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Gene expression profiles of human adipose-derived mesenchymal stem cells dynamically seeded on clinically available processed nerve allografts and collagen nerve guides

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Neural Regeneration Research 2021年第8期16卷 1613-1621页

作者： Femke Mathot Nadia Rbia Roman Thaler Allan B.Dietz Andre Jvan wijnen Allen T.Bishop Alexander Y.Shin Department of Orthopedic Surgery Mayo ClinicRochesterMNUSA Department of Plastic Surgery RadboudumcNijmegenThe Netherlands Department of Dermatology Erasmus Medical CenterRotterdamThe Netherlands Department of Biochemistry and Molecular Biology Mayo ClinicRochesterMNUSA Department of Laboratory Medicine and Pathology Mayo ClinicRochesterMNUSA

It was hypothesized that mesenchymal stem cells(MSCs) could provide necessary trophic factors when seeded onto the surfaces of commonly used nerve graft substitutes. We aimed to determine the gene expression of MSCs when influenced by Avance■ Nerve Grafts or Neura Gen■ Nerve Guides. Human adipose-derived MSCs were cultured and dynamically seeded onto 30 Avance■ Nerve Grafts and 30 Neura Gen■ Nerve Guides for 12 hours. At six time points after seeding, quantitative polymerase chain reaction analyses were performed for five samples per group. Neurotrophic [nerve growth factor(NGF), glial cell line-derived neurotrophic factor(GDNF), pleiotrophin(PTN), growth associated protein 43(GAP43) and brain-derived neurotrophic factor(BDNF)], myelination [peripheral myelin protein 22(PMP22) and myelin protein zero(MPZ)], angiogenic [platelet endothelial cell adhesion molecule 1(PECAM1/CD31) and vascular endothelial cell growth factor alpha(VEGFA)], extracellular matrix(ECM) [collagen type alpha I(COL1A1), collagen type alpha III(COL3A1), Fibulin 1(FBLN1) and laminin subunit beta 2(LAMB2)] and cell surface marker cluster of differentiation 96(CD96) gene expression was quantified. Unseeded Avance■ Nerve Grafts and Neura Gen■ Nerve Guides were used to evaluate the baseline gene expression, and unseeded MSCs provided the baseline gene expression of MSCs. The interaction of MSCs with the Avance■ Nerve Grafts led to a short-term upregulation of neurotrophic(NGF, GDNF and BDNF), myelination(PMP22 and MPZ) and angiogenic genes(CD31 and VEGFA) and a long-term upregulation of BDNF, VEGFA and COL1A1. The interaction between MSCs and the Neura Gen■ Nerve Guide led to short term upregulation of neurotrophic(NGF, GDNF and BDNF) myelination(PMP22 and MPZ), angiogenic(CD31 and VEGFA), ECM(COL1A1) and cell surface(CD96) genes and long-term upregulation of neurotrophic(GDNF and BDNF), angiogenic(CD31 and VEGFA), ECM genes(COL1A1, COL3A1, and FBLN1) and cell surface(CD96) genes. Analysis demonstrat

关键词： Avance■Nerve Grafts dynamic seeding mesenchymal stem cell NeuraGen■Nerve Guides peripheral nerve repair qPCR

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阅读障碍风险家庭幼儿与正常幼儿词汇发展对比研究

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中国听力语言康复科学杂志 2017年第5期15卷 325-330页

作者：陈傲 Frank wijnen Charlotte Koster Hugo Schnack 北京语言大学语言康复学院北京100083 Department of Psychiatry Brain Center Rudolf MagnusUniversity Medical Center UtrechtUtrechtthe Netherlands 3584CX Institute of Linguistics Utrecht UniversityUtrechtthe Netherlands 3512JK Center for Language and Cognition Groningen University of GroningenGroningenthe Netherlands 9700AS

目的探索17月龄~35月龄正常发展儿童和阅读障碍高风险家庭幼儿词汇发展路径的差异。方法使用荷兰语版《麦克阿瑟-贝茨沟通发展量表(词汇及句子)》调查荷兰语儿童的词汇水平及两组儿童的词汇类别,即名词、谓词、虚词的词汇量,对比正常儿... 详细信息

