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Effects of Soluble Silicate and Nanosilica Application on Rice Nutrition in an Oxisol

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Pedosphere 2018年第4期28卷 597-606页

作者： Rita de Cassia FELIX ALVAREZ renato de mello prado Guilherme FELISBERTO Angelica Cristina FERNANdeS deUS Raimundo Leonardo LIMA de OLIVEIRA Federal University of Mato Grosso do Sul Chapadao do Sul 79560-000 (Brazil) University of the State of Sao Paulo UNESP School of Agronomic Sciences Department of Soils and Fertilizers Jaboticabal 14884-900 (Brazil)

Silicon （Si） has been supplied to plants via application of calcium silicate to soil; however, high doses of calcium silicate are required because of its low solubility. Nanoparticles can reduce Si doses and be applied to seeding furrows. This study investigated the effects of liquid Si sources, i.e., highly soluble silicate （115.2 g L^-1 Si and 60.5 g L^-1 Na20） and nanosilica （〈 200 nm）, on Si uptake by rice plants, plant lignification, plant C：N：P stoichiometry, plant physiology, and grain yield using an Oxisol under greanhouse condistions. The treatments included the application of nanosilica and soluble silicate to seeding furrows at Si doses of 0, 605, 1210, and 2 420 g ha^-1. Plant uptake and treatment effects were evaluated by measuring C and lignin contents, Si, N, and P accumulation, physiological characteristics, and grain yield of rice. The deposition of silica bodies and amorphous silica in the flag leaves was analyzed using scanning electron microscopy. Application of liquid Si increased Si accumulation in rice by 47.3% in relation to the control （0 g ha^-1 Si）, regardless of the Si sources used. Nanosilica application increased leaf lignin content by 112.7% when compared to that in the control. Silicon moderately affected the net C assimilation （increased by 1.83%） and transpiration rates （increased by 48.3%）; however, Si influenced neither plant growth nor grain yield of rice. These results are explained by the lack of biotic or abiotic stress in rice plants during the experiment. To the best of our knowledge, in Brazilian agriculture, this is the first report on the use of nanosilica as a Si fertilizer and its effect on plant nutrition. This study provides evidence that rice plants absorb and accumulate nanoparticles; however, further studies are required to investigate the use of nanoparticles in other plant species.

关键词： C:N:P stoichiometry grain yield leaf lignin net C assimilation plant physiology silicon source

The effect of abiotic stresses on plant C:N:P homeostasis and their mitigation by silicon

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Mango Tree Response to Lime Applied during the Production Phase

The Crop Journal 2024年第2期 340页

作者： Milton Garcia Costa renato de mello prado Luiz Fabiano Palaretti Jonas Pereira de Souza Júnior

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Open Journal of Soil Science 2012年第2期2卷 155-161页

作者： Eliozeas Vicente de Almeida Francisco Maximino Fernandes renato de mello prado Aparecida Conceicao Boliani Luiz de Souza Correa Sao Paulo State University Campus JaboticabalBrazil Sao Paulo State University Campus Ilha SolteiraBrazil

Tropical soils are usually highly acidic and this may hamper mango trees nutrition and production. The objective of this work was to evaluate the effects of lime doses applied to the soil surface on the plant nutritional status, the production, and the technological quality of mango fruits. The study was carried out at Selviria, in the state of Mato Grosso, Brazil, in a Typic Haplustox. Thirteen year old producing mango plants of the “Heden” variety, grafted on rootstock of the “Coquinho” variety, were used in this experiment. Lime doses of 0, 1.55, 3.10, 4.655, and 6.20 t ha–1 were applied to the soil. Each treatment was replicated 4 times and the experimental units distributed according to a randomized complete block design. Lime (CaO: 390 g kg–1;MgO: 130 g kg–1) was superficially applied to the soil and then incorporated at depths between 0 and 5 cm in the total area of the orchard. The soil chemical characteristics pH, Ca, Mg, K, sum of bases, and bases saturation, in the 0 - 20 cm layer, were evaluated 16 and 28 months after soil liming. Plant nutritional status was evaluated 12 months after soil liming. Fruit production and technological quality were evaluated during the cropping years of 2006 and 2007. Soil liming had a positive effect on the evaluated soil chemical characteristics and this improved plant nutritional status and fruit technological quality as well as increased fruit production. These beneficial effects though were observed only in the second year after soil liming. The highest fruit production was verified when soil bases saturation was of 72% and the contents of Ca and Mg were of 32 and 8 g kg–1, respectively.

