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Polymerization of fluorescent analogue of plant actin in vitro and in vivo

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Chinese Science Bulletin 2000年第17期45卷 1583-1586页

作者： ren Haiyun yuan Ming Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education College of Life SciencesBeijing Normal UniversityBeijing 100875China Key Laboratory of Plant Physiology and Biochemistry of Ministry of Agriculture Department of Plant SciencesCollege of Biological SciencesChina Agricultural UniversityBeijing 100094China

Maize pollen actin has been labeled with Oregon Green 488 iodoacetamide. A yield of 3 mg fluorescent actin analogue has been obtained from 10 mg of maize pollen actin, which is 99% in purity and the dye/protein ratio is 72%. In the presence of Mg2+ and K+, the fluorescent actin analogue polymerized into filaments in vitro. Green fluorescent filaments were observed when the fluorescent actin was introduced into living plant cells by microinjection, indicating that the fluorescent actin analogue functions similarly to the native actin.

关键词： fluorescent actin analogue actin filaments microinjection.

What control the spatial patterns and predictions of runoff response over the contiguous USA?

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Journal of Geographical Sciences 2024年第7期34卷 1297-1322页

作者： jiang Shanhu DU Shuping ren Liliang GONG Xinglong YAN Denghua yuan Shanshui LIU Yi YANG Xiaoli XU Chongyu The National Key Laboratory of Water Disaster Prevention Hohai UniversityNanjing210098China College of Hydrology and Water Resources Hohai UniversityNanjing210098China School of Water Conservancy&Civil Engineering Northeast Agricultural UniversityHarbin150030China Department of Water Resources China Institute of Water Resources and Hydropower ResearchBeijing100038China Department of Geosciences University of OsloOsloNorway

Understanding the nonlinear relationship between hydrological response and key factors and the cause behind this relationship is vital for water resource management and earth system *** this study,we undertook several steps to explore the ***,we partitioned runoff response change(RRC)into multiple components associated with climate and catchment properties,and examined the spatial patterns and smoothness indicated by the Moran's Index of RRC across the contiguous United States(CONUS).Subsequently,we employed a machine learning model to predict RRC using catchment attribute predictors encompassing climate,topography,hydrology,soil,land use/cover,and ***,we identified the primary factors influencing RRC and quantified how these key factors control RRC by employing the accumulated local effect,which allows for the representation of not only dominant but also secondary ***,we explored the relationship between ecoregion patterns,climate gradients,and the distribution of RRC across *** findings indicate that:(1)RRC demonstrating significant connections between catchments tends to be well predicted by catchment attributes in space;(2)climate,hydrology,and topography emerge as the top three key attributes nonlinearly influencing the RRC patterns,with their second-order effects determining the heterogeneous patterns of RRC;and(3)local Moran's I signifies a collaborative relationship between the patterns of RRC and their spatial smoothness,climate space,and ecoregions.

关键词： hydrological response prediction machine learning accumulated local effect Moran’s Index large-sample study

An Adaptive Regulation Problem and Its Application

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International Journal of Automation and computing 2017年第2期14卷 221-228页

作者： yuan jiang Ji-Yang Dai Key Laboratory of Image Processing and Pattern Recognition Nanchang Hangkong University Nanchang 330063 China School of Information Engineering Nanchang Hangkong University Nanchang 330063 China

This paper studies an adaptive regulation problem for the modified FitzHugh-Nagumo neuron model under external electrical stimulation. We first present the solution of the global robust output regulation problem for output feedback system with an uncertain exosystem which models the external electrical stimulation with unknown frequency and amplitude. Then, we show that the robust control problem for the modified FitzHugh-Nagumo neuron model can be formulated as the global robust output regulation problem and the solvability conditions for the output regulation problem for the modified FitzHugh-Nagumo neuron model are all satisfied. Then, we apply the obtained output regulation result to constructing an output feedback control law for the modified FitzHugh-Nagumo neuron model to achieve global stability of the closed-loop system in the presence of uncertain parameters and external stimulus. An example is given to show that the proposed algorithm can completely reject the external electrical stimulation.

关键词： Control theory Lyapunov methods internal model principle modified FitzHugh-Nagumo model.

