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Prospects for discriminating Zingiberaceae species in India using DNA barcodes

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Journal of Integrative Plant Biology 2014年第8期56卷 760-773页

作者： Meenakshi Ramaswamy Vinitha Unnikrishnan Suresh Kumar Kizhakkethil Aishwarya Mamiyil Sabu george thomas Plant Molecular Biology Rajiv Gandhi Centre for BiotechnologyThiruvananthapuram Kerala 695014 India Regional Facility for DNA Fingerprinting Rajiv Gandhi Centre for BiotechnologyThiruvananthapuram Kerala 695014 India Department of Botany University of Calicut

We evaluated nine plastid （matK, rbcL, rpoCl, rpoB, rp136-rpsS, ndhJ, trnL-F, tmrnH-psbA, accD） and two nuclear （ITS and ITS2） barcode loci in family Zingiberaceae by analyzing 60 accessions of 20 species belonging to seven genera from India. Bidirectional sequences were recovered for every plastid locus by direct sequencing of polymerase chain reaction （PCR） amplicons in all the accessions tested. However, only 35 （58%） and 4o accessions （66~） yielded ITS and ITS2 sequences, respectively, by direct sequencing. In different bioinformatics analyses, matK and rbcL consistently resolved 15 species （75%） into monophyletic groups and five species into two para- phyletic groups. The 173 ITS sequences, including 138 cloned sequences from 23 accessions, discriminated only 12 species （6o%）, and the remaining species were entered into three paraphyletic groups. Phylogenetic and genealogic analyses of plastid and ITS sequences imply the possible occurrence ofnatural hybridizations in the evolutionary past in giving rise to species paraphyly and intragenomic ITS heterogeneity in the species tested. The results support using matK and rbcL loci for barcoding Zingiberaceae members and highlight the poor utility of iTS and the highly regarded ITS2 in barcoding this family, and also caution against proposing ITS loci for barcoding taxa based on limited sampling.

关键词： Concerted evolution DNA barcoding ITS heterogeneity natural hybridizations Zingiberaceae

A CLINICAL CLASSIFICATION OF HYPERTENSION

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Chinese Medical Journal 2006年第1期119卷 80-83页

作者： george thomas DEPARTMENT OF CARDIOLOGY INDIRA GANDHI CO-OPERATIVE HOSPITAL GANDHI NAGAR KOCHI 682 020 INDIA

Hypertension is a common cardiovascular problem worldwide. As with any other disease it is important to assess the severity of the disease. However the present classification of hypertension by the Joint National Committee in its seventh report （JNC 7） with numerical values staging the severity of hypertension is theoretically correct but difficult to apply in practice （Table 1）. Admittedly this is a step in the right direction with lesser number of stages compared to the sixth report.

关键词： hypertension classification, cfinical treatment outcomes qualitative aspects

Association of rs1568885, rs1813443 and rs4411591 polymorphisms with anti-TNF medication response in Greek patients with Crohn's disease

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World Journal of Gastroenterology 2014年第13期20卷 3609-3614页

作者： Diamantis thomas Maria Gazouli Theodoros Karantanos Stella Rigoglou Georgios Karamanolis Konstantinos Bramis george Zografos george E Theodoropoulos Colorectal Unit 1st Department of Propaedeutic Surgery"Hippokrateio" HospitalSchool of MedicineUniversity of Athens11527 AthensGreece Department of Basic Medical Science Laboratory of BiologySchool of MedicineUniversity of Athens11527 AthensGreece Gastroenterology Unit 2nd Department of Surgery"Aretaieio" HospitalUniversity of AthensAthens 11527Greece

AIM: To investigate the correlation between rs1568885, rs1813443 and rs4411591 polymorphisms and response to infliximab in a cohort of Greek patients with Crohn’s disease (CD).

关键词： Crohn’ s disease, Treatment response, Infliximab, Polymorphisms

GENERATION OF TRANSGENIC MICE FOR IN VIVO DETECTION OF INSULIN-CONTAINING GRANULE EXOCYTOSIS AND QUANTIFICATION OF INSULIN SECRETION

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Journal of Innovative Optical Health Sciences 2009年第4期2卷 397-405页

作者： JINLING LU NATALIA GUSTAVSSON QIMING LI george KRADDA thomas C.SUUDHOF WEIPING HAN Laboratory of Metabolic Medicine Singapore Bioimaging Consortium A*STAR Singapore 138667 Department of Molecular and Cellular Physiology and Howard Hughes Medical Institute Stanford University School of Medicine Palo Alto CA 94304USA Department of Biochemistry Yong Loo Lin School of Medicine National University of Singapore Singapore 117597