目的探索17月龄~35月龄正常发展儿童和阅读障碍高风险家庭幼儿词汇发展路径的差异。方法使用荷兰语版《麦克阿瑟-贝茨沟通发展量表(词汇及句子)》调查荷兰语儿童的词汇水平及两组儿童的词汇类别,即名词、谓词、虚词的词汇量,对比正常儿童与高风险儿童的组间差异。结果两组儿童的整体词汇水平及各语义类别水平差异都在19-20个月时最明显,且高风险组的词汇水平低于正常组。高风险组儿童对虚词的掌握程度低于正常儿童,这一差异持续时间较长。结论阅读障碍高风险幼儿与典型发展幼儿的词汇发展路径不同,幼儿词汇水平也许可以为预测其未来阅读发展提供依据。

关键词：阅读障碍词汇发展早期预测

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Follow-up in patients with a burn-related emergency department visit:a feasibility study

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Burns & Trauma 2017年第4期5卷 260-267页

作者： H. Goei B. F. M. wijnen S. Mans M. A. C. de Jongh C. H. van der Vlies S. Polinder N. E. E. van Loey M. E. van Baar Association of Dutch Burn Centres Burn Centre Maasstad Hospital PO Box 9100 3007 AC Rotterdam the Netherlands Department of Plastic Reconstructive and Hand Surgery MOVE Research Institute VU University Medical Centre Amsterdam the Netherlands Association of Dutch Burn Centres Burn Centre Maasstad Hospital PO Box 9100 3007 AC Rotterdam the Netherlands Department of Health Services Research CAPHRI School of Public Health and Primary Care Maastricht University 6200 MD Maastricht the Netherlands Trauma Centre Brabant Elisabeth-TweeSteden Hospital Tilburg the Netherlands Burn Centre Maasstad Hospital Rotterdam the Netherlands Department of Public Health Erasmus Medical Centre Rotterdam the Netherlands Association of Dutch Burn Centres Red Cross Hospital Beverwijk the Netherlands Association of Dutch Burn Centres Burn Centre Maasstad Hospital PO Box 9100 3007 AC Rotterdam the Netherlands Department of Public Health Erasmus Medical Centre Rotterdam the Netherlands

Background:Data on epidemiology, costs, and outcomes of burn-related injuries presenting at emergency departments (EDs) are scarce. To obtain such information, a questionnaire study with an adequate response rate is imperative. There is evidence that optimized strategies can increase patient participation. However, it is unclear whether this applies to burn patients in an ED setting. The objective of this feasibility study was to optimize and evaluate patient recruitment strategy and follow-up methods in patients with burn injuries presenting at EDs. Methods:In a prospective cohort study with a 6-month follow-up, patients with burn-related injuries attending two large EDs during a 3-month study period were included. Eligible patients were quasi-randomly allocated to a standard or optimized recruitment strategy by week of the ED visit. The standard recruitment strategy consisted of an invitation letter to participate, an informed consent form, a questionnaire, and a franked return envelope. The optimized recruitment strategy was complemented by a stamped returned envelope, monetary incentive, sending a second copy of the questionnaire, and a reminder by telephone in non-responders. Response rates were calculated, and questionnaires were used to assess treatment, costs, and health-related quality of life. Results:A total of 87 patients were included of which 85 were eligible for the follow-up study. There was a higher response rate at 2 months in the optimized versus the standard recruitment strategy (43.6%vs. 20.0%;OR=3.1 (95%CI 1.1–8.8)), although overall response is low. Non-response analyses showed no significant differences in patient, burn injury or treatment characteristics between responders versus non-responders. Conclusions:This study demonstrated that response rates can be increased with an optimized, but more labor-intensive recruitment strategy, although further optimization of recruitment and follow-up is needed. It is feasible to assess epidemiology, tr

关键词： Burns Emergency department Recruitment strategy Response rate Health care survey methods

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癫痫患者治疗经济评价的系统回顾

癫痫杂志 2018年第6期4卷 526-544页

作者： wijnen BFM van Mastrigt GAPG Evers SMAA 李思思高慧慕洁

越来越多的治疗方案和与癫痫相关的高成本促进了癫痫疾病经济评价的发展。因此,检查这些经济评价的可用性、质量并找出潜在的研究差距非常重要。除了研究抗癫痫药物(AEDs)和非药物(例如癫痫手术、生酮饮食、迷走神经刺激)治疗外,本综述... 详细信息