Targeting TGFβ signal transduction for cancer therapy

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Signal Transduction and Targeted Therapy 2021年第2期6卷 277-296页

作者： Sijia Liu jiang ren Peter ten Dijke Oncode Institute and Department of Cell and Chemical Biology Leiden University Medical CenterEinthovenweg 202300 RC LeidenThe Netherlands

Transforming growth factor-β(TGFβ) family members are structurally and functionally related cytokines that have diverse effects on the regulation of cell fate during embryonic development and in the maintenance of adult tissue *** of TGFβ family signaling can lead to a plethora of developmental disorders and diseases,including cancer,immune dysfunction,and *** this review,we focus on TGFβ,a well-characterized family member that has a dichotomous role in cancer progression,acting in early stages as a tumor suppressor and in late stages as a tumor *** functions of TGFβ are not limited to the regulation of proliferation,differentiation,apoptosis,epithelial-mesenchymal transition,and metastasis of cancer *** reports have related TGFβ to effects on cells that are present in the tumor microenvironment through the stimulation of extracellular matrix deposition,promotion of angiogenesis,and suppression of the ant-tumor immune *** pro-oncogenic roles of TGFβ have attracted considerable attention because their intervention provides a therapeutic approach for cancer ***,the critical function of TGFβin maintaining tissue homeostasis makes targeting TGFβa ***,we review the pleiotropic functions of TGFβin cancer initiation and progression,summarize the recent clinical advancements regarding TGFβ signaling interventions for cancer treatment,and discuss the remaining challenges and opportunities related to targeting this *** provide a perspective on synergistic therapies that combine anti-TGFβ therapy with cytotoxic chemotherapy,targeted therapy,radiotherapy,or immunotherapy.

关键词： TGFβ homeostasis stimulation

Draft genome of an Aerophobetes bacterium reveals a facultative lifestyle in deep-sea anaerobic sediments

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Science Bulletin 2016年第15期61卷 1176-1186页

作者： Yong Wang Zhao-Ming Gao jiang-Tao Li Salim Bougouffa ren Mao Tian Vladimir B. Bajic Pei-yuan Qian Sanya Institute of Deep Sea Science and Engineering ChineseAcademy of Sciences Sanya 572000 China Division of Life Science Hong Kong University of Science and Technology Clear Water Bay Hong Kong China State Key Laboratory of Marine Geology Tongji UniversityShanghai 200092 China Computational Bioscience Research Center King Abdullah University of Science and Technology Thuwal Jeddah 23955-6900 Saudi Arabia

Aerophobetes （or CD12） is a recently defined bacterial phylum, of which the metabolic processes and ecological importance remain unclear. In the present study, we obtained the draft genome of an Aerophobetes bac- terium TCSI from saline sediment near the Thuwal cold seep in the Red Sea using a genome binning method. Analysis of 16S rRNA genes of TCS1 and close relatives revealed wide distribution of Aerophobetes in deep-sea sediments. Phylogenetic relationships showed affinity between Aerophobetes TCS 1 and some thermophilic bac- terial phyla. The genome of TCS1 （at least 1.27 Mbp） contains a full set of genes encoding core metabolic path- ways, including glycolysis and pyruvate fermentation to produce acetyl-CoA and acetate. The identification of cross-membrane sugar transporter genes further indicates its potential ability to consume carbohydrates preserved inthe sediment under the microbial mat. Aerophobetes bac- terium TCS1 therefore probably carried out saccharolytic and fermentative metabolism. The genes responsible for autotrophic synthesis of acetyl-CoA via the Wood-Ljung- dahl pathway were also found in the genome. Phylogenetic study of the essential genes for the Wood-Ljungdahl pathway implied relative independence of Aerophobetes bacterium from the known acetogens and methanogens. Compared with genomes of acetogenic bacteria, Aero- phobetes bacterium TCS 1 genome lacks the genes involved in nitrogen metabolism, sulfur metabolism, signal trans- duction and cell motility. The metabolic activities of TCS 1 might depend on geochemical conditions such as supplies of CO2, hydrogen and sugars, and therefore the TCSI might be a facultative bacterium in anaerobic saline sedi- ments near cold seeps.

关键词： Deep-sea sediment Aerophobetes Metagenome Sugar fermentation Cold seep

Facile synthesis of molybdenum carbide nanoparticles in situ decorated on nitrogen-doped porous carbons for hydrogen evolution reaction

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Journal of Energy Chemistry 2019年第5期28卷 78-84页

作者： Jin-Tao ren Yue-Jun Song Zhong-Yong yuan National Institute for Advanced Materials School of Materials Science and EngineeringNankai UniversityTianjin 300350China Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education) Collaborative Innovation Center of Chemical Science and Engineering (Tianjin)Nankai UniversityTianjin 300071China

Molybdenum-based electrocatalysts are promising candidates of platinum (Pt)-based materials in electrocatalyzing hydrogen evolution reaction (HER), due to their cost-efficient and resembled electronic properties. Reported herein is the preparation of molybdenum carbide nanoparticles uniformly decorated on nitrogen-modified carbons (Mo2C/NC) through the carbonization of Mo-based polymers under hydrogen atmosphere by using poly(p-phenylenediamine) and ammonium heptamolybdate polymer analogue as precursors. And the molybdenum nitride nanoparticles loaded on porous N-doped carbons (Mo2N/NC) are also fabricated by calcination the polymer precursors in nitrogen gas. The Mo2C/NC shows more excellent electrocatalytic activity than Mo2N/NC in 0.5 M H2SO4, together with robust long-term durability. The well-crystalline nanoparticles and the increased electron conductivity are the main characters responded for the high catalytic efficiency of the fabricated electrocatalysts. This easily fabrication procedure may provide a facile route to prepare non-noble metal carbide/nitride catalysts featuring wellengineered structural and textural peculiarities for realistic energy conversion system.

