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Genotoxicity evaluation and a primary risk assessment of organic pollutants in the drinking water sources of Nanjing, China

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Journal of Environmental Sciences 2006年第5期18卷 983-988页

作者： LI yi-qiang WU Yu-lin CHEN Yuan-gao kong Zhi-ming The State Key Laboratory of Pollution Control and Resource Reuse School of Environmental Nanjing University Nanjing 210093 China National Accredited Laboratory Environmental Monitoring Center of Jiangsu Province Nanjing 210036 China

An increasing number of industrial, agricultural, and commercial chemicals in the aquatic environment leads to various deleterious effects on organisms, which is becoming an increasingly serious problem in China. In this study, the comet assay was conducted to investigate the genotoxicity to human body caused by organic concentrates in the drinking water sources of Nanjing City from Yangtze River of China, and health and ecology risk due to expose to these organic pollutants were evaluated with the multimedia environmental assessment system （MEAS）. For all the water samples, they were collected from four different locations in the drinking water sourcr samples, es of Nanjing City. The results of the comet assay showed that all the organic concentrates from the water samples could induce different levels DNA damages on human peripheral blood lymphocytes, and a statistically significant difference （p〈0.01） was observed compared with the solvent control, which demonstrated the genotoxicity was in existence. According to the ambient severity （AS） of individual compound, we had sorted out the main organic pollutants in the drinking water source of the four waterworks, and the results showed that there was some potential hazard to human body for all the source water, namely the total ambient severity （TAS） of health for each water source was more than 1. However, the TAS of ecology for each water source was less than 1, which indicated that it was safe to ecology. The results of this investigation demonstrate the application of the comet assay and the MEAS in aquatic environmental monitoring studies, and the comet assay found to be fast, sensitive, and suitable for genotoxicity monitoring programs of drinking water source.

关键词： genotoxicity comet assay risk assessment organic pollutants drinking water sources Nanjing City

Interaction between Rice stripe virus Disease- Specific Protein and Host PsbP Enhances Virus Symptoms

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Molecular Plant 2014年第4期7卷 691-708页

作者： Lingfang kong Jianxiang Wu Lina Lu yi Xu Xueping Zhou State Key Laboratory of Rice Biology Institute of Biotechnology Zhejiang University Hangzhou 310058 People's Republic of China

Rice stripe virus （RSV） causes severe diseases in Oryza sativa （rice） in many Eastern Asian countries. Diseasespecific protein （SP） of RSV is a non-structural protein and its accumulation level in rice plant was shown to determine the severity of RSV symptoms. Here, we present evidence that expression of RSV SP alone in rice or Nicotiana benthamiana did not produce visible symptoms. Expression of SP in these two plants, however, enhanced RSV- or Potato virus X （PVX）- induced symptoms. Through yeast two-hybrid screening, GST pull-down, and bimolecular fluorescence complementation assays, we demonstrated that RSV SP interacted with PsbP, a 23-kDa oxygen-evolving complex protein, in both rice and N. benthamiana. Furthermore, our investigation showed that silencing of PsbP expression in both plants increased disease symptom severity and virus accumulation. Confocal microscopy using N, benthamiana protoplast showed that PsbP accu- mulated predominantly in chloroplast in wild-type N. benthamiana cells. In the presence of RSV SP, most PsbP was recruited into cytoplasm of the assayed cells. In addition, accumulation of SP during RSV infection resulted in alterations of chloroplast structure and function. Our findings shed light on the molecular mechanism underlying RSV disease symptom development.

关键词： Rice stripe virus disease-specific protein interaction PsbP symptom.

Phylogenomic resolution of Imparidentia(Mollusca:Bivalvia)diversifcation through mitochondrial genomes

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Marine Life Science & Technology 2023年第3期5卷 326-336页

作者： Yu Wang yi Yang Lingfeng kong Takenori Sasaki Qi Li Key Laboratory of Mariculture Ministry of EducationOcean University of ChinaQingdao 266003China Laboratory for Marine Fisheries Science and Food Production Processes Laoshan LaboratoryQingdao 266237China The University Museum The University of TokyoTokyo 113-0033Japan

Despite signifcant advances in the phylogenomics of bivalves over the past decade,the higher-level phylogeny of Imparidentia(a superorder of Heterodonta)remains ***,a total of fve new mitochondrial sequences(Chama asperella,Chama limbula,Chama dunkeri,Barnea manilensis and Ctena divergens)was added to provide resolution in nodes that required additional *** the monophyly of Lucinida remains less clear,the results revealed the overall backbone of the Imparidentia tree and the monophyly of ***,most relationships among the fve major Imparidentia lineages—Lucinida,Cardiida,Adapedonta,Myida and Venerida—were addressed with a well-supported *** relationships of Imparidentia recovered Lucinidae as the sister group to all remaining imparidentian *** is a sister group to other imparidentian bivalves(except Lucinidae species)which is split into Cardiida,Adapedonta and the divergent clade of *** was comprised of Venerida and Myida,the former of which now also contains Chamidae as the sister group to all the remaining venerid ***,molecular divergence times were inferred by calibrating nine nodes in the Imparidentia tree of life by extinct *** origin of these major clades ranged from Ordovician to Permian with the diversifcation through the Palaeozoic to ***,the results obtained in this study demonstrate a better-resolved Imparidentia phylogeny based on mitochondrial genomes.

