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The Stripe Rust Resistance Gene Yr10 Encodes an Evolutionary-Conserved and Unique CC-NBS-LRR Sequence in Wheat

Molecular Plant 2014年第12期7卷 1740-1755页

作者： Wei Liu Michele Frick rene huel Cory L. Nykiforuk Xiaomin Wang Denis A. Gaudet Francois Eudes Robert L. Conner Alan Kuzyk Zhensheng Kang Andre Laroche State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F University YanglingShaanxi 712100 RR. China Agriculture and Agri-Food Canada Lethbridge Research Centre 5403 1st Avenue South Lethbridge Alberta T1J 4B1 Canada Current address: The International Commission on Missing Persons Alipasina 45A Sarajevo 71000 Bosnia and Herzegovina Current address: Emergent BioSolutions 155 Innovation Drive Winnipeg MB R3T 5Y3 Canada Current address: Agriculture and Agri-Food Canada Morden Research Centre Unit 100-101 Route 100 Morden MB R6M 1Y5 Canada Retired from Agriculture and Agri-Food Canada

The first seedling or all-stage resistance （R） R gene against stripe rust isolated from Moro wheat （Triticum aes- tivum L.） using a map-based cloning approach was identified as Yr10. Clone 4B of this gene encodes a highly evolutionary- conserved and unique CC-NBS-LRR sequence. Clone 4E, a homolog of Yr10, but lacking transcription start site （TSS） and putative TATA-box and CAAT-box, is likely a non-expressed pseudogene. Clones 4B and 4E are 84% identical and divergent in the intron and the LRR domain. Gene silencing and transgenesis were used in conjunction with inoculation with differen- tially avirulent and virulent stripe rust strains to demonstrate Yr10 functionality. The Yr10 CC-NBS-LRR sequence is unique among known CC-NBS-LRR R genes in wheat but highly conserved homologs （E = 0.0） were identified in Aegilops tauschii and other monocots including Hordeum vulgare and Brachypodium distachyon. Related sequences were also identified in genomic databases of maize, rice, and in sorghum. This is the first report of a CC-NBS-LRR resistance gene in plants with limited homologies in its native host, but with numerous homologous R genes in related monocots that are either host or non-hosts for stripe rust. These results represent a unique example of gene evolution and dispersion across species.

关键词： seedling or all-stage stripe rust resistance gene functionality transgenesis gene silencing homolog sequences coiled-coil region nucleot de-b nding site leucine-rich repeat domain.

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A highly conserved and unique CC-NBS-LRR sequence in grasses encodes the Yr10 stripe rust resistance gene

A highly conserved and unique CC-NBS-LRR sequence in grasses...

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第十三届全国植物基因组学大会

作者： Michele Frick rene huel Cory L.Nykiforuk Denis A.Gaudet Francois Eudes Robert L.Conner Alan Kuzyk andre Laroche Agriculture and Agrifood Canada Lethbridge Research CentreAlberta OCanada Agriculture and Agrifood Canada Morden Research CentreMorden OCanada

Attempts to clone Yr10 by map-based cloning led to the identification of two linked genomic clones encoding highly conserved and unique CC-NBS-LRR sequences. Clone 4B Correspondings to an expressed single copy transcript whereas 4E is a *** between the sequences showed some divergence in the intron and LRR sequences and they appear to form a two-member gene family.

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MODELING SPATIAL AND TEMPORAL VARIATION OF A POLLUTANT IN WATER ENVIRONMENT USING THE CUBIC SPLINE SCHEME

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Journal of Hydrodynamics 2006年第6期18卷 688-697页

作者： YAN Min KAHAWITA rene College of Architecture and Civil Engineering Zhejiang University of Technology Hangzhou 310014 China Department of Civil Geological and Mining Engineering Ecole Polytechnique de Montréal Université deMontr6al Quebec H3C 3A7 Canada Department of Civil Geological and Mining Engineering Ecole Polytechnique de Montréal Université de Montréal Quebec H3C 3A7 Canada

The cubic spline integration scheme has been successfully applied for the first time to obtain solutions for the two-dimensional shallow water wave and transport equations. Its principal advantages are its high accuracy, low computational cost, and flexibility of application. The study quantitatively analyzed and predicted the variation in time and space of a solid pollutant in water environment. The infiltration, adsorption, solubility, advection, and diffusion related factors at different spatial and temporal scales were taken into consideration in the model. Experiments were conducted in this study to examine the capabilities of the cubic spline scheme, The correspondence between the numerical results and the experimental data was fair, The results were also compared with the numerical results obtained using finite difference schemes with good agreement.

