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Journal of Oceanology and limnology 2024年第1期42卷 263-276页

作者： Ruozhen GENG Zhongshi HE Kaihui GAO Peng XIAO He ZHANG Si CHEN Hua li Gongliang YU renhui li College of life and Environmental Sciences Wenzhou UniversityWenzhou 325035China Key Laboratory of Algal Biology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of Chinese Academy of Sciences Beijing 100049China Hubei Institution of Land Surveying and Mapping Wuhan 430072China

A new filamentous cyanobacterial strain(CHAB 4127)was successfully isolated from the algal mat during the field investigation of the open channel from Luanhe River to Tianjin,China.The polyphasic approach combining morphological,ultrastructural,ecological,and molecular features was used to characterize this studied strain.The strain is morphologically similar to the Microcoleus-like cyanobacterial taxa under light microscopy,and the radial arrangement of thylakoids is also consistent with that of Microcoleus-like groups.The phylogenetic position of CHAB 4127 based on 16S rRNA gene sequences,is shown to be clearly clustered into an independent clade with the newly established genus Microcoleusiopsis.The maximum similarity of 16S r RNA gene of the studied strain with other existing related cyanobacterial taxa is 93.97%,and the ITS secondary structures is also obviously different from other members of Microcoleaceae.Based on all the above evidence,we proposed the establishment of this novel cyanobacterial genus as Neomicrocoleus,with its type species,Neomicrocoleus tianjinensis.

关键词： Neomicrocoleus tianjinensis morphology 16S rRNA taxonomy polyphasic approach

Description of two new species of Pseudoaliinostoc(Nostocales,Cyanobacteria)from China based on the polyphasic approach

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Journal of Oceanology and limnology 2022年第3期40卷 1233-1244页

作者： Fangfang CAI Gongliang YU renhui li Hubei Key Laboratory of Animal Nutrition and Feed Science Hubei Collaborative Innovation Center for Animal Nutrition and Feed SafetyWuhan Polytechnic UniversityWuhan 430023China Key Laboratory of Algal Biology State Key Laboratory of Freshwater Ecology and Biotechnology of ChinaInstitute of HydrobiologyChinese Academy of SciencesWuhan 430072China School of life and Environmental Sciences Wenzhou UniversityWenzhou 325035China

Two cyanobacterial strains CHAB5870 and CHAB5871 morphologically identifi ed as Nostoc-like species were isolated from different habitats in China,and they were phylogenetically and taxonomically characterized based on a polyphasic approach combining morphological,ecological,and molecular data.In the 16S rRNA gene phylogeny inferred using maximum likelihood,maximum-parismony,and bayesian inference methods,these two strains clustered within the Pseudoaliinostoc clade.The 16S rRNA gene sequences of these two strains displayed≥95.5%and≤98%similarity to Pseudoaliinostoc species,which indicated them to represent new species of the genus Pseudoaliinostoc.Furthermore,the unique pattern of D1-D1′and Box-B helix of the 16S–23S rRNA internal transcribed spacer(ITS)secondary structure also revealed that two strains represented novel species.These results supported the establishment of two new Pseudoaliinostoc species with the name P.jiangxiense sp.***.and P.yunnanense sp.nov.

关键词： 16S rRNA gene 16S–23S ITS new species morphology polyphasic approach taxonomy

Succession of Aphanizomenon flos-aquae and Microcystis aeruginosa in direct co-culture experiments at different temperatures and biomasses

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Journal of Oceanology and limnology 2022年第5期40卷 1819-1828页

作者： Qianzhi WEN Peng XIAO Hua li Wenke li Gongliang YU renhui li Key Laboratory of Algal Biology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of Chinese Academy of Sciences Beijing 100049China College of life and Environmental Sciences Wenzhou UniversityWenzhou 325035China

Cyanobacterial blooms have become a serious global environmental issue due to their potential risk for releasing detrimental secondary metabolites into aquatic ecosystems,posing a great threat to water quality management for public health authorities.Aphanizomenon,a common filamentous cyanobacterial genus belonging to Nostocales,is under particular concern because its several members are able to form harmful blooms.Furthermore,succession of bloom between A.flos-aquae and Microcystis occurs in many natural lakes.To evaluate the competitiveness of A.flos-aquae vs.M.aeruginosa,two sets of experiments at different ratios of biomass at 15℃and 25℃were conducted.Results show that at 15℃,the two species were able to coexist,and A.flos-aquae showed a specific higher growth rate,and its growth was promoted by the presence of M.aeruginosa.At 25℃,the growth of A.flos-aquae was inhibited by the biomass of M.aeruginosa,and M.aeruginosa suppressed A.flos-aquae in competition.Additionally,the vegetative cell size of A.flos-aquae was significantly influenced by the co-culture with M.aeruginosa,whereas the filament length of A.flos-aquae was not significantly affected.This study confirms that temperature is the dominating factor on the succession of A.flos-aquae and M.aeruginosa of a different biomass.