关键词： Imparidentia Phylogeny Mitochondrial genome

An improved technology for eliminating nondeterministic latency in the L1 trigger system

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Nuclear Science and Techniques 2013年第2期24卷 47-52页

作者： DU Zhongwei SU Hong kong Jie ZHAO Xingwen QIAN yi State Key Laboratory of Particle Detection & Electronics University of Science and technology of China Institute of Modern Physics Chinese Academy of Sciences

Gamma Ray Array Detector(GRAD) is one of external target facility subsystems in the Cooling Storage Ring on the Heavy Ion Research Facility at Lanzhou(HIRFL-CSR).The trigger subsystem of GRAD is required to make a fast L1 trigger decision with a fixed latency for the data *** the hit signals from the detector are asynchronous with the local clock of the trigger system,a nondeterministic latency(the value changes between zero and one clock period) is generated when the synchronous receivers of the conventional trigger system process the hit *** this paper,an improved trigger logic based on a field-programmable gate array is developed,and comprised of zero-delay broadening circuits as receivers and an improved adding circuit designed for the new *** simulation and experimental measurement have been *** with the conventional trigger logic,the improved trigger logic has the advantage of eliminating the nondeterministic latency and reducing the total processing latency.

关键词：触发系统延迟不确定性 HIRFL-CSR 现场可编程门阵列重离子研究装置技术时钟周期

Strategic games on a hierarchical network model

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2008年第2期9卷 271-278页

作者： yi-xiao LI Xiao-gang JIN Fan-sheng kong Hui-lan LUO Institute of Artificial Intelligence Zhejiang University Hangzhou 310027 China Ningbo Institute of Technology Zhejiang University Ningbo 315100 China

Among complex network models,the hierarchical network model is the one most close to such real networks as world trade web,metabolic network,WWW,actor network,and so *** has not only the property of power-law degree distribution,but also the scaling clustering coefficient property which Barabási-Albert(BA)model does not *** model is a model of network growth based on growth and preferential attachment,showing the scale-free degree distribution *** this paper,we study the evolution of cooperation on a hierarchical network model,adopting the prisoner's dilemma(PD)game and snowdrift game(SG)as metaphors of the interplay between connected *** model provides a unifying framework for the emergence of *** interestingly,we found that on hierarchical model,there is no sign of cooperation for PD game,while the fre-quency of cooperation decreases as the common benefit decreases for *** comparing the scaling clustering coefficient prop-erties of the hierarchical network model with that of BA model,we found that the former amplifies the effect of *** different performances of PD game and SG on complex network,we also found that common benefit leads to cooperation in the *** our study may shed light on the emergence of cooperation in both natural and social environments.

关键词： Complex network Hierarchical network model Barabási-Albert (BA) model Prisoner's dilemma (PD) game Snowdrift game (SG)

Genome mining of multi-substituted alkylresorcinols from a hybrid highly reducing-and typeⅢ-polyketide pathway

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Chinese Chemical Letters 2024年第7期35卷 345-349页

作者： Huaran Zhang Yuting Huang yingjie Tang Dekun kong yi Zou College of Pharmaceutical Sciences Southwest UniversityChongqing 400715China

Fungal alkylresorcinols are a class of polyketides,which are commonly synthesized by the hybridization of highly reducing polyketide synthase(hrPKS)with non-reducing polyketide synthase(nrPKS).In this study,we identified and demonstrated a new assembly model for synthesizing alkylresorcinol(scirpilin A,1),which was accomplished by collaboration of a hrPKS(FscA)and a typeⅢPKS(FscB).Furthermore,three post-tailoring enzymes(FscC,FscD,and FscE)act iteratively on 1 skeleton,including successive 14e-oxidation of inert carbons,di-halogenation,and di-methylation,to form highly oxidized and multisubstituted *** work presents an unusual synthesis manner of alkylresorcinols,sheds light on the collaborative mechanism between hrPKS and typeⅢPKS and provides three valuable enzymatic catalysts for the tailoring of alkylresorcinol family natural products in future.