Insulin secretion is a complex and highly regulated *** much progress has been made in understanding the cellular mechanisms of insulin secretion and regulation,it remains unclear how conclusions from these studies apply to living *** few studies have been done to address these issues is largely due to the lack of suitable tools in detecting secretory events at high spatial and temporal resolution in *** combined with genetically encoded biosensor,optical imaging is a powerful tool for visualization of molecular events in *** this study,we generated a DNA construct encoding a secretory granule resident protein that is linked with two spectrally separate fluorescent proteins,a highly pH-sensitive green pHluorin on the intra-granular side and a red mCherry in the *** exocytosis of secretory granules,the dim pHluorin inside the acidic secretory granules became highly fluorescent outside the cells at neutral pH,while mCherry fluorescence remained constant in the process,thus allowing ratiometric quantification of insulin secretory ***,mCherry fluorescence enabled tracking the movement of secretory granules in living *** validated this approach in insulin-secreting cells,and generated a transgenic mouse line expressing the optical sensor specifically in pancreaticβ-*** transgenic mice will be a useful tool for future investigations of molecular mechanism of insulin secretion in vitro and in vivo.

关键词： Insulin secretion diabetes optical imaging secretory granule exocytosis.

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World Journal of Gastroenterology 2020年第22期26卷 2931-2947页

作者： Tea L Laursen thomas D Sandahl Konstantin Kazankov Jacob george Henning Gronbæk Department of Hepatology and Gastroenterology Aarhus University HospitalAarhus N DK-8200Denmark Storr Liver Centre Westmead Institute for Medical ResearchWestmead HospitalSydney NSW 2145Australia University of Sydney Sydney NSW 2145Australia

More than five years ago,the treatment of hepatitis C virus infection was revolutionized with the introduction of all-oral direct-acting antiviral(DAA)*** proved highly efficient in curing patients with chronic hepatitis C(CHC),including patients with *** new DAA treatments were alleged to induce significant improvements in clinical outcome and prognosis,but the exact cause of the expected benefit was ***,little was known about how the underlying liver disease would be affected during and after viral *** this review,we describe and discuss the liver-related effects of the new treatments in regards to both pathophysiological aspects,such as macrophage activation,and the time-dependent effects of therapy,with specific emphasis on inflammation,structural liver changes,and liver function,as these factors are all related to morbidity and mortality in CHC *** seems clear that antiviral therapy,especially the achievement of a sustained virologic response has several beneficial effects on liver-related parameters in CHC patients with advanced liver fibrosis or *** seems to be a timedependent effect of DAA therapy with viral clearance and the resolution of liver inflammation followed by more discrete changes in structural liver *** improvements lead to favorable effects on liver function,followed by an improvement in cognitive dysfunction and portal ***,the data provide knowledge on the several beneficial effects of DAA therapy on liverrelated parameters in CHC patients suggesting short-and long-term improvements in the underlying disease with the promise of an improved longterm prognosis.

关键词： Chronic hepatitis C Antiviral treatment Inflammation Liver fibrosis Liver cirrhosis Metabolic liver function Galactose elimination capacity Urea synthesis capacity Portal hypertension Hepatic encephalopathy

Performance Comparison of Deep CNN Models for Detecting Driver’s Distraction

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Computers, Materials & Continua 2021年第9期68卷 4109-4124页

作者： Kathiravan Srinivasan Lalit Garg Debajit Datta Abdulellah A.Alaboudi N.Z.Jhanjhi Rishav Agarwal Anmol george thomas School of Information Technology and Engineering Vellore Institute of TechnologyVellore632014India Faculty of Information and Communication Technology University of MaltaMsida2080MSDMalta School of Computer Science and Engineering Vellore Institute of TechnologyVellore632014India College of Computer Science Shaqra UniversityKingdom of Saudi Arabia School of Computer Science and Engineering SCETaylor’s UniversitySubang Jaya47500Malaysia

According to various worldwide statistics,most car accidents occur solely due to human *** person driving a car needs to be alert,especially when travelling through high traffic volumes that permit high-speed transit since a slight distraction can cause a fatal *** though semiautomated checks,such as speed detecting cameras and speed barriers,are deployed,controlling human errors is an arduous *** key causes of driver’s distraction include drunken driving,conversing with co-passengers,fatigue,and operating gadgets while *** these distractions are accurately predicted,the drivers can be alerted through an alarm ***,this research develops a deep convolutional neural network(deep CNN)models for predicting the reason behind the driver’s *** deep CNN models are trained using numerous images of distracted *** performance of deep CNN models,namely the VGG16,ResNet,and Xception network,is assessed based on the evaluation metrics,such as the precision score,the recall/sensitivity score,the F1 score,and the specificity *** ResNet model outperformed all other models as the best detection model for predicting and accurately determining the drivers’activities.

