induced cell *** study revealed that ATF3 interacted with the p53 protein and upregulated its expression under ZnO NP ***,our findings demonstrated ATF3 as an important regulator of epithelial homeostasis by promoting both DNA repair and the death of damaged cells under ZnO NP-induced genotoxic stress.

关键词： ZnO nanoparticle ATF3 DNA repair Apoptosis p53

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Inhibition of RNA polymerase Ⅲ transcription by Triptolide ...

华东六省一市生物化学与分子生物学会联盟2019年学术与...

作者： Xia Liang renxiang xie Jinfeng Su Bingqi Ye Saisai Wei Zhibing Liang Rongpan Bai Zhanghui Chen Zhongxiang Li Xiangwei Gao Medical Research Institute & Guangdong Women and Children's Disease Precision Diagnosis and Treatment Engineering Technology Research CenterBaoan Maternal and Child Health HospitalJinan University Department of Public Health Zhejiang University School of Medicine

Background:Upregulation of RNA polymerase(Pol) Ⅲ products,including tRNAs and 5 S rRNA,in tumor cells leads to enhanced protein synthesis and tumor formation,making it a potential target for cancer *** this study,we evaluated the inhibition of Pol Ⅲ transcription by triptolide and the anti-cancer effect of this drug in colorectal ***:The effect of triptolide on colorectal cancer development was assessed in colorectal cancer mouse models,3 D organoids,and cultured *** cancer cells were treated with *** Ⅲ transcription was measured by realtime quantitative polymerase chain reaction(PCR).The formation of TFⅢB,a multi-subunit transcription factor for Pol Ⅲ,was determined by chromatin immunoprecipitation(ChIP),co-immunoprecipitation(Co-IP),and fluorescence resonance energy transfer(FRET).Results:Triptolide reduced both tumor number and tumor size in adenomatous polyposis coli(Apc) mutated(ApcMin/+) mice as well as AOM/DSS-induced ***,triptolide effectively inhibited colorectal cancer cell proliferation,colonyformation,and organoid growth in vitro,which was associated with decreased Pol Ⅲ target ***,triptolide treatment blocked TBP/Brfl interaction,leading to the reduced formation of TFⅢB at the promoters of tRNAs and 5 S ***:Together,our data suggest that inhibition of Pol Ⅲ transcription with existing drugs such as triptolide provides a new avenue for developing novel therapies for colorectal cancer.

关键词： Triptolide Colorectal cancer RNA polymerase Ⅲ TFIIIB

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分布式数据采集系统的网络接口设计

微计算机信息 2006年第2S期22卷 138-140页

作者：谢仁祥钟睿李尚柏成都四川大学原子核科学技术研究所辐射物理及技术教育部重点实验 610064

讨论了分布式数据采集系统的实现方案。介绍了用TMS320C5409DSP芯片控制8019AS实现以太网通讯的硬件设计方案。实现了基于UDP协议的数据传输,并详细说明了方法和注意事项。

关键词：以太网 DSP 8019AS 分布式数据采集

Identification of highly efficacious PROTACs targeting BRD4 against acute myeloid leukemia:Design,synthesis,and biological evaluations

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Chinese Chemical Letters 2023年第6期34卷 426-431页

作者： Aiping Chen Yue Zhong Yunxiao Liu Zhancheng xie Hanyu Wu Wei Shi Wenlong Huang renxiang Tan Hai Qian College of Pharmacy Nanjing University of Chinese MedicineNanjing 210023China Center of Drug Discovery State Key Laboratory of Natural MedicinesChina Pharmaceutical UniversityNanjing 210009China Jiangsu Key Laboratory of Drug Discovery for Metabolic Disease China Pharmaceutical UniversityNanjing 210009China

The abnormal activation of BRD4 accelerates the progression of acute myeloid leukemia(AML),developing more precise therapeutics to intervene BRD4 promise to be an excellent opportunity to avoid current limitations of chemotherapy in ***,a range of small-molecule PROTACs with the privileged 8-methyl-pyrrolo[1,2-a]pyrazin-1(2H)-one scaffold were rationally designed,which harbored different carbon or ethylenedioxy chains to degrade BRD4 mediated by the E3 ubiquitin ligase *** them,the most potential B24 exhibited remarkable BRD4 degradation and excellent anti-proliferative activities in MV4-11 cells,with values of DC_(50)and IC_(50)for 0.75 nmol/L and 0.4 nmol/L,respectively,which were better than the BRD4 inhibitor(+)-***,this compound could time-dependently degrade the target protein in the BRD4-,CRBN-,and proteasome-dependent ***,B24 dramatically decreased the level of proto-oncogene c-Myc,and induced cell apoptosis by arresting the cell cycle in G0/G1 phase,down-regulating Bcl-2 and up-regulating Bax to amplify apoptotic *** proof-of-concept study also highlighted the feasibility of BRD4-based PROTACs as a more powerful strategy against AML.

