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Application of exosome-derived noncoding RNAs in bone regeneration:Opportunities and challenges

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World Journal of Stem Cells 2022年第7期14卷 473-489页

作者： yuan-Zhong ren Shan-Shan Ding Ya-Ping jiang Hui Wen Tao Li Department of Emergency Trauma Surgery Luoyang Central Hospital Affiliated to Zhengzhou UniversityLuoyang 471000Henan ProvinceChina Department of Geriatrics Luoyang Central Hospital Affiliated to Zhengzhou UniversityLuoyang 471000Henan ProvinceChina Department of Oral Implantology The Affiliated Hospital of Qingdao UniversityQingdao 266000Shandong ProvinceChina Department of Joint Surgery The Affiliated Hospital of Qingdao UniversityQingdao 266003Shandong ProvinceChina

With advances in the fields of regenerative medicine,cell-free therapy has received increased *** have a variety of endogenous properties that provide stability for molecular transport across biological barriers to cells,as a form of cell-to-cell communication that regulates function and *** addition,exosomes are an important component of paracrine signaling in stemcell-based therapy and can be used as a stand-alone therapy or as a drug delivery *** remarkable potential of exosomes has paved the pathway for cell-free treatment in bone *** are enriched in distinct noncoding RNAs(ncRNAs),including microRNAs,long ncRNAs and circular *** ncRNAs have multiple *** expression of ncRNA in exosomes is associated with the regenerative potential and development of various diseases,such as femoral head osteonecrosis,myocardial infarction,and *** there is increasing evidence that exosome-derived ncRNAs(exoncRNAs)have the potential for bone regeneration,the detailed mechanisms are not fully ***,we review the biogenesis of exo-ncRNA and the effects of ncRNAs on angiogenesis and osteoblast-and osteoclast-related pathways in different ***,there are still many unsolved problems and challenges in the clinical application of ncRNA;for instance,production,storage,targeted delivery and therapeutic potency *** in exo-ncRNA methods and design will promote the development of therapeutics,revolutionizing the present landscape.

关键词： Exosomes Non-coding RNA Bone Osteogenesis Angiogenesis Osteoclasts

Deciphering fungal metabolon coupling tandem inverse-electrondemand Diels-Alder reaction and semipinacol rearrangement for the biosynthesis of spiro polycyclic alkaloids

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Science China Chemistry 2025年第01期 288-296页

作者： Shuai Liu Wen-Qiang Xu Ying-Tong Di Man-Cheng Tang Ding-Kang Chen Ming-Ming Cao Yao-Wen Chang Hong-Yu Tang Chun-Mao yuan Jun-Bo Yang Zhi-Li Zuo Han Guo Zi-Fei Xu Ying Zeng Yun-Dong Wu Xiao-jiang Hao Key Laboratory of Phytochemistry and Natural Medicines (Chinese Academy of Sciences) Kunming Institute of Botany Chinese Academy of Sciences University of Chinese Academy of Sciences Lab of Computational Chemistry and Drug Design State Key Laboratory of Chemical Oncogenomics Peking University Shenzhen Graduate School Shenzhen Bay Laboratory State Key Laboratory of Microbial Metabolism School of Life Sciences & Biotechnology Shanghai Jiao Tong University State Key Laboratory of Functions and Applications of Medicinal Plants Guizhou Medical University Molecular Biology Experimental Center Germplasm Bank of Wild Species in Southwest China Kunming Institute of Botany Chinese Academy of Sciences Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences Research Unit of Chemical Biology of Natural Anti-Virus Products Chinese Academy of Medical Sciences

In the intricate process of natural product biosynthesis, a metabolon can enhance metabolic flux by associating sequential enzymes. A fungal metabolon, comprising of flavin-dependent monooxygenase Spe F and P450 monooxygenase Spe G, is identified in the biosynthesis of spiro polycyclic alkaloids(+)-notoamide B and its diastereomer(+)-versicolamide B. Using notoamide E as a substance, Spe F/Spe G metabolon can control the stereoselectivity of its 2,3-epoxidation, followed by hydrogen atom ion at C-17 to generate reactive epoxide tau-m A with dienyl iminium unit. Subsequently,(+)-notoamide B and(+)-versicolamide B are produced via tandem nonenzymatic inverse-electron-demand Diels-Alder reaction and semipinacol rearrangement. This provides the first example of metabolon in the biosynthesis of spiro-prenylated indole alkaloids.