关键词： Mangifera indica L. Productivity Soil Acidity Savannah

Tolerance of Urochloa brizantha ***5 to Mn Toxicity

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Open Journal of Soil Science 2013年第8期3卷 379-383页

作者： Valéria Santos Cavalcante Gustavo Caione Luiz Felipe Gevenez de Souza renato de mello prado Anderson Fernando Wamser department of Soils and fertilizers and department of Plant Production UNESP-Univ Estadual PaulistaJaboticabalSao PauloBrazil

Available information of the effects of manganese nutrition on the forage genus Urochloa is scarce. In the context, this study aims to evaluate the tolerance of Urochloa brizantha cv. MG5 to Mn toxicity. The experiment was conducted in a greenhouse at the University of the State of Sao Paulo (UNESP) in the city of Jaboticabal, SP. Plants were cultivated in vases (3.5 L) filled with soil according to a completely randomized experimental design comprising of five levels of Mn (0, 15, 30, 60 and 120 mg?dm?3) and four replications and cut in two periods: one is 42 days after sowing and the other is 30 days after the first one. Samples from both cuts were evaluated as to plant height, number of leaves and tillers, dry weight, Mn content and accumulation, and the green index was determined in the last cut. Manganese addition to soil caused an increase in chlorophyll content at the dosage of 68 mg?dm?3 observed in the second plant cut. Dosages of Mn above 15 mg?dm?3 did not induce increases in nutrient accumulation and in the number of leaves in the first and second cuts of the grass, and tillers in the first cut. The highest concentrations of manganese in the shoots did not produce visual symptoms of damage or a decrease in forage productivity demonstrating that Urochloa brizantha cv. MG5 has high tolerance to manganese toxicity.

关键词： Forage Pastures Plant Nutrition Soil Fertility Micronutrient

Use of Silicon in Mitigating Ammonium Toxicity in Maize Plants

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American Journal of Plant Sciences 2015年第11期6卷 1780-1784页

作者： Cid Naudi Silva Campos renato de mello prado Cassiano Garcia Roque Antonio Joao de Lima Neto Luiz Junior Pereira Marques Aridênia Peixoto Chaves Claudia Amaral Cruz department of Soils and Fertilizers Universidade Estadual Paulista/FCAV Jaboticabal Brazil department of Soils and Fertilizers Universidade Estadual Paulista/FCAV Jaboticabal Brazil Universidade Federal de Mato Grosso do Sul Chapad&atildeo do Sul Brazil Universidade Federal de Viçosa Vi&ccedilosa Brazil department of Applied Biology and Agriculture Universidade Estadual Paulista/FCAV Jaboticabal Brazil department of Plant Science Universidade Federal Rural do Semi-árido Mossoró Brazil M.Sc. in Agronomy Natal Brazil

Silicon is a beneficial element that can mitigate abiotic stresses, such as ammonium toxicity. The objective herein was to evaluate the effects of silicon (Si) on mitigating toxicity caused by excess ammonium in maize plants grown in nutrient solution. An experiment was conducted with maize plants (cultivar DKB 390 VT Pro II) grown in a greenhouse in pots (8 L) in a hydroponic system. The treatments were arranged in a 2 × 2 factorial, consisting of two ammonium concentrations (30 and 60 mmol·L-1) in the absence and presence of Si (10 mmol·L-1), arranged in a completely randomized design with six repetitions. At 28 days after applying the treatments the dry mass of shoots and roots was evaluated, along with accumulation of silicon and nitrogen in the shoots. The use of silicon resulted in increases in the studied variables, regardless of ammonium concentrations. Silicon reduced the effect of toxicity caused by ammonium excess in maize plants, resulting in greater growth and dry matter accumulation.

关键词： Zea mays L. Beneficial Element Nitrogen Nutritional Disorder Abiotic Stress

Selection signatures in Canchim beef cattle

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Journal of Animal Science and Biotechnology 2017年第1期8卷 15-23页

作者： Ismael Urbinati Nedenia Bonvino Stafuzza Marcos Túlio Oliveira Tatiane Cristina Seleguim Chud Roberto Hiroshi Higa Luciana Correia de Almeida Regitano Maurício mello de Alencar Marcos Eli Buzanskas Danísio prado Munari departamento de Ciencias Exatas Univ Estadual PaulistaFaculdade de Ciencias Agrairias e VeterinairiasjaboticabalSao Paulo 14884-900Brazil departamento de Tecnologia UNESP-Univ Estadual PaulistaFaculdade de Ciencias Agrarias e VeterinariasJaboticabalSao Paulo 14884-900Brazil Embrapa Agricultural Informatics CampinasSao Paulo 13083-886Brazil Embrapa Southeast Livestock Sao CarlosSao Paulo 13560-970Brazil