关键词： numerical model cubic spline scheme,hydraulics environment pollution

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Hepatic organ protection:From basic science to clinical practice

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World Journal of Gastroenterology 2010年第48期16卷 6044-6045页

作者： rene Schmidt Department of Anesthesiology and Critical Care Medicine Freiburg University Medical Center

Hepatic ischemia and reperfusion (I/R) injury during liver surgery is still the main cause of postoperative liver failure and the subsequent rise of mortality in these patients. During the last few years, a multitude of underlying mechanisms have been extensively characterized and many different protective approaches have been evaluated under experimental conditions. Some of them have already found their way into small sized clinical trials. In this Topic Highlight series of articles, we present recent insights into promising protective concepts including the regulation and optimization of hepatic blood flow, molecular mechanisms of preconditioning and pharmacological approaches with the aim of limiting hepatic I/R injury. Leading international experts present the latest experimental evidence in their fields stressing clinically relevant ideas, which are now on the edge of entering clinical practice.

关键词： Heme oxygenase-1 Hepatectomy Hepatic organ protection Ischemia/reperfusion injury Liver blood flow Liver transplantation Preconditioning

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Do people communicate about their whereabouts? Investigating the relation between user-generated text messages and Foursquare check-in places

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Geo-Spatial Information Science 2018年第3期21卷 159-172页

作者： Ming Li rene Westerholt Alexander Zipf Institute of Geography Heidelberg UniversityHeidelbergGermany

The social functionality of places(***,restaurant)partly determines human behaviors and reflects a region’s functional *** descriptions of places are thus valuable to a range of studies of humans and geographic *** their potential impacts on human verbalization behaviors,one possibility is to link the functions of places to verbal representations such as users’postings in location-based social networks(LBSNs).In this study,we examine whether the heterogeneous user-generated text snippets found in LBSNs reliably reflect the semantic concepts attached with check-in *** investigate Foursquare because its available categorization hierarchy provides rich a-priori semantic knowledge about its check-in places,which enables a reliable verification of the semantic concepts identified fromuser-generated text snippets.A latent semantic analysis is conducted on a large Foursquare check-in *** results confirm that attached text messages can represent semantic concepts by demonstrating their large correspondence to the official Foursquare venue *** further elaborate the representativeness of text messages,this work also performs an investigation on the textual terms to quantify their abilities of representing semantic concepts(i.e.,representativeness),and another investigation on semantic concepts to quantify how well they can be represented by text messages(i.e.,representability).The results shed light on featured terms with strong locational characteristics,as well as on distinctive semantic concepts with potentially strong impacts on human verbalizations.

关键词： Text mining latent semantic analysis semantic concepts Foursquare location-based social network(LBSN)

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Taking advantage of drug resistance, a new approach in the war on cancer

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Frontiers of Medicine 2018年第4期12卷 490-495页

作者： Liqin Wang rene Bernards Division of Molecular Carcinogenesis Oncode Institute The Netherlands Cancer Institute Plesmanlaan 121 1066 CX Amsterdam The Netherlands

Identification of the driver mutations in cancer has resulted in the development of a new category of molecularly targeted anti-cancer drugs. However, as was the case with conventional chemotherapies, the effectiveness of these drugs is limited by the emergence of drug-resistant variants. While most cancer therapies are given in combinations that are designed to avoid drug resistance, we discuss here therapeutic approaches that take advantage of the changes in cancer cells that arise upon development of drug resistance. This approach is based on notion that drug resistance comes at a fitness cost to the cancer cell that can be exploited for therapeutic benefit. We discuss the development of sequential drug therapies in which the first therapy is not given with curative intent, but to induce a major new sensitivity that can be targeted with a second drug that selectively targets the acquired vulnerability. This concept of collateral sensitivity has hitherto not been used on a large scale in the clinic and holds great promise for future cancer therapy.

关键词： cancer drug resistance genetic screens senescence targeted therapy

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A Roadmap toward Engineered Nitrogen-Fixing Nodule Symbiosis

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Plant Communications 2020年第1期1卷 131-146页

作者： Rik Huisman rene Geurts Wageningen University Department of Plant SciencesLaboratory of Molecular BiologyDroevendaalsesteeg 1Wageningen 6708PBThe Netherlands

In the late 19th century,it was discovered that legumes can establish a root nodule endosymbiosis with nitrogen-fixing *** after,the question was raised whether it is possible to transfer this trait to non-leguminous *** the past century,an ever-increasing amount of knowledge provided unique insights into the cellular,molecular,and genetic processes controlling this *** addition,recent phylogenomic studies uncovered several genes that evolved to function specifically to control nodule formation and bacterial ***,despite this massive body of knowledge,the long-standing objective to engineer the nitrogen-fixing nodulation trait on nonleguminous crop plants has not been achieved *** this review,the unsolved questions and engineering strategies toward nitrogen-fixing nodulation in non-legume plants are discussed and highlighted.

