检索结果-南通市图书馆

Colorimetric indicator based on zwitterionic anti-freezing hydrogel and alizarin for visual monitoring of salmon fillets freshness

在线全文

学校读者我要写书评

暂无评论

Journal of future Foods 2024年第2期4卷 135-141页

作者： Yanan Liu Huan Li Jian Chen Jiesi Feng renjie lian.linglin fu Yanbo Wang Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China Jinghai Group Co. Ltd.Rongcheng 264300China School of Food and Health Beijing Technology and Business UniversityBeijing 100048China

Indicating hydrogels have shown the advantages of being fast, sensitive, and real-time in demonstrating the freshness of aquatic products due to their noticeable color change. In this work, an anti-freezing hydrogel embedded with pH-sensitive alizarin was developed as an original colorimetric indicator for monitoring the corruption of aquatic products at low temperatures. The anti-freezing hydrogel was prepared from 2-carboxyethyl acrylate, [2-(methacryloyloxy)ethyl]-trimethylammonium chloride, and 2-hydroxyethyl methacrylate monomers with triethylene glycol dimethacrylate as cross-linking agent. The hydrogel showed excellent anti-freezing capability, which was further incorporated with the pH-sensitive dye alizarin to gain the indicating capability. When applied in the indication of the freshness of salmon under low-temperature storage, it was found that the color changes of the indicator corresponded with the total volatile basic nitrogen contents of salmon, the correlation coefficient of which was 0.959 6. These results indicated the application potential of the hydrogels as freshness indicators during low-temperature storage or cold-chain transportation.

关键词： Colorimetric indicator Alizarin Zwitterionic hydrogel Food freshness Visual monitoring

A novel AgNPs/MOF substrate-based SERS sensor for high-sensitive on-site detection of wheat gluten

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2024年第2期13卷 681-687页

作者： linglin fu Yanzhuo Du Jinru Zhou Huan Li Minzi Wang Yanbo Wang Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China Zhejiang Engineering Research Institute of Food&Drug Quality and Safety School of Management and E-BusinessZhejiang Gongshang UniversityHangzhou 310018China Zhejiang Dien Biotech Co. Ltd.Hangzhou 310030China

Gluten,known as the major allergen in wheat,has gained increasing concerns in industrialized countries,resulting in an urgent need for accurate,high-sensitive,and on-site detection of wheat gluten in complex food ***,we proposed a silver nanoparticles(AgNPs)/metal-organic framework(MOF)substrate-based surface-enhanced Raman scattering(SERS)sensor for the high-sensitive on-site detection of wheat *** detection occurred on the newly in-situ synthesized AgNPs/MOF-modified SERS substrate,providing an enhancement factor(EF)of 1.89×10^(5).Benefitting from the signal amplification function of AgNPs/MOF and the superiority of SERS,this sensor represented high sensitivity performance and a wide detection range from 1×10^(-15)mol/L to 2×10^(-6)mol/L with a detection limit of 1.16×10^(-16)mol/L,which allowed monitoring the trace of wheat gluten in complex food system without matrix *** reliable sandwich SERS sensor may provide a promising platform for high-sensitive,accurate,and on-site detection of allergens in the field of food safety.

关键词： Wheat allergy Gluten Surface-enhanced Raman scattering Metal-organic framework DNA recognition

Oral administration of Bacillus coagulans TQ-35 alleviates allergic responses in OVA-sensitive BALB/c mice

在线全文

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2024年第3期13卷 1246-1257页

作者： Yifan Wang Shanjun Chen Chong Wang Yi Zhang Hongliang Zeng linglin fu Yanbo Wang Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China College of Food Science Fujian Agriculture and Forestry UniversityFuzhou 350002China School of Food and Health Beijing Technology and Business UniversityBeijing 100048China

Bacillus coagulans has been extensively studied so far,but there has been a lack of research on its usage in *** this study,we designed to assess the effect of different concentrations of *** on food allergy in a BALB/c mouse model of ovalbumin(OVA)-induced food allergy and its effect on gut *** assessment of symptoms,specific immunoglobulin E(IgE),T-cell differentiation,and related gene expression levels in sensitized mice by assay indicated that high doses of oral *** could alleviate allergic *** with ***,in the high-dose group,significantly reduced IgE and IgG1 levels and modulated the balance of T helper type 1 cell(Th1)and Th2 and the expression of relevant genes in the spleen.16S rRNA analysis showed that probiotics improved the structure of the microbiota,in particular by boosting the percentage of Clostridia,Bacteroides vulgatus and Enterococcus faecium,and by increasing the abundance of microbial species,thereby modulating the immune ***,this study can provide insights into the practical application of *** doses to alleviate OVA allergy.