Background： Recent technological advances in genomics have allowed the genotyping of cattle through single nucleotide polymorphism（SNP） panels. High-density SNP panels possess greater genome coverage and are useful for the identification of conserved regions of the genome due to selection, known as selection signatures（SS）. The SS are detectable by different methods, such as the extended haplotype homozygosity（EHH）; and the integrated haplotype score（i HS）, which is derived from the EHH. The aim of this study was to identify SS regions in Canchim cattle（composite breed）, genotyped with high-density SNP ***： A total of 687,655 SNP markers and 396 samples remained for SS analysis after the genotype quality control. The i HS statistic for each marker was transformed into pi HS for better interpretation of the *** BTA5 and BTA14 showed pi HS 〉 5, with 39 and nine statistically significant SNPs（P 〈 0.00001）,respectively. For the candidate selection regions, i HS values were computed across the genome and averaged within non-overlapping windows of 500 Kb. We have identified genes that play an important role in metabolism,melanin biosynthesis（pigmentation）, and embryonic and bone ***： The observation of SS indicates that the selection processes performed in Canchim, as well as in the founder breeds（i.e. Charolais）, are maintaining specific genomic regions, particularly on BTA5 and BTA14. These selection signatures regions could be associated with Canchim characterization.

关键词： Composite breed Extended haplotype homozygosity Genomics Single nucleotide polymorphism

Water availability drives gradients of tree diversity,structure and functional traits in the Atlantic-Cerrado-Caatinga transition,Brazil

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Journal of Plant Ecology 2018年第6期11卷 803-814页

作者： Marcela de Castro Nunes Santos Terra Rubens Manoel dos Santos Jamir Afonso do prado Júnior José Marcio de mello José Roberto Soares Scolforo Marco Aurélio Leite Fontes Ivan Schiavini Aliny Aparecida dos Reis Inacio Thomaz Bueno Luiz Fernando Silva Magnago Hans ter Steege departamento de Ciências Florestais Universidade Federal de LavrasCampus UniversitárioPO Box 3037LavrasMinas Gerais 37200-000Brazil Instituto de Biologia Universidade Federal de UberlândiaCampus Umuarama-Bloco 2DPO Box 593UberlândiaMinas Gerais 38400-902Brazil Applied Ecology and Conservation Lab Programa de Pós‐graduação Ecologia e Conservação da BiodiversidadeUniversidade Estadual de Santa CruzIlhéusBahia 45662-900Brazil Naturalis Biodiversity Center Vondellaan 55Postbus 95172300 RA LeidenThe Netherlands Coordenação de Botânica Museu Paraense Emílio GoeldiAv.Magalhães Barata 376PO Box 399BelémPara´66040-170Brazil

Aims Climate and soil are among the most important factors determin-ing variation in tree communities,but their effects have not been thoroughly elucidated to date for many vegetation *** this study,we evaluate how climate and soil gradients affect gradients of vegetation composition,species diversity and dominance,structure and functional traits(seed mass and wood density)using over 327000 trees in 158 sites distributed along environmental gradients in the transitions among the Atlantic forest,Cerrado and Caatinga in Minas Gerais State(MG),Brazil(nearly 600000 km2).Methods Gradients in species,genus and family abundance in addition to basal area,stem density,species diversity(Fisher’s alpha),domin-ance percentage,seed mass and wood density were correlated using multiple regressions with environmental variables,as summarized in four principal component analysis axes(two climatic-precipi-tation seasonality and temperature range-and two edaphic-soil fertility and soil moisture).Additionally,ordinary kriging maps were used to better illustrate the *** Findings Multiple regression models indicate that all variables but domin-ance percentage were affected by one or more of the environmental gradients,but the average R2 was low(26.25%).Kriging maps rein-forced the patterns observed in the regression *** seasonality and soil moisture gradients were the most important gra-dients affecting vegetation *** finding suggests that water availability is an important determinant of vegetation features in these vegetation transitions.