关键词： nodulation legumes actinorhizal plants Parasponia engineering nitrogen fixation

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Alternative approaches for estimating missing climate data:application to monthly precipitation records in South- Central Chile

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Forest Ecosystems 2018年第4期5卷 390-399页

作者： Alonso Barrios Guillermo Trincado rene Garreaud Escuela de Graduados Facultad de Ctencias Forestales y Recursos Naturales Universidad Austral de ChileBox 567ValdiviaChile Departamento de Ciencias Forestales Facuitad de Ingenieria ForestalUnlversidad del Tolima Box 6299 Ibague Colombia Instituto de Bosques y Sociedad Facultad de Ciencias Forestales y Recursos NaturalesUniversidad Austraide ChileBox 567 ValdiviaChile Departamento de Geofistca Facultad de Ciencias Fisicas y MatematicasUniversidad de ChileSantiagoChile

Background: Over the last decades interest has grown on how climate change impacts forest resources. However,one of the main constraints is that meteorological stations are riddled with missing climatic data. This study compared five approaches for estimating monthly precipitation records: inverse distance weighting(IDW), a modification of IDW that includes elevation differences between target and neighboring stations(IDW_m), correlation coefficient weighting(CCW), multiple linear regression(MLR) and artificial neural networks(ANN).Methods: A complete series of monthly precipitation records(1995-2012) from twenty meteorological stations located in central Chile were used. Two target stations were selected and their neighboring stations, located within a radius of25 km(3 stations) and 50 km(9 stations), were identified. Cross-validation was used for evaluating the accuracy of the estimation approaches. The performance and predictive capability of the approaches were evaluated using the ratio of the root mean square error to the standard deviation of measured data(RSR), the percent bias(PBIAS), and the NashSutcliffe efficiency(NSE). For testing the main and interactive effects of the radius of influence and estimation approaches,a two-level factorial design considering the target station as the blocking factor was ***: ANN and MLR showed the best statistics for all the stations and radius of influence. However, these approaches were not significantly different with IDW_m. Inclusion of elevation differences into IDW significantly improved IDW_m estimates. In terms of precision, similar estimates were obtained when applying ANN, MLR or IDW_m, and the radius of influence had a significant influence on their estimates, we conclude that estimates based on nine neighboring stations located within a radius of 50 km are needed for completing missing monthly precipitation data in regions with complex ***: It is concluded that approaches based on ANN

关键词： Climatological data Cross-validation Artificial neural networks Multiple linear regression

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Influence of the Iron Anisothermal Sintering on the Characteristic of the Hollow Cathode Discharge

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材料热处理学报 2004年第5期25卷 83-87页

作者： BRUNATTO Silvio Francisco MUZART Joel Louis rene Department of Mechanical Engineering UFPR 81531-990 Curitiba-PR BrazilDepartment of Mechanical Engineering UFSC 88040-900 Florianopolis-SC Brazil

This work studies the influence of anisothermal iron sintering process on hollow cathode discharge characteristics. Two independent cathodes form an annular discharge. The pressed cylindrical iron powder sample, acting as central cathode, was placed concentrically in the interior of an external cathode. The external cathode, machined from an AISI 310 steel bar, besides acting to confine the geometry of the plasma, can also acts as a source of alloying elements. The sample heating is a function of the ion bombardment energy and, so, of the discharge electrical parameters: current (or current density) and the effective potential applied to the cathode. Successive anisothermal sintering is performed in a same sample until the reproducibility of the electrical parameters being obtained. The heating experiments up to 1250 °C, in a gas mixture of 80% Ar + 20% H2, at pressure of 133 Pa, at flow of 2xlO"6 mV, with an inter-cathode radial space of 5.8 mm, were carried out. It was verified the metallurgical evolution of the iron sample sintering process influences the current-?0n (time switched-on of the pulse) characteristics of the discharge.

关键词：空心阴极放电等离子体焊结烧结铁溅射非等温焙烧

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Transcriptional Regulation of Nutrient Exchange in Arbuscular Mycorrhizal Symbiosis

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Molecular Plant 2018年第12期11卷 1421-1423页

作者： Erik Limpens rene Geurts Laboratory of Molecular Biology Department of Plant SciencesWageningen UniversityWageningen 6708PBThe Netherlands

Most land plants rely on the symbiosis with arbuscular mycor- rhizal (AM)fungi to acquire sufficient mineral nutrients from soils. Plants host AM fungi inside root cortex cells,where the fungus forms highly branched structures called *** are surrounded by a specialized host membrane across which nutrients are exchanged in a cooperative *** return for mineral nutrients,the fungus obtains sugars and fatty acids from the plant (Luginbuehl and Oldroyd,2017).

关键词： Transcriptional Regulation Nutrient Exchange

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