关键词： Bacillus coagulans TQ-35 Food allergy Ovalbumin(OVA) Gut microbiota

Site-directed mutagenesis of bifunctional riboflavin kinase/FMN adenylyltransferase via CRISPR/Cas9 to enhance riboflavin production

在线全文

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2024年第3期9卷 503-512页

作者： Bing fu Meng Chen Xianfeng Bao Jiajie Lu Zhiwen Zhu fuyao Guan Chuyang Yan Peize Wang linglin fu Ping Yu College of Food Science and Biotechnology Zhejiang Gongshang University149 Jiaogong RoadHangzhouZhejiang Province310035People’s Republic of China College of Forestry Science and Technology Lishui Vocational and Technical College357 Zhongshan Street NorthLishuiZhejiang Province323000People’s Republic of China Lishui Institute for Quality Inspection and Testing 395 Zhongshan StreetLishuiZhejiang Province323000People’s Republic of China

Vitamin B_(2)is an essential water-soluble *** most prokaryotes,a bifunctional enzyme called FAD synthase catalyzes the successive conversion of riboflavin to FMN and *** this study,the plasmid pNEW-AZ containing six key genes for the riboflavin synthesis was transformed into strain R2 with the deleted FMN riboswitch,yielding strain *** R5 strain could produce 540.23±5.40 mg/L riboflavin,which was 10.61%higher than the R4 strain containing plasmids pET-AE and pAC-Z harboring six key *** further enhance the production of riboflavin,homology matching and molecular docking were performed to identify key amino acid residues of FAD *** point mutation sites were *** comparing riboflavin kinase activity,mutations of T203D and N210D,which respectively decreased by 29.90%and 89.32%compared to wild-type FAD synthase,were selected for CRISPR/Cas9 gene editing of the genome,generating engineered strains R203 and ***-AZ was transformed into R203,generating R6.R6 produced 657.38±47.48 mg/L riboflavin,a 21.69%increase compared to *** study contributes to the high production of riboflavin in recombinant *** BL21.

关键词： Vitamin B_(2) FAD synthase Escherichia coli BL21 Riboflavin kinase CRISPR/Cas9

Advance and Application of Single‑cell Transcriptomics in Auditory Research

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neuroscience Bulletin 2024年第7期40卷 963-980页

作者： Xiangyu Ma Jiamin Guo Mengyao Tian Yaoyang fu Pei Jiang Yuan Zhang renjie Chai State Key Laboratory of Digital Medical Engineering Department of Otolaryngology Head and Neck SurgeryZhongda HospitalSchool of Life Sciences and TechnologyAdvanced Institute for Life and HealthJiangsu Province High‑Tech Key Laboratory for Bio‑Medical ResearchSoutheast UniversityNanjing 210096China Department of Psychiatry Affiliated Hangzhou First People’s HospitalZhejiang University school of MedicineHangzhou 310030China Department of Otolaryngology Head and Neck Surgery Affiliated Drum Tower Hospital of Nanjing University Medical SchoolJiangsu Provincial Key Medical Discipline(Laboratory)NanjingChina Research Institute of Otolaryngology Nanjing 210008China Department of Otolaryngology Head and Neck Surgery Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610072China Co‑Innovation Center of Neuroregeneration Nantong UniversityNantong 226001China Institute for Stem Cell and Regeneration Chinese Academy of ScienceBeijing 101408China Beijing Key Laboratory of Neural Regeneration and Repair Capital Medical UniversityBeijing 100069China