关键词： climate environmental gradient soil southeastern Brazil vegetation

Candidate genes for male and female reproductive traits in Canchim beef cattle

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Journal of Animal Science and Biotechnology 2018年第1期9卷 59-68页

作者： Marcos Eli Buzanskas Daniela do Amaral Grossi Ricardo Vieira Ventura Flavio Schramm Schenkel Tatiane Cristina Seleguim Chud Nedenia Bonvino Stafuzza Luciana Diniz Rola Sarah Laguna Conceio Meirelles Fabiana Barichello Mokry Maurício de Alvarenga Mudadu Roberto Hiroshi Higa Marcos Vinícius Gualberto Barbosa da Silva Maurício mello de Alencar Luciana Correia de Almeida Regitano Danísio prado Munari departamento de Zootecnia Universidade Federal da Paraíba(UFPB)AreiaParailba 58397-000Brazil Fast Genetics SaskatoonSK S7K 2K6Canada Beef Improvement Opportunities(BIO) GuelphON N1K 1ESCanada department of Animal and Poultry Science University of GuelphCentre for Genetic Improvement of Livestock(CGIL)GuelphON N1G 2W1Canada departamento de Ciencias Exatas Faculdade de Ci6ncias Agrarias e VeterinariasUniversidade Estadual Paulista(Unesp)JaboticabalSao Paulo 14884 900Brazil departamento de Zootecnia Nucleo de Pesquisa e Conservacao de CervMeosFaculdade de Ciencias Agrarias e VeterinariasUniversidade Estadual Paulista(Unesp)JaboticabalSao Paulo 14884 900Brazil department of Animal Science Federal University/of Lavras(UFLA)LavrasMinas Gerais 37200 000Brazil department of Genetics and Evolution Federa University of Sao Carlos(UFSCar)Sao CarlosSao Paulo 13565-905Brazil Embrapa Agricultural Informatics Campinas55o Paulo 13083 886Brazil Embrapa Dairy Cattle Juiz de ForaMinas Gerais 36038 330Brazil Embrapa Southeast Livestock Sao CarlosSao Paulo 13560 970Brazil

Background： Beef cattle breeding programs in Brazil have placed greater emphasis on the genomic study of reproductive traits of males and females due to their economic importance. In this study, genome-wide associations were assessed for scrotal circumference at 210 d of age, scrotal circumference at 420 d of age, age at first calving, and age at second calving, in Canchim beef cattle. Data quality control was conducted resulting in 672,778 SNPs and 392 ***： Associated SNPs were observed for scrotal circumference at 420 d of age（435 SNPs）, followed by scrotal circumference at 210 d of age（12 SNPs）, age at first calving（six SNPs）, and age at second calving（four SNPs）. We investigated whether significant SNPs were within genic or surrounding regions. Biological processes of genes were associated with immune system, multicellular organismal process, response to stimulus, apoptotic process, cellular component organization or biogenesis, biological adhesion, and ***： Few associations were observed for scrotal circumference at 210 d of age, age at first calving, and age at second calving, reinforcing their polygenic inheritance and the complexity of understanding the genetic architecture of reproductive traits. Finding many associations for scrotal circumference at 420 d of age in various regions of the Canchim genome also reveals the difficulty of targeting specific candidate genes that could act on fertility; nonetheless,the high linkage disequilibrium between loci herein estimated could aid to overcome this issue. Therefore, al relevant information about genomic regions influencing reproductive traits may contribute to target candidate genes for further investigation of causal mutations and aid in future genomic studies in Canchim cattle to improve the breeding program.

关键词： Animal breeding Composite breed Genome-wide association Genomic data Single nucleotide polymorphism

Scar Acceleration Method—MAC^® in the Treatment of Chronic Wounds in Lymphedema: Case Report

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Open Journal of Regenerative Medicine 2022年第4期11卷 63-76页

作者： Marcus Vinicius de mello Pinto Aline Ronis Sampaio Miriam Viviane Baron Angela Gonçalves Marx Esteban Fortuny Francisco Javier Millan Martínez Alessandra Fernandes de Lima Thais do prado Pessanha Passos Alexandre Gomes Sancho Celulare Institute Petrópolis Brazil Program in Medicine and Health Sciences of the Pontifical Catholic University of Rio Grande do Sul (PUC/RS) Porto Alegre Brazil Faculty of Medicine of USP Sao Paulo Brazil Universidad Finis Terrae Santiago Chile Hospital Viamed Virgen de la Paloma Madrid Spain Clinilar Belo Horizonte Brazil Oncology Physiotherapy by ABFO COFFITO Brazil Physiotherapy Course Grande Rio University Duque de Caxias Brazil Augusto Motta University Center (UNISUAM) Rio de Janeiro Brazil

Wounds are a serious public health problem in our country. Access to effective, low-cost treatment is still far from the reality for many patients leading to a chronic and serious condition. They interfere in the quality of life of these individuals who isolate themselves socially constrained by the appearance of their wounds. The report shows the case of a patient with type II diabetes mellitus, with chronic wounds in lymphedema in the lower limbs who was treated with the Scar Acceleration Methodology associated with Complex decongestive Therapy, leading to an improvement in her social participation and quality of life.

关键词： Lympedema Wound Healing Diabetes Mellitus Complication