越来越多的治疗方案和与癫痫相关的高成本促进了癫痫疾病经济评价的发展。因此,检查这些经济评价的可用性、质量并找出潜在的研究差距非常重要。除了研究抗癫痫药物(AEDs)和非药物(例如癫痫手术、生酮饮食、迷走神经刺激)治疗外,本综述还考察了所有经济评价的方法学质量。我们使用MEDLINE,EMBASE,NHS经济评价数据库(NHS EED),Econlit,Web of Science和CEA Registry进行了文献检索。此外,还使用了Cochrane评价,Cochrane DARE和Cochrane健康技术评价数据库。为了识别相关研究,我们设计了将预定义的临床检索策略与检索过滤器结合的模式以识别健康经济学相关的研究。以下主题都使用了特别设计的特定检索策略:(1) AEDs;(2)认知缺陷患者;(3)老年患者;(4)癫痫手术;(5)生酮饮食;(6)迷走神经刺激;(7)治疗(非)惊厥性癫痫持续状态。本综述共纳入40篇文献,其中29篇(73%)为有关药物干预的文章。以"健康经济学标准共识"(Consensus health economic criteria,CHEC)评价,所有文章的平均质量评分为81.8%,最低质量评分为21.05%,有五项研究评分为100%。使用综合健康经济评价报告标准(Consolidated health economic evaluation reporting standards,CHEERS),平均质量得分为77.0%,最低为22.7%,有四项研究评定为100%。所有纳入的文章在方法学上存在很大的差异,这阻碍了将信息进行有意义地合并。总的来说,方法学质量是可以接受的,尽管一些研究质量明显比其他研究差。研究之间的异质性强调需要确定一个参考案例(例如应该如何进行癫痫内的经济评价),并就什么为"最佳治疗标准"达成共识。

关键词：癫痫经济评价药物干预非药物干预研究质量

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儿童青少年校园欺凌保护行为问卷的编制及信效度检验

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儿童青少年校园欺凌保护行为问卷的编制及信效度检验

皮质定量MRI参数与复发-缓解型多发性硬化病人的认知状态相关

第二十四届全国心理学学术会议

作者：王梓璇 Lydia Laninga-wijnen Claire F.Garandeau 刘俊升华东师范大学图尔库大学

本研究旨在编制适合我国儿童青少年的校园欺凌保护行为问卷,从而阐明在中国文化背景下保护行为的多样性。研究分两个子研究,研究1以文献分析以及开放式问卷调查结果为基础建构理论模型并搜集行为样例,初步确定测量校园欺凌中儿童青少年... 详细信息

本研究旨在编制适合我国儿童青少年的校园欺凌保护行为问卷,从而阐明在中国文化背景下保护行为的多样性。研究分两个子研究,研究1以文献分析以及开放式问卷调查结果为基础建构理论模型并搜集行为样例,初步确定测量校园欺凌中儿童青少年保护行为的条目。在此基础上,通过对824名(Mage=11.25)儿童青少年施测,进行项目分析和因素分析,确定问卷的最终条目。研究2对1086名(Mage=11.18)儿童青少年进行测试,检验问卷的信度和效度。研究1的结果表明,该问卷最终确定24个条目,包括果断型保护(assertive defending)、攻击型保护(aggressive defending)、安慰受欺凌者(comforting victims)、向权威人士报告(reporting to authority)以及计策型保护(tactical defending)五个因子。五因素模型拟合良好,χ(166)=350.76,p<.001,CFI=.973,TLI=.954,SRMR=.020,RMSEA=.037,90%CI=[.031,.042]。研究2验证了该问卷的信度(α=.78-.93)及其结构效度和效标关联效度。五种保护行为与认知和情感共情、攻击性、社交能力、同伴喜爱和受欺负有不同程度的相关。具体来说,果断型保护行为与认知共情、社交能力呈显著正相关;攻击型保护行为与认知共情、同伴喜爱、社交能力呈显著负相关,与攻击性、受欺负呈显著正相关;安慰受欺凌者与认知共情、情感共情、同伴喜爱和社交能力呈显著正相关,与攻击性呈显著负相关;向权威人士报告与认知共情呈显著正相关;计策型保护行为与受欺负呈显著正相关,与认知共情呈显著负相关。本研究结果表明,中国儿童青少年保护行为包含五个因子,研究所编制的问卷具有良好的信度和效度,可用于校园欺凌事件中儿童青少年保护行为的测评。

关键词：校园欺凌保护行为问卷编制信效度中国青少年

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国际医学放射学杂志 2020年第2期 251-251页

作者： A.van wijnen F.Petrov M.Maiworm S.Frisch C.Foerch E.Hattingen 赵玉婵 Department of Neurology Goethe University

目的本研究评估多参数及基于脑表面定量MRI(qMRI)方法评估复发-缓解型多发性硬化(RRMS)/临床孤立综合征(CIS)病人的皮质损害,并在假设qMRI参数与认知能

关键词： MRI 多发性硬化病状态相关