Hearing loss and deafness,as a worldwide disability disease,have been troubling human ***,the auditory organ of the inner ear is highly heterogeneous and has a very limited number of cells,which are largely uncharacterized in ***,with the development and utilization of single-cell RNA sequencing(scRNA-seq),researchers have been able to unveil the complex and sophisticated biological mechanisms of various types of cells in the auditory organ at the single-cell level and address the challenges of cellular heterogeneity that are not resolved through by conventional bulk RNA sequencing(bulk RNAseq).Herein,we reviewed the application of scRNA-seq technology in auditory research,with the aim of providing a reference for the development of auditory organs,the pathogenesis of hearing loss,and regenerative *** about spatial transcriptomic scRNA-seq,single-cell based genome,and Live-seq technology will also be discussed.

关键词： Single-cell RNA sequencing Inner ear Cochlear Auditory sensory epithelium Spiral ganglion neuron

Comparison of immune responses and intestinal flora in epicutaneously sensitized BALB/c or C57BL/6 mouse models of food allergy

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2024年第2期13卷 668-680页

作者： Gang Yu Yuhao Jiang Shuifeng Zhang Pengpeng Liu Shunyu Wang Huadong Sheng Yanbo Wang Qiaozhi Zhang linglin fu Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China Zhejiang Fangyuan Test Group Co. Ltd.Hangzhou 310018China Key Laboratory of Biosafety Detection for Zhejiang Market Regulation Hangzhou 310018China Zhejiang Liziyuan Food Co. LtdJinhua 321031China

Cutaneous exposure to food allergens through a disrupted skin barrier is recognized as an important cause of food allergy,and the cutaneous sensitized mouse model has been established to investigate relevant allergic ***,the role of different genetic backgrounds of mice on immune responses to food allergens upon epicutaneous sensitization is largely *** this study,two strains of mice,i.e.,the BALB/c and C57BL/6 mice,were epicutaneously sensitized with ovalbumin on atopic dermatitis(AD)-like skin lesions,followed by intragastric challenge to induce IgE-mediated food *** outcomes were measured as clinical signs,specific antibodies and cytokines,and immune cell subpopulations,as well as changes in intestinal barrier function and gut *** showed that both strains of mice exhibited typical food-allergic symptoms with a Th2-skewed *** C57BL/6 mice,rather than the BALB/c mice,were fitter for establishing an epicutaneously sensitized model of food allergy since a stronger Th2-biased response and severer disruptions in the intestinal barrier and gut homeostasis were *** study provides knowledge for selecting an appropriate mouse model to study food-allergic responses associated with AD-like skin lesions and highlights the role of genetic variations in the immune mechanism underlying pathogenesis of food allergy.

关键词： Food allergy Mouse models Epicutaneous sensitization Th2 response Gut microbiota

Comprehensive analysis of advanced glycation end-products in commonly consumed foods:presenting a database for dietary AGEs and associated exposure assessment

在线全文

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2024年第4期13卷 1917-1928页

作者： Qiaozhi Zhang Huatao Li Ruixing Zheng Lili Cao Shufen Zhang Shuifeng Zhang Huadong Sheng Yuhao Jiang Yanbo Wang linglin fu Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China Ningbo Academy of Product and Food Quality Inspection(Ningbo Fibre Inspection Institute) Ningbo 315048China National Pre-packaged Food Quality Supervision and Inspection Center(Zhejiang) Zhejiang Fangyuan Test Group Co.Ltd.Hangzhou 310018China

Advanced glycation end-products(AGEs)are a group of heterogeneous compounds formed in heatprocessed foods and are proven to be detrimental to human ***,there is no comprehensive database for AGEs in foods that covers the entire range of food categories,which limits the accurate risk assessment of dietary AGEs in human *** this study,we first established an isotope dilution UHPLCQq Q-MS/MS-based method for simultaneous quantification of 10 major AGEs in *** contents of these AGEs were detected in 334 foods covering all main groups consumed in Western and Chinese populations.Nε-Carboxymethyllysine,methylglyoxal-derived hydroimidazolone isomers,and glyoxal-derived hydroimidazolone-1 are predominant AGEs found in most *** amounts of AGEs were high in processed nuts,bakery products,and certain types of cereals and meats(>150 mg/kg),while low in dairy products,vegetables,fruits,and beverages(<40 mg/kg).Assessment of estimated daily intake implied that the contribution of food groups to daily AGE intake varied a lot under different eating patterns,and selection of high-AGE foods leads to up to a 2.7-fold higher intake of AGEs through daily *** presented AGE database allows accurate assessment of dietary exposure to these glycotoxins to explore their physiological impacts on human health.