关键词： Acute myeloid leukemia BRD4 PROTACs Protein degradation Apoptosis

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湿地公园水稻田冬季水鸟分布及其栖息地特征

中国城市林业 2024年第2期22卷 129-134页

作者：刘琳琳朱颖李欣谢冬王任翔冯育青苏州科技大学建筑与城市规划学院江苏苏州215011 江苏太湖湿地生态系统国家定位观测站江苏苏州215000 苏州市湿地保护管理站江苏苏州215000 南京林业大学生物与环境学院南京210037 苏州市林学会江苏苏州215011

稻田是我国人工湿地的重要组成部分。为探究冬季稻田管理模式对湿地水鸟多样性保护及栖息生境改善的重要意义,2017年12月至2018年2月及2018年11月,采用样带法对天福国家湿地公园冬季休耕蓄水区和传统稻田的水鸟种群进行调查,对比水鸟种... 详细信息

稻田是我国人工湿地的重要组成部分。为探究冬季稻田管理模式对湿地水鸟多样性保护及栖息生境改善的重要意义,2017年12月至2018年2月及2018年11月,采用样带法对天福国家湿地公园冬季休耕蓄水区和传统稻田的水鸟种群进行调查,对比水鸟种群及栖息地特征。结果表明:1)冬季休耕蓄水区记录4目7科25种水鸟,鸻鹬类占36%,鹭类占28%,雁鸭类占24%;传统稻田湿地共3目5科10种,其中鸻鹬类和鹭类分别占30%,无雁鸭类水鸟。2)冬季蓄水区水鸟多样性及物种丰富度较高,水鸟优势种群数量明显增多,Shannon-Wiener多样性指数(1.64)高于传统稻田(1.50),水鸟均匀度指数(0.84)低于传统稻田(1.37),相似性系数为0.51。3)冬季休耕蓄水区的水面面积、水位、食物、植被覆盖度是影响水鸟种数增多的重要因子,营巢地、停歇点也是影响冬季水鸟栖息的因素。因此,冬季稻田休耕蓄水管理的实施能够对生物多样性保护与提升发挥积极作用。

关键词：稻田冬季休耕蓄水区冬季水鸟栖息地天福国家湿地公园

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基于数据融合和改进MoCo的工业机器人抖动原因识别

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仪器仪表学报 2023年第7期44卷 112-120页

作者：陈仁祥谢文举徐向阳陈才张旭重庆交通大学交通工程应用机器人重庆市工程实验室重庆400074 重庆华数机器人有限公司重庆400714

实际工程中工业机器人受关节控制参数不佳易引起末端抖动,抖动原因识别有助于定位关节异常及优化控制。而工业机器人抖动原因识别存在周期信号冗余度高、抖动方向多及抖动状态样本标签缺失的问题,故提出基于数据融合和改进动量对比学习(... 详细信息

实际工程中工业机器人受关节控制参数不佳易引起末端抖动,抖动原因识别有助于定位关节异常及优化控制。而工业机器人抖动原因识别存在周期信号冗余度高、抖动方向多及抖动状态样本标签缺失的问题,故提出基于数据融合和改进动量对比学习(MoCo)的工业机器人抖动原因识别方法。首先,对工业机器人末端各传感器数据依次进行数据降维、数据扩充、水平拼接融合及降维,构建充足且全面反映抖动方向及状态信息的融合样本。其中,数据融合前降维可降低周期样本冗余度及提升样本融合效率,数据融合后降维可避免融合样本过长导致模型训练复杂度增加。其次,在MoCo前标记少量融合样本由正编码器分类通道输出监督信息,引导特征聚类。然后,改进对比学习策略,将正编码器提取的无标签融合数据特征与动量编码器保存的负样本特征的聚类中心进行对比,去除特征相似度最高的聚类中心以降低对比类别错误的假负样本干扰。并通过对称调换两个编码器的输入进行两次对比损失计算,完成编码器训练。最后,在编码器分类通道后添加Softmax分类器完成工业机器人抖动原因识别。实验结果表明,所提方法在不同工况的工业机器人抖动原因识别准确率均在90%以上,证明了该方法的有效性。

关键词：工业机器人抖动原因识别数据融合对比学习