关键词： Deep-CNN ResNet Xception VGG16 data classification

Bipolarons in Organic Electroluminescence

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Chinese Physics Letters 2010年第10期27卷 183-185页

作者：姜庆云李盛 thomas F. george 孙鑫 Research Center for Quantum Manipulation Department of Physics Fudan University Shanghai 200433 Department of Physics Zhejiang Normal University Jinhua 310004 Office of the Chancellor and Center for Nanoseience Departments of Chemistry 35 Biochemistry and Physics Astronomy University of Missouri-St.Louis St. Louis MO 63121 USA

We investigate the bipolaron channel B＋＋＋ B-- → BX. The dynamical evolutions and transition probabilities of this bipolaron luminescence channel are studied. It is found that this channel avoids the triplet and is able to enhance the efficiency. We suggest that choosing certain polymers and injection structures, in which B is easily formed, can improve the luminescence efficiency. It is believed that these results will motivate further experimental studies about the function of bipolarons in electroluminescence.

关键词：双极化子电致发光动力学演化工作效率喷射结构发光效率光通道聚合物

Identification and Characterization of Genes Responsible for Drought Tolerance in Rice Mediated by Pseudomonas fluorescens

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Rice science 2017年第5期24卷 291-298页

作者： Manjesh SAAKRE Thirthikar Meera BABURAO Abida Puthenpeedikal SALIM Rose Mary FFANCIES Valasala Poothecty ACHUTHAN george thomas Sajeevan Radha SIVARAJAN Centre for Plant Biotechnology and Molecular Biology/Department of Genetics and Plant Breeding/Department of Agronomy College of Horticulture Kerala Agricultural University Vellanikkara Thrissur 680656 Kerala India

Drought is one of the major abiotic stresses which adversely affect crop plants limiting growth and yield *** and functional characterization of drought stress-induced genes has contributed to a better understanding of how plants respond and adapt to the drought *** the present study,differential display technique was employed to study the gene expression of rice plants at the reproductive stage that were subjected to drought stress by withholding water,Pseudomonas fluorescens strain(Pf1) treated plants subjected for drought stress by withholding water and control(well-watered).Differentially expressed c DNAs of six genes(COX1,PKDP,b ZIP1,AP2-EREBP,Hsp20 and COC1) were identified,cloned and ***-time q PCR analysis showed that all the six genes were upregulated in drought-stressed plants treated with *** revealed that the remarkable influence of Pf1 colonization leads to drought tolerance at the reproductive *** results showed that high levels of gene expression in plants lacking adequate water can be remarkably influenced by Pf1 colonization,which might be a key element for induced systemic tolerance by microbes.

关键词： rice drought tolerance Pseudomonas fluorescens differential display reverse transcription polymerase chain reaction quantitative real-time PCR transcript derived fragment

Gastric per-oral endoscopic myotomy: Current status and future directions

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World Journal of Gastroenterology 2019年第21期25卷 2581-2590页

作者： Alexander Podboy Joo Ha Hwang Linda A Nguyen Patricia Garcia thomas A Zikos Afrin Kamal george Triadafilopoulos John O Clarke Division of Gastroenterology and Hepatology Stanford University School of Medicine

Gastroparesis, or symptomatic delayed gastric emptying in the absence of mechanical obstruction, is a challenging and increasingly identified syndrome. Medical options are limited and the only medication approved by the Food and Drug Administration for treatment of gastroparesis is metoclopramide, although other agents are frequently used off label. With this caveat, first-line treatments for gastroparesis include dietary modifications, antiemetics and promotility agents, although these therapies are limited by suboptimal efficacy and significant medication side effects. Treatment of patients that fail first-line treatments represents a significant therapeutic challenge. Recent advances in endoscopic techniques have led to the development of a promising novel endoscopic therapy for gastroparesis via endoscopic pyloromyotomy, also referred to as gastric per-oral endoscopic myotomy or per-oral endoscopic pyloromyotomy. The aim of this article is to review the technical aspects of the per-oral endoscopic myotomy procedure for the treatment of gastroparesis, provide an overview of the currently published literature, and outline potential next directions for the field.

关键词： Gastroparesis Gastric per-oral endoscopic myotomy Per-oral endoscopic pyloromyotomy Myotomy Endoscopic myotomy Peroral endoscopic myotomy Gastric emptying