关键词： Genome mining Alkylresorcinol hrPKS TypeⅢPKS Iterative catalysis

A highly efficient way to recycle inactive stereoisomers of Bedaquiline into two previous intermediates via base-catalyzed C_(sp3)–C_(sp3) bond cleavage

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Chinese Chemical Letters 2015年第6期26卷 790-792页

作者： De-Long kong Ye Huang Lai-Yang ren Wen-Hua Feng Department of New Drug Research and Development Institute of Materia MedicaChinese Academy of Medical Sciences & Peking Union Medical College

Bedaquiline is a new medicine for pulmonary multi-drug resistant tuberculosis （MDR-TB）, which is a pure enantiomer with two chiral centers. The current industrial preparation process requires the separation of active Bedaquiline from a mixture of four isomers. Obviously, direct dispose of the other three undesired stereoisomers will cause significant waste and increase the unnecessary cost of production. Here, we developed an efficient, facile and scalable process for recycling the inactive stereoisomers of Bedaquiline. All these inactive stereoisomers could be recycled by their conversion to two important intermediates in the Bedaquiline synthesis via a base-catalyzed Csp3-Csp3 bond cleavage of a benzyl alcohol intermediate. And the precise conditions and mechanism of the base-catalyzed cleavage reaction were discussed.

关键词： Bedaquiline Inactive stereoisomers RecycleCsp3-Csp3 cleavage

A Strategy to Find Novel Candidate DKAs Inhibitors Using Modified QSAR Model with Favorable Druggability Properties

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Chemical Research in Chinese Universities 2019年第6期35卷 1111-1118页

作者： ZHANG Xiaoyi NIU Wenling TANG Tang HOU Chengfei GUO Yajie kong ren College of Life Science and Bioengineering Beijing University of TechnologyBeijing 100124P.R.China Institute of Bioinfotmatics and Medical Engineering School of Electrical and Information EngineeringJiangsu University of TechnologyChangzhou 213016P.R.China

The study dealed with quantitative structure-activity relationship(QSAR)to explore the important features of diketo acid(DKA)derivatives for exerting potent HIV-1 integrase inhibitors activity.A three-step screening method was proposed to choose ***,additional descriptors were used in the CoMFA and ***,a modified CoMSIA m7 model,constructed by adding Csp^2_03_F descriptor,showed better predictive *** parameters(Q^2 and R^2)for the models were 0.722 and 0.925,*** addition,external validation for the models using a test group revealed R^2pred=*** maps analysis defined favored and disfavored regions of the compounds,and two new compounds with the descriptor structure were designed with better activities than Raltegravir(RAL),well drug-likeness and low *** research provides a base for further DKA development.

关键词： Diketo acid(DKA) Modified quantitative structure-activity relationship(QSAR) Autodock Drug design Descriptor screening

Associations between body mass index in different childhood age periods and hyperuricemia in young adulthood:the China Health and Nutrition Survey cohort study

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World Journal of Pediatrics 2022年第10期18卷 680-686页

作者： yi Qian Ya-Wei kong Nai-Jun Wan yin-Kun Yan Department of Pediatrics Beijing Jishuitan Hospital68 Huinanbei RoadBeijing 100096China Center for Non-Communicable Disease Management Beijing Children’s HospitalCapital Medical UniversityNational Center for Children’s Health45 Nanlishi RoadBeijing 100045China

Background Few studies have evaluated the specific age period in childhood when the association of body mass index with adult hyperuricemia begins to be *** study aimed to examine the associations between body mass index in different childhood age periods and the risk of adult hyperuricemia in *** The study cohort from the China Health and Nutrition Survey included 676 participants who were aged≥18 years and had data on uric acid in 2009 with at least one measurement of body mass index in childhood surveys before *** were 357,365,358,427,and 432 observations in childhood age groups of≤5 years,6–9 years,10–12 years,13–15 years,and 16–18 years,*** mass index Z score was calculated based on 2000 Center for Disease Control and Prevention growth charts for the United *** Childhood body mass index Z scores measured at age≤5 years,6–9 years,10–12 years,and 13–15 years had no statistical association with adult uric *** comparison,childhood body mass index Z scores measured at age 16–18 years were significantly associated with adult uric acid(β=11.539,P=0.007),and the strength of association was stronger in girls(β=18.565,P=0.002)than in boys(β=9.209,P=0.087).In addition,childhood body mass index Z scores measured at age 16–18 years were significantly associated with an increased risk of adult hyperuricemia(odds ratio=1.323,95%confidence interval=1.003–1.746,P=0.048),but not for other age *** The association between childhood body mass index and young adulthood hyperuricemia was influenced by childhood age.

关键词： Age Body mass index Children Hyperuricemia Obesity