关键词： Advanced glycation end-products Maillard reaction Processed foods Dietary database Exposure assessment

Rice bran polysaccharide ameliorates ovalbumin-induced allergy and improves gut microbiota composition

在线全文

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2024年

作者： Bingjie Chen Yongjin Qiao Chong Wang Xiao Wang Yi Zhang linglin fu School of Food Science and Biotechnology， Zhejiang Gongshang University Institute of Crop Breeding and Cultivation， Shanghai Academy of Agricultural Science

This study is the first investigation of the effects of polysaccharide extracted from rice bran (RBP) on ovalbumin (OVA)-induced allergy and the gut microbiota， which hasn't been reported previously. RBP in the medium-dose was found to significantly reduce the symptoms of food allergy (FA) in mice， lower the levels of histamine and allergen-specific (IgE)， and attenuate intestinal inflammation. further studies demonstrated that RBP decreased allergy-associated responses in intestinal epithelial cells and Th2 cells while regulating Th1/Th2 differentiation to reach a dynamic equilibrium and downregulating Th2 polarization caused by allergies. Short-chain fatty acid (SCFAs) content and 16S rRNA sequencing revealed that RBP enhanced the abundance and diversity of the gut microflora and raised the content of SCFAs. These results suggest that RBP may mitigate FA by modulating the gut microbiota. This study revealed the effective use of rice bran and served as a basis for the development of innovative functional foods with anti-allergic properties.

关键词：

Dietary polyphenols reduced the allergenicity ofβ-lactoglobulin via non-covalent interactions:a study on the structure-allergenicity relationship

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2024年第5期13卷 2617-2628页

作者： Chi Zhang Qiaozhi Zhang Huatao Li Zhouzhou Cheng Shiyu Fan Hujun Xie Zhongshan Gao Yan Zhang linglin fu School of Food Science and Biotechnology Zhejiang Gongshang UniversityHangzhou 310018China Allergy Research Center Zhejiang UniversityHangzhou 310018China Hebei Food Inspection and Research Institute Hebei Food Safety Key LaboratoryKey Laboratory of Special Food Supervision Technology for State Market RegulationHebei Engineering Research Center for Special Food Safety and HealthShijiazhuang 050227China

Studies showed that complexation of polyphenols with milk allergens reduced their immunogenic ***,the relationship between structures of polyphenols and their hypoallergenic effects on milk allergens in association with physiological and conformational changes of the complexes remain *** this study,polyphenols from eight botanical sources were extracted to prepare non-covalent complexes withβ-lactoglobulin(β-LG),a major allergen in *** dominant phenolic compounds bound toβ-LG with a diminished allergenicity were identified to investigate their respective role on the structural and allergenic properties ofβ-*** from Vaccinium fruits and black soybeans were found to have great inhibitory effects on the IgE-and IgG-binding abilities ofβ-*** the fourteen structure-related phenolic compounds,flavonoids and tannins with larger MWs and multi-hydroxyl substituents,notably rutin,EGCG,and ellagitannins were more potent to elicit changes on the conformational structures ofβ-LG to decrease the allergenicity of complexedβ-*** analysis further demonstrated that a destabilized secondary structure and protein depolymerization caused by polyphenol-binding were closely related to the allergenicity property of formed *** study provides insights into the understanding of structure-allergenicity relationship ofβ-LG-polyphenol interactions and would benefit the development of polyphenol-fortified matrices with hypoallergenic potential.

关键词： Cow’s milk allergy β-Lactoglobulin Protein-polyphenol interaction Allergenicity Conformational structure