关键词： Molybdenum carbide Molybdenum nitride Nitrogen-doped carbon Hydrogen evolution reaction Electrocatalysis

Developer recommendation on bug commenting:a ranking approach for the developer crowd

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Science China(Information Sciences) 2017年第7期60卷 159-176页

作者： Jifeng XUAN He jiang Hongyu ZHANG Zhilei ren State Key Laboratory of Software Engineering School of Computer Wuhan University School of Software Dalian University of Technology Microsoft Research Asia School of Electrical Engineering and Computer Science The University of Newcastle

A bug tracking system provides a collaborative platform for the developer crowd. After a bug report is submitted, developers can make comments to supplement the details of the bug report. Due to the large number of developers and bug reports, it is hard to determine which developer(also called commenter) is able to comment on a particular bug report. We refer to the problem of recommending developers for commenting on bug reports as commenter recommendation. In this paper, we perform an empirical analysis on commenter recommendation based on five-year bug reports of four open source projects. First, we preliminarily analyze bug comments and commenters in three categories, the relationship between commenters and fixers, the data scale of comments, and the collaboration on bug commenting. Second, we design a recommendation approach via ranking developers in the crowd to reduce the manual effort of identifying commenters. In this approach,we formulize the commenter recommendation problem as a multi-label recommendation task and leverage both developer collaboration and bug content to find out appropriate commenters. Experimental results show that our approach can effectively recommend commenters; 41% to 75% of the recall value is achieved for top-10 recommendation. Our empirical analysis on bug commenting can help developers understand and improve the process of fixing bugs.

关键词： developer recommendation bug comments empirical analysis recommendation for the crowd collaborative filtering software repositories

Asymptotic stability of solutions to the nonisentropic hydrodynamic model for semiconductors

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Applied Mathematics(A Journal of Chinese Universities) 2008年第2期23卷 151-167页

作者： XU jiang FANG Dao-yuan Dept. of Math. Nanjing Univ. of Aeronautics and Astronautics Nanjing 210016 China Dept. of Math. Zhejiang Univ. Hangzhou 310027 China

In this paper, the asymptotic stability of smooth solutions to the multidimensional nonisentropic hydrodynamic model for semiconductors is established, under the assumption that the initial data are a small perturbation of the stationary solutions for the thermal equilibrium state, whose proofs mainly depend on the basic energy methods.

关键词： asymptotic stability smooth solutions hydrodynamic model semiconductor.

Continuous Separation of Multiple Size Microparticles using Alternating Current Dielectrophoresis in Microfluidic Device with Acupuncture Needle Electrodes

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Chinese Journal of Mechanical Engineering 2016年第2期29卷 325-331页

作者： TAO Ye ren Yukun YAN Hui jiang Hongyuan School of Mechatronics Engineering Harbin Institute of Technology State Key Laboratory of Fluid Power Transmission and Control Zhejiang University

The need to continuously separate multiple microparticles is required for the recent development of lab-on-chip technology. Dielectrophoresis（DEP）-based separation device is extensively used in kinds of microfluidic applications. However, such conventional DEP-based device is relatively complicated and difficult for fabrication. A concise microfluidic device is presented for effective continuous separation of multiple size particle mixtures. A pair of acupuncture needle electrodes are creatively employed and embedded in a PDMS（poly-dimethylsiloxane） hurdle for generating non-uniform electric field thereby achieving a continuous DEP separation. The separation mechanism is that the incoming particle samples with different sizes experience different negative DEP（n DEP） forces and then they can be transported into different downstream outlets. The DEP characterizations of particles are calculated, and their trajectories are numerically predicted by considering the combined action of the incoming laminar flow and the n DEP force field for guiding the separation experiments. The device performance is verified by successfully separating a three-sized particle mixture, including polystyrene microspheres with diameters of 3 μm, 10 μm and 25 μm. The separation purity is below 70% when the flow rate ratio is less than 3.5 or more than 5.1, while the separation purity can be up to more than 90% when the flow rate ratio is between 3.5 and 5.1 and meanwhile ensure the voltage output falls in between 120 V and 150 V. Such simple DEP-based separation device has extensive applications in future microfluidic systems.

关键词： continuous separation of multiple size particles dielectrophoresis acupuncture needle electrodes microfluidic