关键词： cyanobacterial bloom Aphanizomenon flos-aquae Microcystis aeruginosa succession temperature biomass

Cyanobacterial blooms in China:ecology,toxicity,and treatment

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Journal of Oceanology and limnology 2022年第5期40卷 1693-1695页

作者： renhui li Xiaoli SHI Nanqin GAN Junyi ZHANG Xuechu CHEN National and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution Wenzhou UniversityWenzhou 325035China State Key Laboratory of Lake Science and Environment Nanjing Institute of Geography and LimnologyChinese Academy of SciencesNanjing 210008China State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China Wuxi Environmental Monitoring Center Wuxi 214121China School of Ecological and Environmental Sciences East China Normal UniversityShanghai 200241China

Cyanobacterial blooms are one of the major threats to the health of aquatic ecosystems,and are increasing globally due to the synergistic eff ects of human activities and global climate change.The 7^(th) National Cyanobacteria Bloom Forum was successfully held in May 28-30,2021 in Guiyang,Guizhou Province,China.The forum established a platform for exchanging views on the concerns of cyanobacterial blooms from Chinese researchers,lake managers,and treatment engineers.This special issue,“Cyanobacterial blooms in China:ecology,toxicity,and treatment”in Journal of Oceanography and limnology,presents a collection of 14 papers on the physiological features and the infl uence on their concurrent aquatic biology of diff erent cyanobacterial groups.

关键词： treatment Guizhou globally

Competitiveness of alga Microcystis aeruginosa co-cultivated with cyanobacterium Raphidiopsis raciborskii confirms its dominating position

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Journal of Oceanology and limnology 2022年第5期40卷 1804-1818页

作者： Zengling MA Xiaoqiao ZHANG renhui li Min WANG Wenli QIN He ZHANG Gang li Henguo YU Chuanjun DAI Min ZHAO Zhejiang Provincial Key Laboratory for Subtropical Water Environment and Marine Biological Resources Protection Wenzhou UniversityWenzhou 325035China National and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution Wenzhou UniversityWenzhou 325035China Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China

Microcystis aeruginosa has always been regarded as the main culprit of cyanobacterial blooms in freshwater.However,in recent years,Raphidiopsis raciborskii has gradually replaced M.aeruginosa as the culprit of cyanobacterial blooms in some tropical and subtropical shallow lakes.To reveal which one plays a more dominant role,interactions between cylindrospermospin(CYN)-producing R.raciborskii and microcystins(MCs)-producing or non-MCs-producing M.aeruginosa strains were studied using bialgal cultures at different initial ratios of biomasses of the two species at 25℃.During the co-cultivation,the M.aeruginosa strains inhibited the growth and heterocyst formation of R.raciborskii filaments,and thus occupied a dominant position during the co-cultivation regardless of the initial biomass ratios in the cultures.In addition,the MCs-producing M aeruginosa strain contributed to a higher portion of the total biomass and exerted a stronger inhibitory effect on R.raciborskii compared with the non-MCs-producing strain.However,the growth of both MCs-producing and non-MCs-producing M.aeruginosa strains was stimulated by R.raciborskii in the co-cultures compared with M.aeruginosa monoculture,indicating that M.aeruginosa could outcompete R.raciborskii if given enough time,enabling it to develop into the dominant species even in very low initial concentration.To our best knowledge,this is the first report on the loss of heterocyst formation by a species of cyanobacteria that resulted from interactions between two different species of cyanobacteria.These findings indicate that it is difficult for R.raciborskii to replace the dominant position of M.aeruginosa under the experimental environmental condition,and the allelopathic effects of M.aeruginosa on R.raciborskii could significantly contribute to the success of M.aeruginosa.