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Analysis on electrical transport characteristic of 8-hydroquinoline aluminum film

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Chinese Science Bulletin 1998年第2期43卷 121-124页

作者： Ligong Zhang Dapeng jiang Xinguang ren Xueyan Liu Yajun Li Ande Lu Jinshan yuan Chinese Acad Sci Changchun Inst Phys Changchun 130021 Peoples R China

The current_voltage and capacitance_voltage characteristic of the organic single_layered electroluminescent diode utilizing 8_hydroquinoline aluminum as active layer have been measured under bias ranging from -5 V to 28 V in this work. A simple model for charge transport process of 8_hydroquinoline aluminum layer is proposed to illuminate the conductivity characteristic of the diode.

关键词： hydroquinoline aluminum current-voltage characteristic capacitance-voltage characteristic.

Cell-free synthetic biology in the new era of enzyme engineering

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Chinese Journal of Chemical Engineering 2020年第11期28卷 2810-2816页

作者： Nan jiang Lianju Ma yuan Lu College of Life Sciences Shenyang Normal UniversityShenyang 110034China Key Lab of Industrial Biocatalysis Ministry of EducationDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China

With the gradual rise of enzyme engineering,it has played an essential role in synthetic biology,medicine,and ***,due to the limitation of the cell membrane,the complexity of cellular metabolism,the difficulty of controlling the reaction environment,and the toxicity of some metabolic products in traditional in vivo enzyme engineering,it is usually problematic to express functional enzymes and produce a high yield of synthesized ***,cell-free synthetic biology methods for enzyme engineering have been proposed as alternative *** cell-free method has no limitation of the cell membrane and no need to maintain cell viability,and each biosynthetic pathway is highly *** property makes cell-free approaches suitable for the production of valuable products such as functional enzymes and chemicals that are difficult to *** article aims to discuss the latest advances in cell-free enzyme engineering,assess the trend of this developing topical filed,and analyze its prospects.

关键词： Cell-free synthetic biology Enzyme engineering Metabolic engineering

Conductive,Stretchable,and Self-healing Ionic Gel Based on Dynamic Covalent Bonds and Electrostatic Interaction

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Chinese Journal of Polymer Science 2019年第11期37卷 1053-1059页

作者： Yi Sun Yong-yuan ren Qi Li Rong-Wei Shi Yin Hu jiang-Na Guo Zhe Sun Feng Yan Department of Polymer Science and Engineering College of Chemistry Chemical Engineering and Materials Science Soochow University

Integrating multiple functions into one gel that can be widely applied to electronic devices as well as chemical and biomedical engineering remains a big ***,a multifunctional ionic liquid/dynamic covalent bonds (ionic/DCB) type gel was designed and synthesized via one-pot *** the assistance of electrostatic interaction provided by the imidazolium cations of IL and the reversible DCB of boronic ester,as-prepared ionic/DCB gel showed good stretchable properties and high ionic conductivity at ambient *** addition,the electrostatic interaction between imidazolium cations and sulfonate anions and the reversible DCB led to enhanced chain mobility and thereby excellent self-healing ***,sulfonate anions in ionic/DCB gel could alleviate the migration of electronegative polysulfide and promote the transportation of electropositive lithium ion in lithium-sulfur battery ***,this work provides a new insight to promote the current research on self-healing gels,hopefully expanding their applications in electronic devices.

关键词： Ionic gel Conductivity Self-healing Dynamic covalent bonds Lithium-sulfur battery

Nanoelectrochemical architectures for high-spatial-resolution single cell analysis

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Science China Chemistry 2017年第10期60卷 1277-1284页

作者： Junyu Zhou Dechen jiang Hong-yuan Chen State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Nanjing 210093 China

The analysis of single cells instead of cell populations is important for characterizing cellular heterogeneity and elucidating the cellular signalling pathways. Nanoelectrodes have emerged as an increasingly important tool for biomolecule analyses at the single-cell level with high spatial or temporal resolution. Various electrochemical methods, such as amperometry and scanning electrochemical microscopy(SECM), have been applied. Research to date has focused on the development of new nanoelectrochemical architectures, such as arrays, to achieve higher spatial resolution and faster analysis rates for single-cell analysis. In this review, the fabrication of these new nanoelectrochemical architectures and their applications in high spatial resolution single-cell analyses are discussed. The recent progress of Chinese researchers is highlighted.

关键词： nanoelectrochemical architectures single-cell analysis high spatial resolution high throughput

A Test Pattern Identification Algorithm and Its Application to CINRAD/SA(B) Data

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Advances in Atmospheric Sciences 2014年第2期31卷 331-343页

作者： jiang yuan LIU Liping State Key Laboratory of Severe Weather Chinese Academy of Meteorological Sciences Nanjing University of Information Science and Technology National Meteorological Center

A variety of faulty radar echoes may cause serious problems with radar data applications,especially radar data assimilation and quantitative precipitation *** this study,＂test pattern＂ caused by test signal or radar hardware failures in CINRAD （China New Generation Weather Radar） SA and SB radar operational observations are *** order to distinguish the test pattern from other types of radar echoes,such as precipitation,clear air and other non-meteorological echoes,five feature parameters including the effective reflectivity data percentage （Rz）,velocity RF （range folding） data percentage （RRF）,missing velocity data percentage （RM）,averaged along-azimuth reflectivity fluctuation （RNr,z） and averaged along-beam reflectivity fluctuation （RNa,z） are *** on the fuzzy logic method,a test pattern identification algorithm is developed,and the statistical results from all the different kinds of radar echoes indicate the performance of the *** of two typical cases with heavy precipitation echoes located inside the test pattern are *** statistical results show that the test pattern identification algorithm performs well,since the test pattern is recognized in most ***,the algorithm can effectively remove the test pattern signal and retain strong precipitation echoes in heavy rainfall events.

关键词： quality control test pattern fuzzy logic radar data

Boosting multi-features with prior knowledge for mini unmanned helicopter landmark detection

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2008年第2期9卷 256-261页

作者： Qin-yuan ren Ping LI Bo HAN The State Key Laboratory of Industrial Control Technology Zhejiang University Hangzhou 310027 China

Without sufficient real training data,the data driven classification algorithms based on boosting method cannot solely be utilized to applications such as the mini unmanned helicopter landmark image *** this paper,we propose an approach which uses a boosting algorithm with the prior knowledge for the mini unmanned helicopter landmark image *** stage forward stagewise additive model of boosting is analyzed,and the approach how to combine it with the prior knowledge model is *** approach is then applied to landmark image detection,where the multi-features are boosted to solve a series of problems,such as rotation,noises affected,*** of real flight experiments demonstrate that for small training examples the boosted learning system using prior knowledge is dramatically better than the one driven by data only.

关键词： Boosting Prior knowledge model Landmark detection

Endostatin inhibits hypertrophic scarring in a rabbit ear model

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第3期14卷 224-230页

作者： Hai-tao ren Hang HU yuan LI Hong-fei jiang Xin-lei HU Chun-mao HAN Department of Burns and Wound Center the Second Affiliated HospitalSchool of MedicineZhejiang University Department of Ultrasound Women's HospitalSchool of MedicineZhejiang University Assisted Reproduction Unit Department of Obstetrics and GynecologySir Run Run Shaw HospitalSchool of MedicineZhejiang University

Objective： The present study was designed to use an in vivo rabbit ear scar model to investigate the efficacy of systemic administration of endostatin in inhibiting scar formation. Methods： Eight male New Zealand white rabbits were randomly assigned to two groups. Scar model was established by making six full skin defect wounds in each ear. For the intervention group, intraperitoneal injection of endostatin was performed each day after the wound healed （about 15 d post wounding）. For the control group, equal volume of saline was injected. Thickness of scars in each group was measured by sliding caliper and the scar microcirculatory perfusion was assessed by laser Doppler flowmetry on Days 15, 21, 28, and 35 post wounding. Rabbits were euthanatized and their scars were harvested for histological and proteomic analyses on Day 35 post wounding. Results： Macroscopically, scars of the control group were thicker than those of the intervention group. Significant differences between the two groups were observed on Days 21 and 35 （p〈0.05）. Scar thickness, measured by scar elevation index （SEI） at Day 35 post wounding, was significantly reduced in the intervention group （1.09±0.19） compared with the controls （1.36±0.28）. Microvessel density （MVD） observed in the intervention group （1.73±0.94） was significantly lower than that of the control group （5.63±1.78） on Day 35. The distribution of collagen fibers in scars treated with endostatin was relatively regular, while collagen fibers in untreated controls were thicker and showed disordered alignment. Western blot analysis showed that the expressions of type I collagen and Bcl-2 were depressed by injection of endostatin. Conclusions： Our results from the rabbit ear hypertrophic scar model indicate that systemic application of endostatin could inhibit local hypertrophic scar formation, possibly through reducing scar vascularization and angiogenesis. Our results indicated that endostatin may promote the apoptosis of endothelial ce

关键词： Endostatin Hypertrophic scar Systemic administration