关键词： competition growth heterocyst Microcystis aeruginosa morphology Raphidiopsis raciborskii

Combining single-cell RNA sequencing with spatial transcriptome analysis reveals dynamic molecular maps of cambium differentiation in the primary and secondary growth of trees

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Plant Communications 2023年第5期4卷 46-62页

作者： renhui li Zhifeng Wang Jia-Wei Wang Laigeng li National Key Laboratory of Plant Molecular Genetics and CAS Center for Excellence in Molecular Plant Sciences Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China University of the Chinese Academy of Sciences Beijing 100049China School of life Science and Technology ShanghaiTech UniversityShanghai 201210China

Primary and secondary growth of the tree stem are responsible for corresponding increases in trunk height and diameter.However,our molecular understanding of the biological processes that underlie these two types of growth is incomplete.In this study,we used single-cell RNA sequencing and spatial transcriptome sequencing to reveal the transcriptional landscapes of primary and secondary growth tissues in the Populus stem.Comparison between the cell atlas and differentiation trajectory of primary and secondary growth revealed different regulatory networks involved in cell differentiation from cambium to xylem precursors and phloem precursors.These regulatory networks may be controlled by auxin accumulation and distribution.Analysis of cell differentiation trajectories suggested that vessel and fiber development followed a sequential pattern of progressive transcriptional regulation.This research provides new insights into the processes of cell identity and differentiation that occur throughout primary and secondary growth of tree stems,increasing our understanding of the cellular differentiation dynamics that occur during stemgrowth in trees.

关键词： scRNA-seq spatial transcriptome primary growth secondary growth secondary cell wall SCW Populus

Tidal water exchanges can shape the phytoplankton community structure and reduce the risk of harmful cyanobacterial blooms in a semi-closed lake

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Journal of Oceanology and limnology 2022年第5期40卷 1868-1880页

作者： Wenxu ZHENG renhui li Wenli QIN Binbin CHEN Min WANG Wanchun GUAN Xiaoling ZHANG Qiao YANG Min ZHAO Zengling MA Zhejiang Provincial Key Laboratory for Subtropical Water Environment and Marine Biological Resources Protection Wenzhou UniversityWenzhou 325035China National and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution Wenzhou UniversityWenzhou 325035China Department of Marine Biotechnology School of Laboratory Medicine and Life ScienceWenzhou Medical UniversityWenzhou 325035China Marine Science and Technology College Zhejiang Ocean UniversityZhoushan 316022China School of Petrochemical Engineering and Environment Zhejiang Ocean UniversityZhoushan 316022China

For estuaries,inland lakes play a vital role in the ecological balance under the impact of tide s.The effect of tides-induced water exchange on phytoplankton community in a semi-closed lake was studied and compared with that of an adjacent closed lake in the Oujiang River mouth in Zhejiang,East China Sea,from June 29,2020 to June 14,2021.Results show that the dominant species,abundance,dominance,and diversity of the phytoplankton species between the two lakes were significantly different.In the closed lake,cyanobacteria were the dominant species during the study period.However.in the semi-closed lake,the diversification of the dominant species was greater,and some species of diatoms and green algae became dominant.The average phytoplankton abundance in the closed lake was 6 times of that in the semi-closed lake.The average dominance of cyanobacteria in the closed lake was 0.96,and those in the semi-closed lake and the Oujiang River were 0.51 and 0.22,respectively.Cyanobacterial blooms occurred throughout the study time in the closed lake but not in the semi-closed one.Furthermore,the species diversity richness of the phytoplankton in the semi-closed lake was higher than that of the closed one,and the phytoplankton community between the closed lake and semi-closed lake could be divided into distinctly different groups based on non-metic multidimensional scaling analysis(NMDS)and analysis of similarities(ANOSIM)analysis.The salinity of the water was significantly greater and the transparency significantly smaller in the semi-closed lake than those in the closed lake.Therefore,water exchange driven by local tidal movement increased salinity and decreased transparency of water,which consequently shaped the community structures of different phytoplankton and reduced the risk of a cyanobacterial bloom outbreak in the semi-closed lake.

关键词： estuary harmful cyanobacterial blooms phytoplankton community water exchange tidal movement environmental parameters

Molecular characterization and toxin quantification of Microcystis panniformis: A microcystin producer in Lake Taihu,China

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Journal of Environmental Sciences 2019年第2期31卷 359-367页

作者： Yilu Gu Xiaofeng Gao Yongguang Jiang Xin Peng renhui li Key laboratory of Algal Biology Institute of HydrobiologyChinese Academy of Sciences Department of Urban Engineering The University of Tokyo School of Environmental Studies China University of Geosciences

Microcystis panniformis is a bloom forming species with flat panniform-like colonies. This species was recently found in Lake Taihu, China. To specifically characterize M. panniformis based on isolated strains, morphological examination on colonial transition and genetic examination are needed. Three M. panniformis strains isolated from a water bloom sample in Lake Taihu were characterized by molecular analysis and toxin quantification. Phylogenetic analysis based on both 16S rRNA gene and internal transcribed spacer(ITS) between 16S and 23S rRNA genes were performed and compared to facilitate easy identification of the species.Relatively high similarities(98%–99%) were shown in 16S rDNA sequences between the strains of M. panniformis and those of other Microcystis species, whereas the similarities for ITS sequences were 88%–95%. In the phylogenetic tree based on the 16S rDNA sequences, the M. panniformis and M. aeruginosa strains were intermixed together with no clear division,whereas all of the M. panniformis strains were clustered together in a single clade based on the ITS sequences based phylogenyetic tree. The mcyE gene was detected in all three strains, and microcystin was determined by high-performance liquid chromatography. The molecular detection and toxin production of M. panniformis strains are of great significance for the environmental risk assessment of Microcystis blooms.

关键词： Microcystis panniformis Blooms ITS of 16S-23S rRNA mcyE Microcystin Taihu

Responses of Synechocystis sp. PCC 6803 (cyanobacterium) photosystem Ⅱ to pyrene stress

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Journal of Environmental Sciences 2010年第7期22卷 1091-1095页

作者： Jihai Shao Gongliang Yu Zhongxing Wu Xin Peng renhui li Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate School of Chinese Academy of Sciences Beijing 100039 China College of Resources and Environment Hunan Agricultural University Changsha 410128 China

In order to explore the mechanism of acute toxicity for pyrene to cyanobacterial organisms, the responses of Synechocystis sp. PCC 6803 photosystem Ⅱ （PS Ⅱ） under pyrene stress were studied. The results showed there was no significant difference about the oxygen evolution under 0.125 mg/L pyrene stress when compared with control, but it was significantly lower than control at 0.625 mg/L pyrene. Polyphasic chlorophyll-a fluorescence transients in cells of Synechocystis sp. PCC 6803 exhibited a typical increase including O, J, I, and P phases. Fluorescence yield at phases J, I and P declined slightly at 0.125 and 0.625 mg/L pyrene, and significantly lower than control at 3.125 mg/L. According to the parameters deviated from JIP-test, no modification was induced by pyrene both at the donor side and at the acceptor side of PS Ⅱ, and the reaction centre of PS Ⅱ is the primary damaging target. Based on the expressing of four key genes （psbA, psbB, psbC and psbO） of PS Ⅱ, only psbA showed significant difference at 3.125 mg/L pyrene when compared with control.

关键词： pyrene Synechocystis toxicity photosystem Ⅱ

First report of microcystin production in Microcystis smithii Komrek and Anagnostidis(Cyanobacteria) from a water bloom in Eastern China

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Journal of Environmental Sciences 2011年第1期23卷 102-107页

作者： Yang liu Wenhua Tan Xingqiang Wu Zhongxing Wu Gongliang Yu renhui li Key Laboratory of Aquatic Biodiversity and Conservation Biology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate University of Chinese Academy of Sciences Beijing 100049 China

A water bloom sample collected from Lake Dishui in Shanghai was characterized. The morphological identification showed that Micorcystis wesenbergii and Micorcystis smithii were the main component of the bloom. Five strains of M. smithii were successfully isolated. Their 16S rRNA gene sequences based phylogenetic tree showed that the five strains of M. smithii intermixed with strains of other morphospecies in Microcystis. A fragment of mcy gene encoding for microcystin synthetase was detected in one of the five M. smithii strains （CHAB 2183）, indicating its potential of microcystin production. High performance liquid chromatography analysis confirmed M. smithii CHAB 2183 to produce microcystin-RR as 1550 μg per gram dry weight cells. The present investigation, for the first time, reported the isolated strains of M. smithii and microcystin production from M. smithii.

关键词： Cyanobacteria Microcystis smithii microcystin-RR mcyA